• Food Science of Animal Resources
• /
• v.26 no.4
• /
• pp.464-470
• /
• 2006

This study was carried out to investigate the effect of various high pressure freezing treatments on the physical properties of pork To compare the effect of freezing on meat quality, atmospheric freezing (AF), pressure and freezing (PF), pressure shift freezing (USF) and pressure assisted freezing (PAF) were conducted at pressure of 100 MPa. Water binding properties, shear force and colour were measured as physical properties of pork PAF showed shorter phase transition time than PSF. Although significant increase (p<0.05) in water binding properties was found only at PAF, meat frozen under hydrostatic pressure environment showed improved water binding properties. However, all high pressure freezing treatment caused significantly increased shear force (p<0.05), especially at PF treatment. In color, all high pressure freezing treatments showed significantly higher color parameters (p<0.05) than AF, whilst no significant differences among high pressure freezing treatments were found (p>0.05). Therefore the result indicated that applied hydrostatic pressure improved functional properties of pork with increasing freezing rate and PAF had more potential benefit than PSF at mild pressure range.

• Food Science of Animal Resources
• /
• v.36 no.3
• /
• pp.335-342
• /
• 2016

The object in this study is to investigate the effects of high pressure and freezing processes on the curdling of skim milk depending on the presence of transglutaminase (TGase) and glucono-δ-lactone (GdL). Skim milk was treated with atmospheric freezing (AF), high pressure (HP), pressure-shift freezing (PSF) and high pressure sub-zero temperature (HPST) processing conditions. After freezing and pressure processing, these processed milk samples were treated with curdling agents, such as TGase and GdL. Pressurized samples (HP, PSF and HPST) had lower lightness than that of the control. In particular, PSF had the lowest lightness (p<0.05). Likewise, the PSF proteins were the most insoluble regardless of whether they were activated by TGase and GdL, indicating the highest rate of protein aggregation (p<0.05). Furthermore, the TGase/GdL reaction resulted in thick bands corresponding to masses larger than 69 kDa, indicating curdling. Casein bands were the weakest in PSF-treated milk, revealing that casein was prone to protein aggregation. PSF also had the highest G' value among all treatments after activation by TGase, implying that PSF formed the hardest curd. However, adding GdL decreased the G' values of the samples except HPST-treated samples. Synthetically, the PSF process was advantageous for curdling of skim milk.

• Food Science of Animal Resources
• /
• v.29 no.6
• /
• pp.736-742
• /
• 2009

This study was conducted to investigate the effect of various high pressure freezing and thawing treatments on the physical properties of pork. To compare the effects of the freezing and thawing process on meat quality, atmospheric freezing followed by running water thawing (AFRT), pressure shift freezing followed by running water thawing (SFRT), and pressure shift freezing and pressure assisted thawing (SFAT) were conducted at pressure of 250 MPa and cooling temperature of $-22^{\circ}C$. SAFT and SFRT showed a shorter phase transition time and total thawing time than AFRT. The pH value of treated samples increased significantly (p<0.05) compared to unfrozen meat. In addition, SFAT and SFRT showed a higher pHvalue than AFRT. Although the water holding capacity was significantly decreased (p<0.05) for SFAT and SFRT, SFRT reduced drip loss. In regards to color, SFAT and SFRT resulted in a significant increase in color parameters (p<0.05) relative to AFRT, while SFAT produced a higher L*-value. High pressure treatment significantly increased shear force (p<0.05) compared to AFRT, and, where SFRT showed the highest shear force. Therefore, these combined results indicated that the hydrostatic pressure treatment improved the functional properties of pork and increased the freezing and thawing rate.

• Journal of Ocean Engineering and Technology
• /
• v.27 no.6
• /
• pp.106-111
• /
• 2013

In this study, numerical simulations and performance evaluation has been carried out to investigate performance characteristics of air-type high pressure piston pump. ANSYS and CFX were applied for analyzing the structure and flow behavior of air-type high pressure piston pump, respectively. The performance evaluation of high pressure piston pump was performed experimentally, the results were compared with simulation. It was found that the freezing phenomenon was improved by 20% and the pressure fluctuation decreased by 50%, compared with the previous pump.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.20 no.2
• /
• pp.255-268
• /
• 2018

Subsea tunnel can be highly vulnerable to seawater intrusion due to unexpected high-water pressure during construction. An artificial ground freezing (AGF) will be a promising alternative to conventional reinforcement or water-tightening technology under high-water pressure conditions. In this study, the freezing energy and required time was calculated by the theoretical model of the heat flow to estimate the total amount of refrigerant required for the artificial ground freezing. A lab-scale freezing chamber was devised to investigate changes in the thermal and mechanical properties of sandy soil corresponding to the variation of the salinity and water pressure. The freezing time was measured with different conditions during the chamber freezing tests. Its validity was evaluated by comparing the results between the freezing chamber experiment and the numerical analysis. In particular, the freezing time showed no significant difference between the theoretical model and the numerical analysis. The amount of refrigerant for artificial ground freezing was estimated from the numerical analysis and the freezing efficiency obtained from the chamber test. In addition, the energy ratio for maintaining frozen status was calculated by the proposed formula. It is believed that the energy ratio for freezing will depend on the depth of rock cover in the subsea tunnels and the water temperature on the sea floor.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.16 no.5
• /
• pp.431-443
• /
• 2014

In this paper case studies of artificial ground freezing, which have not been applied in Korea, have been investigated for the water cut-off in a subsea tunnel under high water pressure and the most commonly used cooling mediums of brine and liquid nitrogen are examined. Since sea water with pressure has the lower freezing point than pure water, the lower temperature cooling medium is required in the application of subsea tunnel. Also, the cooling medium must have refrigeration safety and is able to reduce executing time. Brine freezing system can reuse cooling medium and is safer than liquid nitrogen freezing. But it takes more time to freeze ground and needs complex circulation plants. On the other hand, liquid nitrogen freezing system can't recycle cooling medium and may cause breathing problems or asphyxiation through oxygen deficiency. But, freezing with liquid nitrogen is fast and requires simple refrigeration equipment. Principal elements of design for ground freezing in subsea tunnel have been extracted and these elements are needed further research.

• ALGAE
• /
• v.21 no.4
• /
• pp.479-483
• /
• 2006

In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.

• Journal of Advanced Marine Engineering and Technology
• /
• v.22 no.5
• /
• pp.680-686
• /
• 1998

In the adoption of a desalination system the most important factor is the cost of fresh water pro-duction. In general LNG is stored in a tank as a liquid state below $-162^{\circ}C$ When it is serviced however the LNG absorbs energy from a heat source and it is transformed to a high pressure gaseous state. During this process a huge amount of cold energy accumulated in cooling LNG is wasted. This wasted cold energycan be utilized to produce fresh water by using a sea water freez-ing desalination system. in order to develop a sea water freezing desalination system and to estab-lish its design technique qualitative and quantitative data regarding the freezing behavior of sea water is required in advance. The goals of this study are to reveal the freezing mechanisms of sea water in a cooled circular tube to measure the freezing rate and to investigate the freezing heat-transfer characteristics. The experimental results provide a general understanding of sea water freezing behavior in a cooled circular tube.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2005.10a
• /
• pp.136-141
• /
• 2005

In this research, PSF and PBF indicated beneficial influence on water binding ability of meat comparing to conventional freezing methods. High pressure in freezing process resulted some changes inhysical characteristics of pork: increment each color value, shear force and pH in PSF, PAF and PBF.

• Atmosphere
• /
• v.25 no.1
• /
• pp.31-49
• /
• 2015

From the hourly data of 75 Korean weather stations over a 12-year period (2001~2012), this study has chosen three cases (January 12, 2006; January 11, 2008; and February 22, 2009) of multiple freezing rains and investigated the atmospheric circulations that seemed to cause the events. As a result, the receding high pressure type (2006), prevailing high pressure type (2008), and warm front type (2009) are confirmed as synoptic patterns. In all three cases, freezing rain was found in regions with a strong ascending current near the end point of a low-level jet that carried the warm humid air from low latitudes. The strong ascending current resulted from lower-level convergence and upper-level divergence. In 2006 and 2009, the melting process was confirmed. In 2008, the supercooled warm rain process (SWRP) was confirmed. In contrast to existing SWRP theory, it was found that the cool air produced at the middle atmosphere and near the earth's surface led to the formation of freezing rain. The sources of this cool air were supposed to be the evaporative latent heat and the cold advection coming from the northeast. On the other hand, a special case was detected, in which the freezing rain occurred when both the soil surface temperature and surface air temperature were above $0^{\circ}C$. The thickness distributions related to freezing rain in Korea were found to be similar to those in North America. A P-type nomogram was considered for freezing rain forecasting; however, it was not relevant enough to Korea, and few modifications were needed.