• Environmental Engineering Research
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• 제24권2호
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• pp.271-278
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• 2019

Mass cultivation of microalgae is necessary to achieve economically feasible production of microalgal biodiesel. However, the high cost of nutrients is a major limitation. In this study, corncob extract (CCE) was used as an inorganic and organic nutrient source for the mass cultivation of Chlorella vulgaris (C. vulgaris). Chemical composition analysis of CCE revealed that it contained sufficient nutrients for mixotrophic cultivation of C. vulgaris. The highest specific grow rate of C. vulgaris was obtained at pH of 7-8, temperature of $25-30^{\circ}C$, and CCE amount of 5 g/L. In the analysis using various growth models, Luong model was found to be the most suitable empirical formula for mass cultivation of C. vulgaris using CCE. Analysis of biomass and production of triacyglycerol showed that microalgae grown in CCE medium produced more than 17.23% and 3% more unsaturated fatty acids than cells cultured in Jaworski's Medium. These results suggest that growing microalgae in CCE-supplemented medium can increase lipid production. Therefore, CCE, agricultural byproduct, has potential use for mass cultivation of microalgae.

• Environmental Engineering Research
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• 제19권3호
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• pp.283-287
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• 2014

Cultivation of microalgae using wastewater exhibits several advantages such as nutrient removal and the production of high valuable products such as lipid and pigments. With this study, two types of wastewater from instant noodle factory; mixed liquor suspended solids (MLSS) and effluents after sedimentation tank were investigated for green microalga Scenedesmus sp. cultivation under laboratory condition. Optimal wastewater dilution percentage was evaluated in 24 wells microplate. MLSS and effluent without dilution showed the highest specific growth rate (${\mu}$) of $1.63{\pm}0.11day^{-1}$ and $1.57{\pm}0.16day^{-1}$, respectively, in which they were significantly (p < 0.05) higher than Scenedesmus sp. grown in BG11 medium ($1.08{\pm}0.14day^{-1}$). Ten days experiment was also conducted using 2000 ml Duran bottle as culture vessel under continuous light at approximately 5000 lux intensity and continuous aeration. It was found that maximum biomass density of microalgae cultivated in MLSS and effluent were $344.16{\pm}105.60mg/L$ and $512.89{\pm}86.93mg/L$ respectively and there was no significant (p < 0.05) difference on growth to control (BG11 medium). Moreover, cultivation microalgae in wastewater could reduce COD in wastewater by 39.89%-73.37%. Therefore, cultivation of Scenedesmus sp. in wastewater from instant noodle factory can yield microalgae biomass production and wastewater reclamation using photobioreactor simultaneously.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2012년도 추계학술대회 논문집
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• pp.945-946
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• 2012

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.1501-1502
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• 2011

• 공업화학
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• 제29권1호
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• pp.1-9
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• 2018

미세조류는 바이오연료 생산을 위한 가장 지속가능하고 장래성이 좋은 생산 원료로 여겨지고 있다. 하지만 최근의 몇몇 전과정평가 연구에 의하면 미세조류 바이오디젤 생산, 특히 배양 단계에 많은 에너지가 소요된다는 단점이 있다. 유기탄소, 질소 및 인과 같은 영양분, 그리고 배양에 필요한 용수 비용이 전체 배양 단계의 80%까지 이를 수 있다. 본 총설에서는 최근 미세조류 배양에 필요한 인공배지의 대체용으로 사용 가능성이 높은 하폐수, 유기비료 연소배가스, 유기성 폐기물 등에 대한 최근의 활용 경향과 사용 전략에 대하여 문한 조사를 통해 요약 및 고찰하였다.

• Korean Chemical Engineering Research
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• 제52권1호
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• pp.8-16
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• 2014

미세조류는 이산화탄소를 제거하고 산업적으로 유용한 다양한 이차 대사물질을 생산해 내는 광합성 미생물로 생물산업분야에서 주목 받고 있는 유용한 자원이다. 이러한 미세조류를 실내에서 효과적으로 배양하기 위해서는 무엇보다도 효율적인 광원이 필수적이다. 최근 발달하는 LED광원은 광원의 크기가 작고, 에너지 효율이 우수하며, 특정 파장만 조사할 수 있다는 점 등의 다른 인공광원과 차별되는 많은 장점을 가지고 있다. LED광원을 미세조류의 배양에 적용하는 연구는 최근에 와서야 점차 시도되고 있는 실정이며, 아직까지 실험실 규모의 실험과 대표적인 특정 종 위주의 결과들만 나오고 있어, LED광원을 미세조류의 산업적인 배양에 적용하기 위해서는 더 많은 세부적인 연구 결과가 요구된다. 하지만 LED 조명을 미세조류 배양 분야에 적용하는 것은 효과적인 접근으로 생각되며, BT(Bio Technology) 산업에 새로운 지평을 열 것으로 생각된다. 따라서 본고에서는 최근 연구되고 있는 LED광원을 이용한 미세조류의 배양 현황 및 그 가능성에 대해서 조사하고, 향후 나아갈 방향에 대해서 기술해 보았다.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.630-637
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• 2018

The high rate algal ponds (HRAP) powered and mixed by a paddlewheel have been widely used for over 50 years to culture microalgae for the production of various products. Since light incidence is limited to the surface, water depth can affect microalgal growth in HRAP. To investigate the effect of water depth on microalgal growth, a mixed microalgal culture constituting three major strains of microalgae including Chlorella sp., Scenedesmus sp., and Stigeoclonium sp. (CSS), was grown at different water depths (20, 30, and 40 cm) in the HRAP, respectively. The HRAP with 20cm of water depth had about 38% higher biomass productivity per unit area ($6.16{\pm}0.33g{\cdot}m^{-2}{\cdot}d^{-1}$) and required lower nutrients and energy consumption than the other water depths. Specifically, the algal biomass of HRAP under 20cm of water depth had higher settleability through larger floc size (83.6% settleability within 5 min). These results indicate that water depth can affect the harvesting process as well as cultivation of microalgae. Therefore, we conclude that water depth is an important parameter in HRAP design for mass cultivation of microalgae.

• 한국수산과학회지
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• 제32권2호
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• pp.186-191
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• 1999

본 연구에서는 미세조류의 고밀도 배양을 위한 경제적인 공정을 개발하는 차원에서 3종의 미세조류 즉, Chlorella vulgaris, Dunaliella salina 및 Porphyridium purpureum를 Ca-alginate 로 microencapsulation 한 후 air-bubble column형 배양조에서 고밀도 배양을 시도하였다. $2\%\;CaCl_2$ 농도로 capsule을 제조할 때 capsule의 안정성이 가장 좋았으며, capsule 막의 두께 및 $CO_2$ 공급유무에 따른 미세조류 (Chlorella vulgaris)의 생산성은 거의 차이가 없었다. 한편, 3종의 미세조류 모두 capsule로 고정화시킨 것이 bead로 고정화한 미세조류나 free cell 보다 농도가 높았으며, 특히Dunaliella salina가 microencapsulation에 의한 고밀도 배양 효과가 가장 뛰어나 free cell 보다 약 5배 정도의 생산성을 나타내었다.

• 한국해양바이오학회지
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• 제6권2호
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• pp.118-122
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• 2014

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

• Environmental Engineering Research
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• 제21권3호
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• pp.265-275
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• 2016

In this study, the microalga Neochloris oleoabundans was cultivated in a sustainable manner using diluted anaerobic digestate to produce biomass as a potential biofuel feedstock. Prior to microalgae cultivation, the anaerobic digestate was characterized and several pretreatment methods including hydrogen peroxide treatment, filtration, and supernatant extraction were investigated and their impact on the removal of suspended solids as well as other organic and inorganic matter was evaluated. It was found that the supernatant extraction was the most convenient pretreatment method and was used afterwards to prepare the nutrient media for microalgae cultivation. A bench-scale experiment was conducted using multiple dilutions of the supernatant and filtered anaerobic digestate in 16 mm round glass vials. The results indicated that the highest growth of the microalga N. oleoabundans was achieved with a total nitrogen concentration of 100 mg N/L in the 2.29% diluted supernatant in comparison to the filtered digestate and other dilutions.