• Title/Summary/Keyword: Erwinia sp.

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Biological Control of Pseudomonas sp. for Erwinia rhapontici Causing Vegetables Root Rot (채소연부병균 Erwinia rhapontic 에 대한 Pseudomonas sp. 의 생물학적 억제)

  • 김교창;김도영;도대홍
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.1
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    • pp.104-109
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    • 1994
  • For Selection of powerful antagonistic bacteria for biological control of soil borne Erwinia rhapontici causing rot of the vegetables and fruit, excellent straints (S43, S62) were selected from rhizopere in vegetables root rot suppressive soil. Selected strains were identified to be Pseudomonas sp. with Apl 20NE kit tests. Optimum culture condition for the maximum production of antagonistic substance was determined , when isolate was cultured in 523 synthetic broth media at pH 7.0 and 30 during 3 days. Antagonistic substance productivity of isolated Pseudomonas sp. (S43, S62) in the fertilizer soil were increased to about 40-50% compared to that in the non fertilizer soil.

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New Bacterial Soft Rot of Ornamental Foliage Plants by Erwinia carotovora subsp. carotovora in Korea (Erwinia carotovora subsp. carotovora에 의한 관엽식물의 새로운 세균성무름병)

  • 최재을;이은정
    • Research in Plant Disease
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    • v.6 no.1
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    • pp.19-22
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    • 2000
  • Ten samples were collected from soft rotted ornamental foliage plants, that were cultivated in the vinyl-houses in Taejeon, Yeoju, Seongnam, Kimhae and Cheju during 1998 to 1999. Studies on morphological, cultural, physiological and pathological characteristics indicated that the bacteria from Begonia heimalis, Saintpaulia sp. and Clivia miniata were Erwinia carotovora subsp. carotovora, E. carotovora subsp. carotovora the first description of bacteria which caused bacterial soft on Begonia heimalis, Saintpaulia sp., and Clivia miniata in Korea.

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Population Density Changes of Bacteria Causing Soybean Sprout Rot on Soybean Pods (콩 꼬투리에서 서식하는 세균 및 콩나물 부패균의 밀도 변화)

  • 이은정;한광섭;심명용;최재을
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.41-45
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    • 1999
  • Bacterial population densities on soybean pods from Chungnam province ranges 105~106 CFU/$\textrm{cm}^2$, whereas those of bacteria causing sprout rot ranged 0~103 CFU/$\textrm{cm}^2$. Erwinia chrysanthemi, Xanthomonas campestris pv. glycines, Staphylococcus sp., and Micrococcus sp. were identified as pathogenic bacteria causing soybean sprout rot. The population density of X. campestris pv. glycines was higher than those of other bacteria.

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Production of Pyrogallol from Gallic Acid by Erwinia sp. (Erwinia sp.에 의한 Gallic Acid로부터 Pyrogallol의 생산)

  • 박병화;황인균;방원기
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.665-671
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    • 1994
  • For the production of pyrogallot from gallic acid, about 100 strains of bacteria capable of assimilating gallic acid as a sole carbon and energy source were isolated from the soil. JH- 004 strain showing the highest activity of gallate decarboxy#lase was selected from them and identi- fied as Erwinia sp. The optimal conditions for the production of pyrogallol from gallic acid were examined. The resting cells of JH-004 cultured in a complex medium containing 0.2%(w/v) gallic acid were prepared after the treatment of the pellet with a freezing and thawing, and used as a enzyme source. The reaction mixtures for the maximal production of pyrogallol were shown to be 6 g/l of resting cells and 15 g/l of gallic acid in 25 mM potassium phosphate buffer. The optimal pH for the reaction was 5.0 and the optimal temperature was 35$\circ$C . Additionally, Triton X-100(0.01%, w/v) was found to be most effective for the production of pyrogallol. Under the above conditions, 10.27 g/l of pyrogallol was produced from 15 g/l of gallic acid after incubation of 35 hrs. This amount of pyrogallol corresponds to a 92.37% yields, based on gallic acid.

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Biohydrogen Production from Sugar Manufacturing Wastewater and Analysis of Microbial Diversity (제당폐수를 이용한 수소생산과 미생물의 군집해석)

  • Lee, Heesu;Lee, Tae-Jin
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.3
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    • pp.41-51
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    • 2012
  • Biohydrogen production and analysis of microbial community were attempted from the sugar manufacturing wastewater with anaerobic fermentation process. Addtion of nutrients ($N{\cdot}P$) into sugar manufacturing wastewater stimulates hydrogen production from 9.53 to $26.67m{\ell}$ $H_2/g$ COD. Butyric acid, acetic acid, lactic acid, and propionic acid were detected in the sample of the anaerobic fermentation process. Butyric acid/Acetic acid(B/A) ratio was increased 0.50 to 0.92 according to the nutrients addtion into the wastewater. Microbial community was analyzed as Clostridium sp. in the phylum of Firmicutes and Klebsiella sp., Erwinia sp., and enterobacter sp. of the class of $\gamma$-Proteobacteria. As the improvement of hydrogen production, Erwinia sp. was decreased and Klebsiella sp. was increased.

Bacterial Soft Rot of Dendrobium phalaenopsis and Phalaneopsis Species by Erwinia chrysanthemi

  • Lee, Dong-Hyun;Kim, Jung-Ho;Lee, Jae-Hong;Hur, Jae-Seoun;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.15 no.5
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    • pp.302-307
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    • 1999
  • Occurrence of soft rots was observed on Dendrobium phalaenopsis and Phalaenopsis sp. that were grown at the greenhouses in Sunchon and Kwangyang areas, Chonnam province of Korea in 1997 and 1998. Typical soft rot symptom appeared frequently on young plants of D. phalaenopsis and Phalaenopsis sp. Soft rot symptom usually appeared on old leaves of D. phalaenopsis, and extended into whole leaves, accompanying blighting of whole plants. Symptom began as a small water-soaked lesion on old leaves of Phalaenopsis sp., which enlarged rapidly on the leaves and eventually resulted in soft rots of whole plants. The causal organism isolated from the infected lesions was identified as Erwinia chrysanthemi based on its pathogenicity, physiological and biochemical characteristics, and the results of the BIOLOGTM program. The bacterial soft rot caused by e. chrysanthemi was firstly describe din D. phalaenopsis and Phalanopsis sp. in Korea.

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Activity of Some Intracellular Enzymes of Three Virulent Erwinia sp. in Presence of Some Heavy Metal Salts

  • Saleh, Youssry-E.;Naguib, Mohamed-I.;Shehata, Nabil-E.
    • Archives of Pharmacal Research
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    • v.13 no.4
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    • pp.298-305
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    • 1990
  • Based on equal number of cells, supplementation of 10$^{-6}$ M cadimium highly simulated the intracellular amylase. GCT, LDH as well as the glucose and erea content of E carotovora var, carotovaro cells. This was coupled with initiation of highly active GOT, CPK as well as accumulation of cholesterol in the cells. Lanthanum was less active and unable to initiate COT or CPK. Nickel was almost without effect though reduced LDH activity without initiating either enzyme or cholesterol production. Similar stiulations and/or initiations were observed, though to variable extents, when the same concentration of the three elements were supplied to E. carotovora var, citullis or E. toxica. The highest yield of amylase, GPT, GGT or glucose was obtained when E. carotovora var. carotovora was supplemented with Cd + Ni. The highest urea level was recorded in Erwinia carotovara var, cirullis, amended with Cd + La.

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Microbiological Characteristics of Heavy Metal Ion-Tolerant Microorganisms. (중금속 내성균주의 미생물학적 성질)

  • 유대식
    • Microbiology and Biotechnology Letters
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    • v.7 no.4
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    • pp.183-190
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    • 1979
  • Cadmium ion-tolerant microorganisms were isolated from the sludge and soil of a cadmium ion-polluted area, a zinc mineralized area, in Kyung Sang Pook Do, Korea. A strain, C-7, which showed tile highest tolerance to cadmium ion was selected by screening from 18 cadmium tolerant microorganisms. By the taxonomical characteristics of this strain, it was identified as a variant of Erwinia sp.. The strain grew in a medium cadmium ion up to a concentration of 2, 800 $\mu\textrm{g}$/ml and the maximum intercellular accumulation of Cd$^{2+}$ was measured to be 28.60 mg/g dried cells (57.2%) during incubation in medium containing 50 $\mu\textrm{g}$/ml under aerobic condition at 28$^{\circ}C$ for 24 hour.r.

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Antibacterial Activity of Onion Pathogens and Isolation of Bacillus ehimensis YJ-4 from the Rhizosphere of Healthy Onion Roots (건전 양파 근권으로부터 Bacillus ehimensis YJ-4의 분리 및 양파 병원균들에 대한 길항력 조사)

  • 주길재;이인구
    • Food Science and Preservation
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    • v.10 no.2
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    • pp.224-229
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    • 2003
  • This study was carried out to isolate of antagonistic bacterium to Allium cepa L. pathogens. A total of 250 strains were isolated from A. cepa L. roots. The isolates were screened for antagonism to A. cepa L. pathogens and the isolated strain No. YJ-4 was selected among these bacteria. It was identified as Bacillus ehimensis based on morphological and physiological characteristics according to the Bergey's mannual of systematic bacteriology, Sherlock system of Microbial ID Int and 165 rDNA sequences methods. Bacillus ehimensis YJ-4 showed broad spectrum of antibacterial and antifungal activities against plant pathogens as Alternaria porri, Botrytis cinerea, Erwinia carotovora subsp. carotovora Fusarium of oxysporium, penicillium sp., Pseudomonas sp., Rhizoctonia solani, Sclerotium cepivotum, Septoria sp., Stemphylium botryosum. Speially B. ehimensis YJ-4 showed high antifungal activity on growth against F. oxysporium, the causal agent of onion Fusarium wilt.

Molecular Cloning of Bacteriocin Gene and Biological Control of Plant Pathogen (Bacteriocin 생산 유전자의 Cloning 및 식물병원균에 대한 생물학적 억제)

  • 김교창;육창수;도대홍
    • Microbiology and Biotechnology Letters
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    • v.18 no.1
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    • pp.98-102
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    • 1990
  • A strain of Erwinia spp. was selected from the soil for the production of bacteriocin to the root rot plant pathogen. Bacteriocin producing gene was not located on plasmid but on chromosome. Genomic library of Erwinia spp. were made by using pLAFR 3 as a vector system for cloning of the gene. It was been cloned and expressed in E. coli DH 5 . Bacteriocin producing colony was composed of pLAFR 3 vector and 3.0 kb EcoRI fragment of Erwinia spp. ehromosomal DNA. The inserted fragment (3.0 kb) was possessed a EcoRI and BarnHI restriction sites.

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