• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.499-505
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• 1987

The functional properties of lupin seed protein concentrate (LPC) were examined and compared to those of soybean protein isolate (SPI) and Na-caseinate. LPC-50, of which protein level was 50%, was prepared by a two phase solvent (hexane: alcohol: water= 10:7:3) extraction method. LPC-70 was made from LPC-50 by removing the fractions solubilized by carbohydrate decomposing enzymes. The solubilities of LPC-50 and LPC-70 were similar to that of of SPI but slightly higher at pH 4-5, and less susceptible to the added salt. The apparent viscosity of LPC increased exponentially as the concentration increased over 6% level, and the change was similar to that of Na-caseinate. LPC showed strong pseudoplastic non-Newtonean flow behavior, which was similar to that of SPI The emulsifying capasity of LPC-70 was similar to that of SPI when salt was added. The foaming capacity of LPC was comparable to that of SPI. LPC showed high oil and water absorption capacities, which increased as the protein level was elevated. LPC-70 showed the highest oil absorption capacity of all the samples tested.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.3
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• pp.175-182
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• 2022

Translocation of azurophil granules is pivotal for bactericidal activity of neutrophils, the first-line defense cells against pathogens. Previously, we reported that lysophosphatidylcholine (LPC), an endogenous lipid, enhances bactericidal activity of human neutrophils via increasing translocation of azurophil granules. However, the precise mechanism of LPC-induced azurophil granule translocation was not fully understood. Treatment of neutrophil with LPC significantly increased CD63 (an azurophil granule marker) surface expression. Interestingly, cytochalasin B, an inhibitor of action polymerization, blocked LPC-induced CD63 surface expression. LPC increased F-actin polymerization. LPC-induced CD63 surface expression was inhibited by both a Rho specific inhibitor, Tat-C3 exoenzyme, and a Rho kinase (ROCK) inhibitor, Y27632 which also inhibited LPC-induced F-actin polymerization. LPC induced Rho-GTP activation. NSC23766, a Rac inhibitor, however, did not affect LPC-induced CD63 surface expression. Theses results suggest a novel regulatory mechanism for azurophil granule translocation where LPC induces translocation of azurophil granules via Rho/ROCK/F-actin polymerization pathway.

• Proceedings of the IEEK Conference
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• 2001.09a
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• pp.833-836
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• 2001

본 논문에서는 한국어 음성인식 시스템의 성능 향상을 위해 청각 주파수 분해능을 가진 MEL-LPC Cepstrum을 음소단위의 HMM(Hidden Markov Model)을 기반으로 하는 인식 시스템에 적용하여 그 결과를 비교 검토하였다. 선형예측(LP) 분석 후에 후처리로서 주파수를 왜곡시킨 LPC-MEL 분석이 계산량이 적고 효과적이라 일반적으로 많이 사용되고 있으나 주파수 분해능은 많이 개선되지 않는다. 따라서 본 논문에서는 주파수 분해능을 개선하기 위해, 원 음성신호로부터 직접적으로 멜주파수로 왜곡시킨 후 선형 예측 분석을 수행하는 MEL-LPC 분석방법을 이용한 음소기반의 화자 독립 음성인식 시스템을 구성하여 기존의 LPC-MEL 분석방법과 비교실험을 통하여 MEL-LPC 분석방법의 유효성을 검토하였다. 실험에 사용한 음성 데이터베이스는 음소 및 단어 인식실험에서는 ETRI 445단어 DB, 연속 숫자음인식 실험에서는 KLE 4연속 숫자음 DB를 사용하였다. 화자 독립 음소인식 실험의 경우, 묵음을 제외한 47개의 유사 음소에 대하여 4상태 3출력의 Left-to-Right 모델을이용하였다. 단어 및 연속 숫자음 인식 실험의 경우, 유한상태 네트워크에 의한 OPDP법을 이용하였다. 화자 독립 음소, 단어 및 4연속 숫자음 인식 실험결과, 기존의 LPC-MEL Cepstrum을 사용한 경우보다 MEL-LPC Cepstum을 사용한 경우가 더 높은 인식률을 나타내어 한국어 음성인식 시스템에서 MEL-LPC 분석방법의 유효성을 확인할 수 있었다.

• Analytical Science and Technology
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• v.5 no.3
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• pp.339-343
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• 1992

The content of lysophosphatidyl choline (LPC) in wheat starch lipids from six cultivar representing three classes of wheat was determined by a high performance liquid chromatography using UV-detection (HPLC-UV). The HPLC-UV assay had a sensitivity of LPC concentrations above $5{\mu}g/50{\mu}l$ and required 80 minutes per chromatogram.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.3
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• pp.259-263
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• 1983

This experiment was carried out to investigate the effect of pH at coagulation on fatty acid and sterol composition of green LPC. Green juice of Italian ryegrass, red clover, oats and alfalfa was adjusted at pH 4, 6, 8 and heated at $85^{\circ}C$ for ten minutes. Green LPC was taken from the above green juice. The results obtained were as follows. 1. Green LPC of Italian ryegrass, oats and alfalfa contained much on the order of linoleic acid, palmitic acid and linolenic acid. Methyl ester of these three fatty acid accounted for 70.4% to 86.2% of total fatty acid methyl ester. Green LPC of red clover contained much palmitic acid, linoleic acid, and linolenic acid. These three fatty acid methyl ester accounted for 82.5% to 86.2% of total fatty acid methyl ester. 2. Linolenic acid was highest in the green LPC of red clover, oats and alfalfa which was adjusted at pH 8 and heated and coagulated, and next at pH 6, and pH 4, respectively. Linolenic acid was highest in the green LPC of Italian ryegrass which was adjusted at pH 6, next pH 8, and pH 4. 3. Green LPC of red clover and alfalfa contained cholesterol. The all green LPC contained campesterol, stigmasterol and sitosterol. 4. Sterol was highest in the green LPC of Italian ryegrass, red clover and oats which was adjusted at pH 8 and next at pH 4, and pH 6, respectively. Sterol was highest in the green LPC of alfalfa which was adjusted at pH 8, and next at pH 6, and pH 4, respectively.

• Journal of KIISE:Databases
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• v.29 no.2
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• pp.128-137
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• 2002

In this paper, we propose the LPC+-file for efficient indexing of high-dimensional image data. With the proliferation of multimedia data, there Is an increasing need to support the indexing and retrieval of high-dimensional image data. Recently, the LPC-file (5) that based on vector approximation has been developed for indexing high-dimensional data. The LPC-file gives good performance especially when the dataset is uniformly distributed. However, compared with for the uniformly distributed dataset, its performance degrades when the dataset is clustered. We improve the performance of the LPC-file for the strongly clustered image dataset. The basic idea is to adaptively partition the data space to find subspaces with high-density clusters and to assign more bits to them than others to increase the discriminatory power of the approximation of vectors. The total number of bits used to represent vector approximations is rather less than that of the LPC-file since the partitioned cells in the LPC+-file share the bits. An empirical evaluation shows that the LPC+-file results in significant performance improvements for real image data sets which are strongly clustered.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.3
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• pp.251-258
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• 1983

This experiment was carried out to investigate the capability of production of artificial milk for leaf protein concentrate (LPC). Chloroplastic protein and cytoplasmic protein were extracted from leaves of Dystaenia takeshimana Nakai and LPC was extracted from leaves of Italian ryegrass to increase the functional properties of LPC as a level of milk casein. One gram of chloroplastic protein and cytoplasmic protein and 1g of LPC were succinylated by addition of succinic anhydride 0.1, 0.25, and 1g respectively. Their functional properties were investigated in this experiment. The results obtained were summarized as follows: 1. The non-succinylated LPC showed a higher value in bulk density than the chloroplastic protein, the cytoplasmic protein and LPC succinylated by addition of succinic anhydride 0.1, 0.25, and 1g respectively. Nevertheless, succinylation had an enhancing effect as indicated by the rises as the degrees of succinylation was increased. 2. Although solubility of non-succinylated LPC was lower than that of milk casein, succinylation caused an effective increase in the solubility of the protein and LPC. 3. Water absorption and fat absorption of succinylated LPC were twice to eight times higher than those of milk casein. Fat absorption was not influenced to the extent by succinylation as the water absorption. Excessive succinylation resulted in the decrease of both water absorption and fat absorption. 4. Emulsifying activity and emulsion stability were increased in proportion to the succinylated degree of LPC. More than 10% increase in the amount of succinic anhydride resulted in an apparent increase in emulsifying activity and emulsion stability. Besides, the succinylated LPC showed more excellent functional properties in emulsifying activity and emulsion stability than milk.

• Biomolecules & Therapeutics
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• v.16 no.2
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• pp.69-76
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• 2008

Lysophosphatidylcholine (LPC) is an endogenous phospholipid. LPC has various stimulating or modulating activities on immune cells, including lymphocytes, monocytes/macrophages and neutrophils. Studies generally revealed pro-inflammatory activities of LPC, but accumulating evidence indicates that LPC has also anti-inflammatory actions. Here we summarize immunomodulatory actions of LPC.

• Proceedings of the KIEE Conference
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• 2001.07d
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• pp.2516-2518
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• 2001

The quantization of the LPC parameters is a very important aspect of the speech compression algorithm. This paper analyzes the quantization effect of the LPC coefficients and presents the implementation of a fixed-point CELP coder based on the LPC analysis.

• Pediatric Infection and Vaccine
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• v.6 no.2
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• pp.253-260
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• 1999

Purpose : The purpose of this study is to make and use monoclonal Ab which reacts with CMV major immediate early(${\alpha}$) protein(p72). Methods : Normal human fibroblast(Foreskin derived) was cultured in Eagle's minimal essential medium(MEM) containing 10% cowfetus serum and mouse chondroblast was cultured in P3X63 Ag8.653(ATCC. Maryland USA) to maintain $5{\times}10^5/ml$ cell counts. CMV(KJHJ90) from congenital CMV infected infant's urine was multiplied and used for Ab making. CMV Ag was injected 4 times, 1 week interval into the peritoneal space of 6~8 weeks old mice. And then lymphocyles and fibroblasts taken from spleen were obtained and conjugated. After the conjugated cell cultured, we chose the cell that had high Ab titer using indirect immunofluerescent method. Results : Among the 28 monoclonal antibodies obtained LPC12 and LPC23 reacted highly with nucleus of AD169 infected cell. Purified AD169 after SDS-PAGE, molecular weight of Ag, which reacted with purified monoclonal Ab, was obtained using Western blotting. Monoclonal Ab of LPC12 and LPC23 clone reacted most highly with 72 kd Ag. Conclusion : LPC12 and LPC23 clonal Ab with AD 169(P63-27) is useful on early diagnosis of CMV infection.