• Title/Summary/Keyword: Ex-situ test

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Ex situ Coloration of Laccase-Entrapped Bacterial Cellulose with Natural Phenolic Dyes

  • Kim, Hyunjin;Song, Ji Eun;Kim, Hye Rim
    • Journal of the Korean Society of Clothing and Textiles
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    • v.45 no.5
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    • pp.866-880
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    • 2021
  • This study aimed to ex situ colorize laccase-entrapped bacterial cellulose (BC) with natural phenolic dyes, namely,madder, turmeric, and cochineal, and to determine the effect of laccase entrapment on the dyeability of BC using color strength (K/S) analysis. Results showed that laccase entrapment improved the dyeability of BC and that pre-entrapment was the most effective method, compared with meta-entrapment and post-entrapment methods. In addition, surface characterizations confirmed the successful entrapment of laccase inside the BC nanostructure and retention of the cellulosic and crystalline structures of BC. The washing durability test confirmed that the K/S value of BC had improved after laccase entrapment. Furthermore, laccase-entrapped BC colorized with cochineal dye had the highest washing durability due to the high molecular weight of cochineal dyerelative to the other dyes. This study suggests a novel method for enhancing the dyeability and washing durability of BC colorized ex situ with natural phenolic dyes by laccase entrapment.

Prussian blue immobilization on various filter materials through Layer-by-Layer Assembly for effective cesium adsorption

  • Wi, Hyobin;Kim, Hyowon;Kang, Sung-Won;Hwang, Yuhoon
    • Membrane and Water Treatment
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    • v.10 no.3
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    • pp.245-250
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    • 2019
  • Prussian blue (PB) is well known for its excellent $Cs^+$ ions adsorption capacity. Due to the high dispersibility of PB in aqueous phase, composite materials imbedding PB in supporting materials have been introduced as a solution. However, building PB particles inside porous supporting materials is still difficult, as PB particles are not fully formed and elute out to water. In this study, we suggest layer-by-layer (LBL) assembly to provide better immobilization of PB on supporting materials of poly vinyl alcohol sponge (PVA) and cellulose filter (CF). Three different PB attachment methods, ex-situ/in-situ/LBL assembly, were evaluated using PB leaching test as well as $Cs^+$ adsorption test. Changes of surface functionality and morphology during PB composite preparation protocols were monitored through Fourier transform infrared spectroscopy and scanning electron microscopy. The results indicate that LBL assembly led to better PB attachment on supporting materials, bringing less eluting PB particles in aqueous phase compared to other synthesis methodologies, such as ex-situ and in-situ synthesis. By enhancing the stability of the adsorbent, adsorption capacity of PVA-PB with LBL improved nine times and that of CF-PB improved over 20 times. Therefore, the results suggest that LBL assembly offers a better orientation for growing PB particles on porous supporting materials.

유류오염토양 복원을 위한 설계인자 선정에 관한 연구

  • 조장환;전권호;서창일;박정구
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.293-296
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    • 2004
  • The objective of this study was to decide the designing factor for remediaton of the contaminated site. The soil and ground-water samples were analyzed and hydro- geological characteristics was assayed for the survey of pollution level. Also air-permeability test and MPN(most probable number) test were conducted for selecting the designing factor. The contaminants were mainly found in north-west part of the site and were expected to move toward the south. Ex-situ technology was expected more useful than in-situ one with the results of air-permeability test saying that air permeability was relatively low. Additional microbes were expected for remediation efficiency because residual microbes were loosely populated. The choosing of the designing factor was requisite for remediation of contaminated site.

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Electrochemical Characteristics of $V_2O_5$ based All Solid State Thin Film Microbattery by Ex-situ Sputtering Method (Ex-situ 스퍼터링법에 의한 $V_2O_5$ 전 고상 박막전지의 전기화학적 특성)

  • Lim Y.C.;Nam S.C.;Jeon E.J.;Yoon Y.S.;Cho W.I.;Cho B.W.;Chun H.S.;Yun K.S.
    • Journal of the Korean Electrochemical Society
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    • v.3 no.1
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    • pp.44-48
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    • 2000
  • Amorphous $V_2O_5$ cathode thin films were prepared by DC-magnetron sputtering at room temperature and the thin film rechargeable lithium batteries were fabricated with the configuration of $V_2O_5/LIPON/Li$ using sequential ex-situ thin film deposition techniques. The electrochemical characteristics of $V_2O_5$ cathode materials Prepared at 80/20 of $Ar/O_2$ ratio showed high capacity and cycling behaviors by half cell test. LIPON solid electrolytes films were prepared by RF-magnetron sputtering using the self-made $Li_3PO_4$ target in pure $N_2$ atmosphere, and it was very stable for lithium contact in the range of 1.2-4.0 V vs. Li. Metallic lithium were deposited on LIPON electrolyte by thermal evaporation methode in dry room. Vanadium oxide based full cell system showed the initial discharge capacity of $150{\mu}A/cm^2{\mu}m$ in the range of $1.2\~3.5V$.

Isolation of an Indigenous Imidacloprid-Degrading Bacterium and Imidacloprid Bioremediation Under Simulated In Situ and Ex Situ Conditions

  • Hu, Guiping;Zhao, Yan;Liu, Bo;Song, Fengqing;You, Minsheng
    • Journal of Microbiology and Biotechnology
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    • v.23 no.11
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    • pp.1617-1626
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    • 2013
  • The Bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. The dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, Rhizobium sp., Sinorhizobium, Ochrobactrum sp., Alcaligenes, Bacillus sp., Bacterium, Klebsiella sp., and Ensifer adhaerens. The bacterial community structure was altered extensively and its complexity reduced during the enrichment process, and four culturable bacteria, Ochrobactrum sp., Rhizobium sp., Geobacillus stearothermophilus, and Alcaligenes faecalis, remained in the final enrichment. Only one indigenous strain, BCL-1, with imidacloprid-degrading potential, was isolated from the sixth enrichment culture. This isolate was a gram-negative rod-shaped bacterium and identified as the genus Ochrobactrum based on its morphological, physiological, and biochemical properties and its 16S rRNA gene sequence. The degradation test showed that approximately 67.67% of the imidacloprid (50 mg/l) was degraded within 48 h by strain BCL-1. The optimum conditions for degradation were a pH of 8 and $30^{\circ}C$. The simulation of imidacloprid bioremediation by strain BCL-1 in soil demonstrated that the best performance in situ (tea soil) resulted in the degradation of 92.44% of the imidacloprid (100 mg/g) within 20 days, which was better than those observed in the ex situ simulations that were 64.66% (cabbage soil), 41.15% (potato soil), and 54.15% (tomato soil).

Strategy for Bio-Diversity and Genetic Conservation of Forest Resources in Korea (생물종(生物種) 다양성(多樣性) 및 삼림유전자원(森林遺傳資源) 보존(保存) 전략(戰略))

  • Park, Young Goo
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.191-204
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    • 1994
  • Due to its topographic complexities and various climatical condition, Korea exhibits diverse forest types. Dominant tree species in this zone are Quercus spp., Betula spp., Zelkova spp., Fraxinus spp., Pinus densiflora, Pinus koraiensis, and Pinus thunbergii ete. Genetic conservation in forest species in Korea there are three ways ; one is in situ, other is ex situ and third is in-facility conservation. In situ conservation include that are the present status of conservation of rare and endangered flora and ecosystem, the reserved forest, the national and provincial park, and the gene pool of natural forests. Ex situ conservation means to be established the new forest from in situ forest stands, progeny and provenance test populations, seed orchard and clone banks, and gene conservation in-facility. As a tool for low temperature storage, several aspects on in vitro system were studied ; (1) establishment of in vitro cultures from juvenile and/or rejuvenated tissues, (2) induction of multiple shoots from the individual micropropagules, (3) elongation of the proliferated shoots. Studies on cold storage for short-and long-term maintenance of in vitro cultures under $4^{\circ}C$ in the refrigerator were conducted. For the cryopreservation at $-196^{\circ}C$, various factors affecting survivability of the plant materials are being examined. The necessity of gene conservation of forest trees is enlarged not only to increase the adaptability for various environments but also to gain the breeding materials in the future. For effective gene conservation of forest trees, I would like to suggest followings ; 1. Forest stands reserved for other than the gene conservation purposes such as national parks should be investigated by botanical and gene-ecological studies for selecting bio-diversity and gene conservation stands. 2. Reserved forest for gene pool should be extented both economically important tree spp. and non-economical species. 3. Reserved forest for progeny test and clone bank should be systematically investigated for the use of Ex situ forest gene conservation. 4. We have to find out a new methodology of genetic analysis determining the proper and effective size of subpopulation for in situ gene conservation. 5. We should develop a new tree breeding systems for successful gene conservation and utilization of the genetic resources. 6. New method of in-facility gene conservation using advanced genetic engineering should be developed to save time and economic resources. 7. For the conservation of species with short-life span of seed or shortage of knowledge of seed physiology, tissue culture techniques will be played a great role for gene conservation of those species. 8. It is are very useful conservation not only of genes but of genotypes which were selected already by breeding program. 9. Institutional and administrative arrangements including legistlation must be necessarily taken for gene conservation of forest trees. 10. It is national problems for conservation of forest resources which have been rapidly destroyed because of degenerating environmental condition and of inexperienced management system of bio-diversity and gene conservation. 11. In order to international cooperation for exchanging data of bio-diversity and gene conservation, we should connect to international net works as soon as possible.

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A Study on the Strength Characteristics of Model Ice for Warm-up Time during Model Ice Preparation (모형빙 생성 시 승온 시간에 따른 모형빙의 강도 특성 연구)

  • Jeong, Seong-Yeob;Ha, Jung-Seok
    • Journal of the Society of Naval Architects of Korea
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    • v.57 no.1
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    • pp.15-22
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    • 2020
  • Understanding the strength characteristics of model ice is an important issue for model testing in an ice model basin to estimate the ship performance in ice. In particular, the mechanical properties of the model ice including elastic modulus, flexural strength and compressive strength are key consideration factors. In order to understand the characteristics of the model ice during warm-up phase at KRISO's ice model basin, the strength properties are tested in this study. The infinite plate-bending method, in-situ cantilever beam test and ex-situ uniaxial compressive test are conducted to determine the strength properties of model ice. The strength characteristics of the model ice are then analyzed in terms of the warm-up phase and seasonality. These results could be valuable to quality control of the model ice characteristics in KRISO's ice model basin and to better understand the variations in strength properties during the ice model tests.

Impact of gamma radiation on 8051 microcontroller performance

  • Charu Sharma;Puspalata Rajesh;R.P. Behera;S. Amirthapandian
    • Nuclear Engineering and Technology
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    • v.54 no.12
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    • pp.4422-4430
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    • 2022
  • Studying the effects of gamma radiation on the instrumentation and control (I&C) system of a nuclear power plant is critical to the successful and reliable operation of the plant. In the accidental scenario, the adverse environment of ionizing radiation affects the performance of the I&C system and it leads to inaccurate and incomprehensible results. This paper reports the effects of gamma radiation on the AT89C51RD2, a commercial-off-the-shelf 8-bit high-performance flash microcontroller. The microcontroller, selected for the device under test for this study is used in the remote terminal unit for a nuclear power plant. The custom circuits were made to test the microcontroller under different gamma doses using a 60Co gamma source in both ex-situ and in-situ modes. The device was exposed to a maximum dose of 1.5 kGy. Under this hostile environment, the performance of the microcontroller was studied in terms of device current and voltage changes. It was observed that the microcontroller device can operate up to a total absorbed dose of approximately 0.6 kGy without any failure or degradation in its performance.

Efficient Screening of Surfactant for Soil Washing (토양세척을 위한 계면활성제의 효과적 선정)

  • 신현무;이상화
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1999.10a
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    • pp.7-11
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    • 1999
  • Soil washing process being operated in ex-situ mode using surfactants could be appropriate one of the most effective one for remediation. The choice of surfactants has been considered most significantly to accomplish tile reduction of expenditure and the increase of efficiency. This study was carried out screening test and solubility, washing experiment, and surfactant sorption experiments for 18 kinds of surfactant obtained. Results from each surfactant's PSR obtained by the slope indicated that nonionic surfactants have much higher solubility for HOCs than anion surfactants for that. The washing experiment to find out a removal efficiency of each surfactant's TPH, LE1017 and LE1019 showed high removal efficiency. Through on the result of estimating the extent of adsorption of surfactants for soils, nonion surfactants showed higher adsorption to soils than anion surfactants.

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A Study on Life Style and Daily Clothing Selection Motives of Men (남성의 라이프 스타일과 매일의 의복선택동기에 관한 연구)

  • 한화숙
    • Journal of the Korean Society of Costume
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    • v.28
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    • pp.137-150
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    • 1996
  • The objectives of this study were to classify daily clothing selection motives and to group men into life style types and to examine the differences in daily clothing selection motives according to the life style types. Questionnaire comprised of three sections: 36 Likert type items of life style measure; 29 Likert type items of daily clothing selection motives measure: and 4 demographic variables. Samples were 267 salary men in gheir 20's to 30's in Seoul Korea. The data were analyzed using factor analy-sis cluster analysis one-way ANOVA Dun-can's multiple range test and t-test. 1. Five factors of life style were derived by factor analysis ; F. 1 'appearance oriented' f. 2. 'positive activity' F. 3 'economy oriented': F.4 'active-leisure';F. 5 'contemporary' Four types of life style of men were defined by cluster analysis of the five factors: T. 1'posi-tive activity'; T. 2.'economy interest'; T. 3'contemporary' : T 2'economy interest' T.3'contemporary' ; T. 4 'appearance interest' 2. Four factors of daily clothing selection motives were derived by factor analysis: F.1 'clothing harmony'; F.2 'clothing utility'; F.3 'psychological influence'; F.4 'exogenous situ-ation adaptation 3. There were significant differences in daily clothing selection motives according to the life style types. The types of positive activity economy interest and contemporary had 'clothing utility' motive more than appearance interest type. Economy interest type had 'hpsychological influence' motive more than other three types. Economy interest type and appearance interest type had 'exogenous situ-ation adaptation' motive more than other two types 4. 20's and unmarried men had'psychological influence' motive more than 30's and married. Upper class had 'clothing harmony' and 'ex-ogeneous situation adaptation' motives more than middle and low. Men with less education had ' clothing utility' and 'exogenous situation adaptation' motives more than did more edu-cation.

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