• Journal of Life Science
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• v.6 no.4
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• pp.293-298
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• 1996

Thirteen subspecies of Bacillus thuringiensis including B. t. israelensis, B. t. morrisoni PG-14 and B.t. darmstadoemsos known to be toxic to dipteran insects were treated on the mushroom (Flammulina velutipes) compost to estimate the biological control effect of a sciarid fly, Lycoriella sp. According to the results, it was found that there were no significant effects of the tested strains of B, thuringiensis on the control of Lycoriella sp. For further confirmation, larval gut juice of Lycoriella sp. and trypsin were respectively treated into the parasporal crystal proteins of three subspecies of B. t. israelensis, B. t. morrisoni PG-14, and B. t. darmstadiensis. The proteins were separated by SDS-PAGE. According to the results, the major parasporal crystal proteins were respectively produced by B. t. morrisoni as the amount of 52 kd, B. y. israelensis as 37kd and B. t. darmstadiensis as 39kd, but the activity of these proteins could not be unfortunately confirmed in this study.

• Korean journal of applied entomology
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• v.35 no.1
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• pp.45-51
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• 1996

As the pest of the Letinus edoeds, five insect species and six animal species are investigated injuring the fresh mushroom. Of these, insect and animals, Achorutes armatus, Scaphidium amurense and Philomycus confusa caused severe damage to mushroom fruitbody, and a greate deal of damage was done to mushroom lamella by mites (Rhizoglyphis sp., and Histiostoma sp.). As the pest of Lentinus edodes bed logs, two insect species from immature bed logs, eight insects and one isopod species from matured bed logs were investigated. Of those, Moechotypa diphysis was found to be the most injurious species from both immature and matured bed logs. As the pest of Pleurotus ostreatus, six insects and the two mite species were investigated from sporophore. Of those, Lycoriella sp. and Mycophilla sp. are considered to be very serious. while two insects and five mite species were investigated from the mushroom bed. Of those, one insect (Lycoriella sp.) and two mites (Histiostoma feroniarum and Throglyphus bankslogior) found to be abundant and caused serious damage.

• Korean journal of applied entomology
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• v.38 no.1
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• pp.41-46
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• 1999

This study was carried out to investigate the seasonal and regional occurrence and development of major pest flies on the oyster mushroom. The survey was performed in the oyster mushroom houses located at 16 places, selected randomly throughout South Korea from July to August and from October to November in 1997. The six species of flies were found including three pest species from Sciaridae, Cecidomyiidae and Scatopsidae flies. The pattern of occurrence was somewhat dissimilar depending on the survey periods and regions. Among these, the most serious and abundant pest, Lycoriella mali (Diptera: Sciaridae), occurred year around at the regions surveyed. In this study, Coboldia fuscipes (Diptera: Scatopsidae) was confinned as a mushroom pest. Its larvae damaged the mycelium, and occurred usually in summer from July to August. The paedogenetic Mycophila sp. (Diptera: Cecidomyiidae) was found abundantly on the oyster mushroom bed, and caused a severe damage in fall from October to November.

• The Korean Journal of Mycology
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• v.27 no.4 s.91
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• pp.289-292
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• 1999

We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipes, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of seperating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was $30{\sim}140$ mesh; Lycoriella mali was trappped mainly $30{\sim}65$ mesh sieve, Coboldia fuscipes was caught mainly $30{\sim}100$ mesh sieve, Histiostoma sp. was trapped mainly $65{\sim}140$ mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.77-82
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• 1997

This study was carried out to investigate the developmental characteristics and life cycle of sciarid fly (Lycoriella sp.), an important mushroom pest. The sciarid fly was reared ina growth chamber at 21$\pm$$1^{\circ}C$ and 55$\pm$5% of relative humidity with a 16h high, 8h dark cycle and Flammulina velutipes was used at diet. The egg was oval with 0.17 mm in diameter and 0.27 mm in length. Average egg period was 4 days. Body length of each instar from the 1st to 3rd was 0.7, 1.5, and 4.5, mm. respectively. Average weight of each instar was 1.9, 15.4 and 93.6 $\mu\textrm{g}$, respectively. In the 4th instar larvae, however, developmental characteristics of female and male became different by body length, weight and segment size. Body length and weight of the 4th instar female larvae were 5 mm and 162$\mu\textrm{g}$, and those of the male larvae were 3.5 mm and 90 $\mu\textrm{g}$, respectively. The larval periods of female and male were respectively 13.5 and13 days. Pupal length of female and male was 3.5 and 2.7 mm, and average weight was 136 $\mu\textrm{g}$ and 6.5$\mu\textrm{g}$, respectively. Adults were emerged from female and male pupae on 5.5 and on 5 days after pupation, respectively. Average longevity of male and female were 7 and 5 days, respectively. The weight of female was two-fold heavier than the male. After copulation, female laid approximately 130 to 150 eggs on/in the sporophore of mushroom. In conclusion, total lifespan of male and female were 29 and 28 days, respectively.

• Journal of Mushroom
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• v.11 no.4
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• pp.201-207
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• 2013

Fungus gnats are usually found in mushroom farm and have recently become important pest because they can cause severe damage and reduce the production on shiitake mushroom. Usually shiitake mushrooms are cultivated on both oak bed logs and in the artificial sawdust beds in greenhouses. Using yellow sticky trap, the dipteran species in shiitake mushroom farm were collected from May to September in Kyonggi-do and Chungcheong-do in 2013. To identify the main species of fungus gnat on the shiitake farm in Korea, the collected samples were determined the sequence of cytochrome c oxidase subunit I (COI) by DNA barcoding. The phylogeny based on maximum likelihood analyses from COI sequence showed that Bradysia difformis and B. alpicola were main species of shiitake bed log and Scatopsidae sp. and B. difformis were dominant species of sawdust beds.