• 제목, 요약, 키워드: Flammulina velutipes

검색결과 197건 처리시간 0.031초

팽이버섯 열수추출물이 마우스 비장세포와 대식세포의 증식 및 활성에 미치는 효과 (The Effects of Flammulina velutipes Water Extract on the Activation of Spleen Cell and Macrophage in Mice)

  • 김경옥;류혜숙
    • 한국식품영양학회지
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    • v.31 no.2
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    • pp.236-241
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    • 2018
  • Flammulina velutipes is an edible mushroom and contains a lot of fiber, vitamin $B_1$, $B_2$, niacin and folic acid. This study was conducted to explore the effects of the Flammulina velutipes mushroom on immune cells and immunity. Th1 cytokine productions as $IFN-{\gamma}$, $TNF-{\alpha}$, and IL-2 were measured in an activated macrophage by Flammulina velutipes water extract in seven concentrations (0, 5, 10, 50, 100, 250, 500, and $1,000{\mu}g/mL$). Also, the splenocyte proliferation index was measured at 48 hours after treatment of the Flammulina velutipes water extract in seven concentrations or mitogen, LPS and ConA. The $IFN-{\gamma}$ and $TNF-{\alpha}$ productions were increased by treatment of the Flammulina velutipes water extract. The $TNF-{\alpha}$ production was significantly higher in the $50{\sim}1,000{\mu}g/mL$ Flammulina velutipes water extract treated macrophages. The $IFN-{\gamma}$ production of macrophages treated with the Flammulina velutipes water extract increased significantly in all groups, and the highest $1000{\mu}g/mL$ concentration. The splenocyte proliferation index was enhanced when the $10{\sim}1,000{\mu}g/mL$ Flammulina velutipes water extracts were treated compared to the control. These primary results suggest that Flammulina velutipes may enhance the immune function by activation of the macrophage and spleen cell.

팽이버섯 에탄올 추출물의 기능적 특성 (Functional Activities of Ethanol Extracts from Flammulina velutipes)

  • 오세인;이미숙
    • 한국식품영양학회지
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    • v.23 no.1
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    • pp.15-22
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    • 2010
  • This study was conducted to evaluate the antioxidative effect and antimutagenic capacity of ethanol extracts of Flammulina velutipes by employing biological and biochemical assays. The $IC_{50}$ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Flammulina velutipes was found to be 28.39 mg/assay, 9.33 mg/assay and 144.61 mg/assay respectively. Therefore, the most effective antioxidative capacity of ethanol extracts of Flammulina velutipes was $Fe^+$-induced linoleate peroxidation, among the method used this study. The indirect and direct antimutagenic effects of the ethanol extracts of Flammulina velutipes were examined by the Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-anthramine and on direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 and mediated by 2-nitrofluorene in Salmonella typimurium TA98 were 0%, respectively. These findings indicate that ethanol extracts of Flammulina velutipes have no effects on indirect and direct mutagenicity. Based on these results, it believed that the ethanol extracts of Flammulina velutipes has antioxidative capacities, and is a the candidate for the prevention and dietetic treatment of chronic diseases and the development of antioxidative functional food.

Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

  • Kim, Jong-Kun;Park, Young-Jin;Kong, Won-Sik;Kang, Hee-Wan
    • Mycobiology
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    • v.38 no.4
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    • pp.331-335
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    • 2010
  • In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using the partial ftx gene (410 bp) along with the hygromycin B phosphotransferase gene (hygB) downstream of the glyceraldehydes-3-phosphate dehydrogenase (gpd) promoter. The plasmid was transformed into protoplasts of monokaryotic strain 4019-20 of F. velutipes by electroporation. High transformation efficiency was obtained with an electric-pulse of 1.25 kV/cm by using 177 transformants/${\mu}g$ of DNA in $1{\times}10^7$ protoplasts. PCR and Southern blot hybridization indicated that a single copy of the plasmid DNA was inserted at different locations in the F. velutipes genome by non-homologous recombination. Therefore, this transformation system could be used as a useful tool for gene function analysis of F. velutipes.

팽이버섯의 자실체형성 초기과정에서 특이적으로 발현하는 유전자의 클로닝 (Cloning of a Gene Specifically Expressed During Early Stage of Fruiting Body Formation in Flammulina velutipes)

  • 김둘이;동지칙
    • 한국균학회지
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    • v.27 no.3
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    • pp.187-190
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    • 1999
  • 팽이버섯의 자실체 분화 과정에서 특이적으로 발현하는 유전자 분리를 위한 cDNA library는 발이처리 후 7일째 배양한 균사체의 mRNA에 의해 만들어졌다. cDNA클론 FVFD16(Flammulina velutipes fruiting body differentiation)은 자실체 분화 과정에서 특이적으로 발현되는 클론으로 differential screening에 의해 선발되었다. Northern 분석에 의해 FVFD16의 발현 특성을 관찰한 결과, 1일과 4일째의 균사체에서 현저한 발현량을 나타내었다. FVFD16의 염기 서열을 검색한 결과, FVFD16의 mRNA는 open reading frame을 포함한 128의 아미노산 잔기(13.5kDa)를 가진 단백질로 추정되었다.

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Flammulina velutipes 항보체 활성다당의 생산에 미치는 영양원의 효과 (The Effect of Nutrients on the Production of Anti-Complementary Polysaccharide by Flammulina velutipes)

  • 이현경;신광순;송치현;성하진;양한철
    • 한국미생물·생명공학회지
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    • v.22 no.4
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    • pp.360-367
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    • 1994
  • The effect of various nutrients for the production of the anti-complementary polysaccha- ride by Flammulina velutipes, was examined and the partial purification of the polysaccharide was carried out. The culture conditions and medium compositions considerably influenced the anti-complementary activity of the polysaccharides. When a culture was carried out at 23$\circ$C and pH 5.5 for 6 days in the synthetic medium supplemented with galactose and NaNO$_{3}$, the production of the anti-complementary polysaccharide was maximized. The crude polysaccharide, FV-1 was isolated from the culture broth and partially purified into two fractions, FV-1-II and FV-1-III by gel filtration using Sephadex G-100. FV-1-II was mainly consisted of xylose, mannose, galactose and glucose, and rhamnose, mannose and galactose, for FV-1-III. Also, the anti-complementary activity of FV-1-III was reduced partially in the absence of the Ca ion. When crossed immunoelect- rophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the FV-1-III in the Ca ion free condition, a cleavage of C3 precipitin line was observed. These results indicate that the mode of complement activation by polysaccharide from Flammulina velutipes is via not only the classical pathway but also the alternative pathway.

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Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

  • Cho, Jung-Hee;Lee, Seung-Eun;Chang, Who-Bong;Cha, Jae-Soon
    • Mycobiology
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    • v.34 no.2
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    • pp.104-107
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    • 2006
  • Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.

팽나무버섯의 항암(抗癌) 성분(成分)에 관한 연구(硏究)(제(第)2보(報)) - 액내(液內) 배양(培養)에 의한 항암(抗癌) 성분(成分)의 생성(生成) - (Studies on Antitumor Components of Flammulina velutipes of Korea (II) - Production of Antitumor Component of Flammulina velutipes by Submerged Culture -)

  • 우명식
    • 한국균학회지
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    • v.11 no.4
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    • pp.147-150
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    • 1983
  • To investigate a possibility of producing the antitumor component by shake culture method, the mycelia of Flammulina velutipes were cultured in flasks on a shaker at $26{\sim}28^{\circ}C$ at 180 rpm for seven days. The extract of the mycelia was concentrated under vacuum. The precipitate obtained by adding a three-fold volume of ethanol was centrifugated and freeze-dried after dialysis. The fraction was tested against sarcoma 180 in the mice. The inhibition ratio of the fraction against the tumor was 68.0% at the dose of 20 mg/kg/day for the period of ten days and the tumors in three of the mice were completely regressed. The results showed, therefore, that the antitumor component was produced by the shake culture method.

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저장 조건을 달리한 팽이버섯의 영양학적 특성 평가 (Evaluation of the nutrition properties of Flammulina velutipes)

  • 김경제;진성우;최봉석;김진경;고영우;반승언;서경순
    • 한국버섯학회지
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    • v.14 no.2
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    • pp.44-50
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    • 2016
  • 팽이버섯 포장필름의 국산화 및 동남아시아 지역을 대상으로 한 수출용 필름을 개발하기 위하여, 개발된 포장필름을 현재 시중에 유통 중인 포장필름 및 수입산 포장필름과 함께 저장 중 팽이버섯의 저장 온도별 품질변화에 이어 유리당, 유리아미노산, 구성아미노산 등에 대한 영양학적 특성변화를 검토하였다. 유리당 함량을 분석한 결과, 저장 온도가 높아질수록 유리당 함량이 약간씩 증가하는 경향을 보였으나, 포장 필름의 종류에 따라서는 큰 차이를 나타내지 않았다. 유리아미노산의 경우 저장온도가 올라감에 따라 methionine은 증가하는 반면, tyrosine은 감소하였다. Polyphenol oxidase의 활성 확인결과 본 과제로 인해 개발된 포장지가 우수함을 확인하였다. 베타글루칸 함량은 건강 기능성 식품의 표준 지표로 사용되는 성분이나 버섯 저장성을 판단하는 지표로 사용하기에는 적당하지 않을 것으로 여겨진다.

팽이버섯 (Flammulina velutipes) 계통의 분류를 위한 SSR 마커개발 (Development of SSR markers for classification of Flammulina velutipes strains)

  • 우성이;서경인;장갑열;공원식
    • 한국버섯학회지
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    • v.15 no.2
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    • pp.78-83
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    • 2017
  • 버섯과에서 한국, 중국, 일본에서 재배 또는 수집하여 농촌진흥청에 보관 중인 32개의 팽이버섯 계통에 대하여 조사하였다. 팽이버섯의 미소반복서열(microsatellite)을 포함하고 있는 490개의 DNA 단편을 얻었다. 다양한 팽이버섯 균들의 PCR을 통한 DNA 프로파일링을 수행함으로써 다형성 변이가 많이 검출되었다. 총 34개의 대립 유전자가 12개의 다형성 SSR 마커 중에서 검출되었고, 평균 3.42개의 대립 유전자와 대립 유전자의 수는 유전자좌당 2개에서 7개까지 분포하였다. 대립 형질 빈도는 0.42(GB-FV-127)에서 0.98(GB-FV-166)이었으며 이형접합체 관측치($H_O$)와 기대치($H_E$)는 각각 0.00에서 0.94(평균 = 0.18)와 0.03에서 0.67(평균 = 0.32)이었다. 다형성 지수는 (PIC) GB-FV-127 마커에서 가장 높은 0.61, 평균대립 유전자 수는 5를 나타내었고, GB-FV-166마커에서 0.03과 2로 가장 낮았다. 본 연구에서 평균 PIC 값(0.29)은 대립 유전자의 평균 수(3.42)로 관찰되었다. 결론적으로 우리는 풍부한 SSR 라이브러리에서 12 개의 다형성 SSR 마커를 개발하는데 성공했다. 이러한 SSR은 계통 발생 분석, 유전적 변이 평가에 중요하게 사용될 것이다.

Flammulina velutipe의 국내 균주와 외래 균주 간의 ITS region을 이용한 계통학적 유연관계 분석 (A Phylogenetic Relationship between Foreign and Korean Strains of Flammulina velutipes Identified by rDNA-ITS Sequence Analysis)

  • 황광립;우주리;윤혁준;이창윤;이상한;공원식;김종국
    • 생명과학회지
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    • v.22 no.1
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    • pp.62-73
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    • 2012
  • 본 연구는 팽이버섯(Flammulina velutipes)의 국내 균주와 외래 균주간의 유전적 유연관계를 조사하기 위하여 수행되었으며, 계통수 측정 결과 3개 그룹으로 나눠짐을 확인하였다. 20개 F. velutipes 균주들의 ITS region 염기 서열을 확보하였으며, 이들 서열을 바탕으로 multiple alignment를 보았으며, Neighbor-joining method를 이용하여 계통수를 작성하였으며, 2개 그룹으로 나눠짐을 확인하였다. 또한 F. velutipes 4154 균주의 rDNA-cluster를 최초로 분석한 결과, 총 10,974 bp임을 밝혔다. SSU는 1,806 bp, ITS region은 553 bp의 염기배열이 결정되었다. ITS 1 부분은 245 bp이고 ITS 2는 308 bp였으며, LSU에 해당되는 염기서열은 3,402 bp, IGS 1은 1,400 bp, 5S는 83 bp, IGS 2는 3,571 bp임을 확인하였다.