• Title/Summary/Keyword: Hazel

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Studies on the Processing Adaptability of Hazel Nut - Separation of Protein from Defatted Hazel Nut Meal and Characterization of the Protein Isolates - (개암종실(種實)의 가공적성(加工適性)에 관(關)한 연구(硏究) - 개암종실(種實) 탈지박(脫脂粕)의 단백질(蛋白質) 분리(分離) 및 분리단백질(分離蛋白質)의 특성(特性)에 대하여)

  • Yoon, Han Kyo;Keum, Jong Wha;Lee, Jong Soo;Oh, Man Jin
    • Korean Journal of Agricultural Science
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    • v.8 no.2
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    • pp.231-237
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    • 1981
  • In order to obtain the basical data for utilization of protein in hazel nut, protein was extracted from defatted hazel nut meal with salt solutions and alkaline solutions, and precipitated by adjusting pH of extract to 5.5 or addition of organic solvents. Amino acid composition of the isolated protein and defatted hazel nut meal were analyzed, protein isolates were identified by polyacrylamide gel electrophoresis. The results summarized were as follows. 1. Defatted hazel nut showed highly nutritional value as the content of 'protein was 53.6%. 2. Extractabilities of salt-soluble protein treated with 2.5M $MgCl_2$, and 1M NaCl(pH 11.0) were 53.0%, 31.5%, respectively 3. Protein in hazel nut were contained 53% of salt-soluble globulin, 14% of water-soluble albumin, 29.5% of glutelin based on solubility. 4. At pH 5.5, 85% of the extracted protein was precipitated, and about 90% of the extracted protein was separated by addition of organic solvents such as acetone and ethanol at 60-70% concentration. 5. Proteins extracted from defatted hazel nut with water and 0.027N NaOH showed 3 and 6 hands by polyacrylamide gel electrophoresis, respectively. 6. Amino acids of defatted hazel nut and protein isolate were chiefly composed of glutamic acid, arginine and aspartic acid.

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Studies on the Neutral Lipid Components of Korean Hazel Nut (한국산 개암종실의 중성지질 조성에 관한 연구)

  • 김미란;고영수;정보섭
    • YAKHAK HOEJI
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    • v.25 no.4
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    • pp.181-186
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    • 1981
  • Compositions of neutral lipid of the hazel nut (Corylus heterophylla Fischer var. thumbergii Blume, Betulaceae) produced in Korea were analyzed by thinchrography (Iatrosoan TH-10 type), which was equipped with a flame ionization detector. The neutral lipid components were nearly separated of the basis of their degree of unsaturation by employing the chromatography on silica gel sintered rod impregnated with 12.5% silver nitrate and it was noted that triglyceride was the major lipid components of Korean hazel nut. The triglyceride compositions were trilinolenin 10.63, trilinolein 18.01, triolein 42.24, tripalmitin 17.57 and tristearin 11.55% by thinchrographic analysis.

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Studies on the Content of Triacylglycerol Species, Tocopherols, and Phytosterols from the Selected Nuts (견과류의 지방산, 트리아실글리세롤, 토코페롤 및 파이토스테롤의 조성 연구)

  • Sung, Min-Hye;Lyu, Hyun-Kyeong;Lee, Sun-Mo;Lee, Ki-Teak
    • Food Science and Preservation
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    • v.17 no.3
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    • pp.376-383
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    • 2010
  • Including crude fat content, triacylglycerol species, tocopherols and phytosterols were analyzed in 8 kinds of nuts (sunflower seed, cashew nut, walnut, pistachio, pumpkin seed, ginkgo, hazel nut and pecan). The extracted crude fats showed 0.63~39.60 wt%, among which hazel nut showed the highest amount of fat content. Oleic acid (C18:1) was major fatty acids at sn-2 position in cashew nut, pistachio, hazel nut, and pecan while sunflower seed, walnut, and pumpkin seed showed linoleic acid (C18:2) as a major fatty acids at sn-2 position. Especially, ginkgo contained 10.72 wt% of vaccenic acid (C18:1-n7) at sn-2 position. The TAG species of 8 kinds of nuts were analyzed by reverse-phase HPLC, from which PN value ranged 40~52. Among the analyzed nuts, higher content of tocopherols were observed in ginkgo (48.57 mg/100 g), sunflower seed (38.35 mg/100 g), and pumpkin seed(31.43 mg/100 g). Total phytosterols were observed with the range of 88.60~947.20 mg/100 g.

Studies on the Amino Acid Components of Korean Hazel Nut (한국산(韓國産) 개암 종실(種實)의 아미노산 조성(組成)에 관한 연구)

  • Kim, Mi-Ran;Ko, Young-Su
    • Korean Journal of Food Science and Technology
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    • v.13 no.1
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    • pp.1-5
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    • 1981
  • Free and total amino acids of Korean hazel nut (Corylus heterophylla Fisch. var. Japonica koidz.) were identified by amino acid autoanalyzer (Technicon PNC-1 type). The results indicate that among 16 kinds of all free amino acids contained in hazel nut, arginine was the richest, and then came glutamic acid, proline, alanine, tyrosine and valine in that order. From the total amino acids which were closely related with the nutritional valuation, arginine was in the richest amount and then came with glutamic acid, proline, leucine, aspartic acid and valine.

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Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway)

  • Wang, Yechun;Guo, Binhui;Zhang, Fei;Yao, Hongyan;Miao, Zhiqi;Tang, Kexuan
    • BMB Reports
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    • v.40 no.6
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    • pp.861-869
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    • 2007
  • The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of CgHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that CgHMGR belonged to a small gene family. Expression analysis revealed that CgHMGR expressed high in roots, and low in leaves and stems, and the expression of CgHMGR could be up-regulated by methyl jasmonate (MeJA). The functional color assay in Escherichia coli showed that CgHMGR could accelerate the biosynthesis of $\beta$-carotene, indicating that CgHMGR encoded a functional protein. The cloning, characterization and functional analysis of CgHMGR gene will enable us to further understand the role of CgHMGR involved in taxol biosynthetic pathway in C. avellana at molecular level.

A Study on the Lipid Components of Hazel Nut Oil (개암종실(種實)의 지질성분(脂質成分)에 관한 연구)

  • Hong, Hyung-Ki;Shin, Hyo-Sun
    • Korean Journal of Food Science and Technology
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    • v.10 no.3
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    • pp.361-365
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    • 1978
  • Physico-chemical characteristics of crude oil extracted from Korean Hazel nut were determined and its proximate composition was also analyzed. The proximate composition of Hazel nut was shown to be moisture 4.0%, crude protein 15.5%, crude fat 64%, nitrogen free extractive 11.7%, crude fiber 2.0% and crude ash 2.5%. The content of crude fat in Corylus sieboldiana was about 3% higher than in Corylus mandshurica.. Physico-chemical characteristics of crude oil found were: specific gravity, $0.916(15/15^{\circ}C)$; refractive index, $1.468(15^{\circ}C)$; saponification value, 184; iodine value, 94.5: acid value, 0.2; and unsaponifiable content, 0.25%. The lipid fractions in the crude oil obtained by silicic acid column chromatography were found to be composed of about 97% neutral lipids and about 3% compound lipids. Among the neutral lipids by TLC, triglycerides were 98% as the major components, free fatty acids and free strols were 0.5% and 1.3%, respectively. Esterified sterols were not found. The predominant fatty acids were oleic $(76{\sim}80%)$, linoleic (15%) and palmitic (5.0%), and the P/S ratio was $1.8{\sim}2.8$.

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Management of severe hidradenitis suppurativa with biologic therapy and wide excision

  • Lim, Shi Yu Derek;Cheong, Ee Cherk;Oon, Hazel H.
    • Archives of Plastic Surgery
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    • v.46 no.3
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    • pp.272-276
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    • 2019
  • Hidradenitis suppurativa (HS) is a chronic inflammatory follicular occlusive disease that involves the intertriginous areas. Treatment methods include conventional topical and systemic medication, radiotherapy, biologic agents, and surgical excision. Of late, there has been an increased focus on the use of biologic agents in patients with moderate to severe HS. Here, we present the case of a 46-year-old man with Hurley stage III HS for whom wide excision was ultimately curative, after aggressive medical therapy with the use of infliximab and adalimumab had succeeded in limiting the body surface area affected by the disease. This case demonstrates the effective treatment of severe HS with a combination of biologic therapy and surgery.

Mycorrhization of Quercus spp. with Tuber huidongense and T. himalayense Collected in Korea

  • Gwon, Ju-Hui;Park, Hyeok;Eom, Ahn-Heum
    • Mycobiology
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    • v.50 no.2
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    • pp.104-109
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    • 2022
  • Fungi of the genus Tuber are ectomycorrhizal fungi that form a symbiotic relationship mainly with oak and hazel trees. Tuber spp. exhibit a highly selective host plant preference; thus, for cultivation purposes it is important to select an appropriate host plant for successful mycorrhization. In addition, as mycorrhizal characteristics differ according to Tuber spp., it is necessary to understand the differences in mycorrhizae according to the fungal species. Tuber huidongense and Tuber himalayense were recently discovered in Korea; therefore, we used spore suspensions from these two species to inoculate two species of oak trees, Quercus acutissima and Quercus dentata, to compare colonization rates and morphologies of the mycorrhizae. The colonization rates demonstrated that the different Tuber spp. favored different host plant species. In addition, unique morphological and anatomical characteristics were observed for T. huidongense and T. himalayense depending on the host species. These findings can lead to new economically important agricultural activities related to truffle cultivation in Korea.