• 대한간호학회지
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• 제9권2호
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• pp.17-26
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• 1979

This research is to prevent the infection of maternity in the hospital by examining the microbes contaminations in maternity through airbone microbes and those who are engaged in the ward of O.B. ＆ G.Y. and to furnish the basic data available to hospital management. The bacterial growth of airbone microbes contaminations in nosocomial air and who thor the nasal cavity of passers by (doctors, nurses, parturient women) who went to the ward of O.B. ＆ G.Y. contaminated or not were examined in“E”Univ. Hospital from July to August, 1979 by using thioglycollate broths and agar plates. The following results were obtained: 1. The average colony number of airborne microbes revealed as follows the pediatric ward (36 colonies), the internal ward (33 colonies), the ward of O.B. ＆ G.Y. (30 colonies), the ward of surgery (24 colonies), delivery-waiting room (11 colonies), and the delivery room (3 colonies). 2. The bacterial growth beforenoon differed from that of afternoon. Namely, the latter (24 colonies) was higher than the former (21 colonies). 3. The type of strains isolated from the air of the ward revealed staphylococci (82%), Gram negative bacilli (18%), fungi (17%), Gram positive diplococci (13%), and Bacillus subtilis (2.8%). 4. The strains isolated in the delivery-waiting room revealed staphylococci (66.7%), Gram negative bacilli (33.6%), and revealed staphylococci (75%), Gram positive diplococci (8.3%), and fungi (8.3%), in delivery room. 5. Most of strains isolated in the ward of O.B. & G.Y. revealed staphylococci (100.0%), Gram positive diplococci (8.3%), and Gram negative bacilli (6.7%). 6. The strain isolated in the surgical ward revealed staphylococci (91.7%), fungi (33.3%), Gram positive diplococci (25%), Gram negative bacilli (25%) and Bacillus subtilis (8.3%). 7. The strain isolated in the pediatric ward revealed staphylococci (75%), fungi (25%), Gram positive diplococci (8.3%), Bacillus subtilis (8.3%), and Gram negative bacilli (8.3%). 8. The strain isolated in the internal ward revealed staphylococci (91.7%), fungi (33.3%), Gram positive diplococci (25%), and negative bacilli (16.7%). The strains isolated from the nasal cavity of those doctors and nurses who and enaged in the ward of O.B. & G.Y. revealed staphylococci (80%), Bacillus subtilis (10%), and Gram negative bacilli (10%), from doctors and Gram positive diplococci (10%), instead of Gram negative bacilli (10％), from nurses. 10. The strain isolated from nasal cavity of parturient women on admission revealed staphylococci (90%), and Gram negative bacilli (10%), but after admission revealed staphylococci (70％), Gram positive diplococci (10%), and Gram negative bacilli (10%). 11. Of the total 91 staphylococci isolated from the air of the ward, the Coagulase pastive was 36 (39.6%), and the negative 55 (60.4%), As a result of the coagulase experiment of the staphylococci isolated from the nasal cavity of those who are engaged in the ward of O.B. ＆ G.Y. all were revealed as negative that belonged to non-pathogenic. 12. Consequence of the biochemic examination of the gram negative bacilli isolated from the air of the ward the aerobacter aerogens revealed was (16.7%) E-coli 5％ in the nasal cavity of those came and went to the of O.B. ＆ G.Y. and Aerobacter aerogens 7.5%.

• 대한임상검사과학회지
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• 제50권4호
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• pp.414-421
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• 2018

본 연구에서는 일개 대학병원에서 2년간 분리된 혈액배양 양성배지에서 MALDI-TOF MS system을 이용하여 Gram-positive bacilli를 동정한 결과를 균종별, 분기별로 분석하였다. Corynebacterium striatum은 총 89균주 중 66균주 (74.2%), Bacillus cereus는 60균주 중 44 균주 (73.3%), Listeria monocytogenes는 25균주 중 25균주 (100%)로 2.0이상의 높은 스코어에서 동정되었다. 미 동정 된 균주는 303균주 중 293균주는 혈액배양에서 1회 분리 균주로 감염균으로서의 의의가 없는 오염 균주로 간주되었다. 감염균으로서 의의가 있는 동일 환자 2회 이상 분리 균주 대상 16S-rRNA sequencing 비교결과 총 50균주 중 43균주가 일치해 86.0% 동정이 가능하였다. 일치하지 않은 7균주 중 5균주는 MALDI-TOF MS로도 동정이 되지 않았다. 결론적으로 혈액배양에서 Gram-positive bacilli가 동정되는 경우, 일차적으로 MALDI-TOF MS를 이용하여 동정해보고 이를 활용한다면 어렵고 비용이 많이 들던 Gram-positive bacilli 동정이 저비용으로 더욱 간편하고 정확해지며, Gram-positive bacilli에 의한 감염 진단에도 도움이 될 것으로 판단된다.

• 생명과학회지
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• 제9권3호
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• pp.314-327
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• 1999

This study was investigated to isolate identify the total bacteria and fungi from the indoor air of work-place of the shoes, paint, stainless steel, and plastic industries. The number of bacterial colonies on the nutrient agar plates were calculated by the open petridish method for 30 minutes in indoor air of work-places at the autumn and winter. The isolated bacteria were identified by Gram stain and biochemical test using API Staph and API 20E kits. The isolated fungal colonies were identified by gross appearance of the giant colonies and microscopic examination of their spore and hyphal characteristics on the slide culture method. The minimum inhibitory concentration (MIC) of several antibiotics against isolated bacteria was determined by the microdilution method with Mueller-Hinton broth. The 70-400 colonies in autumn and 54-236 colonies in winter were isolated from the indoor air of work-places of several industry. The isolation rates of Gram positive cocci, Gram positive bacilli, Gram negative bacilli, and Gram negative cocci were 46.3%, 19.8%, 17.3%, and 16.1%, respectively. In Gram positive cocci, the most strains were identified as Aerococcus spp, Micrococcus spp, and Staphylococcus spp. In Gram positive and negative bacilli, and Gram negative cocci were identified as Bacillus spp, Pseudomonas spp, and Neisseria spp, respectively. The frequently isolated fungi were Aspergillus spp, Penicillium spp and Rhizopus spp, respectively. The frequently isolated Aerococcus spp, Micrococcus spp, and Staphylococus spp were highly resistance against ampicillin, erythromycin, methicillin, and tetracycline. These results arouse our attention to microbiologic pollution in the indoor air of work-places of industries.

• Journal of Yeungnam Medical Science
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• 제2권1호
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• pp.183-190
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• 1985

본 시안(MCRCODE-N)은 말 그대로 시안인 바 많은 수정과 첨가가 필요할 것이라고 생각되나 국내 실정에 맞는 검사법이라고 생각된다. 본 시안을 이용하여 결정을 보완하는 일이 시급한 바 동학 선후배의 지도 편달을 바라는 바 이다.

• 미생물학회지
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• 제46권2호
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• pp.152-156
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• 2010

연성 콘택트렌즈 종류의 하나인 일회용 렌즈는 사용상 편리함을 주는 반면 다양한 안과적 감염성 질환을 초래한다. 안 질환을 유발하는 미생물로는 Acanthamoeba, Bacteria, Fungi 등이 있다. 렌즈 착용시 손을 이용함으로 다양한 미생물 오염은 근절이 불가피한 실정이다. 다양한 미생물의 오염으로 인해 감염성 각막염을 유발하나 정확한 미생물 동정은 미비한 상태이다. 이런 이유로 렌즈를 세척하기 위해 흔히 사용하는 식염수와 ReNu Solution (Bausch and Lomb Company, USA)의 2그룹으로 나누고 식염수와 ReNu Solution을 사용하여 1, 3, 5 10 그리고 15일에 따라 미생물 오염의 정도를 살펴보고 분리되는 미생물을 동정하고자 하였다. 대구시 D대학 안경광학과 재학생들의 안과적 건강상태를 조사한 후 그룹별 10명씩 지정하였다. 일일 착용렌즈를 1일, 3일, 5일 10일, 그리고 15일에 따라 사용 후 배지에서 세균을 배양하였다. 배양된 집락을 선택하여 그람 염색한 결과, Gram-positive cocci 33%, Gram-negative cocci 2%, Gram-positive bacilli 34%, 그리고 Gram-negative bacilli 31%로 나타냈고 이러한 세균을 동정하기 위해 API kit와 VITEK system을 이용하였다. 그 결과 콘택트렌즈의 착용에 의해 유발되는 각막염의 원인세균으로 알려진 S. aureus, P. aeruginosa 등이 분리되었다. 본 연구에서는 연성렌즈의 하나인 일일 착용렌즈의 착용시 세척액에 따른 일반세균의 분포와 일회용 렌즈의 연속 착용에 따른 병원성 세균을 조사함으로 콘택트렌즈의 사용시 위생의 필요성을 시사한다.

• 한국환경보건학회지
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• 제19권1호
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• pp.30-36
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• 1993

A survey was conducted to measure concentration of airborne microbe in a hospital using RSC air sampler during October~November 1991.The result was as follows: 1) In an agar strip GK-A media for total counts of microbial particles. The highest count were 1384 CFU/m$^3$ in the main lobby, followed by 912 CFU/m$^3$, in the obstetric room, 688 CFU/m$^3$ in 1CU. By gram staining, the distribution for organisms in the air were shown 74.1% in gram possitive cocci followed by 16.8%, in gram possitive bacilli 6.7% in gram negative bacilli and 4.7% in yeast, but low organism was detected in recovery room with 194 CFU/m$^3$. 2) In agar strip S media for Staphylococci the count at the main lobby was detected in the recovery room with 92 CFU/m$^3$, Tests of coagulase were negative Staphylococci with 78%, and positive Staphylococci with 22%. The Staphylococci were highly resistance to penicillin, ampicillin and sensitive to amikacin, cefazolin, gentamycin and chloramphenicol. 3) In agar strip C media for coliform bacteria the colony counts at the main lobby was 139 CFU/m$^3$ and treatment room was 190 CFU/m$^3$, most frequently isolated microorganisms were non fermentative bacilli. 4) In agar strip HS media for yeast and molds. Most frequently colony counts 17~76 CFU/m$^3$, 0.5% lactophenol cotton blue stains were shown unidentified 77.2%, 8.1%, in Penicillium 8.1% in Aspergillus, and 3.8% in mucor.

• 대한임상검사과학회지
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• 제56권1호
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• pp.59-65
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• 2024

제주특별자치도에 서식하는 등줄쥐를 포획하여 대장을 포함한 내부장기에서 분변 검체를 채취한 후, 장내 미생물을 호기적 배양하여 등줄쥐 분변에서 배양된 장내 미생물 분포를 조사하였다. 등줄쥐 분변 36개의 검체를 호기성 배양된 세균 집락을 그람염색 한 결과 모든 검체에서 그람음성막대균이 우세하게 관찰되었다. 36 검체 중 그람음성막대균은 36 균주(100%), 그람양성알균은 21 균주(58.3%), 그람양성막대균은 15 균주(41.7%)로 나타났으며 그람음성알균은 관찰되지 않았다. 36개의 검체에서 배양된 그람음성막대균은 Vitek 2 system을 이용하여 동정한 결과 모두 Escherichia coli (E. coli) 균종으로 동정되었으며, 1개 검체는 E. coli 균종과 Enterobacter cloacae 균종이 동시에 동정되었다. 분리된 E. coli 36 균주 중 항균제에 내성을 보이는 균주는 발견되지 않았으며, 1 균주만 cefoxitin 항균제에 중등도 내성을 보이는 것으로 나타났다. 진드기, 설치류 매개 감염병의 원인이 되는 등줄쥐의 분변에서는 병원성 세균은 존재하지 않았다.

• 대한의생명과학회지
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• 제24권2호
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• pp.108-115
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• 2018

In the present study, results of the identification of Gram-positive bacilli (GPB) were analyzed by using the MALDI-TOF MS technique to score each 2-year blood culture at a university hospital. In addition, 16S rRNA sequence analyses and MALDI-TOF MS results are compared to targeting strains that had been isolated two or more times within the same patient, to evaluate the usefulness of MALDI-TOF MS in GPB identification. According to the cut-off (${\geq}1.7$) criteria, there were 410 (57.5%) reliable strains and 303 (42.5%) non-identified strains among the GPB identification results of 713 strains, using a microflex MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany). The isolation appeared most often in the following order: Corynebacterium striatum, Bacillus cereus, Bacillus subtilis, Paenibacillus urinalis, and Listeria monocytogenes. Nearly three-fourths, 66 out of 89 (74.2%) of the strains for Corynebacterium striatum; 44 out of 60 (73.3%) strains for Bacillus cereus; and all (25 out of 25, 100%) Listeria monocytogenes strains were identified by their high scores of 2.0 or higher. Most (293 strains out of 303) non-identified strains were strains isolated only once and not significant as infectious bacilli. A total of 43 out of 50 (86.0%) strains matched and were able to be identified based on the 16 rRNA sequencing comparison results of strains that were isolated twice or more within the same patient and significant as infection bacilli. Non-matching among 5 out of 7 strains was not identified, even with MALDI-TOF MS. In conclusion, GPB can be identified in blood cultures using MALDI-TOF MS. This can be done accurately with ease, rapidly, and at a low cost. It is also thought to be helpful in GPB diagnosis and treatment.

• Journal of Species Research
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• 제8권2호
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• pp.176-190
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• 2019

A total of 38 bacterial strains within the classes Bacilli and Deinococci were isolated from various sources in Korea. Samples were collected from animal intestine, urine, soil, tidal flat mud, and kimchi. In the sequence comparison and phylogenetic analysis of 16S rRNA sequences, the 38 isolates were assigned to the classes Bacilli and Deinococci with sequence similarities more than 98.7%. Twenty-four strains and 13 strains were classified the order Bacillales and Lactobacillales in the class Bacilli, respectively. In the order Bacillales, there were nine species in the genus Bacillus, seven species in the genus Paenibacillus, and the remaining eight species in the genera Domibacillus, Halobacillus, Virgibacillus, Lysinibacillus, Paenisporosarcina, Planococcus, Savagea, and Staphylococcus. In the order Lactobacillales, there were four species in the genus Lactobacillus, three species in the genus Leuconostoc, three species in the genus Lactococcus, and the remaining three species in the genera Aerococcus, Enterococcus, and Streptococcus. One species was related to the genus Deinococcus of the order Deinococcales. Most of the isolated strains were Gram-stain-positive, but some were Gram-stain-variable or Gram-stain-negative. Cells were rod or cocci-shaped. Based on the results of 16S rRNA analysis, we report 38 strains as previously unrecorded species to Korea, and the basic characteristics of strains are described herein.

• 대한간호학회지
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• 제14권2호
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• pp.55-62
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• 1984

This study was performed from september 26th to October 7th 1981 to investigate the contamination problems of Nurse's hands characterized by var-ious nursing functions. A total of 50 nurse's hands were sampled from 5 different wards of H. University Hospital. The samples were cultured for isolation of microorganisms. The results were as follows: 1. Of 50 Nurses 23 were found to be contaminated by 9 species of bacilli such as Non-fermentative gram negative Bacilli, Gram negative bacilli, Oxidase positive, Enterobacter, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter, Staphylococcus epidermidis, Gaffkya tetragens, Bacillus subtilis. 2. The contaminate rates by wards where they have been serving are; 7 (87.5％) of 8 nurses from Intensive care unit, 7(70%) of 10 nurses from general surgery ward, 3(50%) of 6 nurses from neurosurgery ward, 2(20%) of 10 nurses from orthopedic surgery ward, 4(25%) of 10 nurses from medical ward. 3. The contamination rates by the types of clinical service offered are 6(85.7%) of 7 nurses after wound dressing assist 6 (55.3%) of 13 nurses after vital sign check. 4. No statistical significance could he observed as to the between the rates of contamination of nurse's hands with various nursing functions (0.1