• Title/Summary/Keyword: IncP

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A Series of IncQ-Based Reporter Plasmids for Use in a Range of Gram-Negative Genera

  • O'Sullivan, Laura E.;Nickerson, Cheryl A.;Wilson, James W.
    • Journal of Microbiology and Biotechnology
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    • v.20 no.5
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    • pp.871-874
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    • 2010
  • Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.

Low cost, printed P-OLED displays for entry into the flexible display market

  • MacKenzie, J.Devin;Breeden, J.J.;Carter, S.A.;Chen, J.P.;Hinkle, P.;Jones, E.;Kreger, M.A.;Nakazawa, Y.;Roeloffs, R.;Vo, Vung;Wilkinson, M.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.641-643
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    • 2006
  • Add-Vision has developed a low-cost print technology for P-OLED displays on flexible substrates that meets several essentials for a new technology including: (1) Functionality including low DC voltage and wide color gamut; (2) Utilization of inexpensive tools; (3) Performance matching entry applications and markets. AVI's process is based on large-area printing of a combination of doped emissive and air-stable cathode inks utilizing truly low-cost tools to create printed P-OLEDs.

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Construction of Conjugative Gene Transfer System Between E. coli and Moderately Thermophilic, Extremely Acidophilic Acidithiobacillus caldus MTH-04

  • Liu, Xianggmei;Lin, Jianqun;Zhang, Zheng;Bian, Jiang;Zhao, Qing;Liu, Ying;Lin, Jianqiang;Yan, Wangming
    • Journal of Microbiology and Biotechnology
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    • v.17 no.1
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    • pp.162-167
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    • 2007
  • A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-hostrange IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-hostrange IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10. The $Km^r\;and\;Sm^r$ selectable markers from these plasmids were successfully expressed in A. caldus MTH-04. Futhermore, the IncP and IncQ plasmids were transferred back into E. coli cells from A. caldus MTH-04, thereby confirming the initial transfer of these plasmids from E. coli to A. caldus MTH-04. All the IncP and IncQ plasmids studied were stable in A. caldus MTH-04. Consequently, this development of a conjugational system for A. caldus MTH-04 will greatly facilitate its genetic study.

Leadframe SiP with Conformal Shield

  • Kim, ByongJin;Sim, KiDong;Hong, SeoungJoon;Moon, DaeHo;Son, YongHo;Kang, DaeByoung;Khim, JinYoung;Yoon, JuHoon
    • Journal of the Microelectronics and Packaging Society
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    • v.23 no.4
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    • pp.31-34
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    • 2016
  • System In Package (SiP) is getting popular and momentum for the recent wearable, IoT and connectivity application apart from mobile phone. This is driven by market demands of cost competitive, lighter and smaller/thinner and higher performance. As one of many semiconducting assembly products, Leadframe product has been widely used for low cost solution, light/ small and thin form factor. But It has not been applied for SiP although Leadframe product has many advantages in cost, size and reliability performance. SiP is mostly based on laminate substrate and technically difficult on Leadframe substrate because of a limitation in SMT performance. In this paper, Leadframe based SiP product has been evaluated about key technical challenges in SMT performance and electrical shield technology. Mostly Leadframe is considered not available to apply EMI shield because of tie-bar around package edge. In order to overcome two major challenges, connection bars were deployed properly for SMT pad to pad and additional back-side etching was implemented after molding process to achieve electrical isolation from outer shield coating. This product was confirmed assembly workability as well as reliability.

The Prevalence Study of TMD and the Associated Factors in Korean Malocclusion Patients (한국인 부정교합자의 측두하악장애(TMD) 유병율과 그 기여요인에 관한 연구)

  • Kim, Myung-Hee;Nahm, Dong-Seok
    • The korean journal of orthodontics
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    • v.27 no.4 s.63
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    • pp.523-538
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    • 1997
  • In order to investigate TMD prevalence in malocclusion patients and to study its relationship with occlusal factors, 205 malocclusion patients (M67, F138, 6Y1M-46Y8M) were examined. The following examinations were carried out, Questionnaire personal history, TMD symptoms, and the associated factors Clinical examination : TMJ sound and maximum mouth opening Orthopantomogram : condyle abnormalities, length of Co'-Inc' and Co'-Go', ratio Co'- Inc'/ Co'-Go', and depth of antegonial notch Transcranial view limitation of anterior movement of condyle Model Angle classification, overjet, overbite, midline discrepancy, missing of posterior teeth, posterior crossbite, attrition of palatal cusp of maxillary molars, crowding/spacing The results could be summarized as follows, 1. The prevalence of TMD showed that Helkimo Anamestic Inder(Ai) 0 was $46.8\%$, Ai I was $22.0\%$, Ai II was $31.2\%$ and subjective symptoms increased with aging (p<0.001) and were frequent in females (p<0.05). 2. Flattening ($4.4\%$) was the most frequent condyle abnormality on Orthopantomogram, and $8.3\%$ of subjects showed some abnormalities on Orthopantomogram. 3. The cases with neck and shoulder pain (p<0.001), clenching, lip biting (p<0.01), and headache (p<0.05) showed higher scores of Ai. 4. Angle class II showed high frequency of condylar abnormalities on Orthopantomogram, and subjects whose palatal cusp of maxillary molars had been attrided had the tendency to show high hi scores (p<0.05). The other occlusal factors had nothing to do with the symptoms of TMD. 5. In the cases that 1)the value of Co'-Inc', Co'-Go' or Co'-Inc'/Co'-Go' were low or 2)the differences of Co'-Go' or Co'-Inc'/Co'-Go' between the right and the left were large, condylar abnormalities were frequently obserbed on Orthopantomogram.

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