• The Korean Journal of Food And Nutrition
• /
• v.5 no.2
• /
• pp.137-143
• /
• 1992

As a research for developing biofertilizers, Klebsiella oxytoca, an associative nitrogen fixer in the rhizosphere of rice plant in the soil of paddy field, was subjected to molecular breeding. The results obtained were as followings. 1). By transforming pbIC71A, Nif A overproducing plasmid, into Klebsiella oxytoca NGl3, Klebsiell6f oxytoca SH3l, and Klebsiella oxytoca SH161, nitrogenase activities in the absence of nitrogen source in the medium were increased 6.4, 17.2, and 13.5 times, respectively, in comparison with the parent strains. 2). Nitrogenase activity of Klebgiella oxytoca NGl3, Klebsiella oxytoca SH3l, and Klebsiella oxytoca SH161 was completely repressed In the presence of 15mM NH4+. But, nitrogenase activities of Klebsiella oxytoca NGl3/PMC71A, Klebsiella oxytoca SH3l /PMC71A, and Klebsiella oxytoca SH 161/pMC714 harboring PMC71A, were 13.7%, 7.7%, and 6.2% of the nitrogenase activities in the absence of nitrogen source in the medium, respectively.

• Korean Journal of Microbiology
• /
• v.36 no.1
• /
• pp.58-63
• /
• 2000

A bacterial isolate capable of degrading benzoate was selected from wastewater of Yocheon industrial complex and examined its biochemical characteristics and fatty acid composition. The isolate was identified as Klebsiella oxytoca strain C302. The strain C3O2 degraded catechol, protocatechuate, and 4-hydroxybenzoate as well as benzoate. The strain grew on and degraded 0.5 to 1.0 mM catechol most actively in MM2 medium at pH 7.0 and $30^{\circ}C$.

• Korean Journal of Veterinary Service
• /
• v.44 no.4
• /
• pp.305-308
• /
• 2021

A female Chinese hamster with unknown age was referred for acute onset of anorexia, depression, and large cyst from the head to body. After referring, the patient died shortly. During necropsy, severe hemorrhage in the cyst, multiple mass on liver, and transformed right kidney were found. The infection was confirmed by cytology, cultures and PCR of 16S ribosomal RNA gene. This report describes a first case of naturally occurred systemic Klebsiella oxytoca infection in a household Chinese hamster.

• Korean Journal of Clinical Laboratory Science
• /
• v.51 no.4
• /
• pp.428-435
• /
• 2019

The rapid increase and dissemination of carbapene mases, such as Klebsiella pneumoniae carbapenemase (KPC), has become a major problem within the field of healthcare-related infection. There are few antibiotics to treat carbapenem-resistant Enterobacteriaceae (CRE) infections, so the identification of resistant bacterial mechanisms is critical to initiate infection control and conduct epidemiological research. A rapid and effective method for detecting KPC-producing bacteria is needed to avoid therapeutic failures and introduce measures to prevent and control the dissemination of these multi-resistant bacteria. During the study period, 31 isolates (seven isolates of Acinetobacter spp., six isolates of Morganella morganii, five isolates of Pseudomonas aeruginosa, five isolates of Proteus mirabilis, one isolate of Proteus vulgaris, two isolates of Enterobacter cloacae, one isolate of Enterobacter aerogenes, one isolate of Klebsiella pneumoniae, one isolate of Klebsiella oxytoca, one isolate of Serratia marcescens and one isolate of Escherichia coli) were identified by the VITEK. Gram negative rod bacteria were the most frequently isolated from urine (35.5%), blood (19.4%), sputum (16.1%), pus (9.7%), ascitic fluid (9.7%), tracheal aspirates (6.5%) and bile juice (3.2%). Analysis using the PCR method identified the bla_KPC gene in the K. oxytoca1 strain, but the bla_IMP, bla_VIM and bla_OXA-48 genes are not amplified. In conclusion, diagnosis using the PCR method can accurately and quickly diagnose KPC, thus establishing quick preventive measures to prevent the spread of KPC in hospitals.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.21 no.2
• /
• pp.141-146
• /
• 2018

Nowadays, Klebsiella oxytoca is described as a causative organism for antibiotic-associated hemorrhagic colitis (AAHC). Here we report two cases of pediatric AAHC, from which K. oxytoca was cultured after starting amoxicillin-clavulanate or amoxicillin treatment. The patients developed severe abdominal pain and a large amount of bloody diarrhea. K. oxytoca was obtained in intestinal fluid culture of a boy through the colonoscopy. On the other hand, colonic tissue culture and intestinal fluid culture were negative of the other patient. K. oxytoca was detected in stool culture when he was admitted. These cases showed characteristic endoscopic findings of segmental hemorrhagic colitis, and both boys recovered spontaneously within 2-3 days after they stopped taking the antibiotics. Therefore, in children who develop relatively large amount of bloody diarrhea after antibiotic treatment, we should consider AAHC caused by K. oxytoca.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.3 no.1
• /
• pp.48-49
• /
• 1998

A protocol for the transformation of Klebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain high transformation efficiency. The highest efficiency of 1.6$\times$106 transformants per $\mu\textrm{g}$ DNA(pBR322) could be obtained by electroporation of K. oxytoca cells prepared at the OD600 of 0.2 with 1.25$\mu\textrm{g}$ DNA at the filed strength of 2.5kV, the parallel resistance of 200$\Omega$ and capacitance of 25$\mu$F.

• Korean Journal of Soil Science and Fertilizer
• /
• v.46 no.2
• /
• pp.112-116
• /
• 2013

The aim of the present study was to isolate phosphate solubilizing bacteria (PSB) and to assess their potential tolerance to fungicides. Out of thirty PSB, two strains Klebsiella oxytoca and Enterobacter ludwigii were selected on the basis of their tolerance to fungicides. Both strains were assessed for their phosphate solubilizing ability using three different fungicides (difenoconazole, fluazinam and streptomycin) each with the concentrations of 0, 1, 2 or 3 times of the recommended rate. Both strains showed increased phosphate solubilization with difenoconazole at 1, 2 and 3 times of the recommended rate as compared to the phosphate solubilization of the control. The phosphate solubilization in Klebsiella oxytoca was recorded as 326, 538, 518 and 481 ${\mu}g\;mL^{-1}$ at 0, 1, 2 and 3 times of the recommended rate respectively, whereas in Enterobacter ludwigii it was recorded as 395, 499, 529 and 533 ${\mu}g\;mL^{-1}$ respectively at various doses. Based on the present findings, it may be concluded that both strains have the potential to be used as bio-inoculants which can solubilize phosphate even at the higher doses as compared to the recommended rate of fungicides.

• Korean Journal of Environmental Agriculture
• /
• v.29 no.2
• /
• pp.176-183
• /
• 2010

Endosulfan degrading ability of Klebsiella oxytoca KE-8 immobilized by entrapment with activated carbon was examined. Endosulfan degradation by the immobilized bacterial strains on several different activated carbon based support materials was investigated. Based on results, activated carbon ($8\times30$ mesh) was chosen as a support material. The immobilized Klebsiella oxytoca KE-8 with the cell density of 4 mg $g^{-1}$ (dry weight) degraded 22.18 ug $ml^{-1}$ endosulfan within 5 days at pH 7.0, $30^{\circ}C$ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug $ml^{-1}$ per day. Moreover, the endosulfan degradation activity could be demonstrated at $4^{\circ}C$ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.7 no.6
• /
• pp.347-351
• /
• 2002

Benzoate produced from the degradative pathways of various aromatic chemicals is generally recognized as a pollutant compound. However, various bacterial strains isolated as benzoate degraders have exhibited certain limits to their functions, including a loss of viability and degradability when cultivated in a broth medium for a longer time. Accordingly, immobilization techniques have been utilized to overcome such problems, and the current study examined the use of alginate and polyurethane for immobilizing Klebsiella oxytoca C302 to extend its viability and degradability of benzoate. The organism was well encapsulated by both matrices and the immobilized cells showed a high stability as regards their viability and degradability of 2 mM benzoate in a MM2 broth medium during cultivation for longer than 60 h in a semicontinuous batch system.

• Proceedings of the Korean Society of Life Science Conference
• /
• 2002.03a
• /
• pp.43-43
• /
• 2002