• The Journal of Internal Korean Medicine
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• v.25 no.3
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• pp.408-417
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• 2004

Objective : The aim is to identify the effects of Glycyrrhiza uralensis Fisch on immunocyte and cytokine production in asthmatic laboratory mice. This experiment was designed to investigate the antiallergic and antiinflamatary the effects of Glycyrrhiza uralensis Fisch on asthma. Materials and Methods : We measured the eosinophil, IL-4, IL-5, IL-13, $IFN-{\gamma}$, CD4, CDS, CD69, CCR3, CD11b, Gr-1 in bronchoalveolar lavage fluid of ovalbumin induced asthmatic mice. Results : Glycyrrhiza uralensis Fisch increased the proliferation of eosinophils, IL-4, IL-5, IL-13, IgE, granulocyte, CCR3, CD4, IgE, CD69. Glycyrrhiza uralensis Fisch increased the proliferation of $IFN-{\gamma}$. Conclusion : Results suggest that Glycyrrhiza uralensis Fisch extract is useful in treatment and prevention allergic asthma.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.89-89
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• 2019

Several studies report the anticancer effect of Glycyrrhiza glabra (G. glabra), Glycyrrhiza uralensis (G. uralensis) and their compounds. However, the anticancer effect of Glycyrrhiza cultivar roots are limited. In this study, we compared the anticancer effect of Glycyrrhiza cultivar (Wongam and Shinwongam) extracts with G. glabra and G. uralensis extracts in breast cancer cell lines. Freeze dried Glycyrrhiza root extracts were dissolved in cell culture media at 2 mg/mL and filtered by $0.2{\mu}m$ filter. Glycyrrhiza root extracts were serially diluted at the concentrations of $10{\mu}g/mL$, $100{\mu}g/mL$, $200{\mu}g/mL$, $400{\mu}g/mL$, $800{\mu}g/mL$, $1000{\mu}g/mL$ and $2000{\mu}g/mL$. MCF-7 and MDA-MB-231 breast cancer cells were treated with different concentrations of Glycyrrhiza root extracts and the cell viability was measured using MTT assay. In MCF-7 cells, G. glabra showed no significant difference with Wongam and showed significant difference with Shinwongam at $1000{\mu}g/mL$ (G. glabra 101.2% and Shinwongam 82.68%) and $2000{\mu}g/mL$ (G. glabra 83.07% and Shinwongam 54.05%). G. uralensis showed significant difference with Wongam at $2000{\mu}g/mL$ (G. uralensis 66.48% and Wongam 95.02%) and showed no significant difference with Shinwongam. In MDA-MB-231 cells, G. glabra showed no significant difference with both Wongam and Shinwongam. G. uralensis showed significant difference with Wongam at $2000{\mu}g/mL$ (G. uralensis 72.59% and Wongam 93.47%) and showed no significant difference with Shinwongam. In conclusion, the current study demonstrated that G, glabra and G. uralensis compared with Wongam, and Shinwongam at low concentrations ($10{\mu}g/mL{\sim}800{\mu}g/mL$) display similar cytotoxic potency.

• Journal of Haehwa Medicine
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• v.25 no.1
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• pp.145-153
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• 2016

Objectives : The purpose of this study is to evaluate the wound healing potential of Glycyrrhiza uralensis extract. Methods : Free radical scavenging activity tests for DPPH, peroxynitrite (ONOO) and hydroxyl radical (${\cdot}OH$) and total phenolic contents of Glycyrrhiza uralensis extract were conducted. Tube formation assay was performed using human umbilical vein endothelial cells (HUVECs). Results : The results showed that Glycyrrhiza uralensis extract exerted inhibitory effects on ONOO and ${\cdot}OH$. Tube formation in HUVEC was increase in a dose dependent manner. Conclusions : These results show the potential to promote the wound healing process by Glycyrrhiza uralensis extract.

• Korean Journal of Medicinal Crop Science
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• v.18 no.4
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• pp.231-237
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• 2010

The present study was conducted to investigate the anti-allergic reaction with Glycyrrhiza uralensis. We examined cell viability, $\beta$-hexosaminidase release, IL-4 and IL-13 mRNA expression from RBL-2H3 cell after pre-treatment with 0, 100, 250, 500, 1000${\mu}g/m{\ell}$ of Glycyrrhiza uralensis water extracts. Effects of Glycyrrhiza uralensis on the degranulation and pro-inflammatory cytokines (IL-4 and IL-13) expression were evaluated with $\beta$-hexosaminidase assay, and RT-PCR analysis. We observed that Glycyrrhiza uralensis concentrations from 100${\mu}g/m{\ell}$ to 1000${\mu}g/m{\ell}$ had no effect on cell survival. The release of $\beta$-hexosaminidase decreased significantly with all concentrations of Glycyrrhiza uralensis extracts. The expression of the IL-4 and IL-13 mRNA were decreased by Glycyrrhiza uralensis in dose-dependent manner. These results that Glycyrrhiza uralensis has an anti-histamin effects and controls IL-4, IL-13 secretion on allergic reaction.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.35-39
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• 2011

Objectives : Genetic analysis and taste pattern were performed to identify species between Glycyrrhiza uralensis and G. glabra which are officially listed in Korean Pharmacopoeia IX as origin of Gamcho(g$\={a}$nc$\v{a}$o, licorice root, Glycyrrhizae Radix et Rhizoma). Methods : Genetic analysis showed that identification between two species was done by comparing base sequence of ITS(intergenic transcribed spacer) and trnH-psbA regions from eleven Gamchoes sold in market. There was different taste pattern using by taste sensor in Glycyrrhiza uralensis and G. glabra. Results : Genetic analysis showed that six Gamchoes from China were identified as Glycyrrhiza uralensis and five Gamchoes from Uzbekistan were G. glabra. From the results of taste pattern, sourness and astringency of Glycyrrhiza uralensis from China were significantly higher than G. glabra from Uzbekistan, and aftertaste of astringency, aftertaste of umami, and saltiness of Glycyrrhiza uralensis were signicantly low as compared to G. glabra. There is no significant difference between two species in terms of bitterness, aftertaste of bitterness, and umami. Conclusions : Taken together, Glycyrrhiza uralensis from China and G. glabra from Uzbekistan were identified by taste sensor, and this technic could be applied to establishment of taste pattern marker for identification of different species located in various regions.

• Archives of Pharmacal Research
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• v.13 no.1
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• pp.103-104
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• 1990

An alkaloid was isolated from the roots of Glycyrrhiza uralensis Fisch. It was identified as 3-methyl-6, 7, 8-trihydro-pyrrolo[1, 2-alpyrimidin-2-one(I) by spectral analysis. The compounds is first found in the plant kingdom.

• Archives of Pharmacal Research
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• v.13 no.1
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• pp.101-102
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• 1990

Two alkaloids were isolated from the roots of Glycyrrhiza uralensis Fisch. They were identified as 5, 6, 7, 8-tetrahydro-2, 4-dimethylquinoline (1) and 5, 6, 7, 8-tetrahydro-4-methylquinoline (2) by spectral data. The isolation of alkaloid from glycyrrhiza species is reported for the first time.

• The Korea Journal of Herbology
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• v.36 no.3
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• pp.15-24
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• 2021

Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

• The Journal of Pediatrics of Korean Medicine
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• v.33 no.2
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• pp.12-21
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• 2019

Objectives Coptidis Rhizoma and Glycyrrhiza Uralensis are herbs that treat obesity and dampness-phlegm. The aim of this study is to investigate the efficacy of Coptidis Rhizoma and Glycyrrhiza Uralensis on lipid deposition with nonalcoholic fatty liver disease (NAFLD). Methods Male 6-week-old C57BL/6 male mice were divided into three groups: control group (Ctrl), high fat diet group (HFF), and high fat diet with Coptidis Rhizoma and Glycyrrhiza Uralensis extract administration group (CGT). Each 10 mice were allocated to each group (total of 30 mice). All mice were allowed to eat fat rich diet freely throughout the experiment. To examine the effect of Coptidis Rhizoma and Glycyrrhiza Uralensis, we observed weight changes, total cholesterol and glucose levels, lipid blot distributions, PGC-1, p-$I{\kappa}B$, and p-JNK. Results Body weights for all mice were measured and analyzed the difference between the groups. Weight gain was significantly lower in CGT group than the HFF group. Total cholesterol and glucose levels were significantly lower in CGT group. The distribution of lipid blots and positive reaction of PGC-1 were significantly lower in CGT group. The positive reaction of p-$I{\kappa}B$ in hepatic tissues was significantly lower in CGT group. The positive reaction of p-JNK in hepatic tissues was significantly lower in CGT group. Conclusions Coptidis Rhizoma and Glycyrrhiza Uralensis have the effect of improving non - alcoholic fatty liver induced insulin resistance through regulation of lipid metabolism.

• Korean Journal of Plant Taxonomy
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• v.51 no.4
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• pp.353-362
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• 2021

The chloroplast genome of Glycyrrhiza uralensis Fisch was sequenced to investigate intraspecific variations on the chloroplast genome. Its length is 127,689 bp long (34.3% GC ratio) with atypical structure of chloroplast genome, which is congruent to those of Glycyrrhiza genus. It includes 110 genes (76 protein-coding genes, four rRNAs, and 30 tRNAs). Intronic region of ndhA presented the highest nucleotide diversity based on the six G. uralenesis chloroplast genomes. A total of 150 single nucleotide polymorphisms and 10 insertion and deletion (INDEL) regions were identified from the six G. uralensis chloroplast genomes. Phylogenetic trees show that the six chloroplast genomes of G. uralensis formed the two clades, requiring additional studies to understand it.