• Title, Summary, Keyword: Immunoglobulin

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Structural Characterization of an IgM-like Immunoglobulin in the Serum of Swamp Eel, Monopterus albus (드렁허리(Monopterus albus) 혈청내 lgM유사 면역글로불린의 구조적 특성)

  • 손영종;이정우;장정순
    • The Korean Journal of Zoology
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    • v.33 no.1
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    • pp.103-107
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    • 1990
  • lgM-like immunoglobulin was purified from the immune serum of M albus which immunized with bovine Serum albumin(BSA) as an antigen(Ag) and characterized. The Ag-specific antibody activity of the immune serum was increased after the immunization. The purified lgM-like immunoglobulin had a tetrameric structure which had a molecular weight of 800 kD and the monomer of IgM-like Ig had a mass of 199 kD which was composed of two heavy chains (Mol. wt. 70 kD) and iwo light chains (Mol. wt. 29.5 kD). The IgM-like Ig showed hemaggluti nating activity to mammalian RBC slightly.

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Evaluation on Tongue Coating, Volatile Sulfur Compounds, Salivary Flow Rate and Secretory Immunoglobulin A in Saliva in Patients with Oral Malodor (구취환자의 설태, 휘발성 황화합물, 타액분비율 및 타액내 secretory Immunoglobulin A에 대한 평가)

  • Kim, Jin-Sung;Eom, Guk-Hyeon
    • The Journal of Internal Korean Medicine
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    • v.29 no.1
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    • pp.258-264
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    • 2008
  • Objectives : The aim of this study was to confirm the characteristics of oral malodor patients by evaluating the differences of salivary flow rate, secretory immunoglobulin A (sIgA) level in saliva between the patient and control groups, and the correlation with the tongue coating, volatile sulfur compound (VSC), salivary flow rate and sIgA level in saliva in the patients group. Methods : Forty-seven patients with oral malodor and twenty healthy volunteers were included in this study. Their tongue coating was assessed with the Winkel tongue coating index, and salivary flow rate, sIgA concentrations in saliva and the level of VSC in oral cavity were measured. Results : There were no significant differences of the salivary flow rate and the sIgA level in saliva between the patient and control groups, but there was a significant relationship between the accumulation of tongue coating and the level of VSC in oral cavity. Conclusions : Our results suggest that tongue coating is closely related to oral malodor, but further studies are needed to confirm the relationship between tongue coating and sIgA level in saliva.

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Bickerstaff's Brainstem Encephalitis Treated with Immunoglobulin (면역글로불린으로 치료한 Bickerstaff 뇌간뇌염 1예)

  • Kim, Beom Joon;Choi, Yoon Jae;Kim, Nam-Hee;Hong, Yoon-Ho;Sung, Jung-Joon;Han, Moon-Ku;Park, Seong-Ho;Lee, Kwang-Woo;Park, Kyung Seok
    • Annals of Clinical Neurophysiology
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    • v.8 no.1
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    • pp.94-97
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    • 2006
  • Bickerstaff's brainstem encephalitis (BBE) is a rare disorder with acute ophthalmoparesis, ataxia, consciousness disturbance, and pyramidal signs of suspected autoimmune origin. A-62-year-old woman developed acute diplopia, dysarthria, gaze-paretic nystagmus and gait ataxia. Her mental status fell subsequently into stupor. Brain MRI and nerve conduction study showed no significantly abnormal findings. Electroencephalography revealed diffuse low voltage slowings. After treating with intravenous immunoglobulin, she demonstrated rapid clinical recovery. This case suggests that immunoglobulin can be an alternative option in BBE treatment, especially when plasmapheresis and corticosteroids are difficult or contraindicated.

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CLA Treatment Effect on Immunsuppressive Effect of Corticosteroid in Mouse (Mouse에서 CLA의 투여가 면역성 증진에 미치는 영향)

  • 박종국;김진영;이병한;임좌진;정병현
    • Journal of Veterinary Clinics
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    • v.20 no.1
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    • pp.79-85
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    • 2003
  • Dietary conjugated linoleic acid(CLA) has been shown to affect immune function. Thus, the objective of this study was to investigate the effects of CLA on the mice that treated prednisone. Mice were randomized into 6 groups and fed diet containing either 0(control, P), 0.5%(CLA1, CP1) or 1.5%(CLA2, CP2) CLA for Sweets. Before 1 week of finishing diet supplement CP1, CP2, and P group treated the prednisone by subcutaneous injection. The levels of serum immunoglobulin A, G, E, gut lumen s-IgA, MLN immunoglobulin A, body weight, mucosal protein was compared. The level of serum IgA in CLA1, CLA2, CP1, and CP2 group increased, while which of P group was decreased. The level of serum IgG in CLA 1 group increased, while which of the other group no differences. Serum IgE level showed no difference and the immunoglobulin production in MLN lymphocyte in CLA 1 group increased. The level of gut lumen s-IgA in P group showed decreased, while which of the other group showed no differences. These results support the view that CLA supplement partially enhance the cell-mediated immunity and overcome the immunosuppressive effect of prednisone.

Production of Monoclonal Antibodies Against the Immunoglobulin M of Olive Flounder Paralichthys Olivaceus (넙치(Paralichthys olivaceus)의 immunoglobulin M에 대한 단클론 항체 생산)

  • Kim, Wi-Sik;Kim, Ki-Hong;Kim, Choon-sup;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.2
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    • pp.169-174
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    • 2017
  • Immunoglobulin M (IgM) was purified from olive flounder Paralichthys olivaceus sera using mannan-binding protein (MBP) and protein L affinity columns (designated as MBPIgM and ProLIgM, respectively). A monoclonal antibody (MAb) against olive flounder IgM was produced. The MBPIgM and ProLIgM had apparent molecular weights of 77, 73, and 28 kDa in SDS-PAGE. Nine hybridomas secreting MAbs against olive flounder IgM were established: five MAbs for MBPIgM (1, 2, 3, 4, and 5) and four for ProLIgM (6, 7, 8, and 9). Western blotting indicated that seven MAbs recognized heavy (H; MAbs 1, 2, 3, 4, 5, 6, and 7) chains and one recognized light (L; MAb 9) chains of IgM, while MAb 8 did not recognize IgM. The results of enzyme-linked immunosorbent assay (ELISA) with bovine serum albumin (BSA, antigen) and the nine MAbs revealed that the optical density (OD) values of sera differed significantly between BSA- and non-immunized fish, despite some sera from non-immunized fish with slight high OD values. These results suggest that the MAbs produced in this study reacted specifically with the IgM from olive flounder.

Age related changes of ICAM-1 expression in the heart of mice (마우스 심장에서 노화에 따른 ICAM-1 표현양상의 변화)

  • 류시윤;정규식;조성환;윤원기;박배근;김무강;권오덕;김성호
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.30-36
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    • 1997
  • It is well estabilished that IL-4, IL-6, $IFN{\gamma}$ and immunoglobulin production are increased as a consequence of advancing age, and endothelial cells increase their expression of pro-inflammatory surface protein, such as ICAM-1, as a consequence of contact with inflammatory cytokines, including IL-1, $TNF{\alpha} or INF{\gamma}.$ This study was designed to define the relationship between age-associated changes of cytokines and immunoglobulin production and ICAM-1 expression in aged mice. Serum from aged mice had elevated IL-6 and immunoglobulin levels compared to mature adult controls and activation-induced production of $IFN{\gamma}$ of splenocytes from aged mice were increased above normal adult level. By immunoperoxidase staining, ICAM-1 in hearts of normal adult mice was expressed occasionally at low levels, but in aged mice, the number of positive blood vessels for ICAM-1 were increased and also the immunoreactivity were stronger. Therefore, these finding indicate that the altered expression of ICAM-1 eith normal aging may actually be correlative to these age-related changes in cytokiness and immunoglobulin production.

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Relationship between vitamin D levels and intravenous immunoglobulin resistance in Kawasaki disease

  • Jun, Jae Sung;Jung, Young Kwon;Lee, Dong Won
    • Clinical and Experimental Pediatrics
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    • v.60 no.7
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    • pp.216-220
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    • 2017
  • Purpose: Vitamin D is associated with various pathological conditions such as cardiovascular diseases and cancer. We investigated the relationship between vitamin D and Kawasaki disease (KD). Methods: We performed a retrospective review of the medical records of patients with KD between February 2013 and March 2016 in Daegu Fatima Hospital. Study participants were grouped according to vitamin D serum concentration. Group 1 included patients with 25(OH)-vitamin D ${\geq}20ng/mL$. Group 2 included patients with 25(OH)-vitamin D <20 ng/mL. We analyzed the clinical characteristics and laboratory data of the 2 groups. Results: Of the 91 patients, 52 were included in group 1, and 39 in group 2. Group 1 patients had significantly higher levels of calcium, phosphate, albumin and sodium than group 2 patients did. There were no differences in clinical characteristics, but the proportion of patients with polymorphic rash was significantly higher in group 2. Resistance to intravenous immunoglobulin was more frequent in group 2 (P=0.023). No significant difference in the incidence of coronary artery complications was observed. Conclusion: Low vitamin D levels are associated with resistance to intravenous immunoglobulin therapy in KD. Vitamin D deficiency might be a risk factor for immunoglobulin resistance in KD.

The Effects of the Low Power Wavelengths of Helium-Neon Infra- Red Laser on the Changes of the Serum Immunoglobulin Concentration in Burn Rats (저 에너지 레이저 파장이 화상쥐 혈청면역물질 농도 변화에 미치는 영향)

  • Rho Min-Hee;Yong Joon-Hwan
    • The Journal of Korean Physical Therapy
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    • v.12 no.3
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    • pp.287-292
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    • 2000
  • This studies were to investigate the effects of low power wavelengths Helium-Neon Infra-Red(He-Ne 1R) laser on the changes of the serum immunoblobulin(Ig) concentrations in bum rats, The thirty Spraque-Dawley adult male rats were assigned to the 5 groups: the experimental groups(3). the bum control group(1) and the control group(1). There was made three degree burn by the 290 mW IR on the back oreach rats, from 3 days after being burned. the experimental laser groups were irradiated low power wavelengths(292Hz- 1168Hz, 4672Hz) He-Ne IR laser far 5 minutes every day during the 7days. The results were as follows; 1. The concentrations of immunoglobulin G(Ig G) in serum of all experimental groups on the treated with the low power wavelengths of the He-Ne IR laser during the 5 minutes far 7 days were significantly lower than those of bum groups, but those 1168 Hz group were significantly higher than those of the 292 Hz and 4672 Hz groups. 2. There were significantly decreased the change of the level of immunoglobulin M(Ig M) in serum of all experimental groups on the treated with 5 minutes laser fer 7 days to the burn control group. and were significantly increased on treated with more higher wavelengths groups to those of lower groups. 3. The concentrations immunoglobulin E(Ig E) in serum of the 292Hz wavelengths groups were significantly lower than those in bum control group. As above results. the changes of the immunoglobulin in serum levels on the healing process have meaningful effected with the low power wavelengths role on the treated with He-Ne IR laser.

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The Effects of the low power intensity He-Ne IR laser on the change of the Serum Immunoglobulin components in burn rat (저 에너지 레이저 강도가 화상흰쥐의 혈청 면역글로불린의 성분 변화에 미치는 효과)

  • Rho Min-Hee;Kim Jae-Young
    • The Journal of Korean Physical Therapy
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    • v.12 no.3
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    • pp.469-474
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    • 2000
  • This studies were to investigate the effects of low power intensity Helium-Neon Infra-Red(He-Ne IR) laser on the changes of the serum immunoglobulin(1g) components in burn rats, The fourty eight Spraque-Dawley adult male rats were assigned to the 8 groups: the experimental groups(4), the burn control groups(2) and the control groups(2) There was made three degree burn by the 250mW IR on the back of each rats, from 3 days after being burned, the experimental laser groups were irradiated low power He-Ne IR laser for 3 minutes and 4.5 minutes every day during the 7days and 21 days. The results were as follows: The concentrations of immunoglobulin G(Ig G) in serum of burn rats on the treated with during the 3minutes laser for 7 days were higher than those of burn and 4.5 minutes laser groups. There were significantly decreased the change of the level of immunoglobulin M(Ig M) in serum of burn rats on the treated with 3 minutes laser for 7 days to the control group, but were significantly increased on the 3minutes and 4.5 minutes laser groups for 21 days to the those of burn and control groups, and the levels of the Ig M on the 3minutes laser group for 21 days were higher than those of 7 days. The concentrations of immunoglobulin E(Ig E) in serun of the burn rats on the experimental and burn groups were significantly lower than those in control group. As above results, The changes of the level of immonoglobulin components in serum have meaningful role in the healing process on the treated with the low pewer intensity of HE-Ne IR laser.

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Effects of Postpartum Massage Program on Stress response in the Cesarean section Mothers (산후마사지프로그램이 제왕절개술 산모의 스트레스 반응에 미치는 효과)

  • 이성희
    • Journal of Korean Academy of Nursing
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    • v.30 no.2
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    • pp.488-497
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    • 2000
  • The purpose of this study was to explore the effect of a postpartum massage program on stress response in the Cesarean section mothers. The study focused on evaluating the effect of postpartum massage program on mood, anxiety, skin temperature and concentration of saliva and breast milk immunoglobulin A in the Cesarean section mothers. This study was designed as a nonequivalent control group pretest-posttest quasi-experimental study. Twenty-eight Cesarean section mothers were selected as experimental group, whereas twenty- seven were control group. The postpartum massage program consisted of 20 minutes of warm-up, massage and ending phases and used once a day. During each program, there were 4 minutes of warm-up, 14 minutes of massage on back, axillary and breasts, and 2 minutes of ending. Massage were used for the experimental group by the same investigator 20 times per minute. The massage technique used were efflurage, petrissage, accupressure, kneading and vibration. Skin temperature was monitored with YSI Tele-thermometer(Simpson electric Co., USA) before and after massage program. The concentration of immunoglobulin A in saliva and breast milk was analyzed by immunoturbididimeter assay(Cobas INTEGRA, Swiss) before and after massage program. Also at this time mood and anxiety were measured by self-report. The data were analyzed using SPSS version 7.5 and hypothesis was tested with ANCOVA analysis and Pearson coefficient correlation. The results were as follows : 1) Score of mood increased significantly after use of postpartum massage program. 2) Level of anxiety decreased significantly after use of postpartum massage program. 3) Skin temperature increased significantly after use of postpartum massage program. 4) Concentration of saliva immunoglobulin A increased significantly after use of postpartum massage program. 5) Concentration of breast milk immunoglobulin A did not change significantly after use of postpartum massage program. 6) After use of postpartum massage program, there was significant correlation between psychological stress response and physiological stress response.The results suggest that postpartum massage program can be effective nursing intervention to reduce stress response in the postpartum mothers under stress.

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