• Title/Summary/Keyword: Fragmentation Test

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Change of Interfacial properties by the Fiber Degradation in the Fiber Reinforced Composites (섬유강화 복합재료에서 섬유열화에 따른 계면특성의 변화)

  • Moon, Chang-Kwon;Kim, Young-Dae;Roh, Tae-Young
    • Journal of Ocean Engineering and Technology
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    • v.12 no.3 s.29
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    • pp.31-41
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    • 1998
  • Single fiber fragmentation technique was used to evaluate the change of interfacial properties by degradation of fiber tensile strength in the fiber reinforced composites. The influences of fiber tensile strength on the interfacial properties have been evaluated by the fragmentation specimens(weak fiber samples) of glass fiber/epoxy resin that was made using the pre-degraded glass fiber in distilled water at $80^{circ}C$ for specified periods. The effects of the immersion time on the interfacial properties in the distilled water at $80^{circ}C$ also have been evaluated by the fragmentation specimens(original fiber samples) of glass fiber/epoxy resin that was made using the received glass fiber. As the result, the tensile strength of glass fiber was decreased with the increasing of the treatment time in the distilled water at $80^{circ}C$ and the interfacial shear strength was independent of the change of the glass fiber strength in the single fiber fragmentation test. But in the durability test using the single fiber fragmentation specimen, interfacial shear strength decreased with the increasing of the immersion time in distilled water ar $80^{circ}C$. And it turned out that the evaluating of interfacial shear strength using original fiber tensile strength was valuable in the durability test for the water environment by the single fiber fragmentation technique.

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Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility

  • Javed, Aamir;Talkad, Muralidhar Srinivasaih;Ramaiah, Manjula Kannasandra
    • Clinical and Experimental Reproductive Medicine
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    • v.46 no.1
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    • pp.14-21
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    • 2019
  • Objective: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the participants were divided into fertile and infertile groups (50 patients in each group). Results: The alkaline comet test showed the best ability to predict male infertility, followed by the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, the sperm chromatin dispersion (SCD) test, and the sperm chromatin structure assay (SCSA), while the neutral comet test had no predictive power. For our patient population, the projected cut-off point for the DNA fragmentation index was 22.08% using the TUNEL assay, 19.90% using SCSA, 24.74% using the SCD test, 48.47% using the alkaline comet test, and 36.37% using the neutral comet test. Significant correlations were found between the results of the SCD test and those obtained using SCSA and TUNEL (r = 0.70 and r = 0.68, respectively; p< 0.001), and a statistically significant correlation was also found between the results of SCSA and the TUNEL assay (r = 0.77, p< 0.001). Likewise, the results of the alkaline comet test showed significant correlations with those of the SCD, SCSA, and TUNEL tests (r = 0.59, r = 0.57, and r = 0.72, respectively; p< 0.001). Conclusion: The TUNEL assay, SCSA, SCD, and the alkaline comet test were effective for distinguishing between fertile and infertile patients, and the alkaline comet test was the best predictor of male infertility.

The Effect of Joint Condition on Rock Fragmentation in Bench Blasting (절리간격과 방향이 벤치발파시 암석파쇄도에 미치는 영향에 대한 실험 연구)

  • Choi Yong-Kun;Lee Chung-In
    • Explosives and Blasting
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    • v.23 no.2
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    • pp.37-44
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    • 2005
  • Recent studies reported that natural block size of rock and joint orientation highly affect on rock fragmentation. In this study, blasting test using high strength cement mortar was carried out to verify this fact. The result of this test indicated that fragmentation is influenced by the joint interval, and at same joint interval condition, fragmentation depends on joint orientation. These results are significantly coincident with field investigations.

Microfailure Mechanisms of Single-Fiber Composites Using Tensile/Compressive Fragmentation Techniques and Acoustic Emission (인장/압축 Fragmentation시험법과 음향방출을 이용한 단 섬유 복합재료의 미세파괴 메커니즘)

  • 김진원;박종만;윤동진
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2000.04a
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    • pp.159-162
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    • 2000
  • Interfacial and microfailure properties of carbon fiber/epoxy matrix composites were evaluated using both tensile fragmentation and compressive Broutman tests with acoustic emission (AE). Amino-silane and maleic anhydride polymeric coupling agents were used via the dipping and electrodeposition (ED), respectively. Both coupling agents exhibited higher improvements in interfacial shear strength (IFSS) under tensile tests than compressive cases. However, ED treatment showed higher IFSS improvement than dipping case under both tensile and compressive test. The typical microfailure modes including fiber break, matrix cracking, and interlayer failure were observed during tensile test, whereas the diagonal slippage in fiber ends was observed during compressive test. For both the untreated and treated cases AE distributions were separated well under tensile testing. On the other hand, AE distributions were rather closer under compressive tests because of the difference in failure energies between tensile and compressive loading. Under both loading conditions, fiber breaks occurred around just before and after yielding point. Maximum AE voltage fur the waveform of carbon or basalt fiber breakage under tensile tests exhibited much larger than those under compressive tests.

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Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa

  • Kim, Sung Woo;Nho, Eun Jee;Lee, Joong Yeup;Jee, Byung Chul
    • Clinical and Experimental Reproductive Medicine
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    • v.46 no.4
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    • pp.147-151
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    • 2019
  • Objective: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). Methods: Frozen semen samples from 21 healthy donors were thawed and prepared by the swim-up technique for use in intracytoplasmic sperm injection. The semen samples were treated for 5 minutes as part of the HOST procedure and then underwent the sperm chromatin dispersion test using a Halosperm kit. DNA fragmentation status (large halo, medium halo, small halo, no halo, or degraded) and the specific tail swelling pattern ("a"-"g") were assessed at the level of a single spermatozoon. A total of 42,000 spermatozoa were analyzed, and the percentage of spermatozoa without DNA fragmentation (as evidenced by a large or medium halo) was assessed according to the specific tail swelling patterns observed. Results: The HOST examinations showed that > 93% of spermatozoa across all types displayed no DNA fragmentation. The percentage of spermatozoa without DNA fragmentation was 100% in type "d", 98.67% in type "g", and 98.17% in type "f" spermatozoa. Conclusion: We found that the type "d" spermatozoa displayed no DNA fragmentation, but the other types of spermatozoa also displayed very low rates of DNA fragmentation. This result may be associated with the processing of the spermatozoa by density gradient centrifugation and the swim-up technique.

Interfacial Evaluation and Microfailure Mechanisms of Carbon Fiber/Bismaleimide (BMI) Composites using Tensile/compressive Fragmentation Tests and Acoustic Emission (인장/압축 Fragmentation 시험법과 음향방출을 이용한 Carbon Fiber/Bismaleimide (BMI) Composites 의 계면 평가와 미세파괴 메커니즘 연구)

  • 김진원;박종만;윤동진
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2000.11a
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    • pp.79-83
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    • 2000
  • Interfacial and microfailure properties of carbon liber/bismaleimide (BMI) composites were evaluated using both tensile fragmentation and compressive Broutman tests with acoustic emission (AE). Since BMI is rather difficult matrix to apply for the conventional fragmentation test because of its too low elongation and too brittle and high modulus properties, dual matrix composite system was applied. After carbon fiber/BMI composite was prepared for rod shape by controlling differing curing stage, composites rod was embedded in toughened epoxy as outer matrix. The typical microfailure modes including fiber break, matrix cracking, and interlayer failure were observed during tensile testing, whereas the diagonal slippage in fiber ends was observed during compressive test. On the other hand, AE amplitudes of BMI matrix fracture were higher than carbon fiber tincture under tensile test because BMI matrix has very brittle and high modulus. The waveform of signals coming from BMI matrix fractures was consistent with AE amplitude result under tensile tests.

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A New Evaluation Method for Interfacial Properties of Composites using the Gradual Multi-Fiber Fragmentation Test (단계적 다섬유 Fragmentation 시험법을 이용한 복합재료의 계면적 특성에 대한 새로운 평가방법)

  • ;;Koichi Goda
    • Composites Research
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    • v.12 no.2
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    • pp.10-25
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    • 1999
  • A new evaluation method for the interfacial properties of fibrous composites based on a fragmentation technique is proposed by using the gradual multi-fiber composite, in which the inter-fiber spacing is gradually changed. The results showed that as the inter-fiber distance increased, the aspect ratio of broken fibers decreased while the interfacial shear strength between the fiber and matrix increased. When the reciprocal of the inter-fiber destance was taken for the above relations, both the aspect ratio and interfacial shear strength showed a saturated value. This means that the gradual multi-fiber composite indicates an upper bound in aspect ratio and an upper bound in interfacial shear strength. It was concluded that this fragmentation test could be a new method for composite evaluation, since reducing a difference between these two bounds is effective for composite strengthening. In addition an elastoplastic finite element analysis was carried out to relate the above results with fiber stress a distribution around fiber breaks. It was proved that the bound obtained in the gradual multi-fiber composite test is closely related to stress concentrations caused by a group of multi-fiber breaks.

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