• Title/Summary/Keyword: Hyaluronic acid

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An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis (Angelica gigas 에탄올 추출물의 Hyaluronic acid 합성 효과에 대한 실험적 연구)

  • Park, Hye-Su;Ha, Hun-Yong;Kim, Hee-Taek
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.31 no.1
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    • pp.32-41
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    • 2018
  • Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study is to examine the effects of Angelica gigas(A. gigas) ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There were no cytotoxicity in $50{\mu}g/ml$ concentration A. gigas extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration A. gigas extract. Hyaluronic acid production was higher than control group in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration A. gigas extract. Conclusions : Hyaluronic acid production was increased by A. gigas extracts. Therefore, We suggest that A. gigas can make a contribution to the moisturizing effect on human skin.

The effect of Astragalus membranaceus methanol extract on hyaluronic acid production in HaCaT cells (황기 메탄올 추출물이 HaCaT 세포에서 Hyaluronic acid 생성에 미치는 영향)

  • Lee, Pyeong-Jae;Kim, Hee-Taek;Yoon, Kyung-Sup;Park, Hyun-Chul;Ha, Hun-Yong
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.26 no.1
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    • pp.75-81
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    • 2013
  • Objectives: Hyaluronic acid, high molecular glycosaminoglycan, exists in extracellular matrix of tissue, especially, in skin and has been known to be deeply involved in skin hydration. In this study, we investigated the effect of methanol extract of Hwang-gi, Astragalus membranaceus root, on hyaluronic acid production in human keratinocyte HaCaT cells. Methods: We determined hyaluronic acid synthase 2 gene expression and hyaluronic acid production in HaCaT cells by using RT-PCR and ELISA, respectively. Results: Hwang-gi extract didn't show the toxicity to HaCaT cells within the treated concentration and increased the hyaluronic acid synthase 2 gene expression and hyaluronic acid production. Conclusions: Hyaluronic acid production increased by Hwang-gi could be, partially, contribute to the moisturing effect in skin by it.

An Experimental Study on the Effect of Angelica acutiloba Ethanol Extract on Hyaluronic Acid Synthesis (일당귀 에탄올 추출물의 Hyaluronic Acid 합성 효과에 대한 실험적 연구)

  • Kang, Min-Seo;Ha, Hun-Yong;Kim, Hee-Taek
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.28 no.1
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    • pp.32-40
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    • 2015
  • Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and anti-aging. The purpose of this study is to examine the effects of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There was no cytotoxicity in $50{\mu}g/ml$ concentration Angelica acutiloba extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration Angelica acutiloba extract. Hyaluronic acid production was higher in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration Angelica acutiloba extract than control group. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin.

Production of Hyaluronic Acid from Streptococcus zooepidemicus (Streptococus zooepidemicus에 의한 히아루론산의 생산)

  • 유대식
    • KSBB Journal
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    • v.7 no.2
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    • pp.112-117
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    • 1992
  • An optimal composition of medium for hyaluronic acid production and some characteristics of its from Streptococcus zooepidemicus were investigated. The hyaluronic acid from S. zooepidemicus was reached maximum level in the BY-medium containing 0.1% beef extract, 0.1% yeast extract, 3.0% glucose, 2.0% peptone, 0.1% NaCl and $0.5%CaCO_3$ (pH 7.5) at $37^{\circ}C$ for 36 hours with shaking. Addition of $CaCO_3$ to the medium was necessary to neulralize the lowered pH which was resulted from hyaluronic acid production. Molecular weights of extracelluar and cellular hyaluronic acid produced by the strain were $1-1.4{\times }10^6$ and $5{\times}10^6$, respectively. The amount of extracellular hyaluronic acid was 91.9% of total hyaluronic acid produced and the vest was all intracellular.

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Hyaluronidase: An overview of its properties, applications, and side effects

  • Jung, Hyunwook
    • Archives of Plastic Surgery
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    • v.47 no.4
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    • pp.297-300
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    • 2020
  • Hyaluronidase, an enzyme that breaks down hyaluronic acid, has long been used to increase the absorption of drugs into tissue and to reduce tissue damage in cases of extravasation of a drug. With the increasing popularity of hyaluronic acid filler, hyaluronidase has become an essential drug for the correction of complications and unsatisfactory results after filler injection. For this reason, when performing procedures using hyaluronic acid filler, a sufficient knowledge of hyaluronidase is required. In order for hyaluronidase to dissolve a hyaluronic acid filler, it must interact with its binding sites within the hyaluronic acid. The reaction of a filler to hyaluronidase depends on the hyaluronic acid concentration, the number of crosslinks, and the form of the filler. Hyaluronidase is rapidly degraded and deactivated in the body. Therefore, in order to dissolve a hyaluronic acid filler, a sufficient amount of hyaluronidase must be injected close to the filler. If the filler is placed subcutaneously, injection of hyaluronidase into the filler itself may help, but if the filler is placed within a blood vessel, it is sufficient to inject hyaluronidase in the vicinity of the vessel, instead of into the filler itself. Allergic reactions are a common side effect of hyaluronidase. Most allergic reactions to hyaluronidase are local, but systemic reactions may occur in infrequent cases. Since most allergic responses to hyaluronidase are immediate hypersensitivity reactions, skin tests are recommended before use. However, some patients experience delayed allergic reactions, which skin tests may not predict.

Physiochemical and Organoleptic Properties of Kefir Containing Different Concentrations of Hyaluronic Acid : A Preliminary Study

  • Chon, Jung-Whan;Kim, Binn;Seo, Kun-Ho;Bae, Dongryeoul;Jeong, Dongkwan;Song, Kwang-Young
    • Journal of Dairy Science and Biotechnology
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    • v.38 no.3
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    • pp.146-153
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    • 2020
  • Recently, hyaluronic acid, which is the most popular among various biomaterials, has been used in osteoarthritis treatment, eye surgery, and wound healing. This study was carried out to determine the physicochemical and organoleptic properties of kefir containing various concentrations of hyaluronic acid. The pH value of hyaluronic-acid-containing kefir ranged from 3.58 to 3.46. Moreover, for hyaluronic-acid-containing kefir, the score of taste ranged from 4.75 to 4.0, the flavor ranged from 4.0 to 3.38, the color ranged from 4.38 to 4.0, the texture ranged from 4.38 to 3.75, and the overall acceptability ranged from 4.59 to 3.72. Overall, the organoleptic properties of hyaluronic-acid-containing kefir were better than those of the control. Because this is the first study involving hyaluronic acid and kefir, the results are valuable. The addition of hyaluronic acid did not significantly affect the physicochemical and organoleptic properties of kefir. Therefore, the commercial availability of various hyaluronic-acid-added dairy food products must be investigated.

Purification and Characterization of High Viscous Hyaluronic Acid Complex from Klebsiella sp. L-10 NTG 50 (Klebsiella sp. L-10 의 NTG 50 변 이주로부터 생산된 고점성 히알우론산 복합체의 정제 및 특성)

  • 이향숙;김나미
    • The Korean Journal of Food And Nutrition
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    • v.9 no.3
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    • pp.242-246
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    • 1996
  • High viscous hyaluronic acid complex from Klebsiella sp. L-10 NTG 50 mutant was purified by two-phase extraction system using PEG-K2HP04 and its physicochemical properties were Investigated. Viscosity of the purified hyaluronic acid complex was decreased as temperature and salts concentration were Increased and also showed low viscosity at below pH 5.0 and above pH 11.0. Hardness, cohesiveness and adhesiveness of the purified hyaluronic acid complex were 1, 20kg, 1.91 and 0.62, respectively. Water holding capacity was 6.9ml per gram of the purified hyaluronic acid complex powder.

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A Case of Nonthrombotic Pulmonary Embolism after Facial Injection of Hyaluronic Acid in an Illegal Cosmetic Procedure

  • Jang, Jong Geol;Hong, Kyung Soo;Choi, Eun Young
    • Tuberculosis and Respiratory Diseases
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    • v.77 no.2
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    • pp.90-93
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    • 2014
  • Hyaluronic acid is widely used in medical procedures, particularly in cosmetic procedures administered by physicians or nonmedical personnel. The materials used for cosmetic procedures by physicians as well as illegally by non-medical personnel can cause nonthrombotic pulmonary embolism (NTPE). We report the case of a woman with acute respiratory failure, neurologic symptoms and petechiae after an illegal procedure of hyaluronic acid dermal filler performed by an unlicensed medical practitioner 3 days before symptom onset. Although a few cases of NTPE after injection of hyaluronic acid have been reported yet, this is the first typical case showing a NTPE manifestation after the facial injection of hyaluronic acid.

Hyaluronic Acid and Platelet-Rich Plasma Injections in Foot and Ankle Disorders (족부족관절 질환에서 히알루론산과 혈소판 풍부 혈장 주사치료)

  • Gun-Woo Lee;Joonkyoo Kang
    • Journal of Korean Foot and Ankle Society
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    • v.27 no.3
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    • pp.79-86
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    • 2023
  • Numerous nonoperative treatments have been used to reduce pain and improve the clinical outcomes of patients with foot and ankle disorders. Among these treatments, hyaluronic acid and platelet-rich plasma (PRP) injections have been used increasingly. This review analyzes the recent literature reporting the efficacy and indications for hyaluronic acid and PRP injections. Hyaluronic acid and PRP can be used safely as adjuncts. Hyaluronic acid injections have shown efficacy and have a promising role as an adjuvant treatment method. In addition, its indication has expanded to various foot and ankle diseases over osteoarthritis. On the other hand, the efficacy of PRP injection has not yet been clarified, and the lack of standardization of procedures for the preparation and administration of PRP makes it difficult to establish definitive treatment indications.

Purification of Biosynthesized Hyaluronic Acid for Its Medical Application (생합성 히아루론산의 의료용을 위한 정제)

  • 오덕근
    • KSBB Journal
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    • v.11 no.1
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    • pp.15-21
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    • 1996
  • Purification of hyaluronic acid produced by Streptococcus equi was carried out to obtain clinical grade hyaluronic acid. The removal method of the bacteria was selected as filtration because filtration was the most effective method in removing impurities such as protein and nucleic acid of the fermentation broth. The removal efficiencies of protein and nucleic acid of hyaluronic acid solution were increased to 75% and 67%, respectively, by filtration with adding 0.6% of activatied carbon and 1.0% colite. Hyaluronic acid solution was precipitated by mixing with 2 volumes of ethanol. Effects of pH and conductivity on ethanol preciptation of hyaluronic acid were investigated. Protein and nucleic acid of hyaluronic acid were remained almost constant regardless of pH and conductivity, and the recovery of hyaluronic acid was optimum as about 85% at pH 7 and l00mS of conductivity Protein of hyaluronic acid was completly removed by three serial filtration and ethanol precipitation, however, nucleic acid was not removed. Hyaluronic acid solution was passed through a column of Duolite A7 to remove its nucleic acid, where 65% of nucleic acid was removed at pH 7 and 40mS of conductivity. The residual nucleic acid of hyaluronic acid solution was completly removed by treatment of 0.2% hydroxyapatite and the clinical grade hylauronic acid could be obtained.

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