• Journal of Food Hygiene and Safety
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• v.29 no.1
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• pp.67-72
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• 2014

This study investigated the antibacterial effects of GR ethanol extracts (GRE), sodium chlorate (SC) and a combination of GRE and SC (GS) on Brucella abortus (B. abortus). The antibacterial activities of GRE, SC and GS towards B. abortus were evaluated by incubating B. abortus with GRE, SC and GS. Following treatment with GRE, SC and GS, B. abortus survival and intracellular proliferation in macrophages were monitored. In the cellular cytotoxicity assay, GRE, SC and GS are not cytotoxic at concentrations less than $400{\mu}g/ml$, 15 mM and 0.6GS (1 of GS, GRE $1,000{\mu}g/ml$ + SC 30 mM), respectively. The viability of B. abortus was markedly decreased in a dose-dependent manner in all treatment groups. In addition, B. abortus intracellular proliferation within macrophages was significantly reduced in cells treated with GRE ($400{\mu}g/mL$), SC (15 mM) and 0.5GS (GRE $500{\mu}g/mL$ + SC 15 mM) after 48 hr-incubation (GRE, p < 0.01; SC and 0.5GS, p < 0.001). Especially, in the treatment of GS, the synergistic effect of GRE and SC treatment on B. abortus in macrophage was observed. In conclusion, GS is useful as an antibacterial candidate against B. abortus, and can be applied in the field of meat and milk hygiene.

• KIISE Transactions on Computing Practices
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• v.23 no.3
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• pp.171-176
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• 2017

Recently, several information-technology research projects such as those for high-performance computing, the cloud service, and the DevOps methodology have been advanced to develop the efficiency of satellite data-processing systems. In March 2019, the Geostationary Ocean Color Imager II (GOCI-II) will be launched for its predictive capability regarding marine disasters and the management of the fishery environment; moreover, the GOCI-II Ground Segment (G2GS) system for data acquisition/processing/storing/distribution is being designed at the Korea Ocean Satellite Center (KOSC). The G2GS is composed of the following six functional subsystems: data-acquisition subsystem (DAS), data-correction subsystem (DCS), precision-correction subsystem (PCS), ocean data-processing subsystem (ODPS), data-management subsystem (DMS), and operation and quality management subsystem (OQMS). The G2GS will enable the real-time support of the GOCI-II ocean-color data for government-related organizations and public users.

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.999-1010
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• 2016

Ganoderma lucidum BCCM 31549 has a long established role for its therapeutic activities. In this context, much interest has focused on the possible functions of the (1,3)-β-D-glucan (G) produced by these cultures in a stirred-tank bioreactor and extracted from their underutilized mycelium. In the existing study, we report on the systematic production of G, and its sulfated derivative (GS). The aim of this study was to investigate G and its GS from G. lucidum in terms of their antibacterial properties and cytotoxicity spectrum against human prostate cells (PN2TA) and human caucasian histiocytic lymphoma cells (U937). ¹H NMR for both G and GS compounds showed β-glycosidic linkages and structural similarities when compared with two standards (laminarin and fucoidan). The existence of characteristic absorptions at 1,170 and 867 cm^-1 in the FTIR (Fourier Transform Infrared Spectroscopy) for GS demonstrated the successful sulfation of G. Only GS exhibited antimicrobial activity against a varied range of test bacteria of relevance to foodstuffs and human health. Moreover, both G and GS did not show any cytotoxic effects on PN2TA cells, thus helping demonstrate the safety of these polymers. Moreover, GS showed 40% antiproliferation against cancerous U937 cells at the low concentration (60 μg/ ml) applied in this study compared with G (10%). Together, this demonstrates that sulfation clearly improved the solubility and therapeutic activities of G. The water-soluble GS demonstrates the potential multifunctional effects of these materials in foodstuffs.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.409-417
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• 2018

Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the isolated Bacillus strains were further screened by morphological, biochemical, and genomic analyses. The screened strains were then used to test the utilization of the most common carbon (glucose, xylose, fructose, sucrose) and nitrogen (yeast extract, corn steep liquor) sources for the economical production of 2,3-BDO. Two-stage fed-batch fermentation was finally carried out to enhance 2,3-BDO production. In consequence, a newly isolated Bacillus licheniformis GSC3102 strain produced 92.0 g/l of total 2,3-BDO with an overall productivity and yield of 1.40 g/l/h and 0.423 g/g glucose, respectively, using a cheap and abundant nitrogen source. These results strongly suggest that B. licheniformis, which is found widely in nature, can be used as a host strain for the industrial fermentative production of 2,3-BDO.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1302-1313
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• 2021

In this study, Gami-Sumiwon (GS; Cynanchi wilfordii R., Angelica gigantis R., Lycium chinense M., Betula platyphylla S., Cryptotympana atrata F. and Carthamus tinctorius L.) and/or Sinomenium acutum R. (SC) was extracted with 70% ethanol or water. And We investigated the antioxidant activity effect of GS±SC. The following experimental techniques were used to evaluate the antioxidant efficacy of GS±SC. HPLC chromatogram, heavy metal content, ABTS/DPPH radical scavenging analysis, SOD-like activity assay, FACS, and NO assay. As a result of the experiment, the sinomenine content was found to be higher in DW extracts, and decursin was found to be higher in 80% ethanol extracts. And, the amount of heavy metals in all extracts was below the standard value. ABTS, and DPPH radical scavenging activity was identified that GS±SC(EtOH) was found to have a higher scavenging activity than GS±SC(DW). But, SOD showed the opposite result. No cytotoxicity of GS was observed on Raw 264.7 cells at concentration of 1~100 ㎍/㎖. The ROS production was significantly decreased that GS±SC(DW) was found to have a higher scavenging activity than GS±SC(EtOH). However, NO production showed the opposite result. Looking at the results of SOD and ROS analysis, SC does not seem to have a function of prevention. SC is thought to have an effect on the removal of free radicals generated after oxidative stress. This result objectively confirmed the antioxidant effect of GS±SCs. We will continue to conduct in-depth research. Therefore, it is believed that the possibility of using GS±SCs as a functional material can be established. The more diverse the objectives, the higher the value of GS utilization is thought to be.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.44-44
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• 1993

Rat 기관 평활근에서 GS 283은 Carbachol에 의한 수축을 용량 의존적으로 억제시켰으며 특히 $Ca^{2＋}$-free 용액에서 G5 283은 농도에 비례하여 $Ca^{2＋}$에 의한 수축을 억제 시켰으며, 전형적인 $Ca^{2＋}$ 길항제인 Verapamil도 유사한 효과를 나타내었으나 GS 283보다 강력하였다. GS 283은 칼슘 길항 효과 이외에도 약하지만 Antihistamine 및 Antimuscarine작용도 가지고 있는 것으로 나타났다. 한편 Rat 대동맥에서 GS 389는 Phenylephrine(PE)에 의한 수축을 용량 의존적으로 억제시켰으며 $Ca^{2＋}$-free 용액에서 PE에 의한 수축과 KCI에 의한 수축율 모두 억제시켰다. 이러한 결과는 GS283과 GS 389는 $Ca^{2＋}$에 길항적용이 있음을 암시하며 특히 수용체를 통한 $Ca^{2＋}$-channel과 세포막의 voltage를 통한 $Ca^{2＋}$-channel을 모두 억제함을 강력히 시사한다. 향후 in vivo model에 대한 이들 약물의 효과를 연구해 보아야 할 것으로 생각하며 이러한 효과가 직접 $Ca^{2＋}$-channel에 대한 작용인지 또는 GS화합물이 cyclic nucleotide를 증가시키는 효과가 있어서 이로 인한 2차적 작용인지를 더욱 밝혀야 할것으로 생각된다.

• Journal of the Korea Society of Computer and Information
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• v.24 no.2
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• pp.49-56
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• 2019

In this paper, we propose an efficient congestion control scheme for interworking between 5GS(5G system) and LTE(Long-Term Evolution), called ECC(Efficient Congestion Control). The proposed congestion control scheme (ECC) is considered for coexistence of 5GS and legacy LTE systems and provides a prompt service connectivity based on overriding method while the backoff timer is running in the UE. Also, we briefly introduce Rel-15 5GS from a congestion control perspective and the proposed ECC and simulation results for the existing legacy congestion control mechanism and ECC in the 5GS-LTE coexisting environment are presented. Lastly, the improvement direction is considered in future 3GPP 5GS phase 2 standard in this paper.

• Biomolecules & Therapeutics
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• v.25 no.5
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• pp.497-503
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• 2017

Recent reports claimed that glucosylsphingosine (GS) is highly accumulated and specifically evoking itch-scratch responses in the skins of atopic dermatitis (AD) patients. However, it was unclear how GS can trigger itch-scratch responses, since there were no known molecular singling pathways revealed yet. In the present study, it was verified for the first time that GS can activate mouse serotonin receptor 2a (mHtr2a) and 2b (mHtr2b), but not 2c (mHtr2c) that are expressed in HEK293T cells. Specifically, effects of GS on all mouse serotonin receptor 2 subfamily were evaluated by calcium imaging techniques. The GS-induced intracellular calcium increase was dose-dependent, and antagonists such as ketanserin (Htr2a antagonist) and RS-127445 (Htr2b antagonist) significantly blocked the GS-induced responses. Moreover, the proposed GS-induced responses appear to be mediated by phospholipase C (PLC), since pretreatment of a PLC inhibitor U-73122 abolished the GS-induced responses. Additionally, the GS-induced calcium influx is probably mediated by endogenous TRPC ion channels in HEK293T cells, since pretreatment of SKF-96365, an inhibitor for TRPC, significantly suppressed GS-induced response. In conclusion, the present study revealed for the first time that GS can stimulate mHtr2a and mHtr2b to induce calcium influx, by utilizing PLC-dependent pathway afterwards. Considering that GS is regarded as a pruritogen in AD, the present study implicates a novel GS-induced itch signaling pathway.

• Journal of KIISE:Computer Systems and Theory
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• v.28 no.1_2
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• pp.73-79
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• 2001

단순 다각형 P에 대한 새로운 화량문제인 경비가능충분집합(Guard Sufficiency Set:GSS)에 대하여 소개하고 정의하였다. 집합 S P가 P의 경비가능집합이라는 의미는 P의 각 점들이 최소한 S에 있는 한 점으로부터 보인다는 것을 의미한다. 집합 G P가 P의 경비가능충분집합이라는 의미는 G를 경비 가능한 임의의 집합 S P가 P 역시 경비 가능하다는 것을 의미한다. 본 논문에서는 꼭지점에 대한 GSS가 다각형 전체에 대한 GSS가 되는 다각형 부류를 제시하고, 또한 에지에 대한 GSS가 다각형 전체에 대한 GSS가 되는 다각형 부류를 제시한다. 그리고, P의 꼭지점에 대한 GSS의 크기에 대한 하한과 상한을 제시하고, 기하학적 요소에 특정 제약조건을 주어 대체 GSS을 정의할 수 있음을 보인다. 이 외에도 다양한 GSS 문제들을 소개하고, 기하학적 요소에 제약을 가한 대체 GSS를 정의하고 그와 관련된 가설을 하나 제시한다.

• Microbiology and Biotechnology Letters
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• v.28 no.3
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• pp.139-146
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• 2000

산업적으로 유용한 미생물 유래 셀룰로오스를 생산 이용하기 위해 전국 각지 양조식초의 덧으로부터 세룰로오스의 생산성이 높고 조질의 균일성을 나타내는 균주를 분리하였다 분리균 GS11은 gram 음성이고 간균(0.6$\times$2.2~3.2 $\mu\textrm{m}$)의형태를 하고 있으며 편모를 가지고 있어 운동성을 보였다. 또한 세포내 지방산 조성은 다량의 불포화 지방산 {{{{ {C}_{18:1} }}}}과 포화지반산 {{{{ {C}_{16:0 } }}}}, {{{{ {C}_{14:0 } }}}} 이 대부분을 차지하였고 DNA 염기조성 (G+C) 함량은 58.4% 이였으며 ubiqunone 은 {{{{ { Q}_{10 } }}}}을 갖는 것으로 나타났다 이러한 형태학적 생리.생화학적 특성의 결과에 따라 본 균주는 Acetobacter xylinum GS11으로 동정되었다 A. xylinum, GS11 의배양기간 동안 셀룰로오스 생산성을 검토하고자 250mL 삼각플라스크에 균주를 접종하여 $30^{\circ}C$에서 12일간 정치배양하였다 그결과 기질인 glucose의 소비는 접종 후 급소하게 감소하여 셀룰로오스 생산에 이용되었으며 셀룰오스의 생산은 배양 9일 경에 2.8g/l로 최대의 생산량을 나타냈다.