• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.545-551
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• 1994

Ethanol extract of barks, roots, stems, leaves and seeds of 49 species of plants were examined their growth inhibition by disk method on Listeria monocytogenes ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313. The extracts exhibited comparatively strong growth inhibition were compared their activity with broth culture containing different concentration of the extracts. L. monocytogenes ATCC 19111 was inhibited by Siegesbeckia pubescens Makino and Morus alba Linne (Ma) extracts. L. monocytogenes ATCC 19112 and ATCC 19114 were inhibited by Ma extract. ATCC 19113 was inhibited by Sophora flavescens AITON and Ma extract. ATCC 15313 was inhibited by Foeniculum vulgare Gaertner, Prunella vulgaris Var lilacina Nakai and Ma extract. Ma extract showed comparatively good inhibition effect for 5 strains of L. monoytogenes. 500 ppm of Ma extract almost inhibited the growth of all L. monocytogenes tested.

• Food Engineering Progress
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• v.15 no.4
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• pp.370-375
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• 2011

In this study the polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens in fresh originally grown vegetables. A universal primer (341GCf/534r) was selected for its ability to amplify the V3 region of 16S-rRNA genes in their target pathogens (Salmonella typhimurium, Pseudomonas fluorescens, Bacillus cereus, Listeria monoytogenes, Staphyloocus aureus, E. coli). The 194 bp fragments in PCR were successfully duplicated as expected. The amplified fragments of the same size from six different pathogens also showed good separation upon DGGE. The detection limit of PCR-DGGE for six pathogens in fresh-cut lettuces were over $10^{5}$ CFU/g when sampled by stomaching. However, when the sampling method was changed from stomaching to shaking, the detection limit of six pathogens in organic vegetables was shown to increase by over $10^{1}$ CFU/g, but only those of B. cereus were over $10^{3}$ CFU/g. Therefore, PCR-DGGE was shown to be a reliable method for the detection of pathogens in fresh-cut vegetables.