• Title/Summary/Keyword: HI titer

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The comparative study on Newcastle disease virus antibody titer by hemagglutination inhibition test and enzyme-linked immunosorbent assay (혈구응집억제반응과 효소면역측정법을 이용한 닭 뉴캣슬병 바이러스에 대한 혈중항체가 비교)

  • Han, Sung-Tae;Lee, Cheong-San;Kwak, Hak-Koo;Song, Jong-Han;Lee, Jong-In
    • Korean Journal of Veterinary Service
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    • v.26 no.3
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    • pp.215-219
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    • 2003
  • This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA) titers and sample to positive ratio (S/P ratio) of Newcastle disease(ND) virus. To perform this study, the 372 sera of broiler chicks and 120 sera of layers and breed chicks were collected from slaughter house and farms, respectively. As a result of HI test out of different chicks, the positive percentage of ND antibody titer of broiler, layer and breeder, when a standard positive HI titer were '2', was 84.4%, 100% and 100%, respectively. The positive percentage of ND antibody titer by ELISA was shown 38.4%, 100% and 100% and S/P ratio were also shown 81.5%, 98.2% and 99.2%, respectively. The results of comparative survey with same sera by two experimental methods were as follows; In low HI titer, ELISA titer was not similar to HI titer, but S/P ratio was similar to it. In high HI titer, ELISA titer was not similar to HI titer. Therefore, HI titer was more similar to S/P ratio than ELISA titer.

Influence of Hermetia illucens Larvae-Derived Functional Feed Additives on Immune Function of Broilers (동애등에(Hermetia illucens)유충 유래 기능성 사료첨가제가 육계 면역기능에 미치는 영향)

  • Ji, Sang-Yun;Park, Kwan-Ho;Kim, Ki-Hyun;Lee, Heui-Sam;Choi, Gyu-Sung;Lim, Yeon-Ji;Yu, Ri;Choi, In-Hag;Chung, Tae-Ho
    • Journal of Environmental Science International
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    • v.27 no.12
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    • pp.1305-1307
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    • 2018
  • We investigate the effects of the immune function (HI titer) in broilers fed diets containing Hermetia illucens (H. illucens) peptide extract over a 40-day period. Twenty-four broiler chicks (Arbor Acres, 1 d old) were divided into four groups and fed different diets (control, 0.1%, 0.5%, and 1% H. illucens peptide extract). To evaluate HI titer, all broilers were vaccinated with H9H2 vaccine subcutaneously on the lateral thorax, according to the manufacturer's recommendations. Similar HI titer was observed with 1% H. illucens peptide extract treatment compared to the control after 40 days (p>0.01). Groups fed 0.5% H. illucens peptide extract demonstrated the most effective immune effects (p<0.01), followed by groups fed 0.1% H. illucens peptide extract. In conclusion, using 0.1% or 0.5% H. illucens peptide extract before or after vaccination improved HI titer immune function in broilers.

A survey of newcastle disease virus antibody titers on slaughtered broiler chicks (도축 육계에서 뉴캣슬형 바이러스에 대한 혈중항체가 조사)

  • 이정원;허철호;이종환;권정택;송희종
    • Korean Journal of Veterinary Service
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    • v.24 no.3
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    • pp.217-222
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    • 2001
  • Hemagglutination inhibition(HI) titers of Newcastle disease(ND) were measured to investigate the vaccination times on three different species of broiler chicks in Chonbuk province. Each 330 of Cobb, Ross and White-semi broiler chicks were selected from 11 broiler farms. The primary vaccine were sprayed in hatchery at one day old chicks. Secondary and tertiary vaccine were used by drinking water at 7 to 24 days old chicks. The ND antibody titer were measured by HI from each different species of broiler chicks at the marketing date. Total average HI titers of Cobbs vaccinated with primary ones, secondary and tertiary ones were recorded 1.86, 1.52 and 2.76, respectively. The antibody titers were shown to 2.22, 2.13, 3.07 in terms of vaccination of Ross broiler chicks. They were also 2.56, 2.65 and 2.78 in terms of vaccination of White-semi broiler chicks. The value HI titer were not statistically different of all treatments. The results of this experiment suggested that HI titer of sera is scored less than defensive value of ND antibody titer at more than two times of vaccination.

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Serological study on canine parvovirus in the puppies (신생자견에 있어서 Canine parvovirus에 대한 혈청학적 연구)

  • 박경옥;김상윤;조옥숙;김정화;김대원
    • Korean Journal of Veterinary Service
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    • v.21 no.1
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    • pp.87-95
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    • 1998
  • The present study was conducted to characterize maternal antibody status which haemagglutination inhibition(HI) titers against canine parvovirus(CPV) in the 15 puppies delivered from 3 dams. The range of HI titers of 5 puppies delivered from a mother dog(A) with HI titer of 1 : 1,024 were 1 : 16~1 : 64 at 1 day old before suckling, 1 : 512~1 : 1,024 at 2 days old after suckling, 1 : 512~1 : 2,048 at 1 week old, 1 : 256~l : 1,024 at 2 weeks old, 1 : 128~l : 512 at 3 weeks old, 1 : 128~l : 256 at 4 weeks old, 1 : 32~1 : 128 at 5 weeks old, 1 : 16~1 : 64 at 6 weeks old, 1 : 16~1 : 64 at 7 weeks old, and 1 : 16~l : 32 at 8 weeks old. After vaccination with DHPPL to canine parvovirus in 60 days and 80 days old puppies, 1 : 8~l : 32 at 9 weeks old, 1 :16~1 : 128 at 10 weeks old, 1 : 32~1 : 256 at 11 weeks old, 1 : 16~1 : 256 at 12 weeks old, 1 : 128~1 : 256 at 13 weeks old, 1 : 64~l : 512 at 14 weeks old, and 1 : 128~1 : 512 at 15 weeks old. The HI titers of 3 puppies delivered from a mother dog(B) with HI titer of 1 : 512 were 1 : 16 at 1 day old before suckling, 1 : 256~1 : 512 at 2 days old after suckling, 1 : 512 at 1 week old, 1 : 128~1 : 256 at 2 weeks old, 1 : 64~1 : 128 at 3 weeks old, 1 : 64~1 : 128 at 4 weeks old, 1 : 128 at 5 weeks old, 1 : 64~1 : 128 at 6 weeks old, 1 : 16 at 7 weeks old, and 1 : 8 at 8 weeks old. After vaccination with DHPPL to canine parvovirus in 60 day and 80 days old puppies, < : 8~l : 8 at 9 weeks old, < : 8 ~1 : 16 at 10 weeksold, 1 : 64~1 : 128 at 11 weeks old, and 1 : 256~1 : 512 at 12 weeks old. The HI titers of 7 puppies delivered from mother dog(C) with Hl titer 1 : 1,024 were 1 : 512~1 : 1,024 at 2 days old after suckling, 1 : 256~1 : 1,024 at 1 week old, 1 : 256~l : 1,024 at 2 weeks old, 1 : 64~1 : 512 at 3 weeks old, 1 : 64~1 : 512 at 4weeks old, 1 : 8~l : 64 at 5 weeks old, 1 : 8~1 : 64 at 6weeks old, 1 : 8~1 : 32 at 7 weeks old, and < : 8~1 : 8 at 8 weeks old. Antibody to CPV was transferred mainly from mother to progeny through the colostrum and the transferred maternal antibody was in proportion to the HI titer of the mother As the HI titer of maternal antibody in puppies was low, puppies have a rapid immune response and a massive rise in HI titer to vaccination against CPV compared with puppies haying high level of maternal antibody.

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Comparison of pooled Versus Individual Sera in Avian Infectious Bronchitis Virus Seroprevalence Study (닭 전염성 기관지염 바이러스의 혈청 유병률 연구에서 개별혈청과 합병혈청의 비교)

  • Kim, Sa-Rim;Kwon, Hyuk-Moo;Sung, Haan-Woo;Pak, Son-Il
    • Journal of Veterinary Clinics
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    • v.23 no.4
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    • pp.416-420
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    • 2006
  • Compare to testing sera individually, pooled-serum testing has considered as a cost-effective method, particularly on a large population-based seroprevalence studies. This study was to determine the relationship between individual sera and pooled sera titers for detection of avian infectious bronchitis virus (IBV) and to evaluate suitability of pooled sera by comparing prevalences estimated from both samples. A total of 5,000 individual samples were collected from 500 flocks in Chungcheong, Gyunsgi, and Kangwon provinces between January 2005 and February 2006. Ten samples were randomly selected from each flock. Five-hundred pooled sera were prepared by mixing equal amount of each 10 individual serum from the original samples. IBV antibody titers were measured by hemagglutination inhibition (HI) test. The least squares regression analysis was performed to construct equation between pooled and mean individual titers. To determine whether the flock is infected 4 arbitrary criteria were used: detection of at least 1 chicken with HI titer ${\ge}$ 9 (criterion 1), detection of at least 2 samples with HI titer ${\ge}$9 (criterion 2), detection of at least 1 sample with HI titer ${\ge}$ 10 (criterion 3), and filially detection of at least 1 sample with HI titer ${\ge}$ 11 (criterion 4). The receiver operating characteristic (ROC) curve was used to examine the cut-off points of pooled titers showing optimal diagnostic accuracy. The area under the curve (AUC), sensitivities (Se), specificities (Sp), and positive (PPV) and negative (NPV) predictive values were calculated. The regression equation between pooled titers (pool) and mean individual titers (mean) was: $pool= 1.2498+0.8952{\times}mean$, with coefficient of determination of 87% (p< 0.0001). The optimal cut-off points of pooled titers were titer 8 for criterion 1 (AUC=0.975, Se=0.883, Sp=0.959, PPV=0.985, NPV=0.728), titer 8 for criterion 2 (AUC=0.969, Se=0.954, Sp=0.855, PPV=0.926, NPV=0.907), titer 9 for criterion 3 (AUC=0.970, Se=0.836, Sp=0.967, PPV=0.978, NPV=0.772), and titer 9 for criterion 4 (AUC= 0.946, Se=0.928, Sp=0.843, PPV=0.857, NPV=0.921). The difference of 'prevalence estimated by individual and pooled sample showed a minimum of 2% for criteria 2 and a maximum of 9.1:% for criteria 3. These results indicate that the use of pooled sera in HI test for screening IBV infection in laying hen flocks is considered as a cost-effective method of testing large numbers of samples with high diagnostic accuracy.

Epidemiological Observation on Recent Outbreaks of Canine Parvoviral Enteritis in Korea (최근 국내발병 개 파보바이러스성 장염에 대한 역학적 조사)

  • Jeoung, Seok-Young;Kim, Doo;Ahn, So-Jeo;Pak, Son-Il
    • Journal of Veterinary Clinics
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    • v.23 no.3
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    • pp.223-229
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    • 2006
  • Recently canine parvovirus(CPV) enteritis had been controlled successfully by the use of low-passage high titer modified live CPV vaccines. However, outbreaks of CPV enteritis have been continued in Korea. In this study, we carried out epidemiological investigation on the recent outbreaks of CPV enteritis of dogs and determined the potential prognostic factors affecting the survival of dogs. The total of 140 dogs diagnosed for CPV enteritis were statistically analysed. The majority of dogs were from 6 to 18 weeks of age and were not vaccinated or incompletely vaccinated. There were significant difference in the survival rate between male and female dogs with CPV enteritis and among the age groups(p<0.05). Moreover, there was significant difference in the survival rate of dogs between HI titer <80 group and HI titer 160 group(p<0.05). The majority of dogs had a history of diarrhea, vomiting, lethargy and dehydration. It was considered that recent outbreak of CPV enteritis in Korea caused by the failure of vaccination and/or by the inadequate antibody responses to CPV vaccines. Prophylactic measures should include isolation of young dogs from the dog population until the vaccination can be expected to provide protection.

Serum Parvovirus Antibody Titers among Healthy Adult Dogs in Korea (국내 성견의 개 파보바이러스에 대한 항체가 조사)

  • Jeoung, Seok-Young;Kim, Doo;Ahn, So-Jeo;Pak, Son-Il
    • Journal of Veterinary Clinics
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    • v.23 no.3
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    • pp.308-313
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    • 2006
  • This study was performed to monitor the level of serum canine parvovirus(CPV) antibody titers in adult dogs throughout the Korea from January 2003 to April 2004. A total of clinically healthy 885 dogs between 1 year and 17 years old were included in this study. Serum antibody titers against CPV were measured by means of hemagglutination inhibition(HI) titers at the time dogs were brought to the hospital for revaccination. Most of dogs had been primarily vaccinated or previously revaccinated. Dogs were grouped by age, breed, sex, and primary vaccination and revaccination to determine whether these factors were associated with antibody titers. Serum CPV titers ${\geq}80$ were considered protective. Protective antibodies against CPV were present in 95.0% of the population. Breed, age, and primary vaccination and revaccination were not significantly associated with serum CPV antibody titers. But sex was significantly associated with CPV antibody titers. The results of this study have shown that there is a need to reconsider the annual revaccination strategy against CPV infection.

Epizootiologic Surveys on Canine Parvovirus Infections of Dogs Kyungbuk West Area (경북서부지역에서의 개 파보바이러스 감염상황 조사)

  • 김성국;이정아;오강희;박영구
    • Korean Journal of Veterinary Service
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    • v.17 no.3
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    • pp.174-180
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    • 1994
  • The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Kyungbuk West Area since March 1990 and it was followed serologically for the classification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in Hemagglutinating inhibition test. The result obtained were summarized as follows : 1. During 5 years(March. 1990∼September. 1994), 1,470 dogs were investigated on the actual condition of CPV infections. The Infection rate of CPV from dogs was 62.5% and mortality rate was 59.8%. 2. Among 24 fecal samples collected from the dogs with enteric disease, all showed the hemagglutinating activity to porcine erythrocyte ranging from 40 to 5,120 of HA titers. 3. Among 12 sera samples collected from the dogs with enteric disease, all showed the serological identity with the reference CPV antigen from 5 to 5,120 of HI titers. 4. Bacteriologic examination of fecal specimens resulted in the isolation of pathogeric bacteria such as Staphylococcus sp, Streptococcus sp, Escherichia coli and Bacillus. Cultures for salmonella sp and Clostridium remaind negative. 5. The prevalence and identification of internal parasites were determined by fecal examination using the floatation methods. From 20 fecal samples 12(60.0%) were isolated and their species were Toxacara canis, Toxascaris leonina, and coccidium.

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Comparative study on avian influenza virus antibody titer by hemagglutination inhibition test and enzyme-linked immunosorbent assay in the mass zone layer (산란계 밀집지역에서 혈구응집억제반응과 효소면역측정법을 이용한 가금인플루엔자 혈중항체가의 비교 조사)

  • 이정원;엄성심;이성재;서이원;서석열;정동석;송희종
    • Korean Journal of Veterinary Service
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    • v.27 no.1
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    • pp.81-87
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    • 2004
  • This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja;Joh, Seong-Joon;Choi, Kang-Seuk;Kim, Aeran;Seo, Min-Goo;Song, Jae-Young;Yun, Seon-Jong
    • Korean Journal of Veterinary Research
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    • v.56 no.3
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    • pp.189-192
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    • 2016
  • The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.