• Title, Summary, Keyword: HPTLC

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Constructing Database for Drugs and its Application to Biological Sample by HPTLC and GC/MS (HPTLC와 GC/MS를 이용한 의약품의 데이타베이스화 및 생체시료에의 응용)

  • Yoo, Young-Chan;Park, Sung-Woo;Lim, Mie-Ae;Baeck, Seung-Kyung;Park, Seh-Youn;Lee, Ju-Seon;Lho, Dong-Seok
    • Analytical Science and Technology
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    • v.13 no.2
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    • pp.136-150
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    • 2000
  • For the identification of unknown drugs in biological samples, we attempted rapid high performance thin layer chromatographic method which is sensitive and selective chromatographic analysis of high performance thin layer chromatography (HPTLC) with automated TLC sampler and ultra-violet (UV) scanner. We constructed HPTLC database (DB) on two hundred five drugs by using the data of Rf values and UV spectra (scan 200-360 nm) as well as gas chromatography/mass spectrometry (GC/MS) DB on ninety six drugs by using the data of relative retention time (RRT) on lidocain and mass spectra. After extracting drugs in biological sample by solid phase extraction (Clean Screen ZSDAU020), we applied them to HPTLC and GC/MS DB. Drugs, especially extracted from biological samples, showed good matching ratio to HPTLC DB and these drugs were confirmed by GC/MS. In conclusion, this DB system is thought to be very useful method for the screening of unknown drugs in biological samples.

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The Application of High Performance Thin Layer Chromatography for Herbal Formula Standardization (한약처방의 표준화를 위한 HPTLC의 활용)

  • Choi, Min-Kyung;Kim, Hyeong-Geug;Wang, Jing-Hua;Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.32 no.4
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    • pp.68-74
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    • 2011
  • Objective: The purpose of this study is to expatiate on high performance thin layer chromatography (HPTLC) as a simple, easy and scientific method for evaluation and standardization of herbal formulae. Methods: Through retrieving HPTLC application for herbal formulae in the literatures, the current situation of HPTLC application, and potential as well as limitation of HPTLC as a standardization method for multi-herbal drug was studied. Results: HPTLC is a speedy, inexpensive and well-operable tool for possessing multi-capability on component identification, separation, quantification and purification compared to other methods, such as high performance liquid chromatography (HPLC), gas chromatography (GC). Conclusions: HPTLC is considered as an available and convenient method for quality control and standardization of multi-herbal drugs. Thereby, this method could be recommended to widely applicate in the traditional Korean medicine.

A study on the screening of toxic materials by HPTLC and GC/MS (HPTLC 및 GC/MS를 이용한 유해화학물질의 스크리닝에 관한 연구)

  • Park, Sung-Woo;Jang, Seong-Gil;Park, You-Sin;Lee, Jin-Hoon;Lee, Sang-Ki;You, Jae-Hoon;Kim, Dong-Hwan;Jin, Kwang-Ho;Kim, Ki-Wook;Kim, Yu-Na;Lho, Dong-Seok
    • Analytical Science and Technology
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    • v.13 no.1
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    • pp.108-120
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    • 2000
  • To perform an effective screening for toxic materials of forensic interest detected in high profile criminal case in biological and environmental samples, we tried to construct a searchable computerized database using HPTLC(High Performance Thin Layer Chromatography) and GC/MS. Retardation factor($R_f$) values and UV spectral data of HPTLC were investigated for 160 pesticides, 34 chemicals and 39 explosives of standard grade. The data were compiled in a library. We also analyzed 112 pesticides, 31 chemicals and 17 explosives and 57 volatile organic compounds(VOCs) by GC/MS. The data for RT and characteristic mass ions were also compiled in a library.

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Normalization and Search of the UV/VIS Spectra Measured from TLC/HPTLC (TLC/HPTLC에서 측정된 자외/가시부 스펙트럼의 표준화 및 검색)

  • Kang, Jong-Seong
    • YAKHAK HOEJI
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    • v.38 no.4
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    • pp.366-371
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    • 1994
  • To improve the identification power of TLC/HPTLC the in situ reflectance spectra obtained directly from plates with commercial scanner are used. The spectrum normalization should be carried out prior to comparing and searching the spectra from library for the identification of compounds. Because the reflectance does not obey the Lambert-Beer's law, there arise some problems in normalization. These problems could be solved to some extent by normalizing the spectra with regression methods. The spectra are manipulated with the regression function of a curve obtained from the correlation plot. When the parabola was used as the manipulating function, the spectra were identified with the accuracy of 97% and this result was better than that of conventionally used the point and area normalization method.

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Quantitation of abamectin by HPTLC and its pharmacokinetics after intramuscular injection in pigs (돼지에서 근육주사한 Abamectin에 대한 HPTLC 분석 및 약물동태학)

  • Park, Seung-chun;Yun, Hyo-in
    • Korean Journal of Veterinary Research
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    • v.40 no.1
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    • pp.35-41
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    • 2000
  • We established a new method to analyze abamectin using HPTLC (high performance thin layer chromatography) in order to obtain its pharmacokinetic profiles in pigs. Recovery of abamectin in pig serum after fluorescence derivatization was $80.01{\pm}3.82%$ at 0.1ppm and $83.67{\pm}3.63%$ at 10ppm, respectively. Detection reproducibility in terms of coefficient variation (c.v.) was 3.09% and 2.74% (intra-day), and 3.71% and 51.7%(inter-day), for 0.1 and 10ppm, respectively. Pharmacokinetics of abamectin was studied in five Yorkshire-Landrace mixed bred male pigs ($35.0{\pm}2.7kg$) administered intramuscularly 0.3mg/kg b.w. Pharmacokinetic profiles of abamectin in pigs were described by the 1-compartment open model with first-order absorption and first-order elimination. AUC (area under the curve) was $262.65{\pm}16.44ng{\cdot}day/ml$ and the biological elimination half-life ($t_{1/2},\;k_e$) was $5.28{\pm}0.84$ days, indicating somewhat high bioavailability and long half-life by the intramuscular route. We suggest intramucular injection of abamectin could be also used in place of the recommended route of its subcutaneous administration so far.

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Isolation and Quantitative Analysis of Betulinic Acid and Alphitolic Acid from Zyziphi Fructus (대추로부터 베튜리닉 산과 알피톨릭 산의 분리 및 정량)

  • Bae, Gi-Hwan;Lee, Sang-Myeong;Lee, Eun-Sil;Lee, Jun-Seong;Gang, Jong-Seong
    • YAKHAK HOEJI
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    • v.40 no.5
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    • pp.558-562
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    • 1996
  • Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase column (Nova-Pak C18) with 0.05M Na2HPO4-methanol (19:81) in HPLC and detected at 210nm. Separation on HPTLC precoated silica gel F254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with p-anisaldehyde and detected at 540nm. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.

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Pharmacognostic Evaluation of Pipper longum Linn. Fruit

  • Gupta, Meenu;Srivastava, Sharad;Mehrotra, Shanta;Sharma, Vinita;Rawat, AKS;Srivastava, Manjoosha
    • Natural Product Sciences
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    • v.13 no.2
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    • pp.97-100
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    • 2007
  • The present study includes macro and microscopical details, powder study, physico-chemical study and HPTLC fingerprinting of the Piper longum fruits. Microscopic studies revealed the presence of stone cells, starch grains and thin walled fragments of parenchymatous cells. Physico-chemical studies showed alcohol and ether soluble extract 24.53 and 6.7, sugar 0.35, starch 21.33 and tannin 0.83% respectively. Successive soxhlet extract showed maximum percentage of hexane soluble fraction i.e. 22.52. The HPTLC profile has also been performed against the reference marker pipeline, which was identified at R$_f$ 0.42. In the present paper a detailed pharmacognostical evaluation of fruit has been undertaken.

Pharmacognostic Evaluation of Curcuma caesia Roxb. rhizome

  • Verma, Durgesh;Srivastava, Sharad;Singh, Vineet;Rawat, A.K.S.
    • Natural Product Sciences
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    • v.16 no.2
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    • pp.107-110
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    • 2010
  • Curcuma caesia Roxb. (Zingiberaceae) is commonly known as 'Black turmeric'. In India it grows in West Bengal, Madhya Pradesh, Orissa, Bihar, North-East and Uttar Pradesh and is widely used by ethnic communities for various ailments. Rhizomes of the plant are used for sprains and bruises and are also employed in cosmetics. In West Bengal it is an important place in traditional system of medicine and is also used as a substitute for turmeric in fresh stage. Present communication deals with the detailed pharmacognostical evaluation of the rhizome sample. Inner part of the rhizome is bluish-black in colour and emits a characteristic sweet smell, due to the presence of essential oil. On steam distillation the rhizome yields an essential oil rich in camphor. A detailed HPTLC studies has been carried out for quantitative evaluation of active marker component. HPTLC, physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of C. caesia rhizome and may possibly help to differentiate the drug from its other allied species.

Pharmacognostical Evaluation and Phytochemical Standardization of Abrus precatorius L. Seeds

  • Verma, Durgesh;Tiwari, Shashi Shankar;Srivastava, Sharad;Rawat, A.K.S.
    • Natural Product Sciences
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    • v.17 no.1
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    • pp.51-57
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    • 2011
  • The seeds of Abrus precatorius L. (Family- Fabaceae) constitute the drugs Abrus, Gunja, or Ratti in commerce. In the Indian System of Medicine, the seeds are used for sciatica, paralysis, headache, dysentery, diarrhoea, leprosy, ulcer, nervous disorders, alopecia, as well as anti-inflammatory, antidiabetic, antibacterial, antitumor, sexual stimulant and abortifacient. Seeds are poisonous and therefore are used after mitigation. The protein abrin is responsible for the highly toxic properties of seeds. Quantitative HPTLC analysis of the methanolic extract of seeds determined the presence of 0.4018% gallic acid and 0.4009% glycyrrhizin. The present study was undertaken to develop an HPTLC method, as well as ascertain the physico-chemical, morphological and histological parameters to establish the authenticity of A. precatorius seeds.