• Journal of Science Education
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• v.36 no.2
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• pp.313-328
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• 2012

This study was conducted for the purpose of making the better utilization of MBL in class, based on 2009 curriculum which emphasizes research activities and recommends the direct use of the MBL. We investigated primary, secondary and pre-service teachers' satisfaction and perception level after conducting training about making good use of MBL. The satisfaction level of the training turned out to be high, level of applicability of MBL, expected improvement in learning skills of students and the will to apply it in class was high. The answer that they expect MBL to increase students' curiosity and interest in science was the highest among the survey results, which means that MBL could be used as a solution to lack of students' interest in science. Besides, primary teachers than secondary and pre-teachers, long careered teachers than short careered teachers and MBL-experienced teachers than inexperienced teachers showed more satisfaction and the will to adapt MBL overall. Primary and pre-teachers hoped MBL training to be more related to STEAM education, whereas secondary teachers wanted the training to have more to do with increasing creativity If advanced MBL training program is opened. The price was chosen as the best obstacle to MBL class' application, and the lack of manual for experiment and education to teacher was also pointed out secondly. In conclusion, if MBL is fully equipped in school and training on how to take advantage of it is provided continually, It is expected that MBL could increase the utilization in the field of science education. The results of this paper can be used when you configure the MBL utilization training program.

• Journal of The Korean Association For Science Education
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• v.27 no.1
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• pp.59-69
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• 2007

The aim of this study was to investigate teachers' perceptions on MBL (microcomputer-based laboratory) experiment training program for teachers, the expecting effects of MBL experiment and application of MBL experiment after conducting MBL experiment training for science classes in schools. This study showed that most of the teachers who participated in the training program thought that the MBL experiment training program was very useful and instructive. Many teachers considered that MBL experiments using a computer could decrease time spent in the experiment by accurate and fast data collection and analysis. They also thought that the reduced time could be used more effectively in the analysis of experimental data and discussion activities leading to correct concept formation as well as in the development of graphical analysis and science process skills. However, they thought that MBL experiments were ineffective in learning how to operate experiment apparatus. This study also revealed that most teachers intended to apply MBL experiments in real classrooms context right after the training course and they pointed out many obstacles in introducing MBL experiments into their classrooms such as a budget to purchase equipment, poor laboratory conditions, and few MBL experiment training opportunities. In order to apply MBL experiment into the real classrooms, further changes were suggested as follows; development of technologies to reduce unit cost of equipment for MBL experiments, production and supply of many kinds of sensors, development of MBL experiment materials, and expansion of the training program for teachers.

• Journal of Life Science
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• v.23 no.3
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• pp.347-354
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• 2013

Innate immunity is the ability to differentiate infectious agents from self. The innate immune system is comprised of a complicated network of recognition and effector molecules that act together to protect the host in the early stage of an infectious challenge. Mannose-binding lectin (MBL or mannose-binding protein, MBP) belongs to the family of $Ca^{2+}$-dependent lectins (C-type lectin with a collagen-like domain), which are considered an important component of innate immunity. While it is associated with increased risk and severity of infections and autoimmunity, the most frequent immuno-deficiency syndrome was reported to be low MBL level in blood. Deficiency of human MBL is caused by mutations in the coding region of the MBL gene. Rat homologue gene of human MBL gene was used to study functions of wild type and mutant MBL proteins. Although extensive studies have yielded the structural information of MBL, the functions of MBL, especially mutant MBL, still require investigation. We previously reported the cloning of rat wild-type MBL gene and the production of a truncated form of MBL protein and its antibody. Here, we present the cloning of mutant MBL cDNA in collagen-like domain (R40C, G42D, and G45E) using site-directed mutagenesis and differential behaviors of wild type and mutant MBL in cells. The major difference between wild type and mutant MBL was that while wild type MBL was secreted, mutant MBL was inhibited for secretion, retained in endoplasmic reticulum, and still functioned as a lectin.

• Journal of Life Science
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• v.19 no.2
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• pp.284-288
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• 2009

The innate immune system is important for the first line of host defence against infectious agents, which have penetrated the mechanical barriers. Mannose-binding lectin (MBL or mannan-binding protein, MBP) is a serum protein that is synthesized in the liver as a part of the acute phase response. MBL binds to carbohydrate structures presented by a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL is synthesized as a monomer that has a carboxy-terminal carbohydrate recognition domain, a neck region and a collagen region. Low MBL level was reported to be the most frequent immuno-deficiency syndrome. Although extensive studies have yielded detailed information on the structure of MBL, functions of the MBL complex are not fully understood yet. We, here, present cloning process of MBL cDNA from the rat liver and production of truncated recombinant MBL protein using a bacterial expression system in order to produce anti-MBL polyclonal antibody. Anti-MBL polyclonal antibody was raised in a New Zealand rabbit and its affinity was tested against recombinant protein using western blot technique. MBL cDNA, recombinant protein and anti-MBL antibody could be used as great arsenals to dissect cellular biochemistry of MBL.

• Journal of Life Science
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• v.20 no.9
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• pp.1420-1425
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• 2010

Innate immunity is the first line of host defense consisting of various molecules against infectious challenges. Mannose-binding lectin (MBL) belongs to the collectin protein family which takes part of innate immunity and is able to recognize specific carbohydrates on the surface of a variety of infectious agents acting as a pattern recognition molecule. In this way, MBL differentiates self from non-self and interacts with other molecules of the immune system. MBL genotype shows various MBL2 polymorphisms which are responsible for MBL deficiency in a substantial portion of the entire human population and for susceptibility to infectious disease. Therefore, it has been highlighted in the relationship between genetic variants and clinical significance. Here we focus on presenting anoverview of our understanding of MBL structure and functions.

• Journal of the Korean earth science society
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• v.31 no.6
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• pp.637-646
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• 2010

The purpose of this study was to investigate the effects of Microcomputer Based Laboratory (MBL) on the middle school students' graphing skill and science process skill in Earth Science classes. MBL teaching materials for inquiry-based instructions were developed to teach five experimental topics related to the middle school 'Earth Science.' Thirty three middles school students participated in this study, and the pre- and post-tests were conducted. Results indicated as follow: First, MBL classes were effective in developing the graphing skill of middle school students. They were efficient both in graph construction and graph interpretation, especially in data transformation. Second, MBL classes were effective in helping participants to use science process skills, especially, in the subordinate areas including formulating hypotheses, making operational definition, graphing, and interpreting data.

• Journal of Nutrition and Health
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• v.15 no.4
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• pp.249-257
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• 1982

The aim of this study was to observe the relationships between the amount of menstrual blood loss (MBL) and age, parity, number of pregnancy and induced abortion, and the iron nutriture in married Korean women. Fifty-one healthy women aged 26 to 48 years were tested for their MBL, hemoglobin(Hb) concentration, hematocrit (Hct) value, mean corpuscular hemoglobin concentration (MCHC), serum iron and ferritin concentrations. The results of this study are summarized as follows : 1) The mean value and standard error of mean of the MBL was $32.0{\pm}3.94ml$, and the range of MBL was 3.8-127.2 ml for total subjects. Maximum number (23.5%) of the subjects fell in the group with MBL of 10-l9ml, while 17.7% showed MBL above 50m1. 2) There were no statistically significant differences on the mean amount of MBL between the age groups 25-48 years. 3) The mean amount of MBL in 1-2 and 3-4 para groups were 28.5 and 36.1 ml, respectively, but the difference between two groups was not significant 4) It was observed that the mean amount of MBL was gradually decreased as the number of pregnancy and induced abortion were increased, but the significance was not observed. 5) The mean values of Hb concentration, MCHC and serum iron concentration were slightly decreased in subjects with MBL of more than 50ml but the statistical significance was not observed. The mean of Hct value was not influenced by the amount of MBL. While serum ferritin concentrations were markedly decreased and the prevalence rate of anemia was markedly increased as the amount of MBL was increased. There was significant difference (P<0.05) between the mean amount of MBL with respect to the serum ferritin concentration.

• Journal of The Korean Association For Science Education
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• v.28 no.1
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• pp.67-74
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• 2008

The purpose of this study was to investigate the effects of an MBL instrument in laboratory execution by analyzing an experimental time and student to student interactions in the MBL and traditional method of the acid-base titration experiment. The MBL method used a conductivity sensor and the traditional method used a current meter on the HCl/NaOH titrations. In comparison of the two methods, the traditional method required more time than the MBL and most of the extra time were used in the execution. In the execution, the MBL method showed more numbers of student to student interaction and higher level of verbal interaction than the traditional method.

• Journal of The Korean Association For Science Education
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• v.22 no.5
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• pp.957-969
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• 2002

Theoretical benefits and research findings on the use of Microcomputer-based Laboratory (MBL) are considered for using MBL in a way that will be of benefit to students and teachers, and discussed as a whole for further synthesis, including the formulation of a research agenda for future consensus-based action. Based on the findings obtained from a comprehensive review of the literature, using a systematic approach, the uses of MBL were compared and contrasted for advancing understanding of the teaching and learning processes in science and mathematics. A number of benefits were proposed by MBL developers but not investigated by educational researchers. A few research studies considered the following practical aspects raised by classroom science teachers: technical problems of MBL equipment; inaccuracy or incompleteness of presentation; efficient ways for handling class time with MBL instruction; and development of MBL curriculum materials for their own instruction. This lack of research related to the use of MBL in science classrooms resulted in educational research that was neither respected nor utilized by science teachers. Setting a research agenda based on the theoretical benefits and research findings is necessary for the effective use of MBL in science classrooms can help to maximize the prospects for successful school improvement projects while minimizing the innovation-related frustrations of individuals.

• Journal of The Korean Association For Science Education
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• v.28 no.4
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• pp.331-339
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• 2008

The aim of this study was to compare the effectiveness of microcomputer-based laboratory (MBL) with the traditional laboratory experiment on science classes in middle school. MBL tools were developed and applied for five experiment subjects chosen from middle school science textbook for MBL experiment classes, while usual experiment methods suggested in the textbook were used in traditional experiment classes. In order to evaluate the effects of MBL experiment class, achievement, graphic ability, science process skills, affective aspect related to science were tested before and after applying the MBL experiment. The result revealed that MBL experiment class was more effective than traditional experiment class in improving student's science achievement, science process skills, and graphic ability. The result also indicated that there was a significant difference between experiment and control group with regards to the effect on affective aspect related to science. From the above result, we can find the positive implication of applicability of MBL experiment as a new experiment tool at the early stage of introduction in a real context.