• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1402-1405
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• 2003

The QTL(quantitative traits loci) linkage mapping of Hanwoo (Korean Cattle) chromosome 6 for daily gain and marbling score was performed using 378 individuals from 18 paternal half-sib families in Hanwoo. Hanwoo chromosome 6 were mapped to total length of 394.2 cM between 28 microsatellite loci using 36 microsatellite primers of BTA 6 linkage group. The QTL analysis for daily gain in Hanwoo showed 8 microsatellite loci (BM3026-5.66, EL03-5.58, BM4311-5.29, ILSTS035-4.50, BMS1242-4.37, BM1329-3.67, BM415-3.11, BMS2460-3.03) in larger than LOD score 3.0. Based on the QTL analysis for marbling score, LOD scores of 12 microsatellite loci (BM415-8.88, BM3026-7.15, ILSTS093-5.45, ILSTS035-4.91, EL03-4.69, BMS690-4.52, BM1329-4.43, BMS511-3.74, BMS1242-3.66, BMS518-3.65, BM4311-3.41, BMC4203-3.36) were found larger than 3.0.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.6
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• pp.565-570
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• 2006

The viviparous germination (VG) with lodging caused the yield reduction and quality deterioration in rice. We carried out the evaluation of VG tolerance (on the 40th day after heading) and mapping QTLs associated with VG tolerance using the recombinant inbred lines (M/G RILs) from a cross between Milyang 23 (japonica/indica) and Gihobyeo (japonica). The VG rates of Milyang 23 and Gihobyeo were 0.0 and 7.0%, respectively. The averaged VG rate of 162 M/G RILs was 7.7%, and their range was from 0.0 to 50.9%. Of the 162 RILs, 144 lines were tolerant less than 10%, and 18 lines were susceptible more than 10%. Using the M/G RIL Map, three QTLs associated with the viviparous trait were detected on chromosome 2 (qVG 2-1 and qVG 2-2) and 8 (qVG 8). qVG 2-1 was linked to RM 32D and RZ 166, and had LOD score of 2.97. qVG 2-2 was tightly linked to E13M59.119-Pl and E13M59.M003-P2, and showed higher LOD score of 3.41. qVG 8 was linked to RM33 and TCT116, and had LOD score of 2.67. The total phenotypic variance explained by the three QTLs was about 24.4% of the total variance in the population. The detection of new QTLs associated with VG tolerance will provide important informations for the seed dormancy, low temperature germination, or comparative genetics.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.279-279
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• 2022

Rice is 34% of the world's population used as a staple food. But the world population is increasing. Food security is not well protected. Improving cultivar development can address food security. Quantitative trait locus (QTL) mapping is a statistical analysis using both phenotypic and genotypic dates. The purpose of QTL mapping is to determine a gene. Increasing grain size is a way to increase yield in rice. Grain size-related genes were mapped using CNDH population obtained by cross-breeding Cheongcheong (Indica) and Nagdong (Japonica) through anther culture. Grain harvested from experimental field of Kyungpook National University in Gunwi in 2021. Genes related to grain length were detected between RM5964-RM12285, RM20924-RM20967 in chromosome 1, 7. LOD score is 5.88 and 5.6. Genes related to grain width was detected between RM289-RM18130 in chromosome 5. LOD score is 4.57. Genes related to grain length/width ratio were detected between RM5459-RM3482, RM5699-RM1211 and RM3838-RM3381 in chromosome 1, 2, 5. LOD score is 3.75, 3.14 and 3.41. 4 genes was detected in chromosome 1 and 2 genes was detected in chromosome 2 and 7 genes was detected in chromosome 5. 2 genes related to grain shape and quality were detected. 4 genes related to grain length were detected. 4 genes related to grain size were detected. 1 gene related to grain size and weight was detected. 2 genes related to grain length and weight were detected. By finding the gene related to grain size, it provides food to people threatened by food security and solves the food shortage.

• Journal of Life Science
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• v.11 no.3
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• pp.279-283
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• 2001

Amyotrophic lateral sclerosis(ALS) is a progressive neurologic disorder resulting from the degeneration of upper and lower motor neurons, and is inherited in 10% of cases. About 20% of familial ALS, clinically indistinguishable from sporadic ALS, is caused by mutations of Cu/Zn superoxide dismutase on chromosome 21q22.21 inherited as an autosomal dominant trait. We now report a new locus in the non-SOD1 dominantly inherited ALS. We screened a large ALS family with 11 affected individuals and one obligate gene carrier with genome-wide ABI polymorphic markers using the ABI 377 automated system. No evidence of linkage was obtained with the autosomal markers. We next screened this family with X chromosome markers as there was no evidence of male-to-male tran-smission of the disease. Linkage was established with several X chromosome markers with a lod score up to 3.8; almost the maximum possible score in this family. Our finding imply that a gene for the dominant expression of a neuronal degeneration is coded on X chromosome and raise the question of the role of X-linked genes that escape inactivation in this pathogenesis. More importantly, our finding that a gene causing ALS is localized on X-chromosome has direct investigational relevance to sporadic ALS, where epidemiological studies show male gender predominance(1.3:1) and earlier onset in men by 5-10 years.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1033-1038
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• 2004

LOD scores related to marbling scores and permutation test have been applied for the purpose detecting quantitative trait loci (QTL) and we selected a considerable major locus BM4311. K-means clustering, for the major DNA marker mining of BM4311 microsatellite loci in Hanwoo chromosome 6, has been tried and five traits are divided by three cluster groups. Then, the three cluster groups are classified according to six DNA markers. Finally, bootstrap test method to calculate confidence intervals, using resampling method, has been adapted in order to find major DNA markers. It could be concluded that the major markers of BM4311 locus in Hanwoo chromosome 6 were DNA marker 100 and 95 bp.

• Journal of the Korean Data and Information Science Society
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• v.18 no.1
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• pp.175-184
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• 2007

LOD scores and a permutation test for detecting and locating quantitative trait loci (QTL) from the Hanwoo economic trait have been described and we selected a considerable major BMS1167 locus for further analysis. K-means clustering analysis, for the major DNA marker mining of BMS1167 microsatellite loci in Hanwoo chromosome17, has been tried and three cluster groups divide four traits. The three cluster groups are classified according to eight DNA marker bps. Finally, we employed the bootstrap test method to calculate confidence intervals using the resampling method to find major DNA markers. We conclude that the major marker of BMS1167 locus in Hanwoo chromosome17 is only DNA marker 100bp.

• Journal of the Korean Data and Information Science Society
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• v.18 no.4
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• pp.1103-1113
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• 2007

LOD scores and a permutation test for detecting and locating Quantitative trait loci(QTL) from the Hanwoo economic trait have been described and we selected a considerable major BMS941 locus. K -means clustering analysis of eight markers in BMS941 and four traits resulted in three cluster groups. Finally, we applied the bootstrap test method to calculate confidence intervals for finding major DNA markers. We conclude that the major markers of BMS941 locus in Hanwoo chromosome 17 are markers 85bp and 105bp.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.91-91
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• 2017

Drought is the most serious abiotic stress limiting rice production. However, little progress has been made in the genetic analysis of drought tolerance, because it is a complex trait controlled by a number of genes and affected by various environmental factors. In here, we screened 218 rice genetic resources for drought tolerance at vegetative stage and selected 32 highly drought tolerant varieties in greenhouse. Under rain-fed conditions, Grain yield of Nagdong was decreased by 53.3% from 517 kg/10a to 241 kg/10a when compare to irrigation condition. By comparison, grain yield of Samgang was decreased by 23.6% from 550 kg/10a to 420 kg/10a. The variety Samgang exhibited strong drought tolerance and stable yield in rain-fed conditions and was selected for further study. To identify QTLs for drought tolerance, we examined visual drought tolerance (VDT) and relative water content (RWC) using a doubled haploid (DH) population consisted of 101 lines derived from a cross between Samgang (a drought tolerance variety) and Nagdong (a drought sensitive variety). Three QTLs for VDT were located on chromosomes 2, 6, and 11, respectively, and explained 41.8% of the total phenotypic variance. qVDT2, flanked by markers RM324 and S2016, explained 8.8% of the phenotypic variance with LOD score of 3.3 and an additive effect of -0.6. qVDT6 was flanked by S6022 and S6023 and explained 12.7% of the phenotypic variance with LOD score of 5.0 and an additive effect of -0.7. qVDT11, flanked by markers RM26765 and RM287, explained 19.9% of the phenotypic variance with LOD score of 7.1 and an additive effect of -1.0. qRWC11 was the only QTL for RWC to be identified; it was in the same locus as qVDT11. qRWC11 explained 19.6% of the phenotypic variance, with a LOD score of 4.0 and an additive effect of 9.7. To determine QTL effects on drought tolerance in rain-fed paddy conditions, seven DH lines were selected according to the number of QTLs they contained. Of the drought tolerance associated QTLs, qVDT2 and qVDT6 did not affect tiller formation, but qVDT11increased tiller number. Tiller formation was most stable when qVDT2 and qVDT11 were combined. DH lines with both of these drought tolerance associated QTLs exhibited the most stable tiller formation. These results suggest that qVDT11 is important for drought tolerance and stable tiller formation under drought stress condition in field.

• Journal of Life Science
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• v.10 no.2
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• pp.12-13
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• 2000

A human-specific retroposon SINE-R.C2 has been derived from a human endogenous retrovirus HER V-K 10. It is absent in the genome of nonhuman primates and present within the third intron of the human C2 gene that is located in the class III region of the major histocompatibility complex. In the present study, we determined the regional location of the human C2 gene. The analysis of the Genebridge 4 radiation hybrid mapping panel using PCR amplification located the C2 gene between D6S1422 (10.1 cR) and CHLC.GATA4A03 (21.3) with a lod score of>3.0. This allowed us to localize C2 gene on the human chromosome 6 band p21.31.