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Antimicrobial activities of Bacillus subtilis DS660 and Paenibacillus polymyxa DS842 (Bacillus subtilis DS660과 Paenibacillus polymyxa DS842의 항균활성)

  • Lee, Da-Sol;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.354-361
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    • 2018
  • This study was carried out to isolate soil bacteria with antimicrobial activity and evaluate antimicrobial substances produced by isolated bacteria. Among many isolates Bacillus subtilis DS660 and Paenibacillus polymyxa DS842 showed high antimicrobial activities against 6 species of microbial residents on human skin and 3 species of pathogenic bacteria. DS660 and DS842 showed 15.3~26.8 and 11.3~27.5 mm of inhibition zone diameter, respectively on nutrient agar medium against most target bacteria and fungi. DS660 and DS842 produced $57{\pm}8$ and $170{\pm}15{\mu}mol/ml$ of siderophore, respectively as an antimicrobial substance. Analysis of ethyl acetate extract of culture supernatants of DS660 and DS842 suggested production of glycolipid biosurfactant which reduced surface tension of culture supernatant of DS660 and DS842 from 60.0 to 40.3 and 30.3 mN/m, respectively. DS660 and DS842 also showed $169.2{\pm}9.9$ and $357.2{\pm}13.7nmol/min/mg$ protein of ${\beta}-1,3$-glucanase activity, respectively, and hydrolyzed cell wall components of 3 bacterial species. These results suggest that B. subtilis DS660 and P. polymyxa DS842 may be utilized as an environment-friendly biocontrol agent against some skin microbes and pathogenic bacteria.

Performance Analysis of MFSK-MDPSK/DS-CDMa System Employing Diversity Techniquies and Error Coding Technique in Nakagami Fading Environment (나카가미 페이딩 환경하에서 다이버시티기법과 에러 정정부호화 기법을 채용한 MFSK-MDPSK/DS-CDMA 시스템의 성능 분석)

  • 양원일;강희조
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.4
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    • pp.553-564
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    • 2000
  • In this thesis, mobile communication channel with multi-path fading which is major factor of performance degradation of DS-CDMA system has been considered. An MRC diversity and error correcting coding technique have been used to improve performance of MFSK-MDPSK/DS-CDMA system. The MFSK-MDPSK/DS-CDMA system performance has been obtained quantitatively by the numerical analysis. the modulations under consideration are noncoherent M-ary frequency shift keying(MFSK) and an MFSK based joint frequency phase modulation utilizing MFSK/DS-CDMA and MDPSK-DS-CDMA systems the MFSK-MDPSK/DS-CDMA system obtain a better performance . Also, the error performance of MFSK-MDPSK/DS-CDMA system in Nakagami fading environments is improved by increasing the number of diversity branch and coding gain.

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Implementation of DS/SS(FM-DS/SS) system using FM (FM을 이용한 DS/SS(FM-DS/SS) 시스템의 구현)

  • 정명덕;박지언;변건식
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.9 no.1
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    • pp.98-107
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    • 1998
  • For implementation of FM-DS/SS system. This paper has analyzed SO(Synchronous Oscillator) being oscillated by the motivation of injected signal. Transmitter has adopted FM-DS/SS modulation method using RF output-signal of FM. Receiver is used SO to demodulation of FM-DS/SS and applied sliding correlator for synchronization of PN clock. As a result of the inspection, SO presented stable lock ability in spite of doppler apperance and proved the synchronous properties of it in the FM-DS/SS system.

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Antibacterial activity of isolated bacteria against Propionibacterium acnes causing acne vulgaris (여드름을 유발하는 Propionibacterium acnes에 대한 분리 세균들의 항균활성)

  • Lee, Da-Sol;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.272-279
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    • 2018
  • This study was carried out to evaluate antimicrobial activity of isolated bacteria from various soils against two strains of Propionibacterium acnes causing acne vulgaris. Among several hundreds of bacterial strains, Paenibacillus elgii DS381, Paenibacillus elgii DS1515, Burkholderia gladioli DS518, and Streptomyces lienomycini DS620 showed high antimicrobial activities against the strains of P. acnes. All isolated bacteria showed 15.5 to 34.3 mm inhibition zone diameter in an agar well diffusion test, and especially DS620 showed the highest inhibition zone diameters (28.3~34.3 mm). Antibacterial substances were expected as lipopeptide (pelgipeptin and paenipeptin) from strains DS381 and DS1515, protease from DS518, and anthracycline antibiotic (daunomycinone) from DS620, and all these showed very low minimum inhibitory concentration [DS381 and DS1515 (0.078 mg/ml), DS518 (0.312 mg/ml), DS620 (0.000078 mg/ml)] against P. acnes. These antibacterial substances could completely kill P. acnes within 24 h in a time-kill assay. These results suggest that antibacterial substances produced by these bacteria may be utilized as useful antimicrobial agent against P. acnes and treatment medicine for acne vulgaris.

Comparison and Analysis of the MC DS-CDMA/MPSK and DS-CDMA/MPSK Systems in Nakagami Fading (나카가미 감쇄에서 MC DS-CDMA/MPSK와 DS-CDMA/MPSK 시스템 비교 분석)

  • 이정도;강희조
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.6
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    • pp.951-958
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    • 1999
  • Multicarrier modulation or orthogonal frequency division multiplexing(OFDM) is a promising technique for mobile communications systems, since it has a strong immunity to multipath fading without employing complicated adaptive equalization. In this paper, performance of MC DS-CDMA/MPSK(multi-carrier direct sequence-code division multiple access/mary phase shift keying) and DS-CDMA/MPSK(direct sequence-code division multiple access/mary phase shift keying) with coherent detection for a CDMA system over Nakagami fading channel analysis. For a given bandwidth, it is shown that MC DS-CDMA/MPSK eventually outperforms DS-CDMA MPSK with random sequences.

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Throughput Analysis of Multi-Carrier DS-CDMA System in Rayleigh Fading Channels (레일리 페이딩 채널에서 Multi-Carrier DS-CDMA 시스템의 Throughput 해석)

  • 김영철;노재성;강희조;조성준
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.5 no.6
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    • pp.1066-1072
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    • 2001
  • ALOHA Multi-Carrier DS-CDMA systems in the multipath Rayleigh fading channel. The throughput of two systems are compared according to the length of a packet in the fast Rayleigh and the slow Rayleigh multipath fading. As a result, we have known that Multi-Carrier DS-CDMA system has less capacity than wideband DS-CDMA system in the same bandwidth, but throughput of Multi-Carrier DS-CDMA system is higher than that of wideband DS-CDMA system. And in Multi-Carrier DS-CDMA systems, higher throughput can be obtained through shortening the length of packet in the fast Rayleigh fading, and it is efficient to control the length of a packet adequately or increase the system capacity of Multi-Carrier DS-CDMA system in the slow Rayleigh fading.

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SCTS Conformance Test for OMA DS Standard for an Embedded Data Synchronization Gateway (임베디드 자료동기화 게이트웨이를 위한 OMA DS 표준 SCTS 적합성 테스트)

  • Pak, Ju Geon;Park, Kee Hyun
    • IEMEK Journal of Embedded Systems and Applications
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    • v.5 no.4
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    • pp.217-224
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    • 2010
  • Nowadays, people perform their tasks anywhere anytime using their mobile devices. For this reason, data synchronization (DS) between mobile devices and a central server has become one of the most essential technologies in mobile environments. Currently, several mobile DS protocols are proposed and used. However, the existing DS protocols cannot guarantee interoperability between them. To solve the problem, an embedded DS gateway has been developed in our previous study. The gateway runs on a Windows Mobile-based emulator. It converts data on a mobile device into common data specified by OMA DS standard protocol and vice versa. The embedded gateway has been built to support the OMA DS standard protocol. In order to verify that the embedded gateway conforms to the OMA DS standard protocol, two kinds of OMA conformance tests have to be conducted - interoperability test with an OMA DS-based server and conformance test with SCTS (SyncML Conformance Test Suit). In this paper, some parts of the gateway previously built are modified and the modified gateway is installed on a Windows Mobile-based smart phone. And the interoperability test and the conformance test with the SCTS are conducted. The results of the tests show that the embedded DS gateway operates properly on the Windows Mobile-based smart phone and that the gateway passes the tests, verifying its conformity to the OMA DS standard protocol. In addition, DS performance tests show that DS delay times between a real smart phone and a DS server increase gently as the number of DS data increases. In other words, the embedded DS gateway built in this paper can be used for a real smart phone at a reasonable performance cost.

Throughput Analysis of Multi-Carrier DS-CDMA System in Rayleigh Fading Channels (레일리 페이딩 채널에서 Multi-Carrier DS-CDMA 시스템의 Throughput 해석)

  • 김영철;노재성;강희조;조성준
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2001.10a
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    • pp.675-679
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    • 2001
  • In this paper, throughput is derived for slotted ALOHA wideband DS-CDMA and slotted ALOHA Multi-Carrier DS-CDMA systems in the multipath Rayleigh fading channel. The throughput of two systems are compared according to the length of a packet in the fast Rayleigh and the slow Rayleigh multipath fading. As a result, we have known that Multi-Carrier DS-CDMA system has less capacity than wideband DS-CDMA system in the same bandwidth, but throughput of Multi-Carrier DS-CDMA system is higher than that of wideband DS-CDMA system. And in Multi-Carrier DS-CDMA systems, higher throughput can be obtained through shortening the length of packet in the fast Rayleigh fading and it is efficient to control the length of a packet adequately or increase the system capacity of Multi-Carrier DS-CDMA system in the slow Rayleigh fading.

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Bifidobacterium bifidum DS0908 and Bifidobacterium longum DS0950 Culture-Supernatants Ameliorate Obesity-Related Characteristics in Mice with High-Fat Diet-Induced Obesity

  • M. Shamim Rahman;Youri Lee;Doo-Sang Park;Yong-Sik Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.1
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    • pp.96-105
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    • 2023
  • Probiotic supplements have promising therapeutic effects on chronic diseases. In this study, we demonstrated the anti-obesity effects of two potential probiotics, Bifidobacterium bifidum DS0908 (DS0908) and Bifidobacterium longum DS0950 (DS0950). Treatment with DS0908 and DS0950 postbiotics significantly induced the expression of the brown adipocyte-specific markers UCP1, PPARγ, PGC1α, PRDM16 and beige adipocyte-specific markers CD137, FGF21, P2RX5, and COX2 in C3H10T1/2 mesenchymal stem cells (MSCs). In mice with high-fat diet (HFD)-induced obesity, both potential probiotics and postbiotics noticeably reduced body weight and epididymal fat accumulation without affecting food intake. DS0908 and DS0950 also improved insulin sensitivity and glucose use in mice with HFD-induced obesity. In addition, DS0908 and DS0950 improved the plasma lipid profile, proved by reduced triglyceride, low-density lipoprotein, and cholesterol levels. Furthermore, DS0908 and DS0950 improved mitochondrial respiratory function, confirmed by the high expression of oxidative phosphorylation proteins, during thermogenesis induction in the visceral and epididymal fat in mice with HFD-induced obesity. Notably, the physiological and metabolic changes were more significant after treatment with potential probiotic culture-supernatants than those with the bacterial pellet. Finally, gene knockdown and co-treatment with inhibitor-mediated mechanistic analyses showed that both DS0908 and DS0950 exerted anti-obesity-related effects via the PKA/p38 MAPK signaling activation in C3H10T1/2 MSCs. Our observations suggest that DS0908 and DS0950 could potentially alleviate obesity as dietary supplements.

Insect Pest Control Technique Using dsRNA (dsRNA를 이용한 해충방제 기술)

  • Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.2
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    • pp.153-164
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    • 2017
  • Gene silencing using double-stranded RNA (dsRNA) has been widely used in functional genomics in biological organisms. Its principle stems from RNA interference (RNAi), a post-transcriptional control of gene expression. Suppression of specific gene expression using dsRNA may give significant lethal effect. Insect pest control exploits this molecular process to develop novel insecticides using specific dsRNAs. This review explains core principles of RNAi using dsRNA. Then it illustrates various examples to control insect pests using dsRNAs. It also discusses limitations to control insect pests using dsRNAs. Finally, it provides several breakthroughs to develop dsRNA insecticides.