• 제목/요약/키워드: Ionic strength

검색결과 541건 처리시간 0.036초

오골계에서 추출한 Actomyosin 의 ATPase 활성 및 용해도 (ATPase Activity and Solubility of Actomyosin Extracted from Muscle of Silky Fowl)

  • 정인철;문윤희
    • 한국식품영양과학회지
    • /
    • 제23권5호
    • /
    • pp.827-831
    • /
    • 1994
  • Investigation on the extractability, Mg2+-, Ca2+ , EDTA-ATPase activity and solubility of actomyosin prepared from leg and breast muscle of silky fowol were as follows. The extractability of actomyosin in leg and breast muscle was 779mg/100g and 1, 318mg/100g respectively, breast muscle was higher than leg muscle . Mg2+-ATPase activity of actomyosin was high inionic strength 0.02-0.10 and Mg2+ATPase activity of low ionic strength was higher than high ionic strength not related to the part. Ca2+ ATPase activity was high in ionic strength 0.05-0.13, the activity of leg muscle was higher that breast muscle. And EDTA-ATPase activity showed low in low ionic strength and showed high in high ionic strength, and increased greatly depend ionic strength up to 0.4. The solubility of actomyosin was not different in leg and breast muscle , the solution started in KCI concentration of 0.3M and ended in DCI concentration of 0.4M.

  • PDF

Effects of Water Chemistry on Aggregation and Soil Adsorption of Silver Nanoparticles

  • Bae, Sujin;Hwang, Yu Sik;Lee, Yong-Ju;Lee, Sung-Kyu
    • Environmental Analysis Health and Toxicology
    • /
    • 제28권
    • /
    • pp.6.1-6.7
    • /
    • 2013
  • Objectives In this study, we investigated the influence of ionic strength and natural organic matter (NOM) on aggregation and soil adsorption of citrate-coated silver nanoparticles (AgNPs). Methods Time-resolved dynamic light scattering measurements and batch adsorption experiments were used to study their aggregation and soil adsorption behaviors, respectively. Results The aggregation rate of AgNPs increased with increasing ionic strength and decreasing NOM concentration. At higher ionic strength, the AgNPs were unstable, and thus tended to be adsorbed to the soil, while increased NOM concentration hindered soil adsorption. To understand the varying behaviors of AgNPs depending on the environmental factors, particle zeta potentials were also measured as a function of ionic strength and NOM concentration. The magnitude of particle zeta potential became more negative with decreasing ionic strength and increasing NOM concentration. These results imply that the aggregation and soil adsorption behavior of AgNPs were mainly controlled by electrical double-layer repulsion consistent with the Derjaguin-Landau-Verwey-Overbeek theory. Conclusions This study found that the aggregation and soil adsorption behavior of AgNPs are closely associated with environmental factors such as ionic strength and NOM and suggested that assessing the environmental fate and transport of nanoparticles requires a thorough understanding of particle-particle interaction mechanisms.

Effect of Carrier Solutions on Particle Retention in Flow Field-Flow Fractionation

  • 문명희
    • Bulletin of the Korean Chemical Society
    • /
    • 제16권7호
    • /
    • pp.613-619
    • /
    • 1995
  • The influence of carrier solutions on particle retention was studied by varying surfactants and ionic strength in flow field-flow fractionation. Experiments were made with five different submicron polystyrene latex standards at three different types of surfactants and seven different ionic strengths. Departures in particle retention from the general theory were observed. At low ionic strength, it is shown that migrating sample zone is clearly lifted away from the ideal equilibrium height and that the repulsive interaction dominates between the particle and the channel wall. As ionic strength increases up to a certain level, particle retention becomes closer to the general theory. Further increase in ionic strength is shown to prolong the retention. An optimum regime of ionic strength is also suggested with the proper choice of surfactants.

Drug-Biomacromolecule Interaction VIII

  • Kim, Chong-Kook;Yang, Ji-Sun;Lim, Yun-Su
    • Archives of Pharmacal Research
    • /
    • 제7권2호
    • /
    • pp.87-93
    • /
    • 1984
  • The effects of ionic strength and pH on the binding of cefazolin to bovine serum albumin (BSA) were studied by UV difference spectrophotometry. As ionic strength at constant pH and temperature increases, the apparent bining constant decreased but the number of binding sites remained almost constant at 2. The constancy of the number of binding sites with increasing the ionic strength suggests that purely electrostatic forces between BSA and drug do not have great importance in the drug binding, even though there is a decrease in the apparent binding constant. Thus, the effect of ionic strength on the interaction between drug and BSA may be explained by the changes in ionic atmosphere of the aggregated BSA molecules and competitive inhibition by phosphate ions. In addition, the higher apparent binding constant at high ionic strength is explained by conformational changes of BSA from its aggregate forms into subunits. The pH effects on the afinity of interactions indicated that the binding affinity of cefazoline is higher in the neutral region than in the alkaline region. An d at high pH value, the number of binding sites decreased from 2 to 1 because of the conformational change of BSA in the alkaline region.

  • PDF

양액이온 농도 차이가 오이의 생육과 수량에 미치는 영향 (Effect of ionic Strength of Nutrient Solution on Growth and Yield in Cucumber(Cucumis sativus L.))

  • 권병선;정현희;신동영;김학진;임준택;현규환;신정식
    • 한국자원식물학회지
    • /
    • 제16권3호
    • /
    • pp.223-229
    • /
    • 2003
  • 본 연구는 양액재배 오이의 생장과 수량에 나쁜 영향을 주지 않는 적정 양액 농도를 구명하기 위해서 양액의 이온 농도를 달리하였다. Yamazaki의 오이 처방을 기본으로 하여 1/4, 1/2, 3/4, 1, 3/2, 2 배농도를 상이하게 처리하여 생장 특성, 수량 및 질소 함량을 비교하고자 수행하였고 그 결과를 요약하면 다음과 같다. 1. 생육 시기별 초장과 엽수의 증가속도는 저 농도 구인 1/2배 농도에서 가장 빠른 증가속도를 보였다. 2. 양액의 이온농도 차이에 따른 오이의 생육 형질은 유의적인 생장의 차이가 인정되지 않았으며, 생육 시기별 초장, 경장과 엽면적은 1/2배 농도구에서 수치상으로 가장 높은 값을 보였다. 3. 양액의 이온농도 차이에 따른 오이의 과실수량은 유의적인 차이가 인정되지 않았으며 주수에서는 고농도구인 2배 농도가 86.00, 86.75로 가장 높은 값을 보였고, 과실수는 1/4배 농도와 표준 농도에서 가장 낮은 74.25, 85.25를 보였으나 수량에서는 저 농도인 1/2배 농도와 3/4배 농도가 가장 높은 22.60, 22.07kg을 보였다. 4. 질소함량은 유의적인 차이가 인정되지 않았으며, l/2배 농도에서 대체적으로 높은 함량을 보였다. 5. 양액의 이온농도 차이에 따라 정식후 36일 조사 시기의 모든 생육형질과 수량 특성에서 수치상으로 높은 값을 보인 1/2배 농도에서의 상관관계는 초장과 경장은 고도로 유의한 정의 상관관계를, 초장과 엽면적에서는 유의한 정의 상관관계를 나타냈고, 주수와 과실수는 고도로 유의한 정의 상관관계를, 과실수와 수량은 유의한 정 의 상관을 나타냈다.

Ionic Strength 및 공존(共存) 음(陰)Ion이 Zeolite에 의(依)한 중금속(重金屬)의 흡착(吸着)에 미치는 영향(影響) (Effects of Ionic strength and Anion species on Heavy Metal Adsorption by Zeolite)

  • 이정재;박병윤;최정
    • 한국환경농학회지
    • /
    • 제7권2호
    • /
    • pp.96-101
    • /
    • 1988
  • Ionic strength 및 공존(共存) 음(陰)Ion($Cl^-$, $SO^{2-}\;_4$, $ClO^-\;_4$)이 Zeolite에 의(依)한 중금속(重金屬)의 흡착(吸着)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같다. Ionic strength별(別) Zeolite에 의(依)한 Cd, Cu 및 Zn의 흡착등온선(吸着等溫線)은 Freundlich 식(式)에 잘 부합(符合)되었다. Ionic strength가 증가(增加)할수록 중금속(重金屬)의 흡착량(吸着量)은 3종(種)의 서로 다른 음(陰)Ion이 공존(共存)하여도 모두 감소(減少)하는 경향(傾向)이었다. Background salt의 음(陰)Ion 종류별(種類別) 중금속(重金屬) 흡착량(吸着量)은 $ClO^-\;_4>SO^{2-}\;_4>Cl^-$의 순(順)이었으며 흡착평형용액내(吸着平衡溶液內)에서 음(陰)Ion은 Ligand로 작용(作用)하여 중금속(重金屬) Ion들과 Complex를 형성(形成)하는 능력(能力)에 차이(差異)가 있음이 인정(認定)되었다.

  • PDF

Drug Diomacromolecule interaction IX

  • Kim, Chong-Kook;Won, Young-Han;Kim, Sang-Nim
    • Archives of Pharmacal Research
    • /
    • 제7권2호
    • /
    • pp.95-99
    • /
    • 1984
  • Binding of sulfaethidole to bovine serum albumin (BSA) was studied by circular dichroism. The effects of pH and ionic strength on the binding of sulfaethidole to BSA were investigated. It was found that one primary binding site on the BSAM was capable of inducing optical activity in the presence of sulfaethidole. Enhancement of the induced ellipticity of sulfaethidole upon addition to BSA was not much affected by the change of pH and ionic strength. Taking the effects of pH and ionic strength into consideration, it seems that the binding of sulfaethidole to BSA was not much affected by electrostatic and ionic interactions. Therefore, it might be assumed that the binding was mainly due to the hydrophobic interactions. Sulfaethidole seems to be a reasonable CD probe for the study of hydrophobic drug interactions.

  • PDF

개고기 다리와 가슴 근육에서 추출한 근원섬유 단백질의 특성 (Characteristics of Myofibrillar Protein Extracted Leg and Breast Muscles of Dog Meat)

  • 박경숙;윤동화;정인철
    • 동아시아식생활학회지
    • /
    • 제16권4호
    • /
    • pp.453-457
    • /
    • 2006
  • This study investigated the extractability, solubility, Mg$^{2+}$-, Ca$^{2+}$- and EDTA-ATPase activity of actomyosin prepared from leg and breast muscle of dog meat. The actomyosin extractability of breast muscle(2,100.6 mg/l00 g) was higher than that of leg muscle(500.8 mg/l00 g). The Mg$^{2+}$-ATPase activity of actomyosin had a high ionic strength of 0.02$\sim$0.05 M KCI and did not differ between leg and breast muscle. The Ca$^{2+}$-ATPase activity of actomyosin had a high ionic strength of 0.02$\sim$0.10 M KCI and leg muscle had a higher level of Ca$^{2+}$-ATPase activity than breast muscle did. The EDTA-ATPase activity was lower in low ionic strength and showed higher in high ionic strength, and increased sharply with increasing ionic strength up to 0.3 M KCI. The solubility of actomyosin did not differ between leg and breast muscle, and the solubility started and ended at KCI concentrations of 0.35 M and 0.4 M, respectively.

  • PDF

Sorption of Se(-II) on illite, MX-80 bentonite, shale, and limestone in Na-Ca-Cl solutions

  • Walker, Andrew;Racette, Joshua;Saito, Takumi;Yang, Tammy (Tianxiao);Nagasaki, Shinya
    • Nuclear Engineering and Technology
    • /
    • 제54권5호
    • /
    • pp.1616-1622
    • /
    • 2022
  • Selenium has been identified as an element of interest for the safety assessment of a deep geological repository (DGR) for used nuclear fuel. In Canada, groundwaters at DGR depths in sedimentary rocks have been observed to have a high ionic strength. This paper examines the sorption behavior of Se(-II) onto illite, MX-80 bentonite, Queenston shale, and argillaceous limestone in Na-Ca-Cl solutions of varying ionic strength (0.1-6 mol/kgw (m)) and across a pH range of 4-9. Little ionic strength dependence for Se(-II) sorption onto all solids was observed except that sorption at high ionic strength (6 m) was generally slightly lower than sorption at low ionic strength (0.1 m). Illite and MX-80 exhibited the expected results for anion sorption, while shale and limestone exhibited more constant sorption across the pH range tested. A non-electrostatic surface complexation model successfully predicted sorption of Se(-II) onto illite and MX-80 using the formation of an inner-sphere surface complex and an outer-sphere surface complex. Optimized values for the formation reactions of these surface species were proposed.

피부의 전기적 저항에 대한 이론적 모델 (Theoretical Model for the Electrical Resistance of Skin)

  • 오승열
    • Journal of Pharmaceutical Investigation
    • /
    • 제26권3호
    • /
    • pp.207-213
    • /
    • 1996
  • The kinetic change of electrical resistance of hairless mouse skin as a function of ionic strength of the bathing medium was determined from impedance measurements. After increasing (decreasing) the ionic strength of the bathing medium, resistance decreased (increased) continuously with time, finally reaching an equilibrium value. We have modelled this process, using nonsteady-state diffusion kinetics. The results show semi-quantitative correlation between theoretically derived and experimentally obtained values. Overall, this work provides further mechanistic insight into ion-conduction through the skin.

  • PDF