• Title/Summary/Keyword: Hydroponics

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Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

  • Choi Ki Young;Son Sung Ho;Lee Joo Hyun;Lee Yong-Beom;Bae Jong Hyang
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.298-301
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    • 2005
  • Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

Growth and Ginsenoside Content of One Year Old Ginseng Seedlings in Hydroponic Culture over a Range of Days after Transplanting (수경재배 시 1년생 묘삼 이식 후 경과일수에 따른 인삼의 생육 및 Ginsenoside 함량)

  • Jeong, Dae Hui;Lee, Dae Young;Jang, In Bae;Yu, Jin;Park, Kee Choon;Lee, Eung Ho;Kim, Young Jun;Park, Hong Woo
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.6
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    • pp.464-470
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    • 2018
  • Background: Ginseng produced by hydroponics can be cultivated without using agricultural chemicals; thus, it can be used as a raw materials for functional foods, medicines, and cosmetics. This study aimed to determine the optimal harvesting time to obtain the highest levels of ginsenoside and ginseng, as this was not previously unknown. Methods and Results: One-year-old organic ginseng seedlings were transplanted and cultivated using hydroponics for 150 days in a venlo-type greenhouse, using ginseng nursery bed soil and a nutrient solution ($NO_3{^-}-N$; 6.165, P; 3.525, K; 5.625, Ca; 4.365, Mg; 5.085, S; $5.31mEq/{\ell}$). Ginsenoside content and fresh and dry weights were higher at 120 days after transplanting than at 30, 60, 90, and 150 days. Total ginsenoside content was 11.86 times higher in the leaf and stem than in the root at 120 days after transplanting. Ginsenosides F1, F2, F3, and F5 were detected in ginseng leaves and stems. These chemical compounds are known to be effective in altering skin properties, including whitening, anti-inflammation, and anti-aging. Conclusions: Optimal harvesting time for ginseng cultivated using hydroponics was 120 days after transplanting when the biomass and ginsenoside content were highest.

Comparative Study on Growth and Yield of Far Eastern Catfish Silurus asotus and Leafy Vegetables Grown in Hybrid BFT-Aquaponics, Semi-RAS and Hydroponics (메기(Silurus asotus) 및 엽채류의 성장과 생산을 위한 Hybrid BFT 아쿠아포닉스(HBFT-AP), 반순환 양식(Semi-RAS) 및 수경재배(Hydroponics)의 비교 연구)

  • Lee, Dong-Hoon;Kim, Jin-Young;Lim, Seong-Ryul;Kim, Dal-Young;Kim, Kwang-Bae;Kim, Joo-Min;Kim, Jeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.5
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    • pp.482-495
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    • 2019
  • This study compared the productivity of fish and vegetables grown using the hybrid biofloc technology-aquaponics (HBFT-AP), a semi-recirculating aquaculture system (SRAS), and hydroponics (HP). For the study of fish productivity (HBFT-AP vs. SRAS), fish were provided feed containing 3.0% monobasic potassium phosphate (MKP) for 18 weeks. After the 18-week feeding trial, the average weight of the sampled population (n=100) was not significantly different (P>0.05), while hematocrit (PCV, %), hemoglobin (Hb, g/dL), and plasma K (mEq/L) were significantly different (P<0.05) between the two groups (HBFT-AP: 47.83%, 15.48 g/dL, and 1.39 mEq/L; SRAS 34.83%, 11.81 g/dL, and 2.48 mEq/L). Leaf vegetable productivity (HBFT-AP vs. HP) was compared in three experiments (EXP 1-3), and slower growth was observed in both groups in EXP 2, in which pH was maintained at 5.0 or less throughout the experiment. During the 18-week feeding trial, total ammonia nitrogen (TAN), $NO_3-N$, and $PO_4-P$ levels increased with time in the HBFT-AP system, while the concentration of $NO_2-N$ remained below 0.1 mg/L throughout the study.

Effect of Humus and Micronutrient Element Content on Nutrient Absorb and Growth of Lettuce in Hydroponics (Humus 첨가와 미량원소 수준이 상추의 무기성분 흡수 및 생육에 미치는 영향)

  • 이성재;서명훈;이상우;심상연;이수연
    • Journal of Bio-Environment Control
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    • v.8 no.3
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    • pp.183-191
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    • 1999
  • In the recirculating hydroponics, pH of nutrient solution was gradually decreased until the middle of growing-period, and then began to go up and reached the initial level at the latter of growing-period regardless of Cu·Zn content. Changes of pH was gently down for supply of humus in nutrient solution. The growth of lettuce was good with supply of humus, but it was decreased as Cu·Zn content was increased. Although leaf chlorosis was caused by excessive content of Cu·Zn as 5, 10 ppm, it was controlled by adding humus in nutrient solution, and fresh weight of lettuce was obviously improved by adding humus.

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Uptake and Phytotoxicity of TNT in Onion Plant

  • Kim, Jaisoo;Yavuz Corapcioglu;Malcolm C. Drew
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.102-106
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    • 2003
  • The uptake of $^{14}C$-2, 4, 6-trinitrotoluene (TNT) in hydroponics was studied using onion plants. Of the total TNT mass (5 $\mu\textrm{M}$ concentration), 75% was in the roots, 4.4% in the leaves, and 21% in the external solution at 2 days, The percent distribution in roots was lower with higher concentration in the external solution, but in leaves it was comparable at all concentrations (5-500 $\mu\textrm{M}$). Root concentration factor (RCF) in hydroponics was more than 85 in constant hydroponic experiment (CHE) at 5 $\mu\textrm{M}$ and 150 in non-constant hydroponic experiment (NHE) at 5 $\mu\textrm{M}$. The maximum RCF values in the hydroponic system were greater with lower solution concentration. Transpiration stream concentration factor (TSCF) values in the present study (NHE only: 0.31-0.56) were relatively similar to the values with predicted values (0.43-0.78), increasing with higher external TNT concentration. For phytotoxicity tested in hydroponics and wet paper method, 500 $\mu\textrm{M}$ was toxic to onion plant, 50 $\mu\textrm{M}$ was non-toxic for plant growth but limited the transpiration rate, and 5 $\mu\textrm{M}$ was non-toxic as control.

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Study on Automatic Mixing of Nutrient Solution Using ion Electrodes for Closed-Loop Hydroponics (이온전극을 이용한 순환식 양액 자동 조제에 관한 연구)

  • 김민규;류관희;장유섭;김기영
    • Journal of Biosystems Engineering
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    • v.27 no.5
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    • pp.425-432
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    • 2002
  • This study was carried out to develop an automatic mixing system of nutrient solution for closed-loop hydroponics using ion electrodes. The results of the study are summarized as follows: 1. It appeared that ion-electrodes had not to be soaked into nutrient solution for a long time since it was much less durable than EC or PH sensors. Once ion-electrodes were soaked into real nutrient solution for a long time, they became unstable. 2. ion measurement modules, which were able to sample recirculated nutrient solution and easily wash and dry ion-electrodes, were developed in order to use ion-electrodes continuously. 3. The results of calibration tests on three kinds of ion electrodes presented that the time required to read measurement data was over 30 seconds. Using the calibration data the regression equations for the ion electrodes were developed. 4. An automatic nutrient-solution mixing system using the three kinds of ion electrodes was developed and then its accuracy was examined. The control errors of the mixing system using ion electrodes were in the range of 9.8 to 12%.

Changes in the Nutrient Components Associated with the Growth of Lettuce in Circulating Hydroponics (순환식 양액재배에서 상추의 성장에 따른 양액성분의 변화)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.24 no.8
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    • pp.1075-1084
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    • 2015
  • This study was conducted to investigated the changes in the nutrient components ($NO_3{^-}-N$, $NH_4{^+}-N$, $PO_4{^{3-}}P$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$) and environmental parameters (electrical conductivity, total dissolved solids and pH) on the leaf lettuce (Lactuca sativa L.) grown with hydroponics. Recirculating hydroponic cultivation system was consisted of planting port, LED lamp, water tank, and circulating pump for hydroponic. Nutrient solution was used in the standard solution for Japan vegetables experimental station and commercial hydroponic. The result showed that electrical conductivity (EC), total dissolved solids (TDS) and pH, depending on the growth of lettuce decreased continuously. With the growth of the lettuce, nitrate nitrogen, ammonia nitrogen, phosphate phosphorus were required for periodic replacement. The number of pH compensation due to the growth of lettuce are the most high. The concentration of $Ca^{2+}$ and $Mg^{2+}$ during the lettuce growth showed no significant change. However, $K^+$ concentration increased due to the replacement with nitrogen and phosphorus. Electric conductivity and total dissolved solids with total nutrient concentration showed the linear relationship and the correlation coefficient $R^2$ were 0.8601 and the 0.827, respectively.

The Arduino based Window farm Monitoring System (아두이노를 활용한 창문형 수경재배 모니터링 시스템)

  • Park, Young-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.5
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    • pp.563-569
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    • 2018
  • This paper is on the implementation of a system for automatically monitoring window farm hydroponics based on Arduino (utilizing Arduino's open source code) emerging as the icon of the Fourth Industrial Revolution. A window farm, which means window-type hydroponics, is offered as an alternative to fulfill the desires of people who want to grow plants aside from the busy daily life in the city. The system proposed in this paper was developed to automatically monitor a window farm hydroponics cultivation environment using the Arduino UNO board, a four-charmel motor shield, temperature and humidity sensors, illumination sensors, and a real-time clock module. Modules for hydroponics have been developed in various forms, but power consumption is high because most of them use general power and motors. Since it is not a system that is monitored automatically, there is a disadvantage in that an administrator always has to manage its operational state. The system is equipped with a water supply that is most suitable for a plant growth environment by utilizing temperature, humidity, and light sensors, which function as Internet of Things sensors. In addition, the real-time clock module can be used to provide a more appropriate water supply. The system was implemented with sketch code in a Linux environment using Raspberry Pi 3 and Arduino UNO.

Chemical Characteristics of Ground Water for Hydroponics and Waste Nutrient Solution after Hydroponics in Chungbuk Area (충북지역 양액 재배용 지하수 및 폐양액의 화학적 특징)

  • Lee, Gyeong-Ja;Kang, Bo-Goo;Lee, Ki-Yeol;Yun, Tae;Park, Seong-Gyu;Lee, Cheol-Hee
    • Korean Journal of Environmental Agriculture
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    • v.26 no.1
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    • pp.42-48
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    • 2007
  • This survey has been conducted to obtain basic data of the quality of ground water for hydroponics and waste nutrient solution after hydroponics in hydroponic farms in Chungbuk area. Ground water samples were collected and analyzed at 19 sites of hydroponic farms. Waste nutrient solution samples were analyzed at 15 sites selected of them. The values of several components in ground water for hydroponics were as follows. pH range was shown from 6.2 to 7.7 and the average was 6.8. EC range was shown from 0.10 to 0.45 dS $m^{-1}$ and the average 0.23 dS $m^{-1}$. $NO_3-N$ concentrations was ranged from 0.12 to 13.77 mg $L^{-1}$, $SO_4^{2-}$ concentrations was ranged from 1.84 to 63.01 mg $L^{-1}$ and $Cl^-$ concentrations were ranged from 10.46 to 72.09 mg $L^{-1}$. Average values of $NO_3-N$, $SO_4^{2-}$ and $Cl^-$ were 4.00 mg $L^{-1}$, 12.70 mg $L^{-1}$ and 27.57 mg $L^{-1}$, respectively. $Ca^{2+}$, $Mg^{2+}$ and $Na^+$ concentrations were ranged from 3.24 to 36.99 mg $L^{-1}$, 1.44 to 14.93 mg $L^{-1}$ and 6.12 to 25.25 mg $L^{-1}$, respectively. Average concentrations were 13.06 mg $L^{-1}$ in $Ca^{2+}$, 6.02 mg $L^{-1}$ $Mg^{2+}$ and 12.08 mg $L^{-1}$ in $Na^+$. In waste nutrient solution after hydroponics, pH range was shown from 4.3 to 8.8 and the average was 6.7. EC range was shown from 0.44 to 2.37 dS $m^{-1}$ and the average 1.15 dS $m^{-1}$. Range of $NO_3-N$, $PO_4-P$, $K^+$, $Ca^{2+}$, $Mg^{2+}$ and $Na^+$ in waste nutrient solution were $10{\sim}212$, $0.56{\sim}26.1$, $10{\sim}295$, $16{\sim}215$, $9{\sim}54$ and $10{\sim}53$ mg $L^{-1}$ respectively. Average concentration were 100 mg $L^{-1}$ in $NO_3-N$, 12.15 mg $L^{-1}$ in $PO_4-P$, 99 mg $L^{-1}$ in $K^+$, 78 mg $L^{-1}$ in $Ca^{2+}$, 26 mg $L^{-1}$ in $Mg^{2+}$ and 26 mg $L^{-1}$ in $Na^+$. Inorganic matters in waste nutrient solution after hydroponics was higher than that of ground water for hydroponics.

Improving Work Adjustment Skills in Students with Mental Retardation Using Hydroponics Program (수경재배 프로그램을 통한 지적 장애학생의 직업적응력 증진)

  • Joo, Byung-Sik;Park, Sin-Ae;Son, Ki-Cheol
    • Horticultural Science & Technology
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    • v.30 no.5
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    • pp.586-595
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    • 2012
  • This study was conducted to determine the effects of horticultural therapy (HT) program using hydroponics on work adjustment skills of students with mental retardation. Based on the critical role transitional model and special education curriculum for agriculture, especially hydroponics, HT program (total 22 sessions) using hydroponics procedure for Lettuce (Lactuca sativa L. 'Asia Heuk Romaine') was developed. Fourteen (10 males, 4 females) graded $1^{st}$ to $2^{nd}$ with intellectual disabilities were recruited from a special education class in a high school located in Inchon, Korea and then a special farm for hydroponics in Inchon, Korea was offered for the HT program. The students with intellectual disabilities participated in the HT program for 4-month (from September to December of 2011, twice a week, approximately 60 minutes per session). Before and after the HT program, the McCarron assessment neuromuscular development, emotional behavioral checklist, interpersonal negotiation strategies, and KEPAD picture vocational interest test were performed by the teachers and horticultural therapists. As the results, the students significantly improved motor performance (p = 0.002), emotional behavioral strategies (p = 0.00), and interpersonal negotiation strategies (p = 0.05). However, no significant difference between before and after the HT program for vocational interest was observed. In conclusion, the HT program using hydroponics, consists of simple and easy tasks so that it would be applicable for the students with intellectual disabilities positively affected to work adjustment skills by improving the motor performance, emotional behavioral strategies, and interpersonal negotiation strategies. Additionally, HT programs using hydroponics with various kinds of vegetables are required to develop and to apply in practical settings for improving work adjustment skills.