• Parasites, Hosts and Diseases
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• v.50 no.4
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• pp.295-299
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• 2012

The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

• Parasites, Hosts and Diseases
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• v.52 no.4
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• pp.383-389
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• 2014

Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.542-544
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• 2002

This study was carried out to evaluate the ciliates Euplotes sp. as a live food for marine fish larvae. The ciliates and the rotifers Brachionus plicatilis, which were cultured with the baker's yeast Saccharomyces cerevisiae and the $\omega$-yeast emulsified with cuttle fish liver oil, were supplied to the larvae of flounder Paralichthys olivaceus and grouper Epinephelus akaara. Considering the size difference between the ciliates 68 $\cdot$ 7 $\mu$n and the rotifers 160 $\cdot$ 20 $\mu$n, the rotifers and ciliates were supplied to the larvae tank with the density of 2 inds./mL and 20 inds./mL, respectively. The survival rate and growth in length of the flounder larvae fed on rotifer were significantly higher than those on Euplotes sp.. In grouper larvae which have a small mouth diameter, even the survival rate of the larvae fed on the ciliates was better than that on the rotifers, it was very low less than $20\%$. Therefore, Euplotes sp. seem to be incongruent as a live food for marine fish larvae.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.95-98
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• 2013

The experiment was conducted to determine the value of black soldier fly (Hermetia illucens) larvae and pupae as feed supplements for fresh-water fish (Carassius auratus). Black soldier fly larvae grown on food waste were fed to fresh-water fish, live larvae alone, dried larvae, dried pupae and combination with commercial fish diet. A 16-week feed trial was conducted to determine whether black soldier fly larvae could be used as a feed supplement in fresh-water fish. The growth performance indicated that fish fed the live larvae ranked consistently higher, and in combination with commercial fish diet and dried larvae and pupae were next higher. The finding of this study suggest that black soldier fly larvae and pupae may be valuable feedstuff in commercial fish production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.1
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• pp.72-78
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• 2018

Fish eggs and larvae were collected in July and November of 2013 and January and May of 2014 to determine seasonal variation in the species composition of ichtyoplankton. Samples were classified based on morphological characteristics. Fish eggs were identified as belonging to five taxa; the most dominant species of fish eggs was Engraulis japonicus (62.05%) followed by Sardinops melanostictus (21.02%) and Leiognathus nuchalis (13.71%). These three species accounted for 96.8% of the total number of fish eggs collected. Larvae of 17 species in six families, and six orders were collected. The most dominant species of fish larvae was E. japonicus (51.79%), followed by L. nuchalis (12.59%) and Omobranchus elegans (12.08%). These three species accounted for 76.46% of the total number of larvae collected. Jinhae Bay was identified as a major spawning and nursery ground for fish species inhabiting in the south coast of Korea, such as E. japonicus, L. nuchalis, S. melanostictus, and Omobranchus elegans. Therefore, management of marine fish resources in the South Sea should necessarily include the ecological management of Jinhae Bay.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.1-7
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• 1968

Formerly T. Nishimura, T. Oshima and M. Otsuru studied on Anisakis-like larvae in the various marine fishes from the Japan Sea and the Pacific Ocean. In the present observation, many kinds of marine fishes from the Yellow Sea and the southern coast of Korea were. studied, with the samples from the islands: Eocheng-do, Sohuksan-do, Chuja-do and Keomun-do. The authors examined 313 samples from the adjacent waters of Korea and the distribution of the Anisakis-like larvae in the fish body was found to be 9,219 larvae of Anisakis-like larvae found in the mesentery and coelomic cavity of all samples. Of them, 8,112 Anisakis-like larvae were found from 187 samples from the southern coast of Korea, 1,107 Anisakis-like larvae were found in 126 samples from the Yellow sea (Tables 1 & 2). The fish from the southern coast of Korea are much more infected than the fish from. the Yellow Sea. The larger the fish length, the heavier infection of Anisakis-like larvae were observed.

• Parasites, Hosts and Diseases
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• v.49 no.1
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• pp.39-44
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• 2011

The infection status of marine fish and cephalopods with Anisakis simplex third stage larva (L3) was studied over a period of 1 year. A total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the Cooperative Fish Market in Busan, Korea, from August 2006 to July 2007. They were examined for A. simplex L3 from the whole body cavity, viscera, and muscles. A. simplex L3 were confirmed by light microscopy. The overall infection rate reached 34.3%, and average 17.1 larvae were parasitized per infected fish. Fish that recorded the highest infection rate was Lophiomus setigerus (100%), followed by Liparis tessellates (90%), Pleurogrammus azonus (90%), and Scomber japonicus (88.7%). The intensity of infection was the highest in Gadus macrocephalus (117.7 larvae per fish), followed by S. japonicus (103.9 larvae) and L. setigerus (54.2 larvae). Although abundance of A. simplex L3 was not seasonal in most of the fish species, 10 of the 16 selected species showed the highest abundance in February and April. A positive correlation between the intensity of L3 infection and the fish length was obvious in S. japonicus and G. macrocephalus. It was likely that A. simplex L3 are more frequently infected during the spring season in some species of fish. Our study revealed that eating raw or undercooked fish or cephalopods could still be a source of human infection with A. simplex L3 in Korea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.4
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• pp.461-477
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• 2022

We investigated the community structure and performed detailed characterization of fish larvae assemblages collected from the southern coast of Korea in the spring and summer of 2021. The total abundance of fish larvae varied from 193.6 to 1,915.6 ind. 10 m^-3. The species were distributed across 10 orders with 23 families, and 41 taxa. The dominant taxa were Gobiidae spp., Engraulis japonicus, Nibea albiflora, Sebastiscus spp., Callionymus valenciennei, Pennahia argentata, Sebastes thompsoni, Parablennius yatabei, and Platycephalus indicus. Engraulis japonicus individuals were collected from April to August and their presence contributed greatly to the total abundance of fish larvae. The total number and abundance of species peaked in early summer and the Shannon-Weaver index was in the range 0.11-1.49. Redundancy analyses revealed that the major environmental factors affecting the fish larvae assemblage differed according to the dominant taxa. Water temperature, zooplankton density, and Paracalanus parvus s. l. density were the key factors affecting the spatial and temporal distribution of fish larvae in the southern coast of Korea in spring and summer.

• Journal of Aquaculture
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• v.16 no.2
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• pp.84-87
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• 2003

Effect of artificial illumination on feeding by the rockfish Sebastes schlegeli receiving no synthetic feed in the cage of the Tongyong Marine Ranch was investigated by analysing the stomach contents of 20 individuals every month from October 1998 to July 1999. The fish was found to have fed on naturally available zooplanktons, i.e. Calanus sinicu, zoeae, amphipods and copepodites in October, fish larvae in November-December, nematodes in January, amphipods in February and again fish larvae from May to July. Despite increase in the weight of stomach contents from 260 mg/fish during the initial period of study to 2,173 mg/fish, as many as 70% of the fish were found to have empty stomach during March-April. Weight of stomach contents did not increase with increasing age of the fish. Occurrence frequency was 83-90% for C. sinicus during October-November and >50% for fish larvae during March-April and June-July.

• Parasites, Hosts and Diseases
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• v.35 no.1
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• pp.19-24
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• 1997

The infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in Bayuquan region, Yingko city nearby the coast of the Bohai Sea from may to August 1992. A total of 7,327 laivae were collected from 156 fish of 19 species and 8 squids of one species. The 3rd-stage larvae of Anisokis simplex were collected from 121 fish (63..4%) of 15 species (N : 191) and from 8 squids (14.8%) of one species (N : %4), and they were total, 5,992 (81.8%). Out of remaining 1,335 larvae, 154 (2.1%) were classified as Rvnnqscnris type B from 23 fish of 4 species, 1,013 (13.8%) as avnnosccris type C from 79 fish of 13 species, 164 (2.2%) as Hvsterothylacium China type V from 20 fish of 4 species, 3 (0.04%) as Raphinoscnris from 3 fish of 2 species and one was Pseunotewonovc necipiens larva. Key words: Bohai Sea, Anisokinae, larvae , Anisckis, HvsterothWLacium, Thwnnascaris, Raphinqscaris , Pseudoterranova.