• Journal of Aquaculture
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• v.18 no.4
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• pp.252-259
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• 2005

Isozyme and AFLP analyses were examined to estimate the utilities of them as a genetic marker. The utilities were evaluated by genetic divergence and relationships among the four distinct abalone species; Haliotis discus hannai collected from northeast coast of Japan and Yellow-Sea coast of China, H. rufescens collected from west coast of USA, H rubra collected from southeast coast of Australia and H midae collected from Cape Town of South Africa. Isozyme and AFLP analyses showed a clear genetic divergence between every pair of species. Genetic relationships among the low species estimated by isozyme and AFLP analyses reflected to geographical distribution and morphological characteristics. In conclusion, Isozyme and AFLP analyses are suitable genetic markers far estimates of genetic divergence and relationship among abalone species.

• Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.101-106
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• 2006

The cytochrome c oxidase subunit I (COI) gene region of mitochondrial DNA (mtDNA) was examined in four abalone species to estimate its utility as a genetic marker using restriction fragment length polymorphism (RFLP) analysis. The utility was evaluated in terms of genetic divergence and relationships among Haliotis discus hannai, H. rufescens, H. rubra, and H. midae in both hemispheres of the world. There was clear genetic divergence in the mtDNA COI region between all pairs of the four species. Moreover, relationships among the abalone species were reflected in their geographical distributions and morphological characteristics. Therefore, RFLP analysis of the mtDNA COI region is a suitable genetic marker for the estimation of genetic divergence and relationships among abalone species. However, it is not effective for the evaluation of genetic differences within abalone species.

• Journal of Aquaculture
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• v.21 no.4
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• pp.346-350
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• 2008

RAPD analysis was examined to estimate the availability as a genetic marker. The availability was evaluated in terms of genetic divergence and relationships among Haliotis discus hannai, H. rufescens, H. rubra and H. midae in both hemispheres of the world. In results, RAPD analysis showed a clear genetic divergence between every pair of species. However, genetic relationships among the four species estimated by RAPD analysis unreflected to geographical distribution and morphological characteristics. In conclusion, RAPD is suitable genetic markers for estimates of genetic divergence and differences among abalone species.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1238-1243
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• 2008

Domestic buffalo and cattle are two extremely important livestock species in worldwide agricultural production. In this paper, to investigate genetic diversity and divergence among swamp buffalo, river buffalo and cattle, 30 microsatellite markers were screened on 168 individuals sampled from five populations. Substantial differences were observed among the three groups of animals with respect to allele frequency distribution, allele size and polymorphism. The cattle sample (Mongolian) showed significantly higher genetic variability (0.674 of gene diversity, p<0.01), and the swamp and river buffalo samples displayed similar degree of genetic variation (0.536 in swamp and 0.546 in river, p = 0.92). Results of both phylogenetic tree and multivariate analysis could distinguish three groups of animals, suggesting their deep evolutionary divergence. Additionally, using $({\delta}{\mu})^2$ genetic distance, we estimated a divergence time of 1.7 million years between swamp and river buffalo that strongly supported distinct genetic origins for the two buffalo types.

• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.63-68
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• 2008

The induced genetic divergence was estimated in 44 mutant lines of finger millet variety GPU 26, developed by single and combination treatments with gamma rays, EMS and NG using three multivariate analyses. The mutant lines were grouped into eight genetically diverse clusters by multivariate D2 and canonical analyses and 11 clusters by dendrogram grouping through Gower's similarity coefficient. The clustering pattern in these three methods was almost similar. Twelve mutant lines in D2 and 13 in the dendrogram grouping method were grouped in the parental cluster(Cluster I) indicating that they did not possess enough divergence from the parent to be classified as micromutant lines. However a large proportion of mutant lines showed divergence from the parent variety and also among themselves. No definite relationship of mutagenic origin and clustering of mutant lines were observed. The mutant lines developed from the same mutagenic treatments often grouped into different clusters indicating that each mutagenic treatment was effective in inducing diverse types of changes in the nine traits studied. The hybridization program between the divergent mutant lines GE 2-2 or GE 3-4 with GG 3-1 is expected to give promising and desirable segregants in subsequent generations. Traits such as days to 50% flowering and days to maturity had major contributions to the induced genetic divergence.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.79-81
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• 2001

A study was conducted to establish the degree of genetic divergence between different hybrid forms and rearing conditions through estimation of the minimum number of genes (allelic pairs) differentiating parents in terms of specific quantitative traits. It was established that the minimum gene numbers differentiating parental lines in the inheritance of cocoon was 1, of cocoon shell weight- between 1 and 2, and of silk filament length- between 2 and 3. The variability in the specific genetic parameter could be explained by the reliability of the statistical-and-genetic method used and the expression of genes affecting the formation of each of the characters tested. Gene expression, in its turns is conditioned both by the gene interaction within the genotypes and the different genotype response to environmental change. To go deep in the problem, experiments should be conducted under strictly controlled conditions, reducing the mathematical-and-genetic analysis to a physiological levels and hence to analyse the genetic nature of the specific quantitative character formation and its genetic control.

• International Journal of Industrial Entomology and Biomaterials
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• v.24 no.1
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• pp.41-47
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• 2012

The Scarites aterrimus (Coleoptera: Carabidae) dwells exclusively on coastal sandy dunes. Previously, we investigated the nation-wide magnitude and nature of genetic diversity of the species using mitochondrial COI gene and found moderate to low magnitude of sequence diversity, the presence of closely related haplotypes, and relatively high gene flow estimate. Based on these observations we concluded that the species had no historical barriers that bolster genetic subdivision and possible population decline. In this study, we additionally sequenced mitochondrial COII gene from 23 individuals collected from 9 Korean localities to confirm previous findings. Sequencing of 688 bp COII gene provided 5 haplotypes ranging in sequence divergence from 0.145% to 0.291% (1 ~ 2 bp), further confirming low sequence divergence of the species. Gene flow estimates and genetic diversity estimates also support the previous findings that there had been no historical barriers that bolster genetic subdivision.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.3
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• pp.315-319
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• 2003

Random amplified polymorphic DNA (RAPD) analysis was done with 19 oligonucleotide primers to study genetic similarities and divergence among different types of Deoni breed of cattle viz., Balankya, Wannera and Waghya. Six random primers produced low to high numbers of polymorphic bands between pooled DNA of different Deoni types. Of the 48 RAPD markers obtained 33 were common to all Deoni types, 3 were individual specific and 12 were polymorphic for different Deoni types. The mean average percentage difference values among Deoni types showed that Balankya and Wannera had less genetic divergence when compared to Waghya.

• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.773-782
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• 1996

Sixty wild rate (fifty eight of Rattus norvegicus and two R rattus) were caught from Seoul, Kyonggi, Kangwon, Honam, and Yongnam areas between August and October 1992. From liver homogenates of the wild rats, isoenzyme patterns were analysed by starch gel electrophoresis. Using 9 enzyme systems, eight electrophoretic types were identified among wild rats with genetic diversity per locus between 0.00 and 0.49 (Mean 0.15). R rattus from Kyonggi (Kanghwa) and Kangwon (Cholwon) were distinct from R norvegicus from nine regions with 0.581 in genetic divergence. Therefore genetic divergence was different not only in interspecies(0.581) but also in intraspecies(0.111~0.375). These data suggested that isoenzyme electrophoresis could be used as a potential application in taxonomic studies of wild rats.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.6
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• pp.699-707
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• 1995

Eurasian yellow perch (Perca fluviatilis) and American yellow perch (Perca flavescens) are known to be endemic species in Eurasia and North America, respectively. The presence of endemic species on each continent suggests their independent evolutionary history. However, because of the morphological similarity, distribution pattern, and only recent fossil record, their divergence time and speciation of the two Perca species has long been controversial. Here, from the comparison of the entire nucleotide sequences of cytochrome b gene, large genetic divergence between the two Perca species is observed although they are morphologically similar each other. Among 1,140 base pairs, interspecific nucleotide differences are found at 130 sites $(11.4\%)$. The differences varies with codon position, showing 22 sites in the first, 5 sites in the second, and 103 sites in the third codon position. Considering the types of nucleotide changes, transitional differences are much more than transversional differences and its ratio turned out to be 5.19. The estimated divergence time of the two Perca species indicates that they were separated each other approximately in the late Miocene period, which implies the long history of speciation. With comparison of the inferred amino acid sequences, strong structural and functional constraints which seem to be maintained by the highly conservative amino acid residues or protein regions, as found in other taxonomic groups of organisms, are also recognized in the cytochrome b of the fishes examined.