• Journal of Life Science
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• v.26 no.9
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• pp.1027-1032
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• 2016

Antigen is substance causing disease derived from pathogen. Living organism has the immune system in terms of defense mechanism against antigen. Antigen is processed through several pathways such as phagocytosis, antibody action, complement activation, and cytotoxins by NK or cytotoxic T lymphocyte via MHC molecule. Lymph node (LN) is comprised of the complicated 3 dimensional network and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in T zone for interaction with T cells. FRC produces the extra cellular matrix (ECM) into LN for ECM reorganization against pathogen infections and secretes homing chemokines. However, it has not so much been known about the involvement of the antigen process of FRC. The present report is for the function of FRC on antigen process. For this, FRC was positioned with several infected situations such as co-culture with macrophage, T cell, lipopolysaccharide (LPS) and TNFα stimulation. When co-culture between FRC with macrophage and T cells was performed, morphological change of FRC was observed and empty space between FRCs was made by morphological change. The matrix metallo-proteinase (MMP) activity was up-regulated by Y27632 and T cells onto FRC. Furthermore, inflammatory cytokine, TNFα regulated the expression of adhesion molecules and MHC I antigen transporter in FRC by gene chip assay. NO production was elevated by FRC monolayer co-cultured with macrophage stimulated by LPS. GFP antigen was up-taken by macrophage co-cultured with FRC. Collectively, it suggests that FRC assists of the facilitation of antigen process and LN stroma is implicated into antigen process pathway.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.7
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• pp.1340-1345
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• 2010

FRC(Frame Rate Control) has been applied to the monitor and TV product as part of cost-saving in many flat panel display manufacturers. As FRC can represent the same number of gray scale level with bits of a smaller amount than bits of the input image data, it is widely used. However, FRC causes visual artifacts by using repeatedly pre-designed the FRC unit size of block pattern in display devices. Therefore, this paper analyzes the cause of the visual artifacts. And in order to improve them, it proposed the pattern arrangement of FRC unit blocks through frame rolling method as analytic solution for the first time. So, we could embody causes of FRC noise. Using the proposed structure, more robust pattern to FRC noise will be designed.

• Journal of Life Science
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• v.23 no.10
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• pp.1199-1208
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• 2013

Fibroblastic reticular cells (FRCs) form the structural backbone of the T zone provide a guidance path for immigrating T cells in the lymph node (LN). FRCs may contribute directly to developing T-cell biology in the LN and allow analyses of fundamental aspects of FRC biology related to T cells. FRCs inhibited T-cell apoptosis, and FRC culture supernatants strongly induced the expression of Bcl-xL in T cells against doxorubicin. Coculture of FRC and T cells resulted in rearrangements of the actin cytoskeleton, as well as global changes in the morphology of the FRCs. In addition, when cocultured, the T cells adhered to the FRC monolayer, and the membrane intercellular adhesion molecule (ICAM)-1 was slightly increased by day-dependent manner. In contrast, the expression of soluble ICAM-1 was dramatically increased in a day-dependent manner. Several chemokines, such as CCL5, CXCL1, CXCL5, CXCL16, CCL8, CXCL13, and ICAM-1, and MMPs were expressed in FRCs sensed by tumor necrosis factor (TNF) families. Nuclear factor kappa B ($NF{\kappa}B$)-RelA of the $NF{\kappa}B$ canonical pathway was translocated into FRC nuclear by $TNF{\alpha}$. In contrast, p52 proteolyzed from p100, a counterpart of RelB of the noncanonical $NF{\kappa}B$ pathway, accumulated in the peripheral FRC nucleus by agonistic anti-$LT{\beta}R$ antibody. In summary, we propose a model in which FRCs engage in bidirectional crosstalk to increase the efficiency of T-cell biology. This cooperative feedback loop may help to maintain tissue integrity and function during immune responses.

• Journal of Life Science
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• v.26 no.7
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• pp.789-795
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• 2016

A lymph node (LN) is one of the secondary lymphoid organs. An LN consists of a complicated 3 dimensional frame structure and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in the T zone for interaction with T cells. FRC secrete homing chemokines such as CCL19 and CCL21. Moreover, FRC play a pivotal role in the production of extracellular matrix (ECM) into LN for ECM reorganization against pathogen infections. However, not much is known about the involvement of the immune reaction of FRC. The present report is for the characterization of FRC on immune response. For this, FRC were positioned in several infected situations such as co-culture with macrophage, lipopolysaccharide (LPS), and TNFα stimulation. When a co-culture between FRC and macrophage was performed, a morphological change in FRC was observed, and empty space between FRCs was created by this change. The soluble ICAM-1 protein level was up-regulated by co-culturing with Raw264.7 and the treatment of the ROCK inhibitor Y27632. The activity of matrix metalloproteinase (MMP) was up-regulated by LPS onto FRC. Furthermore, the inflammatory cytokine TNFα regulated the expression of ECM in FRC by a gene chip assay. Collectively, it suggests that FRC are involved in immune reactions.

• Journal of Life Science
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• v.23 no.11
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• pp.1409-1414
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• 2013

Lymph node (LN) is the sites where mature lymphocytes become stimulated to respond to invading pathogens in the body. Lymphocytes screen the surfaces of pathogen-carrying antigen-presenting cells for cognate antigens, while moving along stromal structural back bone. Fibroblastic reticular cells (FRC) is stromal cell forming the 3 dimensional structure networks of the T cell rich zones in LN, and provide a guidance path for immigrating T lymphocytes. In these cooperative environments, the cell to cell bidirectional interactions between FRC and T cells in LN are therefore essential to the normal functioning of these tissues. Not only do FRCs physically construct LN architecture but they are essential for regulating T cell biology within these domains. FRC interact closely with T lymphocytes, is providing scaffolds, secreting soluble factors including cytokine in which FRCs influence T cell immune response. More recently, FRC have been found to induce peripheral T cell tolerance and regulate the extent to which newly activated T cells proliferate within LN. Thus, FRC-T cell crosstalk has important consequences for regulating immune cell function within LN. In addition, FRC have profound effects on innate immune response by secreting anti-microbial peptides and complement, etc in the inflammatory milieu. In summary, we propose a model in which FRC engage in a bidirectional touch to increase the T cell biological efficiency between FRC and T cells. This collaborative feedback loop may help to maintain tissue function during inflammation response.

• Advances in concrete construction
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• v.11 no.2
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• pp.111-126
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• 2021

Concrete cracking due to brittle tension strength significantly prevents fully utilization of the materials for "flexural-shear failure" type shear walls. Theoretical and experimental studies applying fiber reinforced concrete (FRC) have achieved fruitful results in improving the seismic performance of "flexural-shear failure" reinforced concrete shear walls. To come to an understanding of an optimal design strategy and find common performance prediction method for design methodology in terms to FRC shear walls, seismic performance on shear walls with PVA and steel FRC at edge columns and plastic region are compared in this study. The seismic behavior including damage mode, lateral bearing capacity, deformation capacity, and energy dissipation capacity are analyzed on different fiber reinforcing strategies. The experimental comparison realized that the lateral strength and deformation capacity are significantly improved for the shear walls with PVA and steel FRC in the plastic region and PVA FRC in the edge columns; PVA FRC improves both in tensile crack prevention and shear tolerance while steel FRC shows enhancement mainly in shear resistance. Moreover, the tensile strength of the FRC are suggested to be considered, and the steel bars in the tension edge reaches the ultimate strength for the confinement of the FRC in the yield and maximum lateral bearing capacity prediction comparing with the model specified in provisions.

• The korean journal of orthodontics
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• v.35 no.4 s.111
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• pp.302-311
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• 2005

This study was performed to compare the fatigue limit of stainless steel wires and Fiber-reinforced composites (FRC) under conditions of permitting physiologic tooth movement. and to evaluate the clinical value of FRCs which was used to reinforce the anchorage unit. The stainless steel wire groups were divided into round and rectangular wire groups. The FRC groups were divided into uni-directional and woven groups, with resin coating and without resin coating in the Proximal area After the number of cycles to failure of each of the 6 groups were measured within the $5{\times}10^5\;cycle$ fatigue limit simulating the orthodontic treatment period. the fatigue limit of each group was compared with each other The findings of this study were as follows. In stainless steel wires, the fatigue limit of rectangular wires were higher than that of round wires. But there was no statistically significant difference (p>0.05). In FRCs with resin coating and without resin coating in the interproximal area, the fatigue limit of uni-directional type was higher than that of the woven type (p<0.05). In uni-directional and woven type FRCs, the fatigue limit of FRC with resin coating in the interproximal area was higher thar that of FRC without resin coating (P<0.05) As the FRCs and stainless steel wires did not fracture until the $5{\times}10^5\;cycle$ fatigue limit which clinically is useful. it is sufficient to use FRC and stainless steel wire for reinforcing anchorage. When esthetics is important and the attachment of additional devices are necessary. it seems sufficient to use FRC as anchorage reinforcement.

• Journal of the Korea institute for structural maintenance and inspection
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• v.6 no.3
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• pp.119-128
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• 2002

This study discusses the issues related to the accuracy of deflection measurement and unstable energy in the testing of FRC. Some deflection methods may include large extraneous deformations. A faulty load-deflection curve will be obtained if an unstable deflection measuring system is used, and inaccurate toughness evaluation can result from this faulty curve. Some load-deflection curve of FRC may be attributed to unstable region of the load-deflection curve. If the unstable region is not correctly evaluated toughness indices from the curve would inappropriately represent true indices. In this paper, the discussion will focus on the effects of the deflection measuring system both on the measurement of the load-deflection response of FRC and the evaluation of FRC toughness and the effects of the unstable region and the management method of unstable region on toughness evaluation of FRC. It is observed that ASTM toughness indices which is based on measured deflection at first cracking is influenced significantly by extraneous deformation of deflection measurement. Extraneous deformation in deflection measurement, however result in negligible errors in toughness evaluation if JSCE and JCI definitions are used.

• Fibers and Polymers
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• v.5 no.3
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• pp.187-197
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• 2004

The compressive and flexural properties of hemp fiber reinforced concretes (FRC) were examined in this paper. Natural hemp fiber was mixed using dry and wet mixing methods to fabricate the FRC. Mechanical properties of the FRC were investigated. The main factors affecting compressive and flexural properties of the FRC materials were evaluated with an orthogonal test design. Fiber content by weight has the largest effect. The method for casting hemp FRC has been optimised. Under the optimum conditions, compressive strength increased by 4 %, flexural strength increased by 9 %, flexural toughness increased by 144 %, and flexural toughness index increased by 214 %.

• Journal of Life Science
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• v.20 no.5
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• pp.670-675
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• 2010

Autoimmune regulator gene (Aire) is expressed in the thymus and controls the expression of peripheral self-antigens, known as promiscuous genes. Aire and promiscuous genes are involved in T cell tolerance and autoimmunity in the thymus. Here, we identified Aire-expressing fibroblastic reticular cell (FRC), which was derived from mouse lymph node and also expressed in insulin promiscuous antigen. The expression of insulin was increased in cultured FRC over-expressed with Aire. These data suggest that Aire regulates promiscuous gene expression in FRC, and that this function might be under peripheral selection control.