• Environmental Engineering Research
• /
• v.12 no.5
• /
• pp.231-237
• /
• 2007

The microbial eco-physiology has been the vital key of microbial ecological research. Unfortunately, available methods for direct identity of microorganisms and for the investigation of their activity in complicated community dynamics are limited. In this study, metagenomics was considered as a promising functional genomics tool for improving our understanding of microbial eco-physiology. Its potential applications and challenges were also reviewed. Because of tremendous diversity in microbial populations in environment, sequence analysis for whole metagenomic libraries from environmental samples seems to be unrealistic to most of environmental engineering researchers. When a target function is of interest, however, sequence analysis for whole metagenomic libraries would not be necessary. For this case, nucleic acids of active populations of interest can be selectively gained using another cutting-edge functional genomic tool, SIP (stable isotope probing) technique. If functional genomes isolated by SIP can be transferred into metagenomic library, sequence analysis for such selected functional genomes would be feasible because the reduced size of clone library may become adequate for sequencing analysis. Herein, integration of metagenomics with SIP was suggested as a novel functional genomics approach to study microbial eco-physiology in environment.

• Journal of Environmental Science International
• /
• v.32 no.12
• /
• pp.955-964
• /
• 2023

This study was conducted to obtain basic data on efficient and eco-friendly crop cultivation for urban residents who enjoy urban agriculture as a hobby or leisure activity. We planted strawberry(Fragaria x ananassa)(S) and kidney bean (Phaseolus vulgaris var. humilis)(K) in different ratios to analyze the differences in soil environment, growth, physiology, and productivity, and to investigate the effects. Strawberry growth was optimal with S1K2 ratio, whereas the S1K1 ratio treatment showed the highest levels of physiology and productivity. In terms of growth and physiology, kidney beans tended to perform best in S1K2 treatment. The average number of productive pods was two, with a highest average value of 2.3 being recorded in S2K1 treatment planted with a high percentage of strawberries. In terms of growth, physiology, and prodctivity our findings indicate that it would be desirable to plant starwberries and kidney bean in a 1:1 ratio. However, considering the environmental characteristics of walls and rooftops, it is necessary to effectively manage crops suitable for these conditions. Furthermore, additional studies should be conducted to analyze the quality of fruits and seeds producted, both qualitatively and quantitatively in the future.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.120-120
• /
• 2022

Agrivoltaic System (AVS) was introduced with the concept that it could generate electricity by using the extra light remain after crops use for photosynthesis in farm, which can earn additional income. However, crop yield was declined under the AVS condition due to the decrease in light energy. In the past, many researchers have been studied about crop states under shading conditions. However, the phenomenon of partial shading such as under the AVS is not well studied. In this study, to figure out the response of crop under the different light conditions, the electron transport rate (ETR) and non-photochemical quenching (NPQ) of rice was investigated using the chlorophyll fluorescence measurement. Also, physiological changes of crops under the shading conditions were investigated. The growth experiment under partial shading under AVS and overall shading which made of 35% shade cloth was conducted to understand the eco-physiological responses of rice to light in terms of the photosynthesis. Under the shading conditions, SPAD value and chlorophyll contents were higher, but the leaf thickness was lower than control. The overall shading condition show lower ETR than others during the growing season. In contrast, NPQ was higher than other treatments. This means the available light energy cannot contribute to photosynthesis under the shading condition.

• Journal of Forest and Environmental Science
• /
• v.31 no.3
• /
• pp.214-224
• /
• 2015

The economy of India and so also of many Asian countries depends on bamboos and their uses are not only in domestic items but also in rural housing and raw materials to several industries and germplasm characterization is an important link between the conservation and utilization of plant genetic resources. Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering coupled with different biotic agencies and environmental factors. Identification and genetic relationships among accessions of Thamnocalamus falconeri were investigated using morphology and random amplified polymorphic DNAs (RAPD) technique. Analysis started by using 51 vegetative characters and forty two 10-mer primers that allowed us to distinguish different genotypes hailing from different eco- zones of Garhwal Himalayas (India). The selected primers (12) were used for identification and for establishing a profiling system to estimate genetic diversity. A total of 79.33% polymorphism was estimated by using 12 selected primers. The genetic similar analysis was conducted based on binary digits i.e. presence (1) or absence (0) of bands, which revealed a wide range of variability among the species whereas genetic relatedness was quite high based on vegetative characters. Cluster analysis clearly showed two major clusters for both of the markers viz. morphology and RAPD belonging to 10 accessions of T. falconeri. Two major clusters were further divided into minor clusters. Cluster based on RAPD marker showed grouping of accessions of closed locality whereas analogy was reported for vegetative traits. The RAPD technique has the potential for use in species identification and genetic relationships studies of bamboo for breeding program.

• Journal of Fisheries and Marine Sciences Education
• /
• v.23 no.2
• /
• pp.153-161
• /
• 2011

Creating standards on thermal environment has been organized traditionally based on the youth and the manhood who are in mainly active layer of a society. However, traditional creating standards have differences from the physiology of the aged who have weak physical ability than younger person. As a result, it causes a health problem of the aged. Therefore, In this study, we had a basic study to create a comfortable thermal environment which had considered to a physical ability and a physiology of the aged, and build a ecological residence space to maintain health. We had several experiments with the aged; Experiment, Comfort Sensation Vote, Mean Skin Temperature and Analyzing HRV. The result have following by: 1)For the aged, the summer recommend temperature, $26^{\circ}C$, is appropriate within first 30 ~ 40 minutes, but it should be increasing the temperature after that time. 2) By considering PMV status and thermal feeling of the aged, they are prefer to higher temperature than normal setting of air-condition system. 3) In the condition of the summer recommend temperature, $26^{\circ}C$, they had answered in neutral or comfort with the comfort sensation vote. However, we had figure out that they had stress in a lower temperature by analyzing the result of HRV.

• Korean Journal of Ecology and Environment
• /
• v.54 no.2
• /
• pp.79-86
• /
• 2021

In this study, the distribution and habitat characteristics of Odontobutis obscura were investigated. It is a freshwater fish species which belongs to the Class I Endangered species as specified by the Ministry of Environment of South Korea. Sampling was conducted in the Sanyang Stream watershed between April and August of 2016. The Sanyang Stream watershed includes the Sanyang and Gucheon Streams, and their tributaries. Odontobutis obscura was caught at every sampling site, except sites 1~3, which are located in the lower part of the Sanyang Stream. Its habitat range extended from the headwaters to the lower parts of the stream that were not impacted by the South Sea. Salinity was the major factor limiting the distribution of O. obscura in the Sanyang Stream watershed. All individuals of O. obscura were caught in areas where the substrate composition was over 50% gravel. Furthermore, the substrate composition seems to be the most important habitat factor affecting spawning of O. obscura. Ecological studies of O. obscura are scarce, and this study is the first to report a detailed distribution for the species. Further studies on the physiology and ecology of O. obscura are essential for establishing preservation strategies for this endangered species.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.6
• /
• pp.827-831
• /
• 2007

Myostatin is a member of the transforming growth factor-${\beta}$(TGF-${\beta}$ super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generalized increase in muscle mass in double muscled cattle breeds. To understand the biochemistry and physiology of the Chinese Yellow bovine myostatin gene, we report here for the first time expression of the gene in Escherichia coli (E. coli). Primers of the myostatin gene of Chinese Yellow Cattle were designed on the basis of the reported bovine myostatin mRNA sequence (Gen-Bank Accession No. NM005259) and optimized for E. coli codon usage. XhoI and EcoRI restriction enzyme sites were incorporated in the primers, and then cloning vector and expression vector were constructed in a different host bacterium. The expressed protein had a molecule mass of about 16 kDa as determined by SDS-PAGE under reducing conditions. The expressed protein reacted specifically with myostatin monoclonal antibody on immunoblots. Our studies should lead to the investigation of the differences in myostatin genes of various cattle and could benefit human health and food animal agriculture.

• Fashion & Textile Research Journal
• /
• v.8 no.1
• /
• pp.129-133
• /
• 2006

To develope a high value-added fiber products which is useful in the human body physiology, the Macban stone was deposited on the PET fabric by sputtering and its effects were investigated. Then, a Macban stone target was prepared for sputtering treatment and treated on the PET fabric by RF sputtering process. After treatment, surface observation by SEM, far-infrared emissivity and emissive power, the fastness to washing and light, bacteriostatic rate, electrostatic, drape stiffness, and breaking strength of PET fabric were investigated. From these investigation, the following conclusions were obtained. 1) The Macban stone was able to deposit on the PET fabric, by the RF sputtering treatment which is eco-friendly dry textile finishing. 2) The far-infrared emissivity and emissive power of sputtered PET fabrics were increased. 3) When PET fabric was treated by sputtering with Macban stone, the amount of deposited Macban stone increased with increasing treatment time and it was deposited on the fabric surface firmly. 4) The bacteriostatic rate of sputtered PET fabrics was about 20%. 5) The electrostatic charge of fiber surface was reduced by sputtering. 6) The fastness washing to light of dyed fabric were improved by the deposition of Macban stone, but the breaking strength was not changed by sputtering. 7) The drape stiffness of sputtered PET fabrics increased with increasing treatment time.

• Weed & Turfgrass Science
• /
• v.5 no.4
• /
• pp.236-240
• /
• 2016

Weeding measures is an important issue for cereal crop because weed is superior to crops in early growth stage and there are not many herbicides applicable to cereal crop. Since the concern about well-being and eco-environmental products are increasing, cultivation with polyvinyl chloride (PVC) is needed. Five treatments included as weed-free, control (not remove weed) and covering with polyvinyl chloride removed 20, 30, 40 DAS (day after seeding). At removing PVC, plant height did not differ depending on the growth stage. However, weed-free plot by weed competition were somewhat short. And, removing PVC accelerated the earlier by 2-3 day than weed-free plot. Soil moisture which removed from the control was higher than 20, 30, 40 DAS and weed-free, and it was kept low by evaporation. The chlorophyll meter of weed-free plot was 34.3, 30 DAS was 40.6 at heading time. Weed-free showed that increased in weed population $300.8g\;m^{-2}$. Weed control value of 20 DAS, 30 DAS, 40 DAS were 86.5%, 84.0% and 74.8%, respectively. In 30 DAS, yield of foxtail millet according to treatment of removing PVC were $355kg\;10a^{-1}$. At weed competition early in 30 DAS, the removing PVC was good for control. Thus, 30 DAS was judged to be suitable.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.8
• /
• pp.1097-1102
• /
• 2008

Paraoxonase-1 (PON1), like lipoprotein lipase (LPL), plays a key role in the metabolism and physiology of mammalian growth. The objectives of this study were to estimate the allele and genotype frequencies at the PON1/EcoRV and PON1/AluI loci in three genetic groups of beef cattle and to determine associations between these polymorphisms and growth and carcass traits. Genotyping was performed on 30 Angus, 32 Hereford and 26 Simmental. The association analysis was carried out using the GLM procedure of SAS 9.1 and the least squares means of the genotypes were compared by the Tukey's test. Animals with AG genotype at the PON1/EcoRV locus had higher weight at the time of entry into the fattening corrals ($329.97{\pm}6.08kg$) and close to the time of slaughter ($577.56{\pm}8.32kg$) and net meat weight ($275.89{\pm}4.05kg$) and fitted tenderness ($3.10{\pm}0.19kg$) (p<0.05). Animals with AA genotype at the PON1/AluI locus had higher weight at the time of entry ($333.37{\pm}8.93kg$) and slaughter ($576.82{\pm}13.18kg$) and net meat weight ($275.49{\pm}6.43kg$) and average daily gain ($0.68{\pm}0.02kg/d$) (p<0.05). The meat color score was also significantly higher (p<0.05). Between genotypes and breeds, there were significant differences observed except for TBW, REMG, MBS, REA and MCS. As a metabolism gene, genotypes of the SNPs of PON1 gene might be reflecting BFT directly, such as $A_eA_eG_aG_a$ genotype in this experiment.