• 한방안이비인후피부과학회지
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• 제31권1호
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• pp.32-41
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• 2018

Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study is to examine the effects of Angelica gigas(A. gigas) ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There were no cytotoxicity in $50{\mu}g/ml$ concentration A. gigas extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration A. gigas extract. Hyaluronic acid production was higher than control group in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration A. gigas extract. Conclusions : Hyaluronic acid production was increased by A. gigas extracts. Therefore, We suggest that A. gigas can make a contribution to the moisturizing effect on human skin.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.75-81
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• 2013

Objectives: Hyaluronic acid, high molecular glycosaminoglycan, exists in extracellular matrix of tissue, especially, in skin and has been known to be deeply involved in skin hydration. In this study, we investigated the effect of methanol extract of Hwang-gi, Astragalus membranaceus root, on hyaluronic acid production in human keratinocyte HaCaT cells. Methods: We determined hyaluronic acid synthase 2 gene expression and hyaluronic acid production in HaCaT cells by using RT-PCR and ELISA, respectively. Results: Hwang-gi extract didn't show the toxicity to HaCaT cells within the treated concentration and increased the hyaluronic acid synthase 2 gene expression and hyaluronic acid production. Conclusions: Hyaluronic acid production increased by Hwang-gi could be, partially, contribute to the moisturing effect in skin by it.

• 한방안이비인후피부과학회지
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• 제28권1호
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• pp.32-40
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• 2015

Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and anti-aging. The purpose of this study is to examine the effects of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There was no cytotoxicity in $50{\mu}g/ml$ concentration Angelica acutiloba extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration Angelica acutiloba extract. Hyaluronic acid production was higher in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration Angelica acutiloba extract than control group. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin.

• KSBB Journal
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• 제7권2호
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• pp.112-117
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• 1992

Streptococcus zooepidemicus에 의한 hyaluronic acid 생성의 최적 배지 조성은 batch cultre 조건에서 0.1% 쇠고기 추출물, 0.1% 효모 추출물, 3.0% 포도당 2.0% peptone, 0.1% 식염 및 0.5% $CaCO_3$이였으며, 배지의 포기 pH는 7.5로서 $37^{\circ}C$에서 36시간 진탕배양하는 것이 양호했다. 특히 공시균의 생육에 수반되어 hyaluronic acid가 생성되므로 배지의 pH를 중화하기위해 $CaCO_3$의 첨가는 필수적이었다.

• Archives of Plastic Surgery
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• 제47권4호
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• pp.297-300
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• 2020

Hyaluronidase, an enzyme that breaks down hyaluronic acid, has long been used to increase the absorption of drugs into tissue and to reduce tissue damage in cases of extravasation of a drug. With the increasing popularity of hyaluronic acid filler, hyaluronidase has become an essential drug for the correction of complications and unsatisfactory results after filler injection. For this reason, when performing procedures using hyaluronic acid filler, a sufficient knowledge of hyaluronidase is required. In order for hyaluronidase to dissolve a hyaluronic acid filler, it must interact with its binding sites within the hyaluronic acid. The reaction of a filler to hyaluronidase depends on the hyaluronic acid concentration, the number of crosslinks, and the form of the filler. Hyaluronidase is rapidly degraded and deactivated in the body. Therefore, in order to dissolve a hyaluronic acid filler, a sufficient amount of hyaluronidase must be injected close to the filler. If the filler is placed subcutaneously, injection of hyaluronidase into the filler itself may help, but if the filler is placed within a blood vessel, it is sufficient to inject hyaluronidase in the vicinity of the vessel, instead of into the filler itself. Allergic reactions are a common side effect of hyaluronidase. Most allergic reactions to hyaluronidase are local, but systemic reactions may occur in infrequent cases. Since most allergic responses to hyaluronidase are immediate hypersensitivity reactions, skin tests are recommended before use. However, some patients experience delayed allergic reactions, which skin tests may not predict.

• Journal of Dairy Science and Biotechnology
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• 제38권3호
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• pp.146-153
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• 2020

Recently, hyaluronic acid, which is the most popular among various biomaterials, has been used in osteoarthritis treatment, eye surgery, and wound healing. This study was carried out to determine the physicochemical and organoleptic properties of kefir containing various concentrations of hyaluronic acid. The pH value of hyaluronic-acid-containing kefir ranged from 3.58 to 3.46. Moreover, for hyaluronic-acid-containing kefir, the score of taste ranged from 4.75 to 4.0, the flavor ranged from 4.0 to 3.38, the color ranged from 4.38 to 4.0, the texture ranged from 4.38 to 3.75, and the overall acceptability ranged from 4.59 to 3.72. Overall, the organoleptic properties of hyaluronic-acid-containing kefir were better than those of the control. Because this is the first study involving hyaluronic acid and kefir, the results are valuable. The addition of hyaluronic acid did not significantly affect the physicochemical and organoleptic properties of kefir. Therefore, the commercial availability of various hyaluronic-acid-added dairy food products must be investigated.

• 한국식품영양학회지
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• 제9권3호
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• pp.242-246
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• 1996

High viscous hyaluronic acid complex from Klebsiella sp. L-10 NTG 50 mutant was purified by two-phase extraction system using PEG-K2HP04 and its physicochemical properties were Investigated. Viscosity of the purified hyaluronic acid complex was decreased as temperature and salts concentration were Increased and also showed low viscosity at below pH 5.0 and above pH 11.0. Hardness, cohesiveness and adhesiveness of the purified hyaluronic acid complex were 1, 20kg, 1.91 and 0.62, respectively. Water holding capacity was 6.9ml per gram of the purified hyaluronic acid complex powder.

• Tuberculosis and Respiratory Diseases
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• 제77권2호
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• pp.90-93
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• 2014

Hyaluronic acid is widely used in medical procedures, particularly in cosmetic procedures administered by physicians or nonmedical personnel. The materials used for cosmetic procedures by physicians as well as illegally by non-medical personnel can cause nonthrombotic pulmonary embolism (NTPE). We report the case of a woman with acute respiratory failure, neurologic symptoms and petechiae after an illegal procedure of hyaluronic acid dermal filler performed by an unlicensed medical practitioner 3 days before symptom onset. Although a few cases of NTPE after injection of hyaluronic acid have been reported yet, this is the first typical case showing a NTPE manifestation after the facial injection of hyaluronic acid.

• 대한족부족관절학회지
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• 제27권3호
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• pp.79-86
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• 2023

Numerous nonoperative treatments have been used to reduce pain and improve the clinical outcomes of patients with foot and ankle disorders. Among these treatments, hyaluronic acid and platelet-rich plasma (PRP) injections have been used increasingly. This review analyzes the recent literature reporting the efficacy and indications for hyaluronic acid and PRP injections. Hyaluronic acid and PRP can be used safely as adjuncts. Hyaluronic acid injections have shown efficacy and have a promising role as an adjuvant treatment method. In addition, its indication has expanded to various foot and ankle diseases over osteoarthritis. On the other hand, the efficacy of PRP injection has not yet been clarified, and the lack of standardization of procedures for the preparation and administration of PRP makes it difficult to establish definitive treatment indications.

• KSBB Journal
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• 제11권1호
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• pp.15-21
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• 1996

히아루론산 정제과정 중 불순물인 핵산과 단백질 을 제거하기 위하여 여러 가지 균체제거 실험을 수 행한 결과 여과에 의한 방법이 히아루론산 용액 중 의 단백질과 핵산이 가장 잘 제거되었다. 보다 효과 적요로 핵산과 단백질을 제거하기 위하여 활성탄과 규조토를 첨가하여 여과하였다. 여과시 히아루론산 의 회수율과 핵산 및 단백질 제거율을 살펴 본 결과 활성탄 농도 0.6%와 규조토 농도 1.0% 첨가하는 경우를 최적 여과조건으로 선정하였다. 에탄올 첨가 는 에단융과 히아루론산 용액을 동시에 첨가하는 경우에 히아루론산의 회수율과 핵산 및 단백질 제거율 이 가장 좋았다. 에탄올에 의한 히아루론산의 침전 시 최적조건을 찾기 위해 pH와 전도도를 변화 시키 는 실험을 수행하였다. pH와 전도도에 따른 핵산과 단백질의 제거 정도는 큰 차이를 보이지 않았고 에 탄올에 의한 히아루론산의 침전은 pH 7. 전도도 100mS(l.OM NaCI 정도에 해당)에서 최적이었으며, 이때 히아루론산의 회수율은 약 85% 정도이였 다. 에탄올 침전과 여과 과정에서 히아루론산 용액 중의 단백질은 거의 제거되었으나, 핵산은 완전히 제거되지 않았다. 핵산을 완전히 제거하기 위하여 Duolite A 7 수지를 통과시킨 결과 pH 7, 전도도 4 40mS 부근에서 핵산제거율이 65%로 가장 좋았다. 히아루론산 용액 내의 잔존 핵산은 hydroxy-apatite 0.2%를 처리하여 거의 제거 할수 있어 그 결과 불 순물인 핵산과 단백질이 각각 0.02%. 0.01 % 이하 인 의료용 히아루론산을 만들 수 있었다.