• 미생물학회지
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• 제29권5호
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• pp.267-269
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• 1991

The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.

• 식물조직배양학회지
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• 제26권4호
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• pp.223-227
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• 1999

본 연구는 글라디올러스 캘러스로부터의 재분화 조건을 구명함으로써 캘러스 배양시스템을 확립하는데 기여하고자 하였다. 글라디올러스 캘러스로부터의 유식물체 획득은 캘러스를 2,4-D 무첨가 1/2 MS고체배지에 치상한 후 24시간 일장하에서 15$^{\circ}C$로 배양하였을 때 효과적이었다. 캘러스로부터의 기관형성에 미치는 배양방법의 영향을 검토한 결과, 부정근의 형성은 액체진탕배양이 그리고 부정아의 형성은 액채정치배양이 각각 우수한 것으로 확인되었다. 본 실험의 결과를 통해 글라디올러스 ‘Topaz' 캘러스로부터의 재분화를 위한 최적 배양 조건을 선발하는 한편, 액체진탕배양법에 의해 기관-캘러스 혼합체를 유도할 수 있었으며, 이를 이용하여 기내 유식물체를 대량생산할 수 있는 가능성을 확인할 수 있었다.

• Biomolecules & Therapeutics
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• 제10권4호
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• pp.281-286
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• 2002

In this study, we intended to get a preliminary data for establishing rat tracheal surface epithelial(RTSE) cell culture system as an experimental model for physiology and pharmacology of tracheal epithelial cells. Primary culture on the membrane support and application of the air-liquid interface system at the level of cell layer were performed. The cell growth rate and mucin production rate were measured according to the days in culture. The results were as follows: this culture system was found to manifest mucocilliary differentiation of rat tracheal epithelial cells, the cells were confluent and the quantity of produced and released mucin was highest on culture day 9, the mucin was mainly released to the apical side and tbe free $^3{H}$-glucosamine which was not incorporated to process of synthesis of mucin was left on the basolateral side. Taken together, we suggest that air-liquid interface culture system can be used as a substitute for immersion culture system and as an experimental model for in vivo mucus-hypersecretory diseases.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.30-41
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• 2011

본 연구에서는 고추의 나출 소포자 배양 시 배양 중 새배지의 첨가와 진탕이 배의 생산에 미치는 영향을 조사하였다. 액체-2층 배양에서 초기 액체배양 시에 새 배지를 첨가하면 배의 발생과 발달 모두 크게 증가하였다. 가장 효과적인 첨가 시기는 초기액체 배양을 시작한 5일 후 이었다. 한편 액체-2층 배양에서 후기 2층배양 시의 새 배지 첨가는 초기 액체배양 시 첨가 때에 비해 그 효과가 적었다. 액체-2층 배양에서 후기 2층배양 시의 1주간 진탕은 정상 자엽배 생산에 효과적이었다. 액체배양시에도 배양 1주 후의 1주간 진탕은 배의 발달에 효과적 이었다. 그러나 액체-2층배양 시와 액체배양 시 모두 진탕기간이 2 ~ 3 주간으로 길어질 때에는 배의 발달이 비정상적이었다. 본 실험 결과 얻어진 정상 자엽배들은 재분화 배지에 이식 시 용이하게 유식물체로 발달하였다. 재분화 식물체들 중에는 반수체와 배가반수체가 혼재하였으며, 이들 간에는 공변세포 내 엽록체 수의 차이가 뚜렷하였다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산할 수 있는 소포자 배양 시스템을 확립하는 데 중요한 기초자료가 될 것이다.

• 한국잠사곤충학회지
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• 제37권2호
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• pp.172-175
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• 1995

흰굳음병균(B. basiana)을 이용하여 백강잠의 대량생산과 효과적인 사상균 살충제 개발을 위해, 사상균의 대량배양 체계에 있어서 접종원인 단균사의 대량 확보에 필수적인 1차 액제배지를 개발하였다. 농가부산물인 저가의 밀기울을 탄소원으로, 질소원으로는 대두박을 선발하고, 이들의 조성비와 농도에 따른 밀기울·대두박 배지에서 단균사의 성장을 조사한 결과, 밀기울과 대두박을 3:2의 비율로 최종배지 농도가 5%되게 조성하였을 때, 배양 4일째 배지 ml당 약 7.6X108개의 단균사를 얻을 수 있어, 단균사의 성장과 경제성면에서 가장 효과적이었다.

• 생약학회지
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• 제27권4호
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• pp.301-308
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• 1996

Hairy roots induced from stems of Rubia cordifolia var. pratensis were cultured in the liquid medium under a variety of auxins to find the optimal condition for the growth and production of pigments. Culture of the hairy roots on NN liquid medium containing NAA 0.5 mg/l was best for growth of hairy roots. Production of yellow anthraquinone derivatives and purpurin in hairy roots was enhanced by the culture on NN liquid medium without auxins. Effects of L-phenylalanine, L-tyrosine and juglone, synthesized via the shikimic acid pathway, on growth and production of pigments in hairy roots were studied in the present study. Concentration of exogeneous L-phenylalanine. L-tyrosine and juglone in liquid culture system of hairy root containing NAA 0.1 mg/l was decreased quickly in its early stages of the culture period. Addition of juglone to NN liquid medium containing NAA 0.1 mg/l enhanced the productivity of pigments in hairy roots.

• 한국환경보건학회지
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• 제40권2호
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• pp.127-136
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• 2014

Objectives: This study was conducted to investigate the removal of high odor concentration from swine wastewater treatment facility by full scale biofilter using liquid with deodorant mixed with earthworm cast and distillery sludge. Methods: The supply of the culture liquid to the microorganism on the media in the biofilter increases the activity and growth of biomass. The experimental equipment was biofilter tower with treatment capacity of 90 m 3/min. The experimental conditions included gas flow of $60m^3/min$, retention time of 20 sec, and gas/liquid ratio of 67. Results: With changing season from winter to summer, the inlet odor concentration of ammonia increased from 2.5 ppm to 29 ppm, and of hydrogen sulfide from 21 ppm to 91 ppm, respectively. The odor treatment system with biofilter using the culture liquid was stable when the high loading rate increased and showed excellent removal grade with an average of 96.7% for ammonia, and an average of 93.7% for hydrogen sulfide. The pH and SCOD in the recirculating culture liquid near the bottom of the biofilter tower decreased with operation time, but its influence on the odor removal rate was negligible, because the organic matter (SCOD) was replaced by some culture liquid supplied 2-4 times per day. Conclusions: The biofilter using culture liquid could successfully remove high odor concentration which was generated from swine wastewater treatment facility.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.212-212
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• 2017

This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.85-94
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• 2010

In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.