• Journal of Microbiology
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• v.43 no.spc1
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• pp.110-117
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• 2005

Salmonella enterica serovar Typhimurium causes human gastroenteritis and a systemic typhoid-like infection in mice. A critical virulence determinant of Salmonella is the ability to invade mammalian cells. The expression of genes required for invasion is tightly regulated by environmental conditions and a variety of regulatory genes. The hilA regulator encodes an OmpR/ToxR family transcriptional regulator that activates the expression of invasion genes in response to both environmental and genetic regulatory factors. Work from several laboratories has highlighted that regulation of hilA expression is a key point for controlling expression of the invasive phenotype. A number of positive regulators of hilA expression have been identified including csrAB, sirA/barA, pstS, hilC/sirC/sprA, fis, and hilD. HilD, an AraC/XylS type transcriptional regulator, is of particular importance as a mutation in hilD results in a 14-fold decrease in chromosomal hilA::Tn5lacZY-080 expression and a 53-fold decrease in invasion of HEp-2 cells. It is believed that HilD directly regulates hilA expression as it has been shown to bind to hilA promoter sequences. In addition, our research group, and others, have identified genes (hilE, hha, pag, and lon) that negatively affect hilA transcription. HilE appears to be an important Salmonella-specific regulator that plays a critical role in inactivating hilA expression. Recent work in our lab has been directed at understanding how environmental signals that affect hilA expression may be processed through a hilE pathway to modulate expression of hilA and the invasive phenotype. The current understanding of this complex regulatory system is reviewed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.7
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• pp.949-955
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• 2018

Hardware-in-the-loop (HIL) simulation is a technique that can be employed for developing and testing complex real-time embedded systems. HIL simulation provides an effective platform for verifying power management system (PMS) performance of liquefied natural gas carriers, which are high value-added vessels such as offshore plants. However, HIL tests conducted by research institutes, including domestic shipyards, can be protracted. To address the said issue, this study proposes a field programmable gate array (FPGA) based PMS-HIL simulator that comprises a power supply, consumer, control console, and main switchboard. The proposed HIL simulation platform incorporated actual equipment data while conducting load sharing PMS tests. The proposed system was verified through symmetric, asymmetric, and fixed load sharing tests. The proposed system can thus potentially replace the standard factory acceptance tests. Furthermore, the proposed simulator can be helpful in developing additional systems for vessel automation and autonomous operation, including the development of energy management systems.

• The Journal of Korean Institute for Practical Engineering Education
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• v.3 no.2
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• pp.100-105
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• 2011

By adopting HIL(Hardware-in-the-Loop), component characteristics in vehicle environment can be obtained without implementing component in the vehicle. In this paper, when specific motor is adopted as traction motor in hybrid electric vehicle HIL implementation procedures are explained. In order to implement HIL method motor testing. vehicle performance simulator and load characteristic are explained. Vehicle controller used in simulator is directly uploaded in real controller. Especially as a load dynamometer actively controlled motor system is used without connecting conventional mechanical inertia. Motor characteristics are obtained using HIL implementation when test motor is used as a traction motor for parallel hybrid electric vehicle. Proposed method can be used as experimental equipment to educate driving characteristics of hybrid electric vehicle.

• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.70-75
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• 2012

In Salmonella enterica, many genes encoded within Salmonella pathogenicity islands (SPI) 1 and 2 are required to cause a range of diseases in a variety of hosts. The SPI1-encoded regulator HilD activates both the SPI1 and 2 genes at different times during growth in Luria-Bertani (LB) media. In this study, the expression levels of hilD during growth in LB were investigated. The data suggest that hilD expression is induced in the early stationary phase and decreases in the late stationary phase, when sseA, an SPI2 gene, is maximally expressed. However, HilD could act as an activator of sseA expression in the late stationary phase despite being present at low levels. SseA expression was investigated in SPI1 regulator mutant strains, hilA, hilD and invF mutants. As expected, hilD mutation decreased sseA expression. However, we found that invF mutation caused a 1.5-fold increase in sseA expression in not only LB but also M9 minimal media, which is thought to resemble an intracellular environment. InvF overexpression restored sseA expression to wild-type levels in an invF mutant but did not cause an additional reduction in sseA expression. These results suggest that SPI1 controls SPI2 expression either positively or negatively.

• Proceedings of the KIPE Conference
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• 2013.11a
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• pp.37-38
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• 2013

This paper presents an intuitive and powerful way to study and design motor drive control. The control of induction motors, as most widely used machines, is discussed. Thanks to ultra low latency and high fidelity Hardware-in-the-Loop systems, different aspects of up-to-date drive regulation are examined. A power stage, comprised of a grid voltage source, a rectifier, a VSC inverter and an induction motor, is emulated on the HIL platform in real time. A digital signal controller is plugged into the interface board and connected to the HIL emulation platform, without any hardware modifications. For motor control and power electronics applications, a dedicated Texas Instruments TMS320F2808 DSP is chosen. The same controller can drive an emulation platform and a real device with no modifications. Current and speed control loop test results are presented and discussed.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1063-1076
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• 2022

Two experiments were conducted to determine the effect of Hermetia illucens larvae (HIL) as protein and protease on growth performance, blood profiles, fecal microflora, and gas emission in growing pig. In experiment 1, the seventy-two crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial body weight (BW) of 27.98 ± 2.95 kg were randomly allotted to one of four dietary treatments (3 pigs per pen and 6 replicates pen per treatments). The experimental design was a 2 × 2 factorial arrangement of treatments evaluating two diets (Poultry offal diets and HIL diets) without or with supplementing protease. The poultry offal in basal diet has been replaced by HIL. In experiment 2, the four crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial BW of 28.2 ± 0.1 kg were individually accepted in stainless steel metabolism cages. The dietary treatments included: 1) PO- (PO-; poultry offal diet), 2) PO+ (PO- + 0.05% protease), 3) HIL- (3% PO of PO- diet was replacement 3% HIL), 4) HIL+ (HIL- + 0.05% protease). In experiment 1, From weeks 0 to 2, average daily gain (ADG) and feed efficiency (G:F) were significantly increased in the PO diet group compared with the HIL group. From weeks 2 to 4, ADG and G:F were higher for protease group than for non-protease group. At weeks 2 and 4, the PO diet group had lower blood urea nitrogen (BUN) levels than HIL diet group. In experiment 2, crude protein (CP) and nitrogen (N) retention were decreased by HIL diet at weeks 2 and 4. The fecal microflora and gas emission were not affected by HIL and protease. The HIL diet showed lower CP digestibility than PO diet and total essential amino acids digestibility tended to higher in PO diet than HIL diet. In summary, the present study revealed that replacement of the PO protein with the HIL protein and the additive of protease in growing pig diets during the overall experimental period had no negative effect.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.4745-4750
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• 2011

A HIL(hardware-in-the-loop) system was established and the simulation was carried out to determine whether the system operates normally. The system consists of turbocharger test bench, HIL platform with real time S/W and DAQ, and engine model using Matlab/Simulink. In the simulation the supplied fuel rate is changed step-by-step from 1.8944 kg/h to 4.7360 kg/h. The change of air-fuel ratio is analyzed and observed whether the air-fuel ratio follow the target air-fuel ratio 32. When the supplied fuel rate is changed, the air-fuel ratio is converged to the target air-fuel ratio after about 20 seconds. And the vane duty ratio of turbine and the boost pressure of compressor are also changed properly. Therefore this HIL system can be used to develop the new turbocharger and improve the performance of the modified turbocharger.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2006.11a
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• pp.319-322
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• 2006

The Deep-sea miner system is composed of body, actuators, sensors, and devices for control and monitoring. At present, we are manufacturing the miner's body included actuators and already consisted with off-the-shelf embedded controller. But sensors and those devices were just determined. To previously test performance of embedded controller which manages control and monitoring of miner system, its simulator must be developed for control and monitoring. Hardware-In-the-Loop(HIL) simulation is being increasingly used in industrial applications. This is an effective tool for the evaluation of electric system and drives. In the HIL simulator, we can test and design the control and monitoring system freely without the risk of hardware ruins and the load of expenses. Also the programming software for miner operating is verified on the HIL simulator. In this paper, we introduce the concept of HIL simulator for control and monitoring of deep-sea miner.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1589-1590
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• 2007

본 논문에서는 대체에너지 발전시스템이 포함되는 복합발전시스템의 구성 및 운전 특성 등을 분석하고 종합적인 엔지니어링 기술 확보에 필요한 Hardware-in-the-loop(HIL) Simulator의 구조를 제시하며 이를 효율적으로 제어할 수 있는 운영 소프트웨어를 개발하였다. 개발된 HIL Simulator는 복합발전 시스템을 구성하는 서브시스템의 제어기를 설계하는데 유용하게 사용할 수 있다.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07b
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• pp.1619-1622
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• 2005

The ultra thin hole injection layer (HIL) was deposited on an indium-tin-oxide (ITO) anode by using an ion beam assisted d eposition (IBAD) for the fabrication of an polymeric electroluminescence device for the first time. The device with the HIL deposited by IBAD has higher external quantum efficiency than the device with the HIL by conventional thermal evaporation. It is found that the deposited HIL by IBAD has high surface coverage on ITO anode in a few nm regions because the HIL prepared has high adatom mobility by ion beam energy.