• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.167-172
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• 2007

A tomato zinc-finger protein gene, LeZFP1, encoding the Cys2/His2-type zinc-finger transcription factor was searched from cDNA microarray analysis of gene expression following induction of the overexpressed tomato transgenic plants showing resistance for pathogen and abiotic stresses. The full-length cDNA of LeZFP1 encoded a protein of 261 amino acid residues. Analysis of the deduced amino acid sequence of LeZFP1 revealed that it shares high sequence identity with pepper CAZFP1 (81% identity). We found that single copy of LeZFP1 gene is present in the tomato genome through southern blot analysis. The LeZFP1 transcripts were constitutively expressed in the tomato mature and young leaves, but were detectable weakly in the flower, stem and root. The LeZFP1 transcripts were significantly reduced in treated leaf tissues with NaCl and mannitol. The LeZFP1 gene was induced by oxidative stress especially. Our results indicated that LeZFP1 may play a role function involved in oxidative stress signaling pathways.

• BMB Reports
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• v.42 no.9
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• pp.611-616
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• 2009

We investigated the expression pattern of dehydration responsive element-binding protein-3 in tomato under different abiotic stresses. Full length LeDREB3 cDNA was isolated from tomato plant, followed by phylogenetic analysis based on deduced amino acid sequences that revealed significant sequence similarity to DREB proteins belonging to diverse families of plant species. Southern blot analysis showed duplicate copies of LeDREB3 in the tomato genome while organ-specific expression profiling indicated constitutive expression of LeDREB3 in all tested organs, which was particularly strong in flower. LeDREB3 expression was significantly induced by Nacl, drought, low temperature and $H_2O_2$. Moreover, LeDREB3 was slightly regulated by treatment with ABA and MV. These observations suggest that the LeDREB3 gene may be involved in the response of the tomato plant to stress.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.192-197
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• 2013

This study examined the phenotypic and molecular characteristics of the $2^{nd}$ clone ($T_0V_2$) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the npt II gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.

• Journal of The Korean Digital Architecture Interior Association
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• v.7 no.2
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• pp.33-41
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• 2007

This thesis aims at understanding Le Corbusier's architecture through study of proportion. Based on the analysis of these texts, four elements - geometry in the outline, diagonal regulation lines in the facade, arithmetic rhythm in the structure, composition in the inner space - were listed. Through these four proportional systems. ten houses of 1920's, designed by Le Corbusier were analysed. The Le Corbusier's proportional system can be classified by two different purposes ; aesthetics and utility standardization for the mass production was reflected. In the later period, the proportion system of 1920's has changed through the process of self-contradiction. But the concept of the golden section and the human scale was reflected on Modulor, which Le Corbusier created in 1940's. Therefore, Le Corbuiser's notion of proportion for harmony of architecture has consistent meaning throughout all of his works from the 1920's.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.64-68
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• 2014

Up to date several mycoviruses including Lentinula edodes Spherical Virus (LeSV) have been reported. As fungal virus was spreaded by infested hypae and spores it could be important to use virus-free spawns to eradicate the mushroom virus disease in the culture farm. We tested the imported spawns of Lentinula edodes by PCR whether LeSV was infested them or not. The primer set targeting the RdRp gene of LeSV was prepared based on partial sequence of the LeSV genome. The RT-PCR analysis showed that 87 among 88 imported spawns of L. edodes were infested by LeSV.

• Korean Journal of Applied Biomechanics
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• v.22 no.3
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• pp.341-348
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• 2012

The purpose of this study was to compare the muscle activities of the thoracic extensor(TE) and lumbar extensor(LE) during trunk lift (TL) exercise according to exercise position. Seventeen healthy subjects with no medial history of back pain were recruited for this study. Subjects performed the TL exercise in prone, quadruped and heel-sitting positions. The activities of the TE and LE were measured using surface electromyography during TL exercise in each exercise position. A one-way repeated-measures analysis of variance (ANOVA) was used to compare the normalized muscle activities of the TE and LE and the TE/LE ratio. The results showed that there was not significant effect of exercise position on the muscle activities of TE(p>.05). However, there was significant effect of exercise position on the muscle activities of LE and the TE/LE ratio(p>.05). Post hoc pair-wise comparisons with Bonferroni correction showed that both muscle activities of LE and TE/LE ratio in prone position were significantly different in those in heel-sitting and quadruped positions, during TL exercise, respectively. The TE/LE ratio was the greatest for TL exercise in heel-sitting position. Therefore, for selective activation of the TE muscle, we recommend performing the TL exercise in heel-sitting or quadruped position.

• Journal of architectural history
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• v.13 no.2 s.38
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• pp.7-19
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• 2004

This study is an attempt to prove a relationship between Le Corbusier's utopian attitude towards nature and architectural ideology and ideology by a form of inquiry into sociological interests. That is to find out contradictions and limitations in Le Corbusier's architectural world, namely particularity of his ideology by exploring the base entangled with special interests in the society up until formation of his architectural language. A process of this study is by; 1. Examining of background which affected Le Corbusier's attitude towards nature until the nature became his architectural language; 2. An analysis of Le Corbusier's architectural works. That is, this study's main interest is on formation of Le Corbusier's architectural language by his attitude towards nature which present in residential field especially focused on Villa Meyer and Villa Savoye, two best examples of his villa. 3. This study have a goal to investigate that utopian and ideological conception in architecture of Le Corbusier have a dialectic relationship between them. This goal can be achieved by observing in what ways Le Corbusier's utopian attitude towards nature is expressed in residential architectural language and researching into his inconsistent architectural ideology derived from applying architectural language present in villa to pre-fabricated apartment houses.

• Biomedical Science Letters
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• v.28 no.1
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• pp.34-41
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• 2022

In this study, conditions for extraction of functional component from Nypa fruticans wurmb was optimized. The yield by extraction with 30% ethanol (LE30, 42.12%) was higher than those hot water extraction (LDW 33.32%), 50% ethanol (LE50, 40.12%) and 70% ethanol (LE70, 34.5%). The extract was purified and analyzed by GC MS. The prevailing compounds found in extract were Cyclodecasiloxane-, pentadecanoic acid, -eicosane, undecanal and tridecanoic acid. The presence of saturated and unsaturated fatty acids in ethanolic extract vindicate the use of this plant to treat many diseases in traditional medicine. The total phenolic contents in the LDW, LE30, LE50, LE70 extract were 128±1.65 mg/g, 205±2.3 mg/g, 210±4.23 mg/g and 180±5.6 mg/g, respectively. The DPPH was highest in LE70 extract (1,000 ㎍/mL, 81.14%), ABTS was highest in LE50 extract (1,000 ㎍/mL, 84.14%). The protective effects against oxidative stress in raw 264.7 cell imparted by the LE50 extract was better than those imparted by the other extracts. The findings of the present study suggest that 50% ethanol is best solvent for extraction of Nypa fruticans Wurmb, considering yield, polyphenol content, and antioxidant activities with extraction cost.

• Korean Journal of Environmental Biology
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• v.23 no.3 s.59
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• pp.215-220
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• 2005

Through multiple stage analysis, the biochores in Korea were classified and organized as a hierarchy system. In the 1st step, the isopleth lines of warmth index $100^{\circ}C,\;85^{\circ}C,\;55^{\circ}C\;\cdot$ month and the coldness index $-8^{\circ}C,\;or-10^{\circ}C\;\cdot$ month, which indicate the boundaries of plant formation zones (Yim and Kira 1975; Yim 1977), were applied in the determination of major biochores. In the 2nd step, these biochores were subdivided into the five classes based on Thornthwaite's moisture index (Im) and Yim and Kira (1976), as follows: $100{\le}Im,100 In the 3 rd step, the analysis of topographic features yielded three categories of flatlands, gentle slope, and steep slope areas. These were obtained by adopting the $100{\times}100-meter$ gridded DEM and by considering the physical features of the Korean Peninsula. The features of relief in mountainous areas, waters, islands, etc. were converted into climatic indices. This grouping of biochores serves as a useful tool for the interpretation of the distributional patterns of vegetation of vascular plants and similar phenomena.

• Journal of the Korean Society of Systems Engineering
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• v.4 no.1
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• pp.35-43
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• 2008

Generally, in analysis of reliability of Design and Development Phase, reliability of electrical components is analyzed based on standards such as MIL-HDBK-217F, Bellcore Issue 4,5,6 by analyzing stress of architectural side (Power, Voltage, Current and quality level of components) of weapon system and stress of operational side (operational environment, operational temperature, Operational Profile). But the reliability of mechanical components is analyzed based on the data book of failure history of mechanical parts called NPRD-95(Nonelectronic Parts Reliability Data-95) without any analysis of above stress. However, even if it's the same mechanical parts, it might have different failure rate(fatigue, wear, corrosion) during operation depending on how weary(stress : pressure, vibration, temperature during operation) the parts are. Therefore, analyzing reliability using just data book can cause big difference in reliability instead of analyzing based upon stressfulness that parts might have, operational concept, and other various factors. Thus, This paper will guide the way of predicting reliability by organizing ways of predicting reliability for system organization and adopt ing NSWC-98/LE1(Naval Surface Warfare Center-98/LE1) for mechanical components.