• Title/Summary/Keyword: Free amino aicd

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Changes of Free Amino Acid and Free Sugar Contents in Barley Seedlings in Response to Anaerobic or Cold Treatment (혐기 또는 저온처리에 따른 보리 유식물체내 유리아미노산 및 유리산 함량의 변화)

  • 윤성중;유남희
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.2
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    • pp.139-144
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    • 1996
  • Effects of anaerobic, mechenical wounding and cold treatments on free amino acid and free sugar contents were examined in 7 day-old barley seedlings. In barley shoots under anaerobic condition, drastic changes in free amino acid content were observed. Alanine, ${\gamma}$-aminobutyric aicd(GABA) and glutamic acid contents changed most prominantly. Alanine and GABA contents increased rapidly with incubation time up to 10 hr. Glutamic acid content, however, decreased drastically during the first 5 hr of incubation, then increased thereafter. Barley shoots showed similar but smaller changes in amino aicd contents in response to mechanical wounding. GABA content increased in shoots and roots in response to the cold treatment. But increase in GABA content was greater in anaerobic than cold treatment. In anaerobically treated shoots, changes in free sugar content were less prominant than those in amino acid content. Sucrose content changed relatively larger than glucose and fructose contents in excised shoots.

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Comparison of Amino Acid by Appearance of Albinism in Cultured Flounder Paralichthys olivaceus (양식산 넙치의 백화현상에 따른 아미노산 조성의 비교)

  • 김종현
    • The Korean Journal of Food And Nutrition
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    • v.12 no.5
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    • pp.496-501
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    • 1999
  • Albinism is a phenomenon that color of the body surface is changed to white orfaint brown from the specific color to the species by difficiency of pigments due to mutation or disease. This study was undertaken to investigate the experimental basis on the appearance of albinism in cultured flounder Paralichthys olivaceus. The skin and muscle from the normal and albinic flounder were used by measuring contents of amino acid free amino acid. The results were summarized as follows: Contents of amino acid in theskin and muscle are different from normal and albinic flounder. Phenolic and sulfuric amino acids in the skin of normal flounder were 2 times those of albinic ones. Especially tyrosine contents of the skin in normal flounder were 24 times higher than those. Methionine was 26 times higher than those Phenylalanine was 1.6 times higher. In free amino aicd phosphoserine and phosphoethanolamine were a little higher than that ones. The melanin formation of the skin in flounder was affected by substrates such as phenolic amino acid and cofactor such as sulfuric amino acid.

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Changes in Taste Components of Marsh Calm Soup as Affected by Boiling Time (재첩국 제조시 가열시간에 따른 맛성분의 변화)

  • Chun, Soon-Sil;Suh, Jae-Soo;Oh, Chan
    • The Korean Journal of Food And Nutrition
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    • v.13 no.6
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    • pp.529-533
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    • 2000
  • The marsh calm soup was prepared with 2 volumes of boiling water and heated for 0, 5, 10, 30, 60 and 120 minutes. As the boiling time prolonged, water content decreased gradually, while protein content increased. Free amino acid content was the highest with 30 minutes of boiling showing 103.1 mg/100g, and decrease showly thereafter. Among the free amino acids, alanine was the most abundant followed by proline, glutamic acid and glycine. Degree of protein hydrolysis increased as boiling prolonged. In the non-volatile organic acids, succinic acid was the highest(137.08 mg/100g with 30 minutes of boiling), followed by oxalic acid, magic acid and lactic acid. The optimum boiling time for marsh calm soup was evaluated to be 30 minutes.

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The Correlation between the Gall-forming Process by Aphids and the Various Amino Acids in Zelkova Serrata Makino (느티나무에서 면충에 의한 충영형성과 아미노산종과의 연관성)

  • Uhm, Mi-Jeong;Oh, In-Suk;Kim, Hwan-Gyu;So, Sang-Sup
    • Korean Journal of Environmental Biology
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    • v.19 no.4
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    • pp.254-260
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    • 2001
  • After investigating the developmental process of galls forming on leaf of Zelkova serrata and analyzing the composition and contents of the free amino acids in leaves, galls and haemolymph of aphids, we have come to the following results. Galls induced were mostly distributed on the middle of lower part of leaf to the petiole along vein and most of investigated leaves had one gall only. The growth of galls continued in length and width until Paracolopha morrisoni habitants which transformed into alate adult left the gall. These results also indicate that galls are closely connected with a portion of plant tissues as well as foods and habitats of aphids. 26${\sim}$29 kinds of free amino acids such as proline, alanine and valine and so on, detected in leaves, gall and haemolymph of aphids. The composition of free amino acids were similar in three materials examined, but proline and asparatic acid showed the highest quantity in the leaves and falls, respectively. In case of aphids, alanine was extremely higher as 18.4% of total contents of free amino acids, in 1st instar of fundatrix stage. Total contents of free amino acids in the apteral adult stage of aphid were also increased than in the 1st instar stage. This investigation implies that nitrogen source such as free amino acids is apparently a major growth-material like carbohydrates during development of galls in Zelkova serrata.

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Comparison of Physicochemical Characteristics of Traditional and Commercial Kochujang during Fermentation (재래식과 공장산 고추장의 이화학적 특성 비교)

  • Kim, Young-Soo;Kwon, Dong-Jin;Oh, Hoon-Il;Kang, Tong-Sam
    • Korean Journal of Food Science and Technology
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    • v.26 no.1
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    • pp.12-17
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    • 1994
  • Physicochemical characteristics of traditional kochujang fermented for 6 months and commercial kochujang were compared. Tested kochujang included 18 kinds of Sunchang kochujang prepared with glutinous rice, 10 kinds of Boeun kochujang prepared with barley and 17 kinds of Sachun kochujang prepared with wheat, and 10 kinds of commercial kochujang. Major free sugar was found to be glucose both in traditional and commercial kochujang. Fructose, maltose, and sucrose were also detected in small amounts. The contents of free sugars in traditional kochujang was approximately one fourth of those presented in commercial kochujang. Commercial kochujang showed the highest level of total free amino acids followed in decreasing order by Sachun, Sunchang, and Boeun kochujang. The most abundant free amino acid was serine in Sunchang kochujang and aspartic acid both in Boeun and Sachun kochujang. On the other hand, glutamic acid was the most abundant amino acid in commercial kochujang. Volatile organic acids in various kochujang were determined and acetic, propionic, butyric, and 3-methyl butanoic acids were found in traditional kochujang. However, 3-methyl butanoic arid was not found in commercial kochujang. The most abundant volatile acid was acetic acid in both tradtional and commercial kochujang. Analysis of non-volatile organic acids showed that large amounts of lactic, oxalic, and succinic acids were found in traditional and commercial kochujang. In addition to these, small amounts of itaconic, malic, malonic, and pyroglutamic acids were found in commercial kochujang.

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Changes in Composition during Aging of Traditional Andong Sickhae (전통 안동식혜의 숙성과정중 성분변화)

  • Choi, Cheong;Lim, Seong-Il;Seog, Ho-Moon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.381-387
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    • 1991
  • Andong Sickhae is a traditional Korean fermented rice product which is made from glutionous rice, male, radish, ginger and red pepper. The changes in chemical composition, pH, amino nitrogen, amino acid, enzyme activity and free sugar of a traditional Andong Sickhae were monitored during the fermentation and storage at $4^{\circ}C$. The changes in ash, crude fat and moisture the contents during Andong Sickhae fermentation and storage were negligible. The pH of the product tended to decrease in the course of fermentation and storage and it showed the minimum value of 3.90 after 20th day of storage. On the other hand the maltose continued to increase up from 6.35g to 9.85g/100ml by 15th day of storage. The content of amino nitrogen in Andong Sickhae gradualy increase up to 22.40mg% by 3th day of fermentation. Glutamic aicd and aspartic acid were the major amino acid in water and salt soluble protein in Andong Sickhae.

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Changes in Physicochemical Components of Stewed Pumpkin Juice Heated and Stored under Different Conditions (가열 및 저장조건에 따른 호박즙의 이화학적 성분변화)

  • 박복희;김현아;박영희;오봉윤
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.1
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    • pp.1-9
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    • 1998
  • This study was designed to determine the most suitable manufacturing conditions of stewed pumpkin juice(SPJ) in terms of nutrition and taste. Well ripened pumpkin was heated for 3, 4, 5, 6 and 7 hours in a pressure cooker and then extracted and packed in retort pouches. These were kept at 4$^{\circ}C$ and 28$^{\circ}C$ for 30 days, respectively and changes of the physicochemical components of SPJ were investigated. The free amino acid compositions of pumpkin and SPJ occupied asparagine, aspartic acid, glutamine, arginine and serine in increasing order of abundance. Except SPJ heated for 3 hours, SPJ stored at 28$^{\circ}C$ had the most abundant free amino acids, followed by SPJ stored at 4$^{\circ}C$, and SPJ not stored. The fatty acid composition of pumpkin occupied behenic acid, erucic acid, palmitic acid, linoleic acid, heneicosanoic aicd, in order of abundance. When SPJ was heated over 3 hours, palmitic acid and linolenic acid decreased greatly. SPJ stored at 4$^{\circ}C$ and 28$^{\circ}C$ increased contents of saturated fatty acids such as behenic acid, palmitic acid than SPJ not stored. As SPJ was heated for a long time, carotenoid was mostly destroyed and the Hunter "L", "a" and "b" values of SPJ decreased. Among the mineral constituents of SPJ, Na and Ca were dominantly occupied. It was found that the range of the total visible cell count was 3~4CFU/ml from the SPJ which was heated for 3 hours and it increased by 1$\times$109CFU/ml when the SPJ was stored at 28$^{\circ}C$. It seems that the contamination by microorganism have occurred at packing process. In sensory evaluation, the SPJ which was heated for 5 hours had the highest scores in overall preference, sweet smell and sweetness.ce, sweet smell and sweetness.

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Studies on Components Related to Taste such as Free Amino Acids and Nucleotides in Korean Native Chicken Meat (한국 토종 닭고기의 정미 성분 중 유리아미노산과 핵산관련물질에 대한 연구)

  • Ahn, Dong-Hyun;Park, So-Youn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.4
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    • pp.547-552
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    • 2002
  • Characteristics of components related to meat taste of broiler and Korean native chicken (KNC) were investigated. Carnosine was detected much more in the breast meat of KNC at 28 weeks of age and in the leg meat of KNC over 6 weeks of age than broiler. Glutamic acid was much contained in the leg meat of KNC over 11 weeks of age. In the case of breast meat, the content of glutamic acid was higher in KNC over 6 weeks of age than that of others. The ratio of good-tasting amino acid to bitter-tasting amino acid was higher in KNC over 11 weeks of age than broiler. Concentration of IMP in KNC over 15 weeks of age was higher than that of broiler.

Preparation and Characteristics of Functional Sauce from Shrimp Byproducts (새우 부산물을 이용한 기능성 소스의 제조)

  • Heu, Min-Soo;Kang, Kyung-Tae;Kim, Hye-Suk;Yeum, Dong-Min;Lee, Tae-Gee;Park, Tae-Bong;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.2
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    • pp.209-215
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    • 2007
  • The functional sauce from shrimp byproducts (heads, shells and tails) was prepared and examined for its characterization. The results of volatile basic nitrogen (VBN) suggested that shrimp byproducts were suitable materials for preparing functional sauce. The shrimp hydrolysate, which was incubated with Alcalase for 30 min, showed excellent yield and ACE inhibitory activity. The concentrated sauce from shrimp byproduct was high in crude protein, while low in VBN content and salinity when compared to commercial shrimp sauce. The total amino acid content (23,095.2 mg/100 mL) of concentrated sauce from shrimp byproduct was higher than that (4,582.5 mg/100 smL) of commercial shrimp sauce; also, the major amino acids were glutamic acid, aspartic acid, arginine and lysine. The free amino acid content and taste value of concentrated sauce from shrimp byproduct were 2,705.5 mg/100 mL and 81.0, respectively. The results on the taste value of concentrated sauce from shrimp byproducts suggested that the major taste active compounds among free amino acids were glutamic acid and aspartic acid.

Effect of Processing Methods on the Chemical Composition of Panax ginseng Leaf Tea (고려인삼엽차의 제조방법에 따른 화학성분 조성)

  • Chang Hyun-Ki
    • The Korean Journal of Food And Nutrition
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    • v.18 no.1
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    • pp.63-71
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    • 2005
  • Panax ginseng leaves are produced as the by-product when Panax ginseng roots were harvested. The Panax ginseng leaves was examed for the applicable possibility as the functional food. In this study, the changes in chemical composition of Panax ginseng leaves was examed by three methods as the hot-air dried(DRT), the aged tea(AGT) and the heat processed tea(HPT). The general composition of Panax ginseng leaves tea was shown as similar results in 3 different process methods. The level of the crude lipid and reducing sugar concentration were decreased slightly in HPT. The free sugar content of DRT was higher than the HPT and AGT. The existence of the higher content of free sugar composition in order are sucrose, fructose and glucose. The concentration of serine was the highest in the free amino acids, which were shown from 309.6 mg% to 336.6 mg%. The contents of free amino acid in Panax ginseng leaves made by DRT was higher than by AGT and HPT. The concentration of Ca was shown as the highest content among the minerals and was 2,115 mg%. The contents of minerals were existed in order of Ca, K, Mg, P, Na, Mn, Fe, Zn and Cu. But there were hardly any remarkable differences of mineral concentrations of Panax ginseng leaves tea made by different processing methods. The concentration of water soluble solid of Panax ginseng leaves tea processed by HPT was higher than by DRT and AGT. The concentration of ascorbic acid was shown the highest value of 424.4mg% in HPT. There was no differences in the fatty acid composition according to their processing methods. The concentration of palmitic acid was higher than that of other fatty acid. The order of fatty acid concentration were palmitic aicd, linoleic acid, linolenic acid, oleic acid and stearic acid, abundantly. As a conclusion, HPT was shown as the best process method for the production of Panax ginseng leaves tea.