• Title/Summary/Keyword: LAM

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Isolation and Identification of Bacillus sp. LAM 97-44 Producing Antifungal Antibiotics (항진균성 항생물질을 생산하는 Bacillus sp. LAM 97-44의 분리 및 동정)

  • Lee, No-Woon;Kim, Cheon-Suk;Do, Jae-Ho;Jung, In-Chan;Lee, Hyean-Woo;Yi, Dong-Heui
    • Applied Biological Chemistry
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    • v.41 no.3
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    • pp.208-212
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    • 1998
  • In order to develop an effective antifungal antibiotics, over 700 isolates of bacteria, mold and actinomytes were screened from soil, and LAM 97-44 were selected as a strain producing the strong antifungal antibiotics against Candida albicans. Morphological, cultural and physiological characteristics of LAM 97-44 were investigated for the indentification. The cell size of LAM 97-44 was $2{\sim}3{\times}1{\sim}1.5\;{\mu}m$, and the shape of spore was of ellipsoidal. As a carbon source, LAM 97-44 utilized fructose, glucose, glycerol, maltose and raffinose but did not utilize arabinose, cellulose and xylose. The fatty acids of the cells included various iso-type and anteiso-type. Conclusively, the strain LAM 97-44 was proved to be Bacillus subtilis.

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Effect of Gleditsia sinensis Lam. Extract on Physico-Chemical Properties of Emulsion-Type Pork Sausages

  • Jin, Sang-Keun;Yang, Han-Sul;Choi, Jung-Seok
    • Food Science of Animal Resources
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    • v.37 no.2
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    • pp.274-287
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    • 2017
  • This study was performed to investigate the effect of Gleditsia sinensis Lam. extract on the physicochemical properties of emulsion-type pork sausages during storage at $10^{\circ}C$ for 4 wk. Treatments were as follows: (C, control; T1, sodium ascorbate 0.05%; T2, Gleditsia sinensis Lam. 0.05%; T3, Gleditsia sinensis Lam. 0.1%; T4, Gleditsia sinensis Lam. 0.2%; T5, Gleditsia sinensis Lam. 0.1% + sodium ascorbate 0.05%). The values of pH, moisture content, lightness, redness, and sensory attributes were all significantly decreased, while the yellowness, chroma, hue angle, and texture properties were increased during storage with increase of the Gleditsia sinensis Lam. extract added. In addition, the antioxidant activity and antimicrobial activity in the sausages displayed significant increases (p<0.05). Therefore, although it was concluded that the addition of Gleditsia sinensis Lam. extract is not effective for improvement of the physical properties compared to chemical additives in sausages, it could be applied to meat products as a natural preservatives.

A Study on the Block Shear Strength according to the Layer Composition of and Adhesive Type of Ply-Lam CLT (Ply-Lam CLT의 층재 구성 및 접착제 종류에 따른 블록전단강도에 관한 연구)

  • CHOI, Gyu Woong;YANG, Seung Min;LEE, Hyun Jae;KIM, Jun Ho;CHOI, Kwang Hyeon;KANG, Seog Goo
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.6
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    • pp.791-806
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    • 2020
  • In this study, a block shear strength test was conducted to compare and analyze the strength and failure mode on the glued laminated timber, CLT, and Ply-lam CLT, which are mainly used for the construction of wood construction as engineering wood. Through this, the Ply-lam CLT manufacturing conditions for optimum production, such as the type of lamina, plywood, adhesive, and layer composition, were investigated. The results are as follow. Through block shear strength test, it showed high strength in the order of glued laminated timber, Ply-lam CLT and CLT. In particular, the shear strength of Ply-lam CLT, which is made of a composite structure of larch plywood and larch lamina, passed 7.1 N/㎟, which is a Korean industrial standards for block shear strength of structural glued laminated timber. In addition, in this study, there was no different in shear strength according to the adhesive type used for glulam, CLT, and Ply-lam CLT adhesion. However, in the case of Ply-lam CLT, the difference in shear strength of Ply-lam CLT was shown according to the type of lamina and plywood. The results showed high strength in the order of Larix kaempferi > Mixed light hardwood ≒ Pinus densiflora, sieb, et, Zucc plywood. The optimal configuration of Ply-lam CLT is when larch plywood and larch lamina are used, and it is decided that the adhesive can be used by selecting PRF and PUR according to the application. The results of block shear strength failure mode by type of wood based materials were analyzed. The failure mode showed shear parallel-to-grain for glulam, rolling shear for CLT, and shear parallel-to-grain and rolling for ply-lam CLT. This is closely related to shear strength results and is decided to indicate higher shear strength in Ply-lam CLT than in CLT due to rolling shear.

Safety and Antioxydative effects of Cuscuta chinensis Lam. in PC12 Cell (PC12 Cell에 대한 토사자(?絲子)의 안정성 및 항산화작용에 대한 연구)

  • Do, Yong-Ho;Kim, Dong-Il
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.3
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    • pp.121-134
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    • 2006
  • Purpose : Cuscuta chinensis Lam. is utilized extensively as important medicines for threatened abortion habitual abortion. However, objective data related to an embryo is not existed until now. Therefore, this study is focused to find out stability of Cuscuta chinensis Lam. about an embryo during pregnancy based on data related to stability of nerve cell and antioxydative effect by using neural cell line, PC 12 cell. Methods : Experimentation concerns cytotoxic effects and antioxydative effect through methods such as MTT ssay, western botting after abstracting an undiluted solution from domestic Cuscuta chinensis Lam. Results : 1. As a result of experimentation on MTT assay according to each magnification from Cuscuta chinensis Lam. extraction solution with different abstraction methods, cytotoxic effect is not observed to all extract except an undiluted solution which is abstracted from MeOH stiring. Also, an undiluted solution in stiring with MeOH could not confirm whether come from Cuscuta chinensis Lam. or not. 2. As a result of revelation of Bax and GSK-3${\beta}$ which is responsive to the first stage from general stress in order to observe antioxydative effects of Cuscuta chinensis Lam. revelation of Bax by Cuscuta chinensis Lam. appeared to decrease. Conclusion : Cytotoxic effects with Cuscuta chinensis Lam. about PC12 cell is not discovered and it assume that it would be anti apoptotic effect by ROS as Bax and GSK-3${\beta}$ inviable effect. In the future, this study could be used as basic data for additional research on Cuscuta chinensis Lam. and effect and stability of complicated prescriptions for keeping pregnancy.

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A Design and Implementation of Communication Mechanism between User Contents and LAMS (사용자 콘텐츠와 LAMS 간의 통신 메커니즘의 설계 및 구현)

  • Park, Chan;Seong, Dong-Ook;Jung, Seok-In;Yoo, Jae-Soo;Yoo, Kwan-Hee
    • Proceedings of the Korea Information Processing Society Conference
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    • 2008.05a
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    • pp.764-767
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    • 2008
  • 컴퓨터와 인터넷이 발달과 함께 웹을 이용하여 다양한 학습 방법들이 개발되고 있다. 학습 활동 관리 시스템 LAMS는 효과적으로 학습 활동을 설계하고 관리할 수 있는 유용한 도구 중 하나이다. 하지만 LAMS가 우리가 플래시나 자바와 같은 외부 도구로 만든 외부의 사용자 콘텐츠들과 아무런 통신이 이루어지지 않기 때문에 완벽하게 학습 활동을 관리하지는 못한다. 본 논문의 목적은 이러한 문제를 해결하기 위해 LAMS와 외부의 사용자 콘텐츠가 서로 통신을 할 수 있는 통신 메커니즘을 설계하고 구현하는 것이다. 이를 위해 웹 기반 교육과 LAMS의 구조와 기능 및 개발 환경에 대해 분석 하였다. 이러한 분석을 기반으로 LAMS와 외부 교육용 콘텐츠 사이의 통신 메커니즘을 설계하고 구현하였다. 이러한 메커니즘을 플래시나 자바 또는 비주얼 C++로 만든 콘텐츠에 적용할 수 있으며, 이 콘텐츠들은 LAMS의 정보를 가지고 오거나 LAMS에 정보를 전달할 수 있다. 그리고 이러한 정보를 모아 각종 통계 자료를 만들고, 이를 교육에 반영할 수 있다. 이 메커니즘은 다양한 개발 환경에서 동작하며, 이것을 이용하여 기존의 LAMS에서는 불가능했던 다양한 학습 관리가 가능하도록 하였다. 우리는 이메커니즘을 이용하여 좀 더 혁신적인 콘텐츠를 개발하고 우수한 학습 활동 관리 시스템을 구축할 수 있을 것이다.

Purification and Characterization of an Antifungal Antibiotic from Bacillus subtilis LAM 97-44 (Bacillus subtilis LAM 97-44가 생산하는 항진균성 항생물질의 정제 및 특성)

  • Lee, No-Woon;Kwon, Tae-Jong;Yi, Dong-Heui
    • Applied Biological Chemistry
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    • v.46 no.2
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    • pp.69-73
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    • 2003
  • A novel antifungal antibiotic for azole-resistant Candida albicans was purified from the culture broth of Bacillus subtilis LAM 97-44 by butanol extraction, Diaion HP-20 and Dowex-50 adsorption chromatography, silica gel flash chromatography followed by HPLC and designated LAM-44A. LAM-44A was stable for 60 min at $100^{\circ}C$, and pH range from 2 to 10. MIC values were observed at $0.5-3.5\;{\mu}g/ml$ against various Candida albicans strains. The antibiotic showed no cytotoxicity for S180, MKN-45, P388, HeLa and 373 at the concentration of 1 mg/ml. LAM-f4A was colorless powder soluble in water, methanol, ethanol, butanol and negative to ninhydrin reaction. The antibiotic had maximum absorption at 273 nm in methanol, and melting point was $202^{\circ}C$. The molecular weight and formula were determined to be 282 and $C_{14}H_{34}O_5$ by $^1H-NMR,\;^{13}C-NMR$, IR spectrum and elemental analysis.

IFN-${\gamma}$mRNA Expression in Tuberculous Pleural Lymphocytes After in vitro Stimulation with M. tuberculosis Antigens (결핵균 항원 자극에 의한 결핵성 흉수 림프구의 IFN-${\gamma}$ mRNA 발현)

  • Park, Jae Seuk;Kim, Youn Seup;Jee, Young Koo;Lee, Kye Young
    • Tuberculosis and Respiratory Diseases
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    • v.57 no.1
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    • pp.25-31
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    • 2004
  • Background : IFN-${\gamma}$ is the main effector mediator of the host immune response against Mycobacterium tuberculosis. Evaluating the IFN-${\gamma}$ gene expression in response to M. tuberculosis antigens may help in elucidating the host defense mechanism against M. tuberculosis and in the development of a vaccine. Methods : The IFN-${\gamma}$ mRNA expression in the lymphocytes obtained from pleural effusions from tuberculous pleurisy patients (TB-PLC) after in vitro stimulation with whole cell M. tuberculosis(H37Rv), purified protein derivatives(PPD), man-lipoarabinamman (man-LAM), ara-LAM and Antigen 85B(Ag85B) were evaluated. The degree of IFN-${\gamma}$ mRNA expression was determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results : M. tuberculosis induced the expression of IFN-${\gamma}$ mRNA in the TB-PLC in time and dose dependent manners. The PPD and Ag85B induced high levels of IFN-${\gamma}$ mRNA expression in the TB-PLC. However, man-LAM inhibited IFN-${\gamma}$ mRNA expression in the TB-PLC, while ara-LAM did not. Conclusion : IFN-${\gamma}$ mRNA expression in TB-PLC is stimulated by PPD and Ag85B, but inhibited by man-LAM.

Prefeasibility Study on the Construction and the Operation of the Underground Cold Storage in Lam Dong Province, Vietnam (베트남 렁동성 지하냉장창고 건설 및 운영에 관한 예비타당성 조사)

  • Kim, Ho Yeong;Shin, Yong Hoon;An, Ji Hyun;Vinh, Bui Trong;Dung, Ta Quoc
    • Tunnel and Underground Space
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    • v.31 no.3
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    • pp.184-197
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    • 2021
  • The agricultural industry in Lam Dong Province has been growing in volume and kinds of produces. Various vegetables, fruits, flowers and coffee are the characteristics of the agriculture industry in Lam Dong. This situation makes the necessity of chilled and cold storages with commercial capacity. Another factor in Lam Dong is that there are more than 70 quarries under operation or abandoned. Upon the MOU between Lam Dong Government, BCMP and RIGPE, BCMP and RIGPE carried out the prefeasibility study on the construction of the underground cold storages using abandoned quarries in the Lam Dong area. They submitted the final report of the prefeasibility to the Lam Dong Government in August, 2018. In this report, the overall summary of the prefeasibility of the underground cold storage in Lam Dong is presented.

Imagining the Countryside in Literatures of the Eastern Lands: Juxtaposing "Dưới bóng hoàng lan" ("In the Ylang-Ylang Shade," 1942) by Thạch Lam (Thach Lam, Vietnam) and "Антоновские яблоки" ("Antonov Apples", 1900) by Иван Бунин (Ivan Bunin, Russia)

  • Do, Thi Huong
    • SUVANNABHUMI
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    • v.14 no.1
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    • pp.89-108
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    • 2022
  • Using Peter Barry's conception of "outdoor environment" in discoursing nature and culture, this article analyzes images of the countryside in the short stories "Dưới bóng hoàng lan" ("In the Ylang-Ylang Shade") by Thạch Lam (Thach Lam) and "Антоновские яблоки" ("Antonov Apples") by Иван Бунин (Ivan Bunin). The two share portray the Eastern Lands, as may be seen in Vietnamese northern countryside and the East Slavic, Byzantine. The paper focuses on three aspects of the countryside-cultural values; traces of urban life and; the aspirations of people. The article aims to emphasize people's desire to return to a type of nature that bears traces and harmonizes with human cultures.

A Study on the Analysis of Optimal Working Condition for Constant Temperature Laser MCT(LAM) Combined Machining (항온 Laser MCT(LAM) 복합 가공의 최적 가공 조건 해석)

  • Jeong-Ho Park;Gwi-Nam Kim
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.6_3
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    • pp.1197-1204
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    • 2023
  • Ti-alloy, a high-strength alloy material among the materials used in aircraft that are trending toward lighter weight, is classified as a difficult-to-cut material that requires a lot of energy for cutting. Cutting in a high-temperature environment is considered one means of making this possible, and various studies have been conducted on it. In particular, research on LAM (Laser Assisted Machining (LAM)), which utilizes laser heating of the cutting area, is being actively conducted. Before processing of the milling cutter begins, the temperature is raised locally by the laser irradiated through the laser head carrier, and the resistance during milling is reduced. Therefore, in this paper, in order to derive such conditions, we performed heat transfer analysis according to transfer conditions and compared it with actually applied test data to use it to establish appropriate processing conditions.