• Title/Summary/Keyword: Leclercia Adecarboxylata

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Unexpected Isolation of Leclercia Adecarboxylata in Dermatitis of a Dog

  • Jung, Hansol;Cho, Hyunkee;Lee, June Bong;Yoon, Jang Won;Chung, Jin-Young
    • Journal of Veterinary Clinics
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    • v.34 no.5
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    • pp.381-383
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    • 2017
  • A two-year-old intact male Labrador retriever was presented with generalized erythema, pustule and pruritus. A skin screening test revealed that there were no fleas but bacteria and dermatophytes were present. Blood testing revealed no remarkable findings. The patient was prescribed systemic medication of enrofloxacin 30 mg/kg once a day and itraconazole 10 mg/kg once a day and topical medication of 2% chlorhexidine shampoo twice a week for 2 weeks. Two weeks after the prescription, aerobic culture confirmed that the bacteria were Leclercia Adecarboxylata and Pseudomonas putida was sensitive to enrofloxacin. Therefore, more medicine was prescribed for 4 weeks to alleviate clinical signs. After six weeks of medication, clinical signs were alleviated and skin screening test revealed no remarkable findings. Bacterial and fungal skin infections are common in dogs. However, there are no reports of Leclercia Adecarboxylata infection even in gastrointestinal tract in veterinary medicine. This is the first report of Leclercia Adecarboxylata infection in dogs. This report proved that Leclercia Adecarboxylata can cause skin problem in dogs.

Removal Characteristic of Ammonia Nitrogen and Behavior of Nitrogen in Synthetic Wastewater Using Leclercia Adecarboxylata (Leclercia Adecarboxylata를 이용한 합성폐수의 암모니아성질소 제거특성 및 질소거동)

  • Lee, Hyun-Hee;Phae, Chae-Gun
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.4
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    • pp.460-465
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    • 2007
  • In this study, the removal characteristic of ammonia nitrogen and behavior of nitrogen was investigated using Leclercia adecarboxylata, which was derived from the culture contaminated by ammonia nitrogen of high concentration. The method of ammonia nitrogen removal was not biological nitrification and denitrification but elimination of nutrient salt with internal synthesis of microorganisms which use ammonia nitrogen as substrate. L. adecarboxylata(one of ammonia synthesis microorganisms) was highly activated and showed the most high removal efficiency in free salt condition but the removal efficiency decreased badly in salt concentration of more than 4%. About 80 mg/L of $NH_3-N$ was mostly removed within 20 hours and 500 mg/L of $NH_3-N$ showed less then removal efficiency of 50% because carbon source was not enough. However, ammonium nitrogen concentration was decreased again when the carbon source was inserted additionally thus, ammonium nitrogen removal efficiency by L. adecarboxylata, was related to amount of carbon source. pH decreased from 8.0 to 6.36 according to growth of L. adecarboxylata. Concentration of nitrite nitrogen and nitrate nitrogen did not increase and TKN concentration showed no variation while ammonia nitrogen was removed by L. adecarboxylata. In addition to, when content of protein in organic nitrogen was measured, protein was not detected at the beginning of microorganism synthesis but protein of 193.1 mg/L was detected after 48 hours. Hence, ammonium nitrogen was not decomposed as nitrate nitrogen and nitrite nitrogen but synthesized by L. adecarboxylata, which has excellent ability of nitrogen synthesis and can threat ammonia nitrogen of high concentration in wastewater.

Isoprenoid Quinone Profiles of the Leclercia adecarboxylata KCTC $l036^T$

  • Shin, Yong Kook;Jung Sook Lee;Chang Ouk Chun;Hong Joong Kim;Yong Ha Park
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.68-69
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    • 1996
  • The isoprenoid quinone composition of Leclercia adecarboxylata KCTC $1036^T$ was determined by using high-performance liquid chromatography. L. adecarboxylata KCTC $1036^T$ are characterized by their production of both ubiquinone-7, ubiquinone-8 and menaquinone-8 as major quinones. It is clear that the analysis of isoprenoid quinone profiles provides a new criterion of great promise for identifying Leclercia strains.

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Cloning. Sequencing and Characterization of the Novel Penicillin G Acylase Gene from the Soil-isolated Leclercla adecarboxylata

  • Jun , Sang-O.;Lim, Ho-S.;Kim, Geun-Y.;Lee, Eung-S.;Lee, Mann-H.
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.331.3-332
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    • 2002
  • A novel penicillin G acylase (PGA)-producing bacterial strain was isolated from soil by using the Serratia marcescens overlay technique. The isolated strain was identified as Leclercia adecarboxylata based on the analyses of the biochemical characteristics (API 20E). the cellular fatty acid profile. and the 16S rDNA sequences. The gene encoding the PGA (pac gene) was cloned into the pHSG399 vector and the recombinant E. coliHB101 clones harboring the pac gene were isolated on agar plates containing phenylacetyl-L -leucine and penicillin G. (omitted)

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