• Title/Summary/Keyword: Infectious Disease

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Metabolite Profiling of Serum from Patients with Tuberculosis

  • Park, Hee-Bin;Yoo, Min-Gyu;Choi, Sangho;Kim, Seong-Han;Chu, Hyuk
    • Microbiology and Biotechnology Letters
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    • v.49 no.2
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    • pp.264-268
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    • 2021
  • Tuberculosis (TB) is a major infectious disease that threatens the life and health of people globally. Here, we performed a metabolomic analysis of serum samples from patients with intractable TB to identify biomarkers that might shorten the TB treatment period. Serum samples collected at the commencement of patients' treatment and healthy controls were analyzed using the capillary electrophoresis and time-of-flight mass spectrometry metabolome analysis method. The analysis identified the metabolites cystine, kynurenine, glyceric acid, and cystathionine, which might be useful markers for monitoring the TB treatment course. Furthermore, our research may provide experimental data to develop potential biomarkers in the TB treatment course.

Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway

  • Wang, Xin;Dong, Chen-Fang;Shi, Qi;Shi, Song;Wang, Gui-Rong;Lei, Yan-Jun;Xu, Kun;An, Run;Chen, Jian-Ming;Jiang, Hui-Ying;Tian, Chan;Gao, Chen;Zhao, Yu-Jun;Han, Jun;Dong, Xiao-Ping
    • BMB Reports
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    • v.42 no.7
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    • pp.444-449
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    • 2009
  • Different neurodegenerative disorders like prion disease, is caused by protein misfolding conformers. Reverse-transfected cytosolic prion protein (PrP) and PrP expressed in the cytosol have been shown to be neurotoxic. To investigate the possible mechanism of neurotoxicity due to accumulation of PrP in cytosol, a PrP mutant lacking the signal and GPI (CytoPrP) was introduced into the SH-SY5Y cell. MTT and trypan blue assays indicated that the viability of cells expressing CytoPrP was remarkably reduced after treatment of MG-132. Obvious apoptosis phenomena were detected in the cells accumulated with CytoPrP, including loss of mitochondrial transmembrane potential, increase of caspase-3 activity, more annexin V/PI-double positive-stained cells and reduced Bcl-2 level. Moreover, DNA fragmentation and TUNEL assays also revealed clear evidences of late apoptosis in the cells accumulated CytoPrP. These data suggest that the accumulation of CytoPrP in cytoplasm may trigger cell apoptosis, in which mitochondrial relative apoptosis pathway seems to play critical role.

Child Daycare Teachers' Role Perception, Knowledge, Self-confidence and Educational Needs Regarding Infectious Disease Management in Children (아동 감염성 질환관리에 대한 보육교사의 역할인식, 지식, 실천 자신감 및 교육요구도)

  • Back, Seong-Hee;Kim, Jin-Sun
    • The Journal of the Korea Contents Association
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    • v.17 no.7
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    • pp.253-264
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    • 2017
  • The purpose of this study was to investigate role perception, knowledge, self-confidence of practice, and educational needs of child daycare teachers' infectious disease management and to identify their relationship. A correlation study was conducted. Participants were 180 child daycare teachers. Almost eighty percent(78.9%) of participants did not received infectious disease management education. The mean percent of correct answers for infectious disease management knowledge of participants was 52.3% and their self-confidence of practice of infectious disease management was low. However, their role perception of infectious disease management and educational needs were high. Educational needs on 'hand washing and hygiene to prevent infectious diseases', 'blocking transmission of infectious diseases', 'infectious disease specific management and care', and 'infectious disease specific symptoms' were high. Child daycare teachers' role perception of infectious disease was positively correlated with their knowledge and educational needs. Development and evaluation of educational interventions to improve child daycare teachers' infectious disease prevention and management are recommended. Particularly, efforts are needed to improve child daycare teachers role perceptions as infectious disease manager.

A Study on the Architectural Planning of the Ward in Infectious Disease Hospitals (감염병 전문병원의 병동부 건축계획에 관한 연구)

  • Choi, Kwangseok
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.27 no.1
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    • pp.7-15
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    • 2021
  • Purpose: This study analyzed the architectural planning factors of the ward in infectious disease hospitals, such as functional unit planning, ward configurations, spatial compositions & circulation, and detailed architectural planning. Through these, the facility guidelines of infectious disease hospitals are summarized, focusing on the differences from the wards of non-infectious hospitals. Methods: This study was conducted by literature review of research reports, papers, design cases, and guidelines, based on the experiences of field surveys for infectious disease hospitals. Results: The result of this study can be summarized into a few points. 1) Infectious disease hospitals need to establish an operation plan with the concept of continuity of care, as an extension of existing facilities. 2) The types of ward configuration for infectious disease hospitals has many variables, so an appropriate type should be selected according to the hospital's operating policy. 3) Various spatial composition types of the ward can be planned by the arrangement of traffic cores and areas of patient groups. At this time, the main planning considerations are safety, efficiency, and comfort. 4) As elements of the detailed plan, It is necessary to consider the types & dimensions of patient rooms, the relationships between nursing stations & sub-stations, and supplementations of medical support functions & convenience facilities. Implications: Since there are many differences in function from the ward of non-infectious hospitals, appropriate facility guidelines for infectious disease hospital are required.

Nationwide Surveillance Study of Vancomycin-Intermediate Staphylococcus aureus Strains in Korean Hospitals from 2001 to 2006

  • Chung, Gyung-Tae;Cha, Jeong-Ok;Han, Sun-Young;Jang, Hee-Sun;Lee, Kyeong-Min;Yoo, Jae-Il;Yoo, Jeong-Sik;Kim, Hong-Bin;Eun, Soo-Hoon;Kim, Bong-Su;Park, Ok;Lee, Yeong-Seon
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.637-642
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    • 2010
  • We investigated the prevalence and the molecular characteristics of vancomycin-intermediate Staphylococcus aureus (VISA) among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical samples at tertiary or general hospitals participating in a nationwide surveillance program for VISA and vancomycin-resistant Staphylococcus aureus (VRSA) in Korea during an 8-week period in each year from 2001 to 2006. Of 41,639 MRSAs isolated, 37,856 were screened and 169 grew on brain heart infusion agar supplemented with 4 ${\mu}g/ml$ vancomycin. A vancomycin MIC of 4 ${\mu}g/ml$ was confirmed for 33 VISA isolates of the 169 isolates. Eighteen of the 33 isolates were classified as hetero-VISA (hVISA) by the population analysis profile (PAP) method. All VISA isolates were susceptible to linezolid, tigecycline, and quinupristin-dalfopristin. Most VISA isolates (MIC 4 ${\mu}g/ml$) showed a PFGE C pattern with sec, seg, and sei enterotoxin genes, including ST5-SCCmec type II, or a PFGE A pattern with sea, including ST239-SCCmec type III.

Development of a Clinical Nursing Practice Training Simulation Program using Standardized Patient for Nursing Students Focused on Infectious Respiratory

  • Hur, Jung
    • International Journal of Advanced Culture Technology
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    • v.9 no.4
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    • pp.169-179
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    • 2021
  • This study was conducted to develop and apply clinical nursing practice training simulation program using Standardized Patient for Nursing Students focused on infectious respiratory disease. This study is descriptive methodological study. Through prior consideration of documents and educational task of infectious respiratory disease was conducted with interview of clinical specialists of infection control managers. Development of educational task for infectious respiratory disease for Nursing Students went through the content validity. Finally, 10 educational tasks are developed 'knowledge of respiratory infections disease', 'hand washing', 'put on mask', 'lead to put on mask to patients and caregiver', 'intravenous injection via 3way', 'surgical aseptic technique', 'sterilization medical instrument', 'management of contaminated linen', 'infected personnel management manual'. The infectious respiratory disease simulation program was developed based on the ADDIE model and proceeded to 4 steps of analysis, design, development, implementation. The infection control education program included lectures (20 min), skill training (20 min), simulation using standardized patient (20 min), and debriefing (40 min), The collected data were analyzed by descriptive statistics with SPSS program for version 23.0. The results of this study confirmed that the clinical nursing practice training simulation program using standardized patients was effective in infectious respiratory disease of the nursing college students in knowledge of infectious respiratory disease and clinical nursing performance. we found this practical training program for nursing college students to improve knowledge and clinical competency of infection control. we expected that this developed program could be applied to practical training for various infectious control.

Nitric oxide induced by Indian ginseng root extract inhibits Infectious Bursal Disease virus in chicken embryo fibroblasts in vitro

  • Ganguly, Bhaskar;Umapathi, Vijaypillai;Rastogi, Sunil Kumar
    • Journal of Animal Science and Technology
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    • v.60 no.1
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    • pp.2.1-2.5
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    • 2018
  • Infectious Bursal Disease is a severe viral disease of chicken responsible for serious economic losses to poultry farmers. The causative agent, Infectious Bursal Disease virus, is inhibited by nitric oxide. Root extract of the Indian ginseng, Withania somnifera, inhibits Infectious Bursal Disease virus in vitro. Also, Withania somnifera root extract is known to induce nitric oxide production in vitro. Therefore, the present study was undertaken to determine if the inhibitory activity of Withania somnifera against Infectious Bursal Disease virus was based on the production of nitric oxide. We show that besides other mechanisms, the inhibition of Infectious Bursal Disease virus by Withania somnifera involves the production of nitric oxide. Our results also highlight the paradoxical role of nitric oxide in the pathogenesis of Infectious Bursal Disease.