• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.105-113
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• 2011

The present study investigated the nuclear remodeling, development potential with telomerase activity and transcription level of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos using two different fusion and activation methods. Female adult fibroblasts were injected into perivitelline space of in vitro matured oocytes. The oocyte-nucleus complexes were fused and followed by immediately either activated (Group 1), or activated at 1 h post-fusion (hpf) (Group 2), respectively. The incidence of normal premature chromosome condensation (PCC) at 1 hpf was slightly increased in the Group 2, compared to those of Group 1, but there was no significant (p<0.05) difference. The incidence of normal pronucleus (PN) and chromosome spread at 5 and 18 hpf were significantly (p<0.05) higher in the Group 2 than those of Group 1. The cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cell numbers were significantly (p<0.05) higher in the Group 2, compared to those of Group 1. Level of telomerase activity was significantly (p<0.05) higher in the SCNT blastocysts of Group 2, compared to those of Group 1. Transcript levels of HPRT, MeCP2 and XIST were not significantly (p<0.05) different between blastocysts of Group 1 and 2. However, transcript level of ANT3, RPS4X, XIAP and ZFX were significantly (p<0.05) up-regulated in the SCNT blastocysts of Group 2, compared to those of Group 1. Taken together, it is concluded that oocyte activation at 1 hpf induces the enhanced developmental potential by efficient nuclear remodeling and subsequent facilitation of the nuclear reprogramming of bovine SCNT embryos.

• The Transactions of the Korea Information Processing Society
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• v.6 no.4
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• pp.901-915
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• 1999

Parallel computers are known to be excellent in performance per cost also satisfying scalability and high performance. However parallel machines have enjoyed limited success because of difficulty in parallel programming and non-portability between parallel machines. Recently, researchers have sought to develop data parallel language that provides machine independent programming systems. Data parallel language such as High Performance Fortran provides a basis to write a parallel program based on a global name space by partitioning data and computation, generating message-passing function. In this paper, we describe the Parallel Programming Translator(PPTran), source-to-source data parallel compiler, generating MPI SPMD parallel program from HPF input program through four phases such as data dependence analysis, partitioning data, partitioning computation, and code generation with explicit message-passing and verify the performance of PPTran

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.22 no.4
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• pp.359-365
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• 2004

Data integration techniques are becoming increasing1y important for conquering a limitation with a single data. Image fusion which improves the spatial and spectral resolution from a set of images with difffrent spatial and spectral resolutions, and image registration which matches two images so that corresponding coordinate points in the two images correspond to the same physical region of the scene being imaged have been researched. In this paper, we compared with six image fusion methods(Brovey, IHS, PCA, HPF, CN, and MWD) with panchromatic and multispectral images of IKONOS and developed the registration method for applying to SPOT-5 satellite image and RADARSAT SAR satellite image. As the result of tests on image fusion and image registration, we could find that MWD and HPF methods showed the good result in term of visual comparison analysis and statistical analysis. And we could extract patches which depict detailed topographic information from SPOT-5 and RADARSAT and obtain encouraging results in image registration.

• Journal of the Society of Naval Architects of Korea
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• v.52 no.1
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• pp.19-24
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• 2015

The economic efficiency for fishery products can be related to their freshness and the quality. In order to freshly storage the product, freezing equipment system is necessary for fishing vessels. For this purpose, the horizontal plate freezer (HPF) is mostly used. In this research, our major objective is to improve the freezing performance of the HPF. Therefore, it is important to analyse the relationship between the shape of channels and the cooling temperature of the HPF. In order to save calculation time while checking the trend analysis between shape and performance, we used scaled models, and evaluate the cooling temperature of full scale model based on trend analysis results. The produced HPF in domestic was used, and the same operating conditions are considered. Based on this paper, the future research will be a comparison and verification through the experiments.

• Environmental Analysis Health and Toxicology
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• v.25 no.3
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• pp.241-251
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• 2010

Crucian carp (Carassius auratus) has been used as the sentinel species for POPs (Persistent Organic Pollutants) monitoring in aquatic environment. However, there is little information for dioxin toxicity and especially, early life stage toxicity in crucian carp have been never carried out. In this study, we investigated several toxic effects for 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in fertilized egg obtained by natural fertilization from crucian carp. The embryos at 3 h post-fertilization (hpf) were treated with 0.039, 0.156, 0.625, and 2.5 (${\mu}g/L$) TCDD by waterborne exposure for 60 minutes and changed with fresh water 2 times per day. Fertilized eggs started hatching at 51 hpf and TCDD exposed embryo showed decrease of hatching rate in a dose-dependent manner at 75 hpf. Pericardial edema was continuously observed in larvae exposed to TCDD from hatching start time (51 hpf), followed by the onset of mortality. In addition, AhR-related gene, CYP1A was clearly increased by TCDD in a dose dependent manner. These results indicated that fertilized eggs obtained from crucian carp have the TCDD related gene regulation and a distinct TCDD developmental toxicity syndrome by TCDD exposure. Therefore, we suggested that early life stage test in crucian carp could be used as test methods on dioxins toxicity.

• Korean Journal of Remote Sensing
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• v.14 no.2
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• pp.149-164
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• 1998

The main object of this study was to prove the effectiveness of different merging methods by using the high resolution IRS(Indian Remote Sensing Satellite)-1C panchromatic data and the multispectral Landsat TM data. The five methods used to merging the information contents of each of the satellite data were the intensity-hue-saturation(IHS), principal component analysis(PCA), high pass filter(HPF), ratio enhancement method and look-up-table(LUT) procedures. Two measures are used to evaluate the merging method. These measures include visual inspection and comparisons of the mean, standard deviation and root mean square error between merged image and original image data values of each band. The ratio enhancement method was well preserved the spectral characteristics of the data. From visual inspection, PCA method provide the best result, HPF next, ratio enhancement, IHS and LUT method the worst for the preservation of spatial resolution.

• The Journal of Korean Institute of Information Technology
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• v.17 no.8
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• pp.11-19
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• 2019

Against advanced image-related crimes, a high level of digital forensic methods is required. However, feature-based methods are difficult to respond to new device features by utilizing human-designed features, and deep learning-based methods should improve accuracy. This paper proposes a deep learning model to identify camera models based on DenseNet, the recent technology in the deep learning model field. To extract camera sensor features, a HPF feature extraction filter was applied. For camera model identification, we modified the number of hierarchical iterations and eliminated the Bottleneck layer and compression processing used to reduce computation. The proposed model was analyzed using the Dresden database and achieved an accuracy of 99.65% for 14 camera models. We achieved higher accuracy than previous studies and overcome their disadvantages with low accuracy for the same manufacturer.

• Journal of Yeungnam Medical Science
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• v.39 no.1
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• pp.39-45
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• 2022

Background: The current guidelines for the diagnosis of acute pyelonephritis (APN) recommend that APN be diagnosed based on the clinical features and the presence of pyuria. However, we observed that some of the patients who are diagnosed with APN do not have characteristic clinical features or pyuria at the initial examination. We performed this study to investigate the characteristics of APN without pyuria. Methods: A retrospective, cross-sectional study was conducted on 391 patients diagnosed with APN based on clinical and radiologic findings, between 2015 and 2019. The clinical features, laboratory results, and computed tomography (CT) findings were compared between patients with normal white blood cell (WBC) counts and those with abnormal WBC counts (WBC of 0-5/high power field [HPF] vs. >5/HPF) in urine. Results: More than 50% of patients with APN had no typical urinary tract symptoms and one-third of them had no costovertebral angle (CVA) tenderness. Eighty-eight patients (22.5%) had normal WBC counts (0-5/HPF) on urine microscopy. There was a negative correlation between pyuria (WBC of >5/HPF) and previous antibiotic use (odds ratio, 0.249; 95% confidence interval, 0.140-0.441; p<0.001), and the probability of pyuria was reduced by 75.1% in patients who took antibiotics before visiting the emergency room. Conclusion: The diagnosis of APN should not be overlooked even if there are no typical clinical features, or urine microscopic examination is normal. If a patient has already taken antibiotics at the time of diagnosis, imaging studies such as CT should be performed more actively, regardless of the urinalysis results.

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.570-578
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• 2023

Experiments on zebrafish embryo toxicity and whitening efficacy, which is an alternative experimental animal model, were conducted using coffee by-products. As a result of the embryo toxicity test treated with the coffee residue extract, the coagulation rate was 3, 3, and 5% at 24, 48, and 72 hpf and concentration of 125 ppm, respectively. The hatching rate of embryos was 73% at the highest concentration of 125 ppm. In the heart beat rate experiment of zebrafish larva, the heart beat rate after 72 hpf was confirmed to be 153 times/60 s' at a concentration of 125 ppm. The negative control group showed no significant change in heart rate compared to the control group at 148 times/60s', and showed low toxicity. In addition, as a result of evaluating the whitening effect in zebrafish, melanin formation was inhibited as the concentration of the coffee residue extract increased. The results of this study suggest the possibility that naturally derived by-product materials can be used as raw materials for cosmetics, and are expected to be used in the cosmetics industry as an example of research that increases the added value of natural product residue.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.1
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• pp.45-50
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• 2011

Hot-Press-Forming (HPF), an advanced sheet metal forming method using stamping at a high temperature of about $900^{\circ}C$ and quenching in an internally cooled die set, is one of the most successful forming process in producing crash-resistant parts such as pillars and bumpers with complex shape, ultrahigh strength, and minimum springback. To optimize conditions of a forming quality in HPF process and secure a safe product without any failures, such as fractures and wrinkling, the simulations based on the coupled thermo-mechanical analysis for a hot-press-formed lower control arm are applied with Taguchi's orthogonal array experiment. Three factor variables - the friction coefficient, blank shape, and hole location for burring - are selected to be optimized. The most effective condition of a forming quality for a hot-press-formed lower control arm is suggested. The simulation results are confirmed with experimental ones.