• Title/Summary/Keyword: Live NDV vaccine

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Studies on the immunization against field strain after live Newcastle disease virus vaccination (뉴캣슬병 생독백신 접종 후 야외 분리 바이러스에 대한 면역성 조사)

  • 김순태;박인화;김성국;김영환;조광현;손재권
    • Korean Journal of Veterinary Service
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    • v.24 no.2
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    • pp.147-159
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    • 2001
  • This Study was conducted to determine vaccination programs for the control of Newcastle Disease(ND) in chickens and investigate protective effect against Newcastle disease virus (NDV) after live ND vaccination. Maternal HI antibody titer level of chickens according to day(age) 1, 7, 14, 21, 28 and 35 were decreased gradually as 7.10$\pm$0.74, 6.57$\pm$0.74, 3.71$\pm$1.25, 2.20$\pm$1.03, 1.20$\pm$1.23 and 0.50$\pm$0.71. As a result of HI test and ELISA, both chickens vaccinated with VG/GA strain live vaccine at 1-day-old and chickens not vaccinated do not have antibody titer for protection against NDV at 14-day-old. Except for LaSota strain vaccine, in case of vaccination with VG/GA spray and VG/GA, B1 and LaSota strain drinking water at 14-day-old, the protective effect was 100% in chickens inoculated NDV($10^{7.2}$ $EID_{50}$/50${\mu}\ell$, eye drop) at 21-day-old, but not 10~50% at 28-day-old. These data suggest that live NDV vaccination should be given at 10-day-old 20-25day-old for protect against NDV at periodic outbreaks of ND caused by velogenic viscerotropic NDV in the environment of a farm.

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Production of Newcastle vaccine using continuous mammalian cells

  • Gwak, Il-Yeong;Choe, Yeon-Suk;Jeong, Yeon-Ho;Jeon, Gye-Taek;Kim, Ik-Hwan
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.281-284
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    • 2002
  • Specific pathogen free (SPF) eggs have been used to produce live vaccines. however, their application causes many problems such as cost, space and waste disposal. The substitution of mammalian cells for SPF eggs offers a desirable system of vaccine production. In this study, mammalian cells were tested for the infection of Newcastle disease virus (NDV). As a result, DF-I and MDBK cells showed high virus productivity compared to the other mammalian cells. For the highest productivity of NDV, the optimal multiplicity of infection (M.O.I.) in DF-I or MDBK cells was determined to be 0.2 or 0.5 M.O.I., respectively.

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Evaluation of the recent live vaccination effects against Newcastle disease under field conditions (최근 야외농장에서 실시하고 있는 뉴캣슬병 생독백신 접종효능에 대한 평가)

  • Song, Chang-seon;Lee, Youn-jeong;Han, Myung-guk;Seong, Hwan-woo;Kang, Kyung-soo;Lee, Joong-bok;Kim, Jae-hak
    • Korean Journal of Veterinary Research
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    • v.40 no.3
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    • pp.563-573
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    • 2000
  • Periodic outbreaks of Newcastle disease (ND) caused by velogenic viscerotropic ND virus (vvNDV) has become a major concern in Korea nowadays. Throughout last epidemic, the winter season in 2000, most chicken flocks infected early, under 2-4 weeks of age, showed high mortality up to 50-100%. Serum samples collected from 201 breeder, 284 layer and 112 broiler chicken flocks were examined to evaluate the efficacy of various vaccination methods and programs routinely used for mass vaccination in the field poultry farms. Despite repeated live vaccination, most poultry flocks vaccinated by drinking water route using nipple water supply system failed to produce solid active immune response to NDV during the growing time. In the present study, we applied the spray vaccination technique using Ulvavac or Desvac sprayer to the experimental poultry flocks and examined the efficacy of live vaccination effects induced by it under field condition. Measurable antibody to NDV as well as early protection against vvNDV challenge were found in poultry flocks vaccinated by spray route. Further, we did not found significant post vaccination reactions caused by spray vaccination if properly administered. These data indicate that the spray vaccination will be safe and reliable mass vaccination method for the prevention of ND.

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Efficacy of Newcastle Disease Virus $B_1$Vaccine in Quails (메추리에서의 Newcastle균 바이러스 $B_1$백신의 효능시험)

  • 박형철;최정옥
    • Korean Journal of Poultry Science
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    • v.19 no.1
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    • pp.17-25
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    • 1992
  • The efficacy of B$_1$l live vaccine which is used successfully in chicken was examined against Newcastle disease in quails. A total of 480 male quails were divided into 4 groups, of which 3 groups were vaccinated via drinking water, eye instillation and spraying method and the remaining was employed as a nonvaccinated control group. At 3 weeks after the first vaccination a part of quails in each group was revaccinated. Efficacy of the vaccine was evaluated by the antibody responses and the protection rates after challenge with a virulent NDV. Vaccination of quails with $B_1$ NDV at 10 days of age resulted in beneficial effect compared to nonvaccinated group regardness of vaccination methods adopted although general protection rates were considerably low. Twice vaccination gave higher protection than once vaccination. Hemagglutination inhibition antibody responeses were significantly higher in groups of quails vaccinated by spray and eye instillation method than by drinking water administration. Antibody responses were marked at 2 weeks onward and until 5 to 7 weeks after vaccination. Antibody responses were rapid and marked after second vaccination. However, antibody level did not last longer than 5 to 7 weeks postvaccination. Vaccine caused no adverse effect on quails in terms of growth'rate, body temperature or clinical signs.

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Efficacy of Concurrent Administration of Live and Killed Newcastle Disease Vaccines in Day - Old Broiler Chicks (육용계 초생추에 대한 뉴켓슬병 생독 및 사독 백신의 동시접종 효과)

  • 최정옥;박승주;위성하
    • Korean Journal of Poultry Science
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    • v.15 no.3
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    • pp.193-198
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    • 1988
  • In order to examine the efficacy of concurrent vaccination with live and killed Newcastle disease(ND) vaccines two types of each live($B_1$ and LaSota) and killed(gel and oil) vaccines of all commercial origin were administered either alone or simultaneously to day-old broiler chicks having maternal antibody. Live vaccines were given by conjuntival instillation in volumes of 25${\mu}\ell$ containing $10^{6.0}$ to $10^{6.3}$ median embroy infective dose(EH)) while killed vaccines were given in 0.3$m\ell$ volumes subcutaneously at the back of the neck Hemagglutination inhibition(HI) antibodies were determined at weekly intervals until 8 weeks of age and protection rate was determined at 4 and 8 week of age by challenge inoculation with virulent ND virus(NDV). During the 8 weeks experimental period concurrent administration of live and oil vaccine produced the highest level of HI antibody and the most satisfactory protection, whereas concurrent rent vaccination with live and gel vaccine induced poor immune responses. There was no noticeable difference in the efficacy between the live vaccines, Bl and LaSota when simultaneously administered with oil vaccine. Except for oil vaccine, single administration of either live or killed vaccine at day-old produced less than 50% protection at 4 and 8 weeks postvaccination(PV). Oil vaucine alone induced 80% and 70% protection at 4 and 8 week PV, respectively. Concurrent vaccination caused on visible side reaction like respiratory symptoms and did not negatively influence the growth rate of birds until the end of experiment.

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