• Plant Biotechnology Reports
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• v.5 no.1
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• pp.61-69
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• 2011

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.89-90
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• 1999

페레니얼 라이그라스(Lolium perenne L.)의 질소공급형태에 따른 저장질소의 함량과 재생기간 중 저장질소의 이용 및 재생활력을 구명하고자 수경재배 하였다. (NH$_4$)$_2$SO$_4$, KNO$_3$, Urea, Organic-N 및 NH$_4$NO$_3$의 서로 다른 질소형태로 각각 1mM/week을 4주간 공급하여 건물생산량을 보았고, 예취 후 각각의 질소 형태로 4mM/40days동안 공급하여 흡수된 질소의 함량 및 잔여기관내 축적된 질소의 함량을 관찰하였으며, 예취 후 저장된 질소에 의한 재생활력을 보기 위해 0.2mM NH$_4$NO$_3$의 형태로 동일하게 공급하였다.(중략)

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.19-24
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• 2000

This study was camed out in order to establish plant regeneration via seed-derived callus of perennialryegrass.Varietal difference in callus growth and plant regeneration was obvious between two cultivars of perennialryegrass. "Reveille" showed a relatively high capacity for plant regeneration. The MS medium was superiorto SH or Bj in callus formation and plant regeneration. The highest regeneration frequency (60%) from calluswas obtained in presence of 5 mg/ l 2,4-D and 1 mg/ t kinetin. Regeneration response varied among calluscultures initiated from the same cultivar. Regeneration frequency was the most effective in 6 weeks-old calliafter initiation and lost their regeneration capacity gradually over a period of 12 weeks.(Key words : Perennial ryegrass (Lolium perenne L.), Plant regeneration)e L.), Plant regeneration)

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.83.1-83
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• 2001

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.97-97
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• 2001

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.235-240
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• 2007

Optimum tissue culture conditions for an efficient induction of embryogenic callus from mature seeds of perennial ryegrass (Lolium perenne L.) and regeneration of plants from callus tissues were investigated. MS medium containing 3 mg/L 2,4-D and 0.1 mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (58.3%) was observed when the embryogenic callus tissues were cultured on N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be helpful for molecular breeding of perennial ryegrass through Agrobacterium-mediated genetic transformation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.89-96
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• 2001

Carbohydrate and soluble protein reserves and regrowth characteristics in response to cutting height were investigated over four regrowth cycles of turf-type perennial ryegrass(Lolium perenne L. cv. preludeII). When the plants were at the full-vegetative stage (twelve weeks-old), three sequential defoliations at 3, 6 and 9 cm above the root base were imposed at 2-week intervals. Shoot dry weight in all three treatments continuously decreased with progressing regrowth cycle and the decreasing rate was higher as cutting height was lowered. TNC (total non-structural carbohydrate) in stubble at the end of the fourth regrowth cycle in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 27%, respectively, comparing the initial content. TNC in roots also largely decreased with similar pattern in response to cutting height, whereas the absolute amount was much less compared to stubble. Soluble protein in stubble in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 57%, respectively, at the end of fourth regrowth. A significant correlations between TNC (r=0.906) or protein (r=0.879) at the fourth defoliation and dry weight of regrowing shoots at the end of fourth regrowth were observed. these results indicated that cutting height closely influences the levels of organic reserves available for new growth, and that the levels of reserves might provide a useful tool as a determinant for regrowth dynamics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.15-20
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• 2001

Plant growth regulators were treated on the cut perennial ryegrass (Lolium perenne L. cv. Reveille) to investigate the effect on the regrowth after cutting. The growth showed better result when 0.1 or 0.5 mg/L cytokinin were treated. Among cytokinins, kinetin or 2iP gave the better effect on the growth than BAP. In 2.4-D as an auxin. cut plants grew best at the concentration of 0.1 mg/L. The initial regrowth was very vigorous when GA$_3$ was treated as a growth regulator, but the growth was retarded after 2 weeks later of cutting. Co-treatment of kinetin as a cytokinin and 2,4-D as an auxin showed synergistic effect on the regrowth of cut perennial ryegrass. Both plant growth regulators gave the same result at the same concentrations in the suspension culture of perennial ryegrass cells.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.88-88
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• 2001

• Plant Resources
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• v.6 no.2
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• pp.114-119
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• 2003

In this study, an on-site survey was conducted around Gasado island at Jodo-myeon, Jindo-gun, Jeonnam during July 2 to 25, 2002. The naturalized plants investigated in the surveyed sites were revealed 15 families and 39 species, including Althaea rosea and Oxalis articulata, which are ornamental plants. Only 10 species appeared in Imjado island among the surveyed sites the surveyed sites and Imjado Island, including Elymus sibiricus, Lolium perenne, Chenopodium album, Oxalis articulata, Oxalis corymbosa, Oenothera odorata, Xanthium strumarium, Bidens frondosa, Ambrosia artemisiaefolia var. elatior, and Taraxacum officinale. 6 species were distributed only in Gasado island, Jindo-gun, which included include Festuca myuros, Chenopodium ambrosioides, Amorpha fruiticosa, Malva neglecta, Calendula arvensis, and Carthamus tinctorius. 18 species were showed in Soheuksan island.; Yeosu area, 29 species; Imja Island in Sinan-gun, 34 species, and Gasado Island in Jindo-gun revealed 39 species. It is believed that the species of naturalized plants have been expanded compared to the other areas because of close location to the inland and large population fastiy. The Urbanization Index(UI) of this area was found to be 19.5. The naturalized plant communites in these surveyed sites were classified into Rumex crispus, Elymus sibiricus, and Lolium perenne community according to the vegetation table. Furthermore, they formed a community around areas that received great artificial influence from human dwellings or roads due to the characteristics of species distribution