• Title/Summary/Keyword: Euphorbia helioscopia

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Pollination study of Euphorbia helioscopia (Euphorbiaceae) (대극과 등대풀의 수분연구)

  • Kim, Deog-Il;Park, Ki-Ryong
    • Korean Journal of Plant Taxonomy
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    • v.44 no.4
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    • pp.281-287
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    • 2014
  • The present study was conducted to understand the growth pattern, pollinators and their frequency of visits of Euphorbia helioscopia population which is located at Tongyoung areas in Korea. The vegetative parts of the species grow early in March, and flowering begins in the middle of the month, and pollination was completed at the mid of April. As a typical form of early spring type, the fruits of E. helioscopia were matured between early in April and May, and the upper parts of plants were disappeared between May and June. During the blooming season about 11 insects species which belong to Diptera, Hymenoptera, Coleoptera and Heteroptera were the most common groups found on this plants. Among them the species of Diptera are the most dominant pollinators for E. helioscopia which is concordant to previous reports in Euphorbia species. Despite of significant numbers of insect visitors in Euphorbia species being reported, E. helioscopia attracts only 11 insect species in study areas, and the paucity of species probably associates with the insect fauna in Tongyoung areas or typical patterns of early spring bloomer of Euphorbias. We observed majority of Diptera species to visit in the morning, and the frequency of visit decreased in the afternoon. Thus, it suggests that the visiting activity of Diptera species is not associated with the temperature increase which was reported in Euphorbia species. However, the number of the cyathium during the blooming season is likely to be related to the frequency of visit of pollinators in E. helioscopia.

Antioxidative and Antimicrobial Activities of Euphorbia helioscopia Extracts (등대풀(Euphorbia helioscopia) 용매 추출물의 항산화 및 항균활성)

  • Kim, Ji-Young;Lee, Jung-A;Kim, Kil-Nam;Song, Gwan-Pil;Park, Soo-Yeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.9
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    • pp.1106-1112
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    • 2007
  • The solvent extracts of Euphorbia helioscopia, which were extracted by using several solvents with different polarities, were prepared for utility as natural preservatives. The E. helioscopia extract by 80% ethanol was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. In order to effectively screen for a natural preservatives agent, we first investigated the antioxidant activities such as DPPH radical scavenging capacity, superoxide radical scavenging capacity, and xanthine oxidase inhibitory activity of the E. helioscopia extracts. By the screening system, we found that ethylacetate fraction had the strongest antioxidant activity in a dose-dependent manner. The antimicrobial activities and cell growth inhibition were investigated for each strain with the different concentrations of E. helioscopia extracts. Antimicrobial activities were shown in ethylacetate fraction of E. helioscopia; however, ethanol, butanol and water fractions showed weak antimicrobial activity against the tested microorganisms. Among the five fractions, ethylacetate fraction showed the highest antimicrobial activities against microorganisms tested, such as Bacillus sublitis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella Enteritidis and Salmonella Typhimurium. The polyphenol content from ethanol, n-hexane, dichloromethane, ethylacetate, butanol, and water fractions were 207.46 mg/g, 45.45 mg/g, 138.23 mg/g, 678.02 mg/g, 278.91 mg/g, and 63.76 mg/g, respectively. There seems to be a close relationship between antioxidant activities, and antimicrobial activities and polyphenol content in natural plant. From these results, it is suggested that E. helioscopia could be used for the ethylacetate fraction and could be suitable for the development of a food preservative.

Inhibitory Effect of Hydrolysable Tannins Isolated from the Euphorbia helioscopia on Mushroom Tyrosinase Activity in vitro (대극과식물 등대풀로부터 분리한 가수분해형 탄닌의 tyrosinase 활성 억제효과)

  • 김진준;이주상;김소영;김정아;정시련
    • YAKHAK HOEJI
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    • v.45 no.2
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    • pp.214-219
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    • 2001
  • Nineteen hydrolysable tannins isolated from the Euphorbia helioscopia (Euphorbiaceae) were tested for the inhibitory effect on mushroom tyrosinase activity in vitro. Inhibitory effect of gallotannin group exhibited more potent than that of phenolcarboxylic acid and ellagitannin groups against the enzyme activity. The inhibitory activity by pentagalloyl glucose on mushroom tyrosinase was more potent ($IC_{50}$/, 4.9 $\mu$M) than that of kojic acid ($IC_{50}$/, 8.7 $\mu$M).

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Protective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death (등대(燈臺)풀 유래 Furosin의 glutamate에 의한 HT22 세포 사멸 억제 효과)

  • Baek, Ji Yun;Song, Ji Hoon;Choi, Sung Youl
    • The Journal of Korean Medicine
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    • v.39 no.1
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    • pp.35-43
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    • 2018
  • Objectives: In the brain, glutamate is the most important excitable neurotransmitter in physiological and pathological conditions. However, the high level of glutamate induces neuronal cell death due to exitotoxicity and oxidative stress. The present study investigated to evaluate a possible neuroprotective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death. Methods: Furosin was isolated from methanol extract of Euphorbia helioscopia and examined whether it protects glutamate-induced neuronal cell death. The cell viability was determined using Ez-Cytox assay. Anti-oxidative effect of furosin was determined by DPPH scavenging activities, and the levels of intracellular reactive oxygen species (ROS) were determined by the fluorescent intensity after staining the cells with $H_2DCFDA$. To evaluate apoptotic cell death, we performed nuclear staining and image-based cytometeric analysis. Results: The cell viability was significantly increased by treatement with furosin compared with the treatment with glutamate. Furosin showed a strong DPPH radical scavenging activity ($EC50=1.83{\mu}M$) and prevented the accumulation of intra cellular ROS. Finally, the presence of 50 and $100{\mu}M$ furosin significantly the percentage of apoptotic cells compared with glutamate treatment. Conclusion: The present study found that furosin is a potent neuroprotectant against glutamate-induced oxidative stress through inhibition of apoptotic cell death induced by glutamate. Therefore, the present study suggests that furosin as a bioactive compound of E. helioscopia can be a useful source to develop a drug for the treatment of neurodegenerative diseases and acute brain injuries.

New Compounds from Euphorbia helioscopia and Absolute Configuration Determination by Computational Methods

  • He, Jiang-Bo;Zhu, Hua-Jie;Luo, Gui-Fen;Liu, Guang-Ming;Chen, Hao;Li, Yan;Chen, Shaopeng;Lu, Xin;Zhou, Guochun;Cheng, Yong-Xian
    • Bulletin of the Korean Chemical Society
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    • v.31 no.8
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    • pp.2211-2214
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    • 2010
  • The whole plant of Euphorbia helioscopia is an important traditional Chinese medicine. Fom its BuOH soluble extract, one new lactam (1), three new terpenoids (2-4) including a new naturally occurring compound, and three known compounds were isolated. Their structures were identified by spectroscopic evidences. In particular, the absolute configurations of side chain of compounds 1 and 2 were determined using computational methods.

Anti-Allergic and Anti-Asthmatic Activity of Helioscopinin-A, a Polyphenol Compound, Isolated from Euphorbia helioscopia

  • Park, Kwan-Ha;Koh, Dong-Soo;Lee, Seung-Ho;Jung, Ill-Min;Kim, Kyung-Hyun;Lee, Chul-Hoon;Kim, Kye-Hoon;Lim, Yoong-Ho
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.138-142
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    • 2001
  • During the course of searching for anti-allergic substances from unexplored plant sources, an inhibitor of leukotriene $D_4$-induced tracheal contraction was isolated from Euphoribia helioscopia. This isolated polyphenol compound, known as helioscopinin-A, showed a certain inhibitory activity on capillary permeability in passive cutaneous anaphylaxis responses of rats and also on antigen-induced bronchial constriction in an experimental asthma model of guinea pigs. The compound at a high concentration weakly inhibited histamine release from isolated mast cells of rats. It is suggested that this compound is an anti-allergic and anti-asthmatic which exerts its activity through antagonism on leukotrene $D_4$-induced responses. A partial inhibition of allergic mediator relase may also bee involved.

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Studies on the Effect of Corilagin Isolated from Euphorbia helioscopia on Collagen-Induced Arthritis (I);Analysis of Fluorescence Flow Cytometry from Collagen II Induced Arthritis Mice Model (택칠에서 분리한 Corilagin이 Collagen 유발 관절염에 미치는 영향 (I);Corilagin을 투여한 류마티스 관절염 유발 생쥐의 형광유세포 분석)

  • Shin, Sam-Kee;Jang, Jun-Pok;Doh, Eun-Soo
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.329-335
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    • 2008
  • This study was intended to research into the possibility of corilagin (CRN) isolated from Euphorbia helioscopia as rheumatoid arthritis drug. For the study, CRN was medicated to the abdominal cavity of collagen induced arthritis (CIA) mice that was a animal model for rheumatoid arthritis and its effects on incidence and arthritis index (AI) were studied. The results were as folllows; Medicating corilagin that were isolated from Euphorbia heliscopia to CIA mice resulted in inhibiting an incidence of arthritis and reducing in arthritis index. It was found that inflammatory cells were remarkably decreased and joint cavity was well secured. Also it was displayed that the formation of pannus was not observed and a cartilage was preserved well by cell histological research. Performing fluorescence flow cytometry of CIA mice resulted in reducing in inflammatory cells infiltrated each tissue.

Studies on the Effect of Corilagin Isolated from Euphorbia helioscopia on Collagen-Induced Arthritis (II);CytokinesAnalysis of Collagen II Induced Arthritis Mice Model (택칠에서 분리한 Corilagin이 Collagen 유발 관절염에 미치는 영향 (II);Corilagin을 투여한 류마티스 관절염 유발 생쥐의 사이토카인 분석)

  • Shin, Sam-Kee;Chang, Jun-Pok;Doh, Eun-Soo
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.336-340
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    • 2008
  • Corilagin (CRN) isolated from Euphorbia helioscopia as rheumatoid arthritis drug. CRN was medicated to the abdominal cavity of collagen induced arthritis (CIA) mice that was an animal model for rheumatoid arthritis and its effects on incidence and arthritis index were studied. The results were as folllows; It was exhibited that medicating corilagin inhibited the infiltration of activated T lymphocytes into an inflammatory joint. The production of IgG and IgM that were RF (rheumatoid factor) and inflammatory cytokine, IL-6 and $TNF-{\alpha}$were reduced. After measuring $IFN-{\gamma}$and IL-4, it was found that it was shifted into Th2 immune response as increasing in IL-4. After liver function test, studies on liver poisoning of AST/ALT should be continued.

Cytotaxonomic study of Korean Euphorbia L. (Euphorbiaceae) (한국산 대극속(Euphorbia L., Euphorbiaceae)의 세포분류학적 연구)

  • Chung, Gyu Young;Oh, Byoung-Un;Park, Ki-Ryong;Kim, Joo-Hwan;Kim, Mi Suk;Nam, Gi-Heum;Jang, Chang-Gee
    • Korean Journal of Plant Taxonomy
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    • v.33 no.3
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    • pp.279-293
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    • 2003
  • Somatic chromosomes about 13 taxa of Korean Euphorbia L. was investigated to estimate its taxonomic significance. Somatic chromosome numbers of treated taxa were 2n= 12, 20, 22, 28, 40, 42, 56, therefore basic chromosome numbers of those were x=6, 7, 10, 11. The chromosome numbers of E. pallasii Turcz. (2n=20), E. hylonoma Hand.-Mazz (2n=20.), E. fauriei H. L$\acute{e}$v. & Vaniot ex H. L$\acute{e}$v (2n=28) and E. jolkini Boiss. (2n=28) were determined for the first time in this study. The chromosome numbers of four taxa were same as previous ones; E. sieboldiana Moor. & Decne. (2n=20), E. ebracteolata Hayata (2n=20), E. humifusa Willd. ex Schlecht. (2n=22). But those of six taxa were different; E. esula L (2n= 16, 20, 60, 64 vs 2n=20), E. helioscopia L. (2n=12, 42 vs 2n=42), E. lucorum Rupr. (2n=28, 40 vs 2n=56), E. pekinensis Rupr. in Maxim. (2n=24 vs 2n=28, 56), E. maculata L. (2n=28, 42 vs 2n=12), E. supina Raf. (n=7 vs 2n=40). E. ebracteolata, E. pallasii and E. hylonoma were distingushcd from the other taxa by the chromosome numbers, size and satellites, E. maculata, E. humifusa, E. supina had the different basic and somatic chromosome numbers in spite of the similar morphological. anatomical and palynological chracters. The chromosomal character of Korean Euphorbia was supported the Ma and Hu's systems, and as above results, it was found to be a good character in delimiting above sections and estimating relationships for some species.

Assessment of β-Lactamase Inhibitor Potential of Medicinal Plant Extracts against Antibiotic-resistant Staphylococcus aureus

  • Dawan, Jirapat;Ahn, Juhee
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.578-585
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    • 2020
  • This study was designed to assess the possibility of using medicinal plant extracts as β-lactamase inhibitors to control antibiotic-resistant Staphylococcus aureus. The susceptibilities of S. aureus ATCC 15564 (SAWT), ciprofloxacininduced S. aureus ATCC 15564 (SACIP), oxacillin-induced S. aureus ATCC 15564 (SAOXA), and clinically-isolated S. aureus CCARM 3008 (SACLI) to ampicillin were determined in the absence and presence of medicinal plant extracts, including Cleyera japonica (CJ), Carpinus laxiflora (CL), Euphorbia helioscopia (EH), Euscaphis japonica (EJ), Oenothera erythrosepala (OE), and Rosa multiflora (RM). The phenotypic change in the clear inhibition zones around ampicillin disc was observed for SAWT, SACIP, and SAOXA, indicating the production of ampicillinase. Compared to the controls, the MICs of ampicillin against SAWT, SACIP, and SAOXA were decreased from 4 to 0.5 ㎍/mL in the presence of CL, 16 to 4 ㎍/mL in the presence of RM, and 32 to 2 ㎍/mL in the presence of CL, EH, and RM, respectively. The medicinal plant extracts, OE, EJ, and CL, effectively inhibited the β-lactamase activities of SAWT (78%), SACIP (57%), and SAOXA (76%) when compared to the control. This results suggest that the medicinal plant extracts can be used as BLIs to control the antibiotic-resistant S. aureus.