• Title, Summary, Keyword: Flammulina velutipes

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The contamination check before inoculation at the liquid Spawn on Flammulina velutipes (팽나무버섯 액체 종균의 접종 전 오염 검사)

  • Shim, Kyu-Kwang;Yoo, Young-Jin;Koo, Chang-Duck;Kim, Myung-Koon
    • Journal of Mushroom
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    • v.10 no.1
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    • pp.44-48
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    • 2012
  • In this study, whether Giemsa staining solution can accurately determine bacterial contamination of liquid spawn for Flammulina velutipes in a short period of time was investigated. Giemsa solution staining cells of blood, bone marrow, lymph node, malaria parasites, rickettsia et al. was prepared by dissolving basic methylene azul and methylene blue, and acidic eosine in methyl alcohol-glycerine. Supernatant samples of Flammulina velutipes liquid spawn cultured under explosive aeration were placed on a slide, mixed with Gimesa solution and examined with optical microscope after staining. In 40 to 60 seconds bacterial cells were distinguishable from soybean meal residual and hyphal cell fragments. Thus we conclude that microscopy using Gimesa staining solution is a quick, simple and accurate method for the mushroom growers to effectively use to detect bacterial contamination of the liquid spawn.

Comparison of mycelial growth and fruiting bodies yield according to substrate in Flammulina velutipes (팽이버섯의 배지조성에 따른 균사 배양 및 자실체 수량 비교)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Moon, Ji-Won
    • Journal of Mushroom
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    • v.12 no.2
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    • pp.117-121
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    • 2014
  • The experiments were conducted to provide information on the chemical concentrations and cultural characteristics in the periods of hyphal incubation, primordial formation, and fruiting bodies yield of winter mushroom, Flammulina velutipes at the mixture ratio of the raw materials. Substrates were analyzed for pH, total carbon (T-C), total nitrogen (T-N), and C/N ratio. In case of Flammulina velutipes, yield of fruiting bodies were 190.5 g/850 ml in the substrates, [Corn-cob + Rice bran + Soybean curd residue (75:20:5)], which was increased 20.4% more than the control, [Douglas fir sawdust + Rice bran (75:25)]. But the periods of hyphal incubation took 28 days, which was 7 days longer than the control. Also, in the substrates [Douglas fir sawdust + Rice bran + Soybean curd residue (75:20:5)], the yields was 172.7 g/850 ml and the periods of hyphal incubation was 21 days.

Effects of Addition of Apple pomace to sawdust substrate on the Growth and Development of Flammulina velutipes (사과가공부산물 첨가배지가 팽이버섯(Flammulina velutipes)의 균사생장과 자실체에 미치는 영향)

  • Jo, Woo-Sik;Yun, Yeong-Seok;Rew, Young-Hyun;Park, Sun-Do;Choi, Boo-Sull
    • The Korean Journal of Mycology
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    • v.24 no.3
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    • pp.223-227
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    • 1996
  • The effects of addition of dried apple pomace to sawdust substrate on the growth of Flammulina velutipes were investigated during the period of 2 years $(1994{\sim}1995)$. Dried apple pomace used in this study consisted of 44.6% C, 0.54% N, 0.16% CaO, 0.16% $P_2O_5$, 0.11% MgO and 1.29% $K_2O$ (pH 4.4). The addition of 15% apple pomace (v/v) increased the yield of the mushroom fruitbody by 9%, and period of primordia formation was similar regardless of the treatments. In economical analysis, the addition of 15% apple pomace (v/v) increased 29% compared to the control treatment in relative income.

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Standardization of Chemically Defined Medium for the Production of Mycelium and Basidiocarps in Flammulina velutipes (팽나무버섯 균사체 및 자실체 생산을 위한 화학합성배지의 최적화)

  • Song, Chi-Hyeun;Lee, Chang-Ho;Ahn, Jang-Hyuk;Hong, Bum-Shik;Yang, Han-Chul
    • The Korean Journal of Mycology
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    • v.23 no.1
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    • pp.53-60
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    • 1995
  • Nutritional requirements for the growth of Flammulina velutipes were studied. Mannitol, glutamic acid and ammonium nitrate were chosen for the maximum mycelial growth when various carbon and nitrogen sources tested. Optimum C : N ratio for the mycelial growth was 20 : 1. Potassium dihydrogen phosphate was selected among the phosphate sources. Magnesium sulphate and thiamine HCl stimulate mycelial growth. Final compositions of optimized chemically defined medium were 1.5% mannitol, 0.082% $NH_4NO_3$, 0.312% glutamic acid, 0.25% $KH_2PO_4$, 0.06% $MgSO_4{\cdot}7H_2O\;and\;0.3\;{\mu}g/l$ thiamin HCl. This medium not only support mycelial growth but also induce fruit body formation.

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Quality Properties of Fish Meat Paste Containing Enoki Mushroom (Flammulina velutipes) (팽이버섯 함유 어묵의 물성 특성)

  • 이승철;구성근;류연경;황영만;하정욱
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.2
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    • pp.288-291
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    • 2001
  • To provide functional properties in fish meat paste, enoki mushroom (Flammulina velutipes) having anticarcinogenic effect and reduction of blood pressure was added at 1, 5, 10, 15, and 20%. The fried fish meat pastes were investigated for color, rheological properties, and sensory evaluation. Increasing the amount of mushroom in the fish meat paste tended to decrease the lightness (L) in Hunter color value, but to increase the redness (a) and yellowness (b). All test samples with 3mm thickness had good flexibility and did not break even after 4 times folds. The sample containing 15% mushroom showed the highest values in strength, gumminess and brittleness. In sensory evaluation, the higher amounts of mushroom obtained favorite score in color and flavour and 5% mushroom sample had the best score in overall acceptance. Therefore, this results suggest that enoki mushroom can be applied to fish meat paste for the purpose of high quality and functionality.

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Antioxidant and Anticancer Effects of Water Extract from Pleurotus eryngii, Flammulina velutipes (새송이버섯, 팽이버섯 열수추출물의 항산화 및 항암 활성)

  • Ryu, Hye Sook;Kim, Soo Hyun;Jeon, Moon Hee;Choi, Hae Yeon
    • The Korean Journal of Food And Nutrition
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    • v.31 no.6
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    • pp.911-918
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    • 2018
  • Various studies on the effect of mushroom, a member of phytonutrients, on inflammatory diseases have been reporter over the years, Among various species mushrooms, King oyster mushroom (Pleurotus eryngii) and winter mushroom (Flammulina velutipes) are popular dietary ingredients for Asian cuisine. The extracts from all the three mushrooms were used to determine the antioxidative effect and the cytotoxicity. Result analysis were repeated more than three times to get an average${\pm}$standard deviation, and statistical significance were confirmed by SPSS. As a result, total phenol content of the king oyster mushroom and the winter mushroom were $19.66{\pm}0.10mg/g$ and $22.08{\pm}1.10mg/g$, respectively. Also, the total flavonoid content was $15.21{\pm}1.31mg/g$ for the king oyster mushroom and $20.50{\pm}4.52mg/g$ for winter mushroom. The results in winter mushroom showed higher values in total phenol and flavonoid content than in the king oyster mushroom. All samples of extracts showed free radical DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity with their 10 mg/mL concentration. These results indicate that the extract of the king oyster mushroom (Pleurotus eryngii) and the winter mushroom (Flammulina velutipes) may be possible phytonutrients with the anti-inflammatory and the anti-cancer effect.

Development of Substrates for the Production of Basidiocarps of Flammulina velutipes (팽나무버섯 자실체 생산을 위한 기질개발)

  • Song, Chi-Hyeun;Lee, Chang-Ho;Huh, Tae-Lin;Ahn, Jang-Hyuk;Yang, Han-Chul
    • The Korean Journal of Mycology
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    • v.21 no.3
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    • pp.212-216
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    • 1993
  • Agricultural and forest wastes were tested as substrate for the production of Flammulina velutipes. Among the meranti, pine tree, mixed sawdust, coffee waste and peanut hull tested, coffee waste was the best basal substrate for the production of basidiocarps. When various supplements, such as ricebran, brewer's grain, defatted corn flour, defatted soybean flour and defatted rapeseed flour were tested, defatted corn flour was chosen for the production of basidiocarps. Maximum yield of basidiocarps(129.38 g/bottle) was obtained from the mixed medium at 4 : 1 ratio of coffee waste and defatted corn flour.

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Breeding of Flammulina velutipes Strains Adaptable to Elevated-temperature

  • Kong, Won-Sik;Cho, Yong-Hyun;Jhune, Chang-Sung;Yoo, Young-Bok;Kim, Kwang-Ho
    • Mycobiology
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    • v.32 no.1
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    • pp.11-16
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    • 2004
  • Winter mushroom, Flammulina velutipes, needs low temperature during its cultivation. To save on farm costs, especially during summer, a strain adaptable to a higher or elevated-temperature must be developed. At the start of breeding program, parental strains which could endure high temperature were obtained. Seuenty four dikaryotic strains were collected and divided into four groups according to the nature of temperature. They also had different fruiting temperature. Finally we selected three brown strains ASI 4048, 4057 and 4072, and collected their spores. These selected strains can germinate even at a high temperature of $32^{\circ}C$, which were dramatically higher than the other strains. Based on these results, the new white strain adapted to mid-temperature by backcross mating was developed. Molecular markers were applied to select white fruitbody producing strains without cultivation. They showed a specific band which co-segregated with brown fruitbody forming strains in $BC_1F_1$ progenies. Selected white strains were tested under several elevated temperature conditions.

Antioxidative Effects of Mushroom Flammulina velutipes Extract on Polyunsaturated Oils in Oil-in-water Emulsion

  • Jang, Mi-Soon;Park, Hee-Yeon;Ushio, Hideki;Ohshima, Toshiaki
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.604-609
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    • 2009
  • The antioxidative activities of the water solution of crude extract from edible mushroom enokitake Flammulina velutipes were compared with those of ascorbic acid and ascorbic acid 6-palmitate in oil-in-water (O/W) emulsions of cod liver oil. Oxidation of the emulsions was carried out at 40 and $50^{\circ}C$ in the dark. The antioxidant activities were measured by in vitro assay against oxygen uptake, 2-thiobarbituric acid value, hydroperoxide formation of the oils. Also, residual docosahexaenoic acid content was measured as indices of lipid oxidation. The cod liver oil in O/W emulsions with added enokitake crude extract (ECE) was significantly more stable against lipid oxidation than the control emulsions without the extract in terms of any oxidation indices used. Moreover, ECE provided remarkable antioxidative properties to eicosapentaenoic acid ethyl ester in emulsion system. These observations demonstrate that F. velutipes can be used as a natural antioxidant, which effectively prevents oxidation of polyunsaturated oils in emulsion system.

Optimization of Microwave Extraction Conditions for Flammulina velutipes by Response Surface Methodology (반응표면분석에 의한 팽이버섯의 microwave 추출조건 최적화)

  • Kim, Hyun-Ku;Kim, Mi-Ok;Choi, Mal-Gum;Kim, Kong-Hwan
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.222-228
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    • 2003
  • Optimum extraction conditions for yield, browning color, electron-donating ability, nitrite-scavenging effect, total polyphenol content, and tyrosinase-inhibitory activity of Flammulina velutipes were determined using response surface methodology through central composite design. Yield of F. velutipes was affected by ethanol concentration, and browning color improved more with the increase of ethanol concentration than microwave power. Nitrite-scavenging effect was improved with increasing ethanol concentration and decreasing microwave power. Electron-donating ability and total polyphenol content were improved with increasing ethanol concentration and microwave power. Tyrosinase-inhibitory effect increased more with decrease in microwave power than with ethanol concentration. The optimal range of extraction conditions for effective component of F. velutipes were $47.21{\sim}76.05$ watt of microwave power, $10.25{\sim}43.56%$ of ethanol concentration, and 5.72 min of extraction time.