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Resistance to Two Leaf Spot Diseases of Pepper Genetic Resources Introduced from Mexico and Nepal (멕시코와 네팔도입 고추 유전자원의 두 가지 점무늬병에 대한 저항성)

  • Jo, Eun-Hyeong;Kim, Jeong-Hoon;Jun, Su-Kyung;Lee, Ji-Seon;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.23
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    • pp.43-51
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    • 2005
  • Pepper genetic resources consisting of introductions from Mexico and Nepal and susceptible and resistant controls were tested for resistance to gray leaf spot and to bacterial spot by serially inoculating the two disease pathogens, Stemphylium spp. first and Xanthomonas campestris pv. vesicatoria next, with application of fungicide after evaluation of resistance to gray leaf spot first. KC866, KC872, KC902, KC905 were resistant to gray leaf spot in addition to known resistance sources, KC43, KC47, KC220, KC319, KC320, KC380. KC897 was on the top of the resistance sources list, even better than KC177(163192), and was followed by KC889, KC896, KC898, all of which were introductions from Nepal.

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Evaluation of Pepper Germplasm and Cultivar Introduction (고추 보존(保存) 유전자원(遺傳資源)의 특성평가(特性評價)와 품종도입(品種導入))

  • Lee, Woo Sung;Kim, Byung Soo;Kim, Sang Gee;Park, Kyu Hwan;Kwon, Young Seok
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.35-44
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    • 1990
  • Sixty two lines of pepper including Korean local cultivars and introductions were planted out to the experiment farm of Kyungpook National University for seed increase, and 16 plant and fruit characters including days to flower were recorded. In days to flower, KC85(Chilgok Dongmyung) and KC116(PI308791), KC154(Cherry Sweet), and KC185(Choseongochu) were the earliest with 83 days, and KC139(Serrano chili) and KC103(PI297488) were the latest with 126 days. In fruit detachment force, KC85(Chilgok dongmyung) was the tenderest with 0.45kg and KC162(PI22444) was the toughest with 3.34kg. In powdery mildew incidence, KC10(Pimiento), KC23(PI224423), KC51(PI257044), KC56(PI257053), KC126(PI358812), KC119(PI322719), KC143(Emerald Giant), KC148(VR-2), KC163(PI224445), KC163(Papari Mild) were the least diseased. Anthracnose was least on KC39(Serrano Chili). Collection and introduction of pepper germplasm was continued. Fourteen Korean local cultivars and 25 foreign cultivars including 2 wild species were additionally collected or introduced.

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A Search for Sources of Resistance to Powdery Mildew (Leveillula taurica (Lév.) Arn) in Pepper (Capsicum spp.) (고추 흰가루병에 대한 저항성 재료 탐색)

  • Lee, Ok-Hee;Hwang, Hee-Sook;Kim, Joo-Young;Han, Jeung-Hye;Yoo, Young-Shin;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.7-11
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    • 2001
  • A total of 238 accessions of peppers (Capsicum spp.) were evaluated for resistance to powdery mildew (Leveillula taurica (Lev.) Arn) in 1998-1999. KC604, 605, 606 and 608 of C. baccatum, KC616 of C. chinense, and KC638, 640, 641, 642, 643 and 644 of C. pubescens were highly resistant and remained disease-free. KC47-1 (PI244670), KC319-1, KC545, KC320 showed only mild infection compared with susceptible control group, 'Chungok' and others. Therefore, they were considered moderately resistant. Among them, KC47-1, KC319-1 and KC320 are sources of resistance to gray leaf spot caused by Stemphylium spp. while KC47-1 is also resistant to bacterial leaf spot (Xanthomonas campestris pv. vesicatoria (Doidge) Dye). Therefore, they may be used in breeding for multiple resistance to those diseases.

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Modigication of host cells and Expression of Recombinant E. coli trp plasmids for the increased Production of Tryptophan in Klebsiella pneumoniae (Klebsiella pneumoniae에서 트립토판 생산증대를 위한 숙주개발 및 재조합 trp plasmid의 발현)

  • 지연태;홍광원;박장현;이세영
    • Korean Journal of Microbiology
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    • v.25 no.1
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    • pp.46-51
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    • 1987
  • In order to increase the production of tryptophan by maximizing expression of recombinant trp plasmid, Klebsiella pneumoniae KC 105(pheA tyrA trpE trpR tyrR) was genetically modified. KC 107, inosine monophospate(IMP) auxotroph from KC 105 and KC 108, histidine(His) auxotroph from KC 107 were also derived respectively to increase phosphoribosylpyrophosphate(PRPP) production which is required for tryptophan biosynthesis. From KC 107 phosphoribosylpyrophosphate consumption which is required for tryptophan biosynthesis. From KC 107 and KC 108, KC 109 and KC 110, both arginine auxotrophs were derived respectively. To investigate the expression of recombinant trp plasmid in the selected K. pneumoniae mutants, the auxotrophic mutants were transformed with recombinant trp plasmids pSC 101-$trpE^{FBR}$, pSC 101-trpL(.DELTA.att) $trpE^{FBR}$ (pSC 101-trp-AF). Amount of tryptophan produced and activities of tryptophan synthase of $trpR^{-}$ mutant (KC 100) and $tyrR^{-}$ mutnat(KC 105) containing recombinant plasmid pSC 101-trp operon were increased by 30-40% as compared with KC 99(pheA tyrA trpE) containing recombinant plasmid pSC 101-trp operon. Activities of tryptophan synthase and production of tryptophan of KC 108 ($His^{-}$) and KC 109($Arg^{-}$) containing recombinant plasmid pSC 101-trp operon were increase by two-fold as compared with KC 107 containing pSC 101-trp operon.

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Evaluation and Seed Increase of Sources of Resistance to Phytophthora capsici in Pepper (고추 역병 저항성 재료의 특성평가와 종자 증식)

  • Kim, Dong-Woo;Kim, Byung-Soo;Bae, Su-Yeon;Han, Jeong-Hye
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.83-90
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    • 2002
  • Resistance to Phytophthora Capsici and horticultural characteristics of selections from the resistance sources such as PI123469, PI201234, PI201232, AC2258 (=Line 29), CM334, KC268, KC358, KC820, KC821, KC822, KC823 (Line 29), KC462, KC463, KC464 were evaluated and recorded in the process of seed increase. Selections of PI123469, PI201234, PI201232, AC2258, KC823 (Line 29 = AC2258) of them showed the highest level of resistance. A considerable difference in the level of resistance was observed between lines selected from the same sources. The variation between selections derived from the same source was thought to be attributable to natural cross-pollination that may have occurred during the seed increase. Therefore, securing self-pollinated seed by wrapping the flower buds before anthesis would be necessary to obtain genetically pore resistant lines. The next alternative would be to increase the seed in small net houses for single line or field cage.

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Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers (고추 세균성점무늬병 저항성 유전자원과 그 주요 특성)

  • Byeon, Si-Eun;Abebe, Alebel Mekuriaw;Jegal, Yoon-Hyuk;Wai, Khin Pa Pa;Siddique, Muhammad Irfan;Mo, Hwang-Sung;Yoo, Hee Ju;Jang, Kil-Su;Hwang, Ji-Eun;Jeon, Su-Gyeong;Lee, Su-Heon;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.779-789
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    • 2016
  • A total of 33 accessions of pepper (Capsicum spp.), including previously reported and newly discovered sources of resistance to bacterial spot caused by Xanthomonas euvesicatoria, were evaluated for their resistance to bacterial spot. The selected accessions were then grown and their horticultural characteristics were recorded. In a test for hypersensitive resistance (HR) to four races (P1, P3, P7, P8) of the pathogen found in Korea, KC00939 and Chilbok No.2, which carry the Bs2 gene, exhibited a hypersensitive response to all four races, as expected. Chilbok No.3, which carries the Bs3 gene, showed a hypersensitive reaction to race 1 and 7, as expected. KC00939 exhibited a high ASTA color value and tolerance to multiple infections from a viral complex of Cucumber mosaic virus (CMV) and Broad bean wilt virus (BBWV). Thus, this accession represents a promising genetic resource for breeding cultivars with multiple disease resistance and strong red coloration. KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127, KC01704, and KC00995 did not exhibit hypersensitivity but showed a high level of general resistance when evaluated by spray inoculation. KC01617, KC01760, KC01779, KC01137, KC01704, and KC01777 are newly identified sources of resistance to bacterial spot. The previously and newly identified sources of resistance to bacterial spot evaluated in this study, including information about their resistance to CMV and BBWV complex in the field, the contents of pungent and sweet taste components, and the color values of dry fruits, will be useful for breeding pepper cultivars with resistance to bacterial spot.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

Resistance to Bacterial Wilt and to Phytophthora Blight of Genetic Resources of Pepper Introduced from Mexico and Nepal (멕시코와 네팔에서 도입한 고추 유전자원의 풋마름병 및 역병 저항성)

  • Koh, Bo-Whan;Kim, Jeong-Hoon;Jun, Su-Kyung;Lee, Ji-Seon;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.23
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    • pp.33-41
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    • 2005
  • Above 130 accessions of pepper consisting of 50 introductions from Mexico and Nepal, and resistant and susceptible controls were tested for resistance to bacterial wilt and to Phytophthora root rot at seedling stage by artificial inoculation. Aa the results, KC897, KC939, KC936 were newly found resistant to bacterial wilt in addition to already known resistance sources such as KC126, KC350, KC351, KC353. No new sources of resistance to Phytophthora root rot were found among the introductions from Mexico and Nepal.

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A STUDY ON THE PERFORMANCE OF KHAKI CAMPBELL, DESI AND KHAKI CAMPBELL X DESI DUCKS WITH AND WITHOUT EXTRA FEEDING UNDER RURAL CONDITION OF BANGLADESH

  • Rashid, N.;Barua, A.;Bulbul, S.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.4
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    • pp.375-378
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    • 1995
  • Three month old 600 ducklings of Khaki Campbell/KC Desi and KC X Desi were reared for a period of 13 months in the villages surrounding Bangladesh Agricultural University in order to study the economic traits of duck with and without extra feed to scavenging system of rearing. KC attained earlier sexual maturity followed by KC X Desi and Desi ducks and crossbred showed closeness to sire breed for this trait, KC X Desi found heavier and produce more eggs than their parental breeds. KC showed higher hatchability followed by KC X Desi and Desi ducks were more resistant followed by KC X Desi and KC to diseases. Extra feeding were found more beneficial to ducks irrespective of breed and types.

Sources of Resistance to Bacterial Wilt Found in Vietnam Collections of Pepper (Capsicum annuum) and Their Nuclear Fertility Restorer Genotypes for Cytoplasmic Male Sterility

  • Tran, Ngoc Hung;Kim, Byung-Soo
    • The Plant Pathology Journal
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    • v.28 no.4
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    • pp.418-422
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    • 2012
  • Eleven hot pepper accessions collected in Vietnam showed stable resistance to bacterial wilt as well-known resistance sources, MC4 and MC5, in repeated inoculation tests with different Ralstonia solanacearum isolates conducted from 2004 to 2010. Seven of these accessions (specifically KC981, KC1006, KC1021, KC1027, KC1045, KC1050, and KC1055) resulted in stable male sterile F1 plants in the crosses with a cytoplasmically male sterile (CMS) Chilseong (CMS-A, Srfrf ), and therefore, they were maintainers (CMS-B) with a genotype of Nrfrf. The rest (KC980, KC995, KC999, and KC1009) produced stable male fertile F1 plants in the crosses, and therefore, were restorers (CMS-C) with a genotype of N(S)RfRf. Therefore, the maintainer and restorer sources of resistance may be used in preference in breeding maternal (CMS and their maintainers) and paternal parents (restorers) for resistance to bacterial wilt, respectively, in the hybrid breeding system utilizing cytoplasmic male sterility.