• Title/Summary/Keyword: Genetic variation

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Genetic Analysis of Asian Chum Salmon Populations Based on Microsatellite DNA Variation

  • Yoon, Moon-Geun;Abe, Syuiti;Jin, Deuk-Hee
    • Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.186-190
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    • 2007
  • We examined the genetic variability of Asian chum salmon (Oncorhynchus keta) populations using nuclear microsatellite (ms) DNA analysis with four polymorphic loci (OKM4, OKM5, OKM7, and OKM8) in 397 individuals from nine populations, including one in Korea, seven in Japan, and one in Russia. The msDNA gene diversity was highest in the Japanese populations, suggesting greater genetic variation in the populations in Japan than in populations in Korea and Russia. The pairwise $F_{ST}$ estimates based on our msDNA data showed that the Korean population was genetically different from the Japanese and Russian populations, and there were higher $F_{ST}$ estimates between Hokkaido and Honshu populations than between other population pairs. A neighbor-joining tree showed that the Korean population was distinct from two other clusters, representing the populations in Honshu and the populations in Hokkaido and Russia. These results suggest that the observed population genetic patterns of Asian chum salmon might be influenced by low or restricted gene flow.

Polymorphism Assessment of Six Lentil (Lens culinaris Medik.) Genotypes Using Isozyme

  • Madina, M. Hur;Rahman, M. Saifur;Deb, A. Chandra;Choi, Yun Hee;Kim, Mi Ri;Shin, Jihoon;Yoo, Jin Cheol
    • Journal of Integrative Natural Science
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    • v.8 no.2
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    • pp.117-127
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    • 2015
  • Lentil (Lens culinaris Medik.) is one of the important legumes and cheaper source of protein in Bangladesh that displays great biological diversity. Isozyme, one of the most important protein markers to detect genetic polymorphism in lentil, whereas we considered thirteen-isozyme in six varieties viz., BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. The highest polymorphism was found in tyrosinase isozyme system. UPGMA analysis revealed that the highest similarity between BARI masur-5 and BARI masur-6 whereas, the highest genetic distance between BARI masur-1 and BARI masur-5 reflecting higher intervarietal variation. Principal component analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 81.58%, 11.19% and 4.94% variation respectively, with cumulative variation of the first three PCs was 75.45%. Consequently, Isozyme could clearly assed the genetic diversity at intervarietal levels and these two varieties can be considered as valuable gene resources for future breeding and conservation programs.

Genetic Relationships among Korean Adlay, Coix lachryma-jobi L., Landraces Based on AFLPs

  • Moon Jung-Hun;Jang Jung Hee;Park Jung Soo;Kim Sung Kee;Lee Kyung-Jun;Lee Sang-Kyu;Kim Kyung-Hee;Lee Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.2
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    • pp.142-146
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    • 2005
  • Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.

Genetic Variation and Biological Control of Fusarium graminearum Isolated from Wheat in Assiut-Egypt

  • Mahmoud, Amer F.
    • The Plant Pathology Journal
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    • v.32 no.2
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    • pp.145-156
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    • 2016
  • Fusarium graminearum Schwabe causes Fusarium head blight (FHB), a devastating disease that leads to extensive yield and quality loss of wheat and other cereal crops. Twelve isolates of F. graminearum were collected from naturally infected spikes of wheat from Assiut Egypt. These isolates were compared using SRAP. The results indicated distinct genetic groups exist within F. graminearum, and demonstrated that these groups have different biological properties, especially with respect to their pathogenicity on wheat. There were biologically significant differences between the groups; with group (B) isolates being more aggressive towards wheat than groups (A) and (C). Furthermore, Trichoderma harzianum (Rifai) and Bacillus subtilis (Ehrenberg) which isolated from wheat kernels were screened for antagonistic activity against F. graminearum. They significantly reduced the growth of F. graminearum colonies in culture. In order to gain insight into biological control effect in situ, highly antagonistic isolates of T. harzianum and B. subtilis were selected, based on their in vitro effectiveness, for greenhouse test. It was revealed that T. harzianum and B. subtilis significantly reduced FHB severity. The obtained results indicated that T. harzianum and B. subtilis are very effective biocontrol agents that offer potential benefit in FHB and should be harnessed for further biocontrol applications. The accurate analysis of genetic variation and studies of population structures have significant implications for understanding the genetic traits and disease control programs in wheat. This is the first known report of the distribution and genetic variation of F. graminearum on wheat spikes in Assiut Egypt.

Genetic variation and differentiation among populations of Euphorbia ebracteolata (Euphorbiaceae) in Korea (한국산 붉은대극(대극과) 집단의 유전적 변이와 분화)

  • Cho, Young-Woo;Park, Ki-Ryong
    • Korean Journal of Plant Taxonomy
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    • v.45 no.1
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    • pp.1-7
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    • 2015
  • Using starch gel electrophoresis, ten isozyme loci were examined in nine populations of Euphorbia ebracteolata in Korea. Populations of E. ebracteolata tend to have higher within-population levels of genetic variation (A = 2.2, P = 61.1, He = 0.165) along with low levels of genetic differentiation ($F_{ST}$ = 0.075) compared to previously reported Euphorbia species. The relatively high genetic variation in E. ebracteolata may be due to its long-lived regional distribution and to the outcrossed nature of the species. Low levels of genetic differentiation and the absence of unique alleles in E. ebracteolata populations suggest that Korean populations diverged recently. This study does not support the recognition of var. coreana and for. magna as separate taxa, and suggests that they should be combined with E. ebracteolata.

Genetic Variation in the Natural Populations of Abies holophylla Max. Based on RAPD Analysis (RAPD 분석(分析)에 의한 전나무 천연집단(天然集團)의 유전변이(遺傳變異))

  • Kim, In Sik;Hyun, Jung Oh
    • Journal of Korean Society of Forest Science
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    • v.88 no.3
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    • pp.408-418
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    • 1999
  • On the basin of RAPD analysis, genetic diversity and structure of the natural populations of Abies holophylla was estimated by AMOVA procedure. The average value of percent of polymorphic markers was 71.9%. Most variation existed among individuals within population(80.2%). Genetic differentiation among populations(${\Phi}_{ST}$) was 0.198. When the populations were grouped as two region(i.e., Taebaek and Sobaek Mountain Regions), 8.5% of the total genetic variation was explained as regional differences. The heterogeneity of molecular variance among populations was investigated with Bartlett's test, which revealed that populations of Mt. Taebaek and Mt. Gariwang were more heterogeneous. Generally, the populations of Taebaek Mountain Reion were more heterogeneous than those of Sobaek Mountain Reion. Finally, the applicability of AMOVA to the populations frenetic study was discussed in comparison with other measures of genetic differentiation which were widely used.

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Variation in Flower Color among Hybrids of Jeoktanshim Hibiscus syriacus L

  • Park Hyung-Soon;Cho Yoon-Jin;Chung Hun-Gwan;Jang Yong-Suk;Chung Dong-Jun
    • Plant Resources
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    • v.8 no.3
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    • pp.250-257
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    • 2005
  • This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the flowers and leaves, and genetic variation of the flowers color of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean flower height and width were 5.35 cm and 7.69 cm, respectively. Mean length and width of petal were 5.43 cm and 3.80 cm respectively, and mean length and width of flower pistil were 4.67 cm and 0.44 cm, respectively. The flower color of all ten individuals was the color of the Jeoktanshim-line, and Pyungsung, and all flower type were I-c type.

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Decrease in Genetic Variation of Overwintering Populations of the Diamondback Moth during Seasonal Occurrence (배추좀나방의 계절적 발생과 월동집단의 유전적 분화 감소)

  • Kim, Eunseong;Park, Areum;Park, Youngjin;Kim, Jooil;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.303-310
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    • 2015
  • The diamondback moth, Plutella xylostella, overwinters in some protected areas in Korea. Using a sex pheromone trap, the adults were monitored since the occurrence of the overwintering populations. In Andong, P. xylostella exhibited four adult peaks in a year. Biological characters, such as cold tolerance, insecticide susceptibility, and developmental rate, were analyzed and showed a significant variation among different local overwintering populations. Population genetic variation was assessed with molecular markers, in which the initial high genetic variation among the overwintering populations decreased with the progress of seasons. These results suggests that there may be a significant migration of P. xylostella to decrease the genetic variation among the different local populations that are different in biological characters.

Genetic Diversity and Thermostabilitical Variants of Corbicula japonica from the Two Main Rivers in Korea (한국의 두 강으로부터 재첩의 유전적 종다양성과 열안정성 변이체)

  • Heo, Man-Gyu;Mun, Du-Ho;Heo, Heung-Uk
    • Journal of Environmental Science International
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    • v.7 no.3
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    • pp.243-250
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    • 1998
  • We examined the genetic variation within the species, the patterns of genetic diversty between populations, thermostability variations of enzymes and temperature tolerances of Corbicula japonica from the two main rivers In Korea. Starch gel electrophoresis was used to examine the genetic variation of 22 locl. Henting experiments of electrophoresis under the condition of 40$\pm$5$^{\circ}$ for 15$\pm$5 min disclose thermostabllity differences, called heat-sensitive and heat-resistant types, within each 디ectrophoretic allozyme. Genetic diversity at the natural species level was high (77.3%), whereas the extent of heat-treat groups was relatively low (52.6%). The genetic diversity trends to decrease from the source of two main siderable high genetic diversity compared with a mean value of C. japonica species, It is recommended that several populations of the species in Korea should be preserved.

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