• Title/Summary/Keyword: Immunoglobulin

Search Result 1,137, Processing Time 0.029 seconds

Effect of Subclinical Mastitis on Milk of Cross Bred Sahiwal × Jersey Cows: A Biochemical Study

  • Kalorey, D.R.;Kurkure, N.V.;Nigot, N.K.;Patil, M.P.;Pathak, V.P.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.14 no.3
    • /
    • pp.382-383
    • /
    • 2001
  • The experiment was undertaken to study the effect of subclinical mastitis on the biochemical parameters of milk in crossbred (Sahiwal $\times$ Jersey) cows. Subclincial mastitis was judged using California Mastitis Test and graded on a scale of 0 to 2. Twenty six milk samples of each grade were subjected to somatic cell count. Separated milk whey was analysed for total protein, immunoglobulin and trace mineral content. Results indicate linear increases in somatic cell count, total protein and immunoglobulin concentrations in milk with increase in grade of subclinical mastitis. However, copper, zinc, manganese and iron concentration in milk was not affected by grade of mastitis.

Effect of Heparin on the High Affinity KGF and aFGF Binding to the Chimeric KGFR-HFc

  • Cheon, Hyae-Gyeong
    • BMB Reports
    • /
    • v.29 no.3
    • /
    • pp.205-209
    • /
    • 1996
  • To investigate the role of heparin in keratinocyte growth factor (KGF) and acidic fibroblast growth factor (aFGF) high affinity binding to the KGF receptor (KGFR), a cell free system was established which utilized a secreted chimeric molecule between the KGFR extracellular domain and the immunoglobulin heavy chain Fc domain (KGFR-HFc). KGFR-HFc was purified from NIH 3T3 cells and demonstrated the binding of $[^3H]-heparin$ as well as heparin Sepharose. Scatchard analysis showed that the dissociation constant for heparin binding to KGFR-HFc was 140 nM. High affinity KGF and aFGF binding to KGFR-HFc remained unchanged after treatment with 0.6 M NaCl, which is the concentration sufficient to release any bound heparin to the KGFR-HFc. These results strongly suggest that although the KGFR interacts with heparin, the presence of heparin is not absolutely required for high affinity binding of either KGF or aFGF to the KGFR.

  • PDF

Helicobacter pylori 특이 계란항체의 생산 및 특성

  • Kim, Byoun-Jae;Kang, Byung-Hwa;Kim, Tae-Yong;Kim, Tae-Han;Kim, Kee-Won
    • Microbiology and Biotechnology Letters
    • /
    • v.25 no.6
    • /
    • pp.612-616
    • /
    • 1997
  • IgY (egg yolk immunoglobulin) against Helicobacter pylori was produced by immunizing hen with some Helicobacter pylori antigens. H. pylori whole cell, whole cell lysates, partially purified urease and p54 protein, which showed high antigenicity in mice, were used as immunogens. Four hens were immunized with these immunogens three times. IgY was purified from immunized egg yolk with polyethylene glycol (M.W. 8000) and its anti-H. pylori titer was determined by enzyme linked immunosorbent assay (ELISA). The anti-H. pylori titer reached peak at 8 weeks and was maintained over 20 weeks. H. pylori cells were agglutinated with these purified IgY and the specificity of these purified IgY was detected with immunoblotting.

  • PDF

Regulation of Immune Responses by the Activating and Inhibitory Myeloid-Associate Immunoglobuline-Like Receptors (MAIR) (CD300)

  • Shibuya, Akira;Nakahashi-Oda, Chigusa;Tahara-Hanaoka, Satoko
    • IMMUNE NETWORK
    • /
    • v.9 no.2
    • /
    • pp.41-45
    • /
    • 2009
  • Activating and inhibitory cell surface receptors play important roles in regulation of immune responses. Recent progress has demonstrated that many inhibitory receptors pair with activating, as well as inhibitory, isoforms, both of whose genes are located in small clusters on a chromosome. We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300). MAIR is a multigene family consisting of nine genes on a small segment of mouse chromosome 11. MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells. Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

A Biotin-avidin Labeled Enzyme Immunoassay for the Quantitation of Serum TSH Using Protein-layered Solid Phase

  • Choi, Myung-Ja;Song, Eun-Young;Chung, Tai-Wha
    • Archives of Pharmacal Research
    • /
    • v.21 no.3
    • /
    • pp.231-235
    • /
    • 1998
  • A sensitive enzyme immunoassay for serum TSH has been developed utilizing the tight binding between biotin and avidin, and three layered protein polystyrene beads as solid phase. To increase binding capacity of TSH and sensitivity of the assay, the polystyrene beads were coated sequentially with mouse immunoglobulin as first layer, rabbit antimouse immunoglobulin as second layer and monoclonal anti-TSH as third layer. A serum sample was incubated simultaneously with a monoclonal anti-TSH immobilized polystyrene beads and a second monoclonal anti-TSH covalently attached to biotin. After washing, the antibody bound serum TSH-anti-TSH-biotin complex is reacted with horseradish peroxidase (HRP)-labeled avidin. Following second wash, the bound HRP activity was measured calorimetrically. Reproducible results were obtained within 4 hours for serum TSH in the range between $0{\mu}\textrm{IU}$ml and ${50}{\mu}\textrm{IU}$ml with detection limit of $0.1{\mu}\textrm{IU}$ per test.

  • PDF

Development of diagnostic methods for rotavirus from pigs using monoclonal antibody (단크론 항체를 이용한 돼지 로타바이러스의 진단법 개발)

  • Kang, Shien-young
    • Korean Journal of Veterinary Research
    • /
    • v.32 no.4
    • /
    • pp.569-577
    • /
    • 1992
  • Group A 로타바이러스 VP6에 특이적으로 반응하는 단크론 항체를 이용하여 로타바이러스 감염이 의심되는 돼지 분변으로부터 로타 바이러스를 검색할 수 있는 효소면역측정법을 개발하였다. 이 효소면역측정법에서는 capture antibody로서 protein A-sepharose를 이용하여 단크론 항체로부터 순수 분리한 immunoglobulin을 사용하였으며 detecting antibody는 토끼 면역혈청으로부터 순수 분리한 immunoglobulin에 biotin을 label하여 사용하였다. 개발된 효소면역측정법의 민감도와 특이성을 전자현미경법 및 형광항체법의 것과 비교하여 보았을 때 서로 유사하였으며 분변재료로부터 로타바이러스를 검색하는데 유용한 것으로 나타났다. 개발된 효소면역측정법은 야외로부터 로타바이러스 검색을 위하여 수집된 많은 양의 분변재료를 실험실내에서 screen하는데 유용하게 사용될 것으로 생각된다.

  • PDF