• Journal of Food Hygiene and Safety
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• v.27 no.3
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• pp.285-289
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• 2012

In this study, the veridical efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite against red sea bream iridovirus (RBIV). A veridical efficacy was determined with the viability of RBIV contacted with the disinfectant in viral stock cultured in fat head minnow cell line. An aquatic disinfectant tablet and RBIV were reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW and HW condition, RBIV was inactivated with 25,000 fold dilutions of an aquatic disinfectant tablet. With the investigation of the antiviral effect of the disinfectant on OM condition, RBIV was inactivated on 22,000 fold dilutions of an aquatic disinfectant tablet. As an aquatic disinfectant tablet possesses veridical efficacy against RBIV, the disinfectant solution can be used to limit the spread of cultured marine fish viral disease.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.347-351
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• 2009

Bruella spp. involves a considerable danger of public health and farm animal industry. In this study, we assessed the disinfection efficacy of alkaline disinfectant solution and three commercial farm disinfectants (quaternary ammonium compound, sodium dichloroisocyanurate, potassium monopersulphate/sodium dichloroisocyanurate) against Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of selected disinfectants following exposure to test bacteria for 30 minutes at $4^{\circ}C$. Disinfectants and test bacteria are diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. Three commercial disinfectant showed excellent antimicrobial activity (up to dilution of $\times200$ in OM treatment). Alkaline disinfectant solution demonstrated favorable bactericidal efficacy against B. abortus (at dilution of $\times20$ in OM treatment). Three commercial farm disinfectants possess excellent efficacy against B. ovis. Alkaline disinfectant solution has lower potency than commercial farm disinfectant but could help to limit the spread of brucellosis.

• Journal of Environmental Health Sciences
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• v.39 no.4
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• pp.376-382
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• 2013

Objectives: The objectives of this study were to identify the virucidal efficacy against the viral hemorrhagic septicemia virus (VHSV) of an aquatic disinfectant tablet composed of calcium hypochlorite. Methods: Virucidal efficacy was determined through the viability of VHSV contacted with the disinfectant in a viral stock cultured in a fathead minnow cell line. An aquatic disinfectant tablet and VHSV were reacted under distilled water (DW), hard water (HW) or organic matter suspension (OM) conditions. Results: Under DW and HW conditions, VHSV was inactivated with 24,000- and 2000-fold dilutions of the aquatic disinfectant tablet, respectively. With the investigation of the antiviral effect of the disinfectant under OM conditions, VHSV was inactivated with a 16,000-fold dilution of the aquatic disinfectant tablet. Conclusions: The results from this study showed that the aquatic disinfectant tablet was a highly effective disinfectant against VHSV. In the future, a controlled field trial is required to determine whether the use of an aquatic disinfectant tablet will be able to reduce VHSV in a cultured marine fish farm.

• Korean Journal of Veterinary Service
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• v.37 no.2
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• pp.105-110
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• 2014

Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED) and causes a considerable economic loss in swine industry. In this study, the virucidal efficacy of the disinfectant composed to n-alkyl-dimethyl-benzyl-ammonium chloride (n-ADBAC) was investigated against PEDV. A virucidal efficacy was determined with the viability of PEDV contacted with the disinfectant in Vero cells. The disinfectant and PEDV were reacted on the hard water (HW) or organic matter suspension (OM) condition. On HW condition, PEDV was inactivated with 50 fold dilutions of the disinfectant. When the antiviral effect on OM condition was evaluated, the antiviral activity of the disinfectant showed on 10 fold dilutions against PEDV. As the disinfectant possesses the virucidal efficacy against PEDV, the disinfectant solution can be used to limit the spread of animal viral diseases.

• Journal of Dairy Science and Biotechnology
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• v.39 no.3
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• pp.104-112
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• 2021

This study was conducted to evaluate the efficacy of commercial disinfectants at inactivating Enterobacter sakazakii (Cronobacter spp.) in water. Disinfectant I contained 6.15% sodium hypochlorite, and disinfectant II contained both 2.25% n-alkyl dimethylbenzyl ammonium chloride and 2.25% n-alkyl ethylbenzyl ammonium chloride. Disinfectant I was added to distilled water to obtain a range of residual chloride concentrations at 50 ppm intervals with a maximum of 1-1,000 ppm. Disinfectant II was prepared at concentrations ranging from 1-200 ppm with 5 ppm intervals. Exposure time for all solutions was 10 min. In total, 58 E. sakazakii (Cronobacter spp.) strains were tested in this study. Nine isolates were obtained from clinical samples, and 49 isolates were obtained from environmental samples. Seven strains (6 clinical and 1 environmental) were able to survive in 100 ppm disinfectant I, and a maximum of 5 ppm of disinfectant II. Fifty one strains (3 clinical and 48 environmental) were not killed in 10 ppm of disinfectant I and 1 ppm of disinfectant II in water. In conclusion, this study demonstrated that clinical E. sakazakii (Cronobacter spp.) strains displayed 5- to 10-fold higher resistance to disinfectants than environmental E. sakazakii (Cronobacter spp.) strains. Disinfectant II, containing quaternary ammonium compounds, was shown to be more potent in inactivating E. sakazakii (Cronobacter spp.) in water used to clean infant formula manufacturing equipment than disinfectant I.

• Environmental Analysis Health and Toxicology
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• v.27
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• pp.2.1-2.4
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• 2012

Objectives: This study is a cross-sectional study to investigate the frequency of humidifier and humidifier disinfectant usage in the general population. Methods: A questionnaire was applied to 94 subjects (46 male, 48 female) from the general population of Gyeonggi Province. The questionnaire consisted of 3 scales (general characteristics, 5 items about humidifier usage, 5 items about humidifier disinfectant usage). Results: Thirty-five (37.2%) of the 94 subjects use a humidifier and humidifier disinfectant usage was found to be 18.1%. The frequency of humidifier usage is 4.8 times per a week and the humidifier disinfectant usage is 2.4 times per a week. Humidifier usage rate was highest in January. and then tended to rise gradually from October. Conclusions: Although this study population was not representative, we can say that approximately 50 percent of the humidifier users use a humidifier disinfectant and the trend of using a humidifier has shown seasonality.

• Particle and aerosol research
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• v.18 no.2
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• pp.37-50
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• 2022

Due to the syndrome coronavirus 2 (SARS-CoV-2) pandemic, the subway passenger cabin should be continuously sterilized. However, a disinfectant such as chlorine is toxic and can lead to different issues to human health. In this paper, we introduced a novel disinfectant based on natural product (Dendropanax morbifera extract). Via ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS), different compounds from Dendropanax morbifera extract showed antivirus potentials. Antimicrobial experiments confirmed that the air-disinfectant containing Dendropanax morbifera can eliminate harmful microorganisms including Gram (-), Gram (+), and yeast within 5 mins. The as-prepared air-disinfectant also showed high antivirus activity against H1N1, HRV, and EV71. Deodorization test also indicates that the as-prepared air-disinfectant can lower the harmful gas such as ammonia and trimethylamine in the atmosphere. To evaluate the potential of air-disinfectant containing Dendropanax morbifera in practical applications, different safety tests including acute oral toxicity, acute skin irritation, and eye irritation were conducted. Results showed that the as-prepared disinfectant did not negatively affect tested animals during these safety investigations.

• Journal of Food Hygiene and Safety
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• v.27 no.3
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• pp.290-294
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• 2012

Vibrio spp. and Streptococcus spp. have caused a considerable disease of farmed fish and economic loss in fish farming and seafood industry. In this study, the efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite was evaluated against V. anguillarum and S. iniae. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. An aquatic disinfectant tablet and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. V. anguillarum on the DW, HW and OM condition was completely inactivated with 16,000 15,000 and 13,000 fold dilutions of the disinfectant, respectively. On the DW, HW and OM condition, S. iniae was absolutely inactivated with 17,000 16,000 and 14,000 fold dilutions of the disinfectant, respectively. As an aquatic disinfectant tablet possesses bactericidal efficacy against fish pathogenic bacteria such as V. anguillarum and S. iniae this disinfectant solution can be used to control the spread of fish infective bacterial diseases.

• Journal of Food Hygiene and Safety
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• v.31 no.6
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• pp.471-475
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• 2016

Salmonella Typhimurium (S. Typhimurium) is one of the common food pathogens which may cause gastroenteritidis in human and animals. The microorganism also causes the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a powder disinfectant containing phosphate compounds as a main ingredient, was evaluated against S. Typhimurium. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW and OM conditions, the bactericidal activity of the disinfectant against S. Typhimurium was 4- and 2-fold dilutions, respectively. As the disinfectant composed to phosphate compounds possesses bactericidal efficacy against pathogenic bacteria such as S. Typhimurium, the powder disinfectant can be used to control the spread of bacterial diseases.

• Journal of Korean Society of Water and Wastewater
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• v.30 no.1
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• pp.9-17
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• 2016

Disinfectant/oxidation process is a crucial process in water treatment for supplying safe drinking water. Chlorination is still widely used for water treatment area due to its effectiveness on microbial inactivation and economic feasibility. Recently, disinfection concern in marine environment is increasing, for example, movement of hazardous marine organism due to ballast water, marine environmental degradation due to power plant cooling water discharge, and increase of the amount of disinfectant in the offshore plant. It is needed to conduct the assessment of disinfectant behavior and the development of disinfectant prediction model in seawater. The appropriate prediction model for disinfectant behavior is not yet provided. The objective of the study is to develop chlorine decay model in seawater. Various model types were applied to develop the seawater chlorine decay model, such as first order decay model, EPA model, and two-phase model. The model simulation indicated that chlorine decay in seawater is influenced by both organic and inorganic matter in seawater. While inorganic matter has a negative correlation with the chlorine decay, organic matter has a positive correlation with the chlorine decay.