• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.1019-1025
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• 2014

This study was conducted to observe the effects of crossbreeding and gender on the carcass traits and meat quality of Korean Native Black Pig (KNP) and $KNP{\times}Duroc$ crossbred ($KNP{\times}D$). A total of 50 pigs comprising seven KNP barrows, eight KNP gilts, twenty $KNP{\times}D$ barrows and fifteen $KNP{\times}D$ gilts were used in this study. Animals were reared in the same housing condition with same feed diet for six months prior to slaughter. After an overnight chilling, the carcasses were graded, and samples of Musculus longissimus dorsi were obtained for meat quality analysis. The slaughter and carcass weights and dressing percentage of $KNP{\times}D$ were higher (p<0.001) than those of KNP. The slaughter and carcass weights and backfat thickness of barrows were higher (p<0.01) than those of gilts. There were no significant difference in carcass conformation and quality grade between KNP and $KNP{\times}D$ as well as barrow and gilt. Fat content of $KNP{\times}D$ was higher (p<0.001) than that of KNP. Fat content of barrow was higher (p<0.001) than that of gilt. There was interaction between crossbreeding and gender on the fat content. KNP gilt showed higher fat content than KNP barrow whereas $KNP{\times}D$ barrow showed higher fat content than $KNP{\times}D$ gilt. Lightness, redness, yellowness, chroma and hue angle values and color preference of meat of $KNP{\times}D$ were lower (p<0.001) than those of KNP. Redness, yellowness and chroma values of meat of barrow were lower (p<0.05) than those of gilt. It is concluded that crossbreeding KNP with Duroc increases carcass productivity and meat fat but decreases meat color values and preference. Crossbreeding of KNP with Duroc produces a better fat deposition in meat of barrows than in gilts.

• Journal of Animal Science and Technology
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• 제60권3호
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• pp.6.1-6.5
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• 2018

Background: The meat quality of Korean native pigs (KNP) and crossbred pigs (LYD; Landrace ${\times}$ Yorkshire ${\times}$ Duroc) was examined to generate data useful for selecting native pigs for improved pork production. Methods: Fifty Korean native pigs (KNP) and 50 crossbred pigs (LYD) were tested. Loin samples (M. longissimus dorsi) of the two breeds were analyzed to determine meat quality and sensory properties. Result: KNP had a higher moisture content than LYD (p < 0.05); however, it had significantly lower crude fat and ash content than that of LYD (p < 0.001). KNP had significantly higher shear force than LYD (p < 0.01). KNP also showed significantly higher cooking loss than LYD (p < 0.05). KNP had a lower $L^*$ value than LYD (p < 0.05); however, it had a markedly higher $a^*$ and $b^*$ value than LYD (p < 0.001). KNP showed significantly higher linoleic acid, linolenic acid, and arachidonic acid content than LYD (p < 0.05). Although KNP had significantly better flavor and overall palatability than LYD, it was less tender than LYD (p < 0.01). Conclusion: KNP had a markedly higher $a^*$ value than LYD. KNP had significantly higher shear force than LYD. The total unsaturated fatty acid content was higher in KNP than in LYD.

• 한국축산식품학회지
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• 제25권3호
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• pp.322-327
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• 2005

본 연구는 재래흑돼지와 개량종 돼지들과의 도체 및 육질 특성을 비교 평가하여 표준화를 통해 부가가치를 높여 품질을 고급화 할 수 있는 기초자료를 제시할 목적으로 재래흑돼지와 개량종 돼지인 듀록종(DD), 랜드레이스종(LL), 요크셔종(YY) 그리고 삼원교잡종(LYD)을 시험에 공시해 품종간 처리구별 도체 및 육질 성적을 비교 분석하였다. 본 연구 결과, 재래흑돼지의 생체중, 도체중, 거래 정육 생산량, 가죽, 지방 및 뼈의 생산량이 개량종 처리구에 비해 유의적으로 낮게 나타났으며, 재래흑돼지의 도체율과 정육률도 개량종 4처리구에 비해 유의적으로 낮은 경향이었으나(p<0.05), 총지방 생산율, 가죽, 뼈, 신 및 신지방의 생산율은 재래흑돼지가 개량종에 비해 유의적으로 높았다(p<0.05). 재래흑돼지는 개량종과 비교해 모든 부위에서 낮은 정육량을 보였으며, 품종간에는 YY종이 가장 높았고, 다음 LYD종이 높았으며, KNP가 가장 낮았다. 삼겸살 정육량은 KNP, YY종, LYD종이 각각 6.12, 9.43, 9.52 kg으로 나타났으며, 재래흑돼지의 삽겹살 정육률은 $11.11\%$이었으며, 개량종은 $11.5\~12.2\%$를 나타내었다. 또한 전단가는 품종간 유의적인 차이를 보이지 않았으나 보수력과 가열 감량은 재래흑돼지가 낮은 경향을 보인 (p<0.05) 반면, 다즙성은 근내 지방함량이 높은 관계로 유의적으로 높았다(p<0.05), 육색의 $a^{*}$값은 KNP가 가장 높았고 (p<0.05), DD종이 가장 낮았다(p<0.05).

• Journal of Animal Science and Technology
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• 제57권3호
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• pp.12.1-12.5
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• 2015

Background: The Korean native pig (KNP) is generally thought to have come from northern China to the Korean peninsula approximately 2000 years ago. KNP pigs were at the brink of extinction in the 1980s, since then efforts have been made to restore the breed by bringing together the remaining stocks in South Korea. As a result, KNP was registered as a breed in 2006. To find additional breed-specific markers that are distinct among pig breeds, variations in O-linked N-acetylglucosamine transferase (OGT) were investigated. OGT is located on chromosome X and catalyzes the post-translational addition of a single O-linked-${\beta}$-N-acetylglucosamine to target proteins. Findings: Length polymorphism in the intron 20 of OGT was identified. The intron 20 of OGT from Duroc, Landrace, and Yorkshire breeds was 281-bp longer than that from either KNP or Chinese Meishan pigs. The difference between the Western pig breeds (BB genotype) and KNP or Meishan pigs (AA genotype) was due to an inserted 276-bp element and the 5-bp ACTTG. Conclusions: The polymorphism in OGT identified in this study may be used as an additional marker for determining the breed of origin among Meishan and the Western pig breeds. The length polymorphism suggests that the locus near OGT is not fixed in KNP. This marker would be relevant in determining the breed of origin in crossbred pigs between KNP pigs with known genotypes and the Western pig breeds with BB genotypes, thus confirming the contribution of the X chromosome from each breed.

• 한국축산식품학회지
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• 제21권3호
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• pp.183-191
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• 2001

Comparisons between the Korean native pig(KNP: 75kg of slaughter weight and 240 days of age) versus Landrace(110kg of slaughter and 180 days of age) in dressing and lean meat percentages, compositions of fatty acids and amino acids and sensory evaluations on longissimus muscle are as below. Compared with Landrace, KNP had smaller percentages in dressing and lean meat percentages and a smaller backfat thickness. In retail cuts, KNP had greater percentages in shoulder butt, belly and picnic shoulder and smaller percentages in spareribs, ham and loin muscle. The KNP had greater percentages in crude fat, palmitic(C16:0) and linolenic(C18:3) acids and smaller percentages in oleic(C18:1) and linoleic(C18:2) acids, but total cholesterol content and ratios of saturated and unsaturated fatty acids and essential fatty acids did not differ between the two breeds. In amino acid composition, KNP had a greater percentage in lysine, but smaller percentages in most other amino acids. The KNP had a greater a*(redness) value in longissimus muscle(LM) color and a greater L*(whiteness) value in attached backfat color. Moreover, sensory evaluations on cooked LM showed that KNP had greater flavor, tenderness and springiness, which resulted in a greater overall acceptability. In summary, KNP, compared with Landrace, had smaller dressing and lean meat percentages, a smaller backfat thickness, greater percentages in popular retail cuts, greater percentages in intramuscular fat and an $\omega$-3 component linolenic acid, with no difference in cholesterol content, which, altogether, is considered to be more beneficial for human health.

• 한국축산식품학회지
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• 제30권3호
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• pp.403-409
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• 2010

Accurate methods for the identification of closely related species or breeds in raw and processed meats must be developed in order to protect both consumers and producers from mislabeling and fraud. This paper describes the development of DNA markers for the discrimination and improvement of Korean native pig (KNP) meat. The KIT gene is related to pig coat color and is often used as a candidate marker. A 538 bp fragment comprising intron 19 of the pig KIT gene was amplified by PCR using specific primers, after which the PCR amplicons of a number of meat samples from KNP and three major improved breeds (Landrace, Duroc and Yorkshire) were sequenced in order to find a nucleotide region suitable for PCR-RFLP analysis. Sequence data showed the presence of two nucleotide substitutions, g.276G>A and g.295A>C, between KNP and the improved pig breeds. Digestion of KIT amplicons with AccII enzyme generated characteristic PCR-RFLP profiles that allowed discrimination between meats from KNP and improved pig. KNP showed three visible DNA bands of 264/249, 199, and 75 bp, whereas DNA bands of 249, 199, and 90 bp were detected in the three improved pig breeds. Therefore, the 75 bp DNA fragment was specific only to KNP, whereas the 90 bp DNA fragment was specific to the improved breeds. The breed-specific DNA markers reported here that target the KIT gene could be useful for the identification of KNP meat from improved pig meats, thus contributing to the prevention of falsified breed labeling.

• Molecules and Cells
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• 제43권8호
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• pp.728-738
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• 2020

Time and cost-effective production of next-generation sequencing data has enabled the performance of population-scale comparative and evolutionary studies for various species, which are essential for obtaining the comprehensive insight into molecular mechanisms underlying species- or breed-specific traits. In this study, the evolutionary and functional analysis of Korean native pig (KNP) was performed using single nucleotide polymorphism (SNP) data by comparative and population genomic approaches with six different mammalian species and five pig breeds. We examined the evolutionary history of KNP SNPs, and the specific genes of KNP based on the uniqueness of non-synonymous SNPs among the used species and pig breeds. We discovered the evolutionary trajectory of KNP SNPs within the used mammalian species as well as pig breeds. We also found olfaction-associated functions that have been characterized and diversified during evolution, and quantitative trait loci associated with the unique traits of KNP. Our study provides new insight into the evolution of KNP and serves as a good example for a better understanding of domestic animals in terms of evolution and domestication using the combined approaches of comparative and population genomics.

• Journal of Animal Science and Technology
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• 제49권1호
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• pp.89-98
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• 2007

본 연구는 재래종 흑돼지육과 개량종 돼지육의 냉장저장중 향기성분 및 패턴을 비롯한 품질을 비교하고자 실시하였다. 사육기간이 모두 6개월인 70kg의 재래흑돼지 5두와 103kg의 개량종 돼지(Landrace×Yorkshire) 5두로부터 발골한 뒷다리육(M. Biceps femoris)을 진공 포장하여 2±0.2℃에 12일 동안 저장하였다. 일반성분을 보면 재래흑돼지육의 수분 함량이 개량종 돼지육 보다 낮았으나(p<0.05), 조단백질 및 조회분 함량은 높았다(p<0.05). pH는 재래흑돼지육이 낮았고(p<0.05), 그에 따라 가열감량은 높았다(p<0.05). 표면육색을 보면 저장기간 동안 재래흑돼지육의 a*, b* 값이 개량종 돼지육 보다 높았다(p<0.05). TBARS는 저장 8일부터, 전단력은 저장 8일까지 재래흑돼지육이 높았다(p<0.05). SPME-GC/MS에 의한 가열육의 향기성분에서 일부 차이를 보였으나, 전자코에 의한 향기패턴 차이는 없었다. 따라서 재래흑돼지육은 색깔이 붉고 짙으며, 가열감량이 높았고 조직감도 단단하였다. 또한 기계적으로 향기특성의 차이를 구별하지 못했지만, 관능검사상 재래흑돼지육이 우수한 기호도를 나타내었다.

• 한국발생생물학회지:발생과생식
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• 제19권2호
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• pp.79-84
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• 2015

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1263-1269
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• 2014

Comprehensive information on genetic diversity and introgression is desirable for the design of rational breed improvement and conservation programs. Despite the concerns regarding the genetic introgression of Western pig breeds into the gene pool of the Korean native pig (KNP), the level of this admixture has not yet been quantified. In the present study, we genotyped 93 animals, representing four Western pig breeds and KNP, using the porcine SNP 60K BeadChip to assess their genetic diversity and to estimate the level of admixture among the breeds. Expected heterozygosity was the lowest in Berkshire (0.31) and highest in Landrace (0.42). Population differentiation ($F_{ST}$) estimates were significantly different (p<0.000), accounting for 27% of the variability among the breeds. The evidence of inbreeding observed in KNP (0.029) and Yorkshire (0.031) may result in deficient heterozygosity. Principal components one (PC1) and two (PC2) explained approximately 35.06% and 25.20% of the variation, respectively, and placed KNP somewhat proximal to the Western pig breeds (Berkshire and Landrace). When K = 2, KNP shared a substantial proportion of ancestry with Western breeds. Similarly, when K = 3, over 86% of the KNP individuals were in the same cluster with Berkshire and Landrace. The linkage disquilbrium (LD) values at $r^2_{0.3}$, the physical distance at which LD decays below a threshold of 0.3, ranged from 72.40 kb in Landrace to 85.86 kb in Yorkshire. Based on our structure analysis, a substantial level of admixture between Western and Korean native pig breeds was observed.