• Title, Summary, Keyword: Flammulina velutipes

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Transformation of the ${\beta}-Isopropylmalate$ Dehydrogenase Gene of Flammulina velutipes into Pleurotus florida (팽나무버섯 Leu 2 gene(${\beta}-isopropylmalate$ dehydrogenase)의 사철 느타리버섯 형질전환)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.27-30
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    • 1989
  • F. velutipes Leu 2 gene (${\beta}-isopropylmalate$ dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.

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Effect of Inoculation Time and Population Density of Pseudomonas agarici and P. tolaasii on the Mycelial Growth and the Fruit body Formation in Flammulina velutipes (Pseudomonas agarici와 P. toluaasii의 접종시기 및 접종농도가 팽이버섯의 균사생장과 자실체형성에 미치는 영향)

  • Lee, Hyun-Uk;Moon, Byung-Ju;Lee, Heung-Su;Cha, Heung-Oh;Cho, Dong-Jin;Shin, Won-Kyo
    • The Korean Journal of Mycology
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    • v.26 no.1
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    • pp.60-68
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    • 1998
  • The effect of P. agarici and P. tolaasii causing the bacterial disease of mushrooms on the mycelial growth and fruitbody formation of F. velutipes was evaluated in laboratory. When the pathogenic bacteria was inoculated simultaneously with F. velutipes or 5 days after inoculation of F. velutipes, they significantly deterred both mycelial growth and fruitbody formation of F. velutipes in sawdust culture and showed strong inhibition under high population density. They appeared to be tender or milky in exhibiting symptom on F. velutipes by inoculating the concentration of $10^2{\sim}10^6$ of unit/g media, and their growth seemed to be stopped under $10^8\;cfu/g$ media. On $10^2\;cfu/g$ media of P. agarici and $10^4\;cfu/g$ media of P. tolaasii, there was no effect on the fruitbody yield of F. velutipes. P. tolaasii was more suppressive in the mycelial growth of F. velutipes than P. agarici, while on fruitbodies formation of F. velutipes, P. agarici showed slightly higher inhibition than that of P. tolaasii. When the bacteria was inoculated 10 days after inoculation of F. velutipes, both mycelial growth and fruit body formation were not affected nearly.

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Forest Green Mold Disease Caused by Trichoderma pseudokoningii in Winter Mushroom, Flammulina velutipes (Trichoderma pseudokoningii에 의한 팽이버섯 푸른곰팡이병)

  • Choi, In-Young;Lee, Wang-Hyu;Choi, Joung-Sik
    • The Korean Journal of Mycology
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    • v.26 no.4
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    • pp.531-537
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    • 1998
  • Forest green mold incidence rate, extent of damage according to the inoculation periods, and its cultural characteristics were observed in the automatic cultural system of the winter mushroom, Flammulina velutipes. The incidence rate of the forest green mold was 7.7% in early cultivation stage and slowly increased to 14.9% in harvest stage. When the forest green mold was inoculated at cultural period, the rate was recorded at 100%, but the extent of the damage increased up to 40% (+++). There was also 100% incidence rate at early pinheading time, whereas the yield of mushroom decreased to ++ $(10{\sim}39%)$. The rate of forest green mold was greatly decreased to 34.4% at 10 days after pinheading, and its damage extent was also below 10%. A pathogen to infect the winter mushroom was identified as Trichoderma pseudokoningii. It's optimum temperature for mycelial growth is $25^{\circ}C$, and it grew 2.6 times faster than that of F. velutipes. The mycelial color of T. pseudokoningii was pale yellow or olivaceous in shades on PDA medium. Phialospore was one celled, and ellipsodal or obovoid, smooth walled, and measured $1.3{\sim}3.0{\times}1.0{\sim}2.5\;{\mu}m$. It aggregated in small heads at the tips of the phialides. The phialides were $3.2{\sim}9.2{\times}2.0{\sim}5.5\;{\mu}m$ and were of bowling pin type, solitary and alternate or more irregularly disposed at the conidiophore apex, T. pseudokoningii depressed the F. velutipes growth at the crossing cultivation when they were simultaneously. FV 4-1 (F. velutipes) cultivar was less depressed by T. pseudokoningii, but had a lower cross growth rate than the other four cultivars.

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Thermal Inactivation Parameters of Peroxidase in Flammulina velutipes and Lyophyllum ulmarium (팽이 및 만가닥버섯에서 추출한 peroxidase의 열 불활성화 특성)

  • Lee, Kyun;Kim, Kong-Hwan;Kim, Hyun-Ku
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1067-1072
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    • 2002
  • Peroxidase was used as a standard enzyme to determine optimum blanching conditions of Flammulina velutipes and Lyophyllum ulmarium. Crude peroxidase extracted from raw mushrooms had maximum activity at $10{\sim}15^{\circ}C$ and pH 5.5 (50 mM, potassium phosphate buffer) using substrates of $H_2O_2$ and p-Phenylendiamine. Thermal inactivation of the crude peroxidase followed the first-order kinetics. The activation energy and z value of the crude peroxidase for F. velutipes were 59.58 kcal/mol and $9.0^{\circ}C$, whereas were 43.05 kcal/mol and $12.4^{\circ}C$ for L. ulmarium, respectively. On the basis of thermal kinetics parameters obtained, the optimum blanching conditions for F. velutipes and L. ulmarium were 1 min at $70^{\circ}C$ and 5 min at $80^{\circ}C$, respectively. Activation energies and z values of peroxidases extracted from heat-treated mushrooms were 7.97 and 6.55 kcal/mol, and $59.8^{\circ}C\;and\;74.1^{\circ}C$ for F. velutipes and L. ulmarium, respectively.

Studies on Antitumor Components of Flammulina velutipes of Korea (I) (팽나무 버섯의 항암(抗癌) 성분(成分)에 관한 연구(硏究)(제 1보)(第 1報))

  • Woo, Myoung-Sik
    • The Korean Journal of Mycology
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    • v.11 no.2
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    • pp.69-77
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    • 1983
  • To find antitumor components with low toxicity in the Basidiomycetes of Korea, the carpophores of Flammulina velutipes (Fr.) Singer were extracted with hot water for eight hours. The extract was purified by dialyzing through Visking tube and a protein-bound polysaccharide fraction was obtained as pale brownish amorphous powder after freeze-drying. The fraction was examined for antitumor activity against sarcoma 180 implanted subcutaneously in the left groin in ICR mice. The inhibition ratio of this fraction against the tumor was 62.3% at the dose of 10mg/kg/day for the period of ten days. The tumors in three of the ten treated mice were completely regressed. The chemical analysis of the antitumor component by anthrone and Lowry-Folin methods showed that it consisted of a polysaccharide (42.4%) and a protein (24.5%). The hydrolysis of the polysaccharide moiety with 3% HCl-MeOH and trimethysilylation of the hydrolyzate yielded five monosaccharides which were identified by G.L.C. Several amino acids were identified by an amino acid autoanalyzer in the acid hydrolyzate of the protein moiety.

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Influences of Antibiotic Components Produced by Trichoderma spp. to Oyster Mushroom (Trichoderma속(屬)이 생산(生産)하는 항생물질(抗生物質)이 느타리버섯균(菌)에 미치는 영향(影響))

  • Kim, Myoung-Kon
    • The Korean Journal of Mycology
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    • v.13 no.2
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    • pp.105-109
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    • 1985
  • Isolates of the different species groups of Trichoderma from the mushroom culture beds were identified according to Rifai's classification and influence of antibiotics produced by them against the oyster mushroom was examined. Trichoderma islolates were identified as Trichoderma hamatum, Trichoderma viride and Trichoderma koningii. Among the Trichoderma isolates, fungistatic action of Trichoderma viride was found to be most remarkable. Pleurotus ostreatus and Pleurotus sajor-caju were the most susceptable of the edible mushrooms tested, followed by Lentinus edodes, Flammulina velutipes and Auricularia auricula. A needle-shaped crystal gained from the chloroform extract of the culture filtrate of Trichoderma viride repressed distinctively the mycelial growth of the oyster mushroom. The grade of repression of the crystal at 500ppm and 1/10 aequ­ous solution of the chloroform extract against the oyster mushroom, seemed equal to that of cycloheximide at $100{\sim}200ppm$.

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Characteristics and breeding of a new brown variety 'Geumhyang' with short cultivation period in Flammulina velutipes (재배기간이 짧은 갈색 팽이버섯 신품종 '금향'의 육성 및 특성)

  • Kim, Min-Ja;Chang, Who-Bong;Choi, Jae-Sun;Lee, Kwan-Woo;Joo, Gyeong-Nam;Kim, Yee-Gi
    • Journal of Mushroom
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    • v.13 no.2
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    • pp.92-96
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    • 2015
  • 'Geumhyang', a new variety with short cultivation period and yellowish ivory color in Flammulina velutipes, was bred by crossing between monokaryons isolated from 'Garlmoe' and wild strain 'CBMFV-33'. In bottle cultivation, the period of mycelial growth, primordia1 formation, and growth of fruit body was required 23 days, 7 days, and 17 days, respectively. Total culvivation period was 47 days, showing 7 days shorter than that of 'Garlmoe'. Pileus diameter was greater than that of 'Garlmoe', and pileus color showed yellowish ivory. Fruit body production per 850 ml bottle was 160 g, which was 8% higher than that of 'Garlmoe'. The taste, derived from 'Garlmoe' and wild strain, was excellent because of better chewing feeling than white ones. 'Geumhyang' with differentiated color and taste, has an advantage to target a niche market of white ones.

Characteristics and breeding of a new brown variety 'Heukhyang' with good taste in Flammulina velutipes (고식미 갈색 팽이버섯 신품종 '흑향'의 육성 및 특성)

  • Kim, Min-Ja;Chang, Who-Bong;Choi, Jae-Sun;Lee, Kwan-Woo;Joo, Gyeong-Nam;Kim, Yee-Gi
    • Journal of Mushroom
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    • v.13 no.2
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    • pp.103-107
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    • 2015
  • 'Heukhyang', a new variety with dark brown color, short cultivaltion period, and good taste in Flammulina velutipes, was bred by crossing between monokaryons isolated from 'Garlmoe' and wild strain 'CBMFV-10'. In bottle cultivation, the period of mycelial growth, primordia1 formation, and growth of fruit body was required 23 days, 6 days, and 17 days, respectively. Total culvivation period was 46 days, showing 8 days shorter than that of 'Garlmoe'. Pileus diameter and thickness were greater than those of 'Garlmoe', and pileus color showed dark brown. Fruit body production per 850 ml bottle was 135 g, which was 10% lower than that of 'Garlmoe'. However, the overall acceptance was most excellent in sensory evaluation, and 'Heukhyang' has an advantage to target a niche market of white ones due to differentiated color and taste.

Effect of Liquid Spawn on Flammulina velutipes Cultivation (팽이버섯 재배시 액체종균 사용 효과)

  • Ryu, Young-Hyun;Yoon, Young-Seok;Jo, Woo-Sik;Park, Sun-Do;Choi, Boo-Sull;Kim, Jong-Kuk
    • The Korean Journal of Mycology
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    • v.26 no.1
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    • pp.20-24
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    • 1998
  • The effects of liquid spawn to sawdust substrate on the growth of Flammulina velutipes were conducted for two years. The duration of optimal incubation for preculture and main culture of liquid spawn was 4 days and 6 days, respectively. When using liquid spawn, the application time for the first pinhead formation was similar with sawdust spawn, and incubation time of sawdust substrate was variable with liquid spawn and inoculum quantity. But, the overall yield of mushroom fruitbody was increased by using liquid spawn, excepting sesame hull extract medium.

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Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes (팽이버섯에서 분리된 FVFD16과 FVFD30 유전자의 게놈클론의 염기서열 및 특성)

  • Kim, Dool-Yi;Azuma, Tomo-Nori
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.26-31
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    • 2000
  • We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.

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