• Title/Summary/Keyword: Inhibition zone

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Doxyclycline and Ciprofloxacin Treatment of Silk to Produce Novel Infection Resistance Biomaterials

  • Park, Hyung-Min;Martin Bide;Matthew Phaneuf;William Quist;Frank Logerfo
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10a
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    • pp.81-82
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    • 2003
  • Two antibiotics, doxycycline (doxy) and ciprofloxacin (cipro) were applied under a variety of conditions to silk, and to silk that had previously been hydrolyzed. FTIR-ATR analyses indicated that the drastic increase in sorption of antibiotics by hydrolyzed silk was attributable to both chemical and conformational changes that occurred on hydrolysis. A high sorption of doxy by hydrolyzed silk did not necessarily give a more infection-resistant material as determined by a zone of inhibition test. Conversely, the same hydrolysis considerably increased both sorption of cipro and zone of inhibition of cipro-treated silk dyed at 65$^{\circ}C$ and 85$^{\circ}C$.

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황련의 증량에 따른 전탕액이 Pseudomonas aeruginosa에 미치는 항균효과에 대한 실험적 연구

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.1 s.22
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    • pp.17-21
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa that induce keratitis. Methods : MIC was measured by dropping to 50 ${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 120g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : MIC on Pseudomonas aeruginosa in Coptidis rhizoma extract compared with quantity(40g, 80g, 120g, 160g) was not showed all samples(100%, 10%, 1%, 0.1%). Conclusions : Coptidis rhizoma extract compared with quantity was not showed anti-bacterial potency on Pseudomonas aeruginosa. The results differ that Coptidis rhizoma extract was showed anti-bacterial potency in 1999 year. Anti-bacterial Potency of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa must be studied.

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus epidermidis (소염 약침액, 황련해독탕, 황련이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.19-25
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture dippear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus epidermidis in Coptidis rhizoma was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

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The Experimental Study on Anti-bacterial Potency of Coptidis rhizoma extract on Staphylococcus aureus & Staphylococcus epidermidis (황련 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.71-76
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by flowing to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was 100% & 10%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The Present author think that Coptidis rhizoma extract must be made a new eye drops.

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The Experimental Study on Anti-bacterial Potency of Jinpi-san on Staphylococcus aureus & Staphylococcus epidermidis. (진피산(秦皮散)이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.1 s.32
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    • pp.195-200
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Jinpi-san on Staphylococcus aureus(S. aureus) and Staphylococcus epidermidis(S. epidermidis) that induce keratitis. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Jinpi-san(100%, 10%, 1%, 0.1 %). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on S. aureus in Jinpi-san was $40{\mu}l$ undiluted solution. 2. MIC on S. epidermidis in Jinpi-san was $20{\mu}l$ undiluted solution. Conclusions : These results indicate that Jinpi-san can be used to cure S. aureus, S. epidermidis that induce eye disease(keratitis). If further study is performed, the use of eye drops will be valuable and beneficial in the clinical medicines.

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EXPERIMENTAL STUDIES ON ANTIBACTERIAL EFFECT OF DISINFECTANTS (수종방부제의 살균효과에 관한 실험적 연구)

  • Yoon, Soo-Han
    • The Journal of the Korean dental association
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    • v.15 no.12
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    • pp.963-968
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    • 1977
  • 1. The purpose of this study is to determine the antibacterial effect of the remedies commonly used in operative dentistry. 2. Experiment were performed against staphylococcus albus which were isolated from different root canals. 3. Isolated bacteria were inoculated on nutrient agar and disk which has been saturated antiseptics were placed on it, then this plate was incubated for 48 hours at 37℃. 4. Inhibition zone were measured around antiseptic disks. 5. The results of 45 cases of experiments showed as follows. Average diameter of inhibition zone around phenol disk was 2.99 mm, camphorated phenol, 0.74 mm, formocresol, 13.46mm, formocresol (Korea), 12.7 mm, eugenol, 4.74mm, thymol, 16.46 mm.

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Pharmacological Screening of Crude Extracts from Medicinal Plants (II)

  • Yook, Chan-Nam;Lee, Jae-Sug;Baek, Seung-Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.4
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    • pp.1004-1009
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    • 2007
  • The effects of crude extracts from medicinal plants on biological activity were investigated. The crude ethanol extract of H. paucistipula inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone at 150 ${\mu}g$/disc) and the dermatophyte Trichophyton mentagrophytes ATCC 28185, (7 mm inhibition zone at 150 ${\mu}g$/disc), and toxic to P388 murine leukaemia cells ($IC_{50}\;2.48\;{\mu}g/ml$ at 75 ${\mu}g$/disk). This crude ethanol extract of H. paucistipula is the strongest antimicrobial and cytotoxic activities against P388 murine leukaemia cells (ATCC CCL 46 P388D1).

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus (소염 약침액, 황련해독탕, 황련이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.87-92
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus aureus in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus aureus in Coptidis rhizoma was 100%, $50\;{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

The Experimental Study on Anti-bacterial Potency of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus & Staphylococcus epidermidis (황련해독탕 약침액과 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.99-103
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture on Staphylococcus aureus & Staphylococcus epidermidis (소염 약침액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.73-77
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture(100%, 10%, 1%, 0.1%). Antibacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture disappear all sample. Conclusions : The present author think that Soyum Herbal-acupuncture must be extract a new method for anti-bacterial potency.