• Title, Summary, Keyword: Flammulina velutipes

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Molecular Marker Related to Fruitbody Color of Flammulina velutipes

  • Kong, Won-Sik;You, Chang-Hyun;Yoo, Young-Bok;Kim, Gyu-Hyun;Kim, Kwang-Ho
    • Mycobiology
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    • v.32 no.1
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    • pp.6-10
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    • 2004
  • White and brown strains of Flammulina velutipes were inter-crossed. All $F_1$ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

Effects of Addition of Cathamus tinctorius, Coptis japonica and Asarum sieboldii to Sawdust substrate on the Growth and Development of Winter Mushroom, Flammulina velutipes (홍화, 황련, 세신 배지첨가제가 팽이버섯 생육에 미치는 영향)

  • 최인영
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.131-135
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    • 1998
  • Cathamus tinctorius, Coptis japonica and Asarum sieboldii were tested as substrate for the production of Flammulina velutipes. Among the C. tinctoris , C. japonica and A. sieboldii , C. tinctoris was the best substrate for the production of fruitbody. The effects of addition of C. tinctoris to sawdust substrate resulted in the increased mycelial growth on inoculum culture, 3.1% in ratio of fully culture and shorted one day in culture period. C. tinctoris was decreased 6.1% in ratio of fully culture, 11.0% in ratio of fruitbody productive culm. The addition of C.tinctoris, C.japonica to sawdust substrate increased 134.6%, 114.1% on the yield of the mushroom fruitbody respectively . But A. sieboldii decreased the mycelial growth and pineheading ratio delayed the production of fruitbody.

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Genome-wide Single Nucleotide Polymorphism-based Assay for Phylogenetic Relationship of the Flammulina velutipes (팽이버섯(Flammulina velutipes)의 Genome-wide SNP (Single Nucleotide Polymorphism)에 의한 계통 분석)

  • Woo, Sung-I;Kim, Eun-Seon;Han, Jae-Gu;Jang, Kab Yeul;Shin, Pyung-Gyun;Oh, Youn-Lee;Oh, Min Ji;Jo, Sung-Hwan;Lee, Jeong-Hee;Kim, Kyung-Soo;Kong, Won-Sik
    • The Korean Journal of Mycology
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    • v.43 no.4
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    • pp.231-238
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    • 2015
  • Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.

Applicability of Spent Mushroom Media as Horticultural Nursery Media (버섯재배 후 탈병배지의 원예용 상토재료 이용성 검토)

  • Lee, Chan-Jung;Cheong, Jong-Chun;Jhune, Chang-Sung;Kim, Seung-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.2
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    • pp.117-122
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    • 2009
  • This study was carried out to investigate applicability of Spent Mushroom Media(SMM) as horticultural nursery media. After the mushroom has been harvested, the SMM contains a lot of organic material, different microorganism and high density of mushroom hypha. The pH, phosphate and exchangeable cation concentrations of SMM of Flammulina velutipes were higher than those of any other treatment. The CEC and $NH_4-N$ were the highest in SMM of bottle-cultivated oyster mushroom (Pleurotus ostreatus). Bacteria and fungi showed the highest density in SMM of Flammulina velutipes. Most dominant bacteria were Microbacterium sp., Rhodococcus sp. and Agrobacterium sp. in SMM of Flammulina velutipes and Bacillus sp., Pseudomonas sp., Curtobacterium sp. and Microbacterium sp. in that of Pleurotus eryngii. The SMM contained high density of mushroom hypha that inhibited germination of seed and growth of young seedlings. Therefore, composting process of the SMM is indispensible to decline of vitality of mushroom hypha. The SMM of Flammulina velutipes with 0~30% vermiculite showed high germination rate in red pepper and chinese cabbage seeds. SMM of Pleurotus eryngii with 20% vermiculite showed 100% germination rate in red pepper seeds, but chinese cabbage seeds nearly failed to germinate with 30% vermiculite. The growth of red pepper was increased according to increasing mixture ratio of vermiculite. Accordingly, we concluded that SMM of Flammulina velutipes contained 0~30% of vermiculite can be used to horticultural growth bed for red pepper.

Cloning and Expression of Leu 2 Gene (${\beta}-isopropylmalate$ dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli (팽나무버섯 균사체에서 ${\beta}-isopropylmalate$ dehydrogenase(Leu 2) gene 의 cloning 및 E. coli에서 발현)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.35-38
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    • 1989
  • Gene libraries of DNA from Flammulina velutipes were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding ${\beta}-isopropylmalate$ dehydrogenase from F. velutipes was cloned by complementation of leucine requiring mutant of E. coli. The size of inserted DNA fragment of this clone is about 1 Kbp. The fragment has Bam H1 and Ava 1 restriction sites.

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Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes

  • Yun, Yeo Hong;Koo, Ja Sun;Kim, Seong Hwan;Kong, Won Sik
    • Mycobiology
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    • v.43 no.3
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    • pp.327-332
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    • 2015
  • Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.

Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes

  • Kim, Hong-Il;Kwon, O-Chul;Kong, Won-Sik;Lee, Chang-Soo;Park, Young-Jin
    • Mycobiology
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    • v.42 no.4
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    • pp.322-330
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    • 2014
  • The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome, four new laccase genes (fvLac-1, fvLac-2, fvLac3, and fvLac-4) were found to contain four complete copper-binding regions (ten histidine residues and one cysteine residue) and four cysteine residues involved in forming disulfide bridges, fvLac-1, fvLac-2, fvLac3, and fvLac-4, encoding proteins consisting of 516, 518, 515, and 533 amino acid residues, respectively. Potential N-glycosylation sites (Asn-Xaa-Ser/Thr) were identified in the cDNA sequence of fvLac-1 (Asn-454), fvLac-2 (Asn-437 and Asn-455), fvLac-3 (Asn-111 and Asn-237), and fvLac4 (Asn-402 and Asn-457). In addition, the first 19~20 amino acid residues of these proteins were predicted to comprise signal peptides. Laccase activity assays and reverse transcription polymerase chain reaction analyses clearly reveal that $CuSO_4$ affects the induction and the transcription level of these laccase genes.

Isolation of 1',3'-Dilinolenoyl-2'-Linoleoylglycerol with Tyrosinase Inhibitory Activity from Flammulina velutipes

  • Jang, Se-Gul;Jeon, Kyung-Su;Lee, Eun-Hee;Kong, Won-Sik;Cho, Jae-Yong
    • Journal of Microbiology and Biotechnology
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    • v.19 no.7
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    • pp.681-684
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    • 2009
  • This study was carried out to evaluate the inhibitory effect of Flammulina velutipes extracts on tyrosinase activity and to identify its biologically active component. The ethyl acetate and n-butanol extracts showed potent tyrosinase inhibitory activities. Subsequently, fractions of the n-butanol extract showed only a partial tyrosinase inhibitory activity. The most active compound of tyrosinase inhibitory activity was identified from the ethyl acetate extract as 1',3'-dilinolenoyl-2'-linoleoylglycerol (LnLLn) by comparing its mass, $^1H-$, and $^{13}C-NMR$spectral data with those previously reported in the literature. LnLLn showed tyrosinase inhibitory activity with an $IC_{50}$value of 16.1 ${\mu}g/ml$. These results suggest that the ethyl acetate extract of F. velutipes could be applicable for the development of a new whitening agent.

Comparative Analysis of Expressed Sequence Tags from Flammulina velutipes at Different Developmental Stages

  • Joh, Joong-Ho;Kim, Kyung-Yun;Lim, Jong-Hyun;Son, Eun-Suk;Park, Hye-Ran;Park, Young-Jin;Kong, Won-Sik;Yoo, Young-Bok;Lee, Chang-Soo
    • Journal of Microbiology and Biotechnology
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    • v.19 no.8
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    • pp.774-780
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    • 2009
  • Flammulina velutipes is a popular edible basidiomycete mushroom found in East Asia and is commonly known as winter mushroom. Mushroom development showing dramatic morphological changes by different environmental factors is scientifically and commercially interesting. To create a genetic database and isolate genes regulated during mushroom development, cDNA libraries were constructed from three developmental stages of mycelium, primordium, and fruit body in F. velutipes. We generated a total of 5,431 expressed sequence tags (ESTs) from randomly selected clones from the three cDNA libraries. Of these, 3,332 different unique genes (unigenes) were consistent with 2,442 (73%) singlets and 890 (27%) contigs. This corresponds to a redundancy of 39%. Using a homology search in the gene ontology database, the EST unigenes were classified into the three categories of molecular function (28%), biological process (29%), and cellular component (6%). Comparative analysis found great variations in the unigene expression pattern among the three different unigene sets generated from the cDNA libraries of mycelium, primordium, and fruit body. The 19-34% of total unigenes were unique to each unigene set and only 3% were shared among all three unigene sets. The unique and common representation in F. velutipes unigenes from the three different cDNA libraries suggests great differential gene expression profiles during the different developmental stages of F. velutipes mushroom.

Comparison of Physico-chemical and Nutritional Characteristics of Pre-inoculation and Post-harvest Flammulina velutipes Media (팽이버섯 접종전과 수확후 배지의 이화학성 및 영양성분 비교)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Suh, Jang-Sun;Moon, Yea-Hwang
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.174-178
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    • 2012
  • This study were carried out to analyze nutritional ingredients of pre-inoculation and post-harvest Flammulina velutipes media. pH, moisture content, total carbon(T-C), total nitrogen(T-N), crude protein, crude fiber, and crude fat content on the 8 raw materials using Flammulina velutipes bottle cultivation medium, and after sterilization(pre-inoculation) and post-harvest medium was analyzed. This result is expected to be utilized as the basis for recycling post-harvest media.