• 한국동물학회지
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• 제8권1호
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• pp.11-14
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• 1965

The object to this study is to identify the genus and species of the microfilariae which were recently found in the area of Youngju-Gun. THe identification of the microfilariae was made on the morphologicla aspects. 1. Blood samples were collected through vena punction from known microfilariae carriers living in the newly confirmed filaria endemic area of Youngju0Gun in Jyongsang Pukdo Province. Youngju-Gun is located in the mountainous central part of Korean Peninsula. 2. The following fixation and staining techniques were applied. (1) Fixation by drying in the air, followed by staining with Azeo or Giemsa. (2) Knott's fixation method (2% formalin), followed by staining with Azur II. 3. A comparative study of the body length of the microfilariae after different fixation and staining techniques were applied. (1) Knott's fixation method followed by staining with Azur II : average body length found was 28.4$\mu$. (2) Dry fixation followed by staining with Giemsa : average body length found was 209.4$\mu$. (3) Dry fixation followed by staining with Azeo : average body length found was 205.4$\mu$. 4. The locations of the different body cells were measured in 60 individuals of microfilariae in the wet preparation fixed by Knott's method and stained with Azur II. The distance of the different body cells to cephalic apex of microfilariae was measured and calculated as a percentage of the total body length. The average results are as follows : BNC , 3.04% ; N, 22.74%, EP, 31.4% ; EC, 37.77%, G1 cell , 67.94%; G2 cell, 73.54% : G3 cell , 75.55% ; G4 cell, 77.65% ; AP, 82.02%%. 5. As a result of the above findings the microfilariae found in the above mentioned area could be identified as Brugia malai(BRUG, 1927) BUCKLEY, 1960.

• Parasites, Hosts and Diseases
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• 제32권2호
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• pp.101-110
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• 1994

심장사상충 자충이 합성한 당단백의 분지(분지) 역할을 하는 N-linked high mannose 타입 올리고당의 구조에 대한 조사를 수행하였다. 사상충 자충을 방사선 표식 2-[$^3H$] mmannose를 함유한 배지에서 24시간 배양하였다. 자충으로부터 분리 정제한 당단백을 pronate로 소화시킨 다음. concanavalin A-Sepharose 크로마토그라피하여 분획하였다. 37%의 mannose가 자충 대사에 이용되었으며 pectin chromatography를 사용하여 high mannose 타입의 올리고당을 회수하였다. 이 올리고당을 $endo-{\beta}-N-acetylglucosaminidase$ H 효소로 소화시킨 후 HPLC를 사용하여 highmannose 타입 올리고당의 구성을 분석하였다. 심장사상충 자충이 합성한 high mannose 타입 올리고당의 형태는 $Man_5GIcNAc_2,{\;}Man_6GIcNAc_2,{\;}Man_7GIcNAc_2과{\;}Man_8GlcNAc_2$로 확인되었다. 이와 같이 심장사상충 자충이 체내 합성한 high mannose 타입 올리고당이 일반 척추동물이 체내 합성한 high mannose 타입 올리고당과 구조적으로 일치하였다.

• 대한수의학회지
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• 제42권4호
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• pp.545-550
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• 2002

The raccoon dog (Nyctereutes procyonoides) may be infected by Dirofilaria immitis. However, there has been no report on dirofilarial infection in the raccoon dog in Korea. In this study, we report on D. immitis infection in two wild raccoon dogs captured in the Daejeon area. The two raccoon dogs were referred to the Veterinary Teaching Hospital, Chungnam National University for diagnosis of D. immitis infection. The modified Knott's test for the detection of blood D. immitis microfilariae was positive, and serological test (FASTest$^{(R)}$ HW Antigen ELISA kit, Diagnostik Mega Cor, Austria) for D. immitis was positive as well. Additionally, D. immitis microfilariae were differentiated from other microfilariae by using acid phrnphatase histochemical staining (Leucognost-SP$^{(R)}$kit, Diagncstica MERCK, Germany). The two raccoon dogs were necropsed and D. immitis infection was confirmed.

• 대한수의학회지
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• 제30권4호
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• pp.473-478
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• 1990

This survey was conducted to observe the prevalence of blood parasites in slaughtered cattle included Korean native cattle, Charolias, Hereford, Aberdeenangus and Holstein breeds in the Western area of Kyeongnam. The results obtained are summarized as follows: 1. The prevalence of T sergenti was shown 71.8% as 395 heads of a total of 550 heads examined and from Jaunary to November the monthly prevalence of T sergenti was shown the range of 61.1% to 84% except 38.5% in December. The other blood parasites included Babesia and Anaplasma were not detected from the blood samples except Setaria spp microfilariae. 2. The distribution of parasitaemia levels of T sergenti in positive cattle was shown 93.9% in the range of 1~10/1000 rbc, 4.1% in 11~20, 1.3% in 21~30 and 0.8% above the range of 31. 3. The pervalence of T sergenti by breeds of slaughtered cattle was shown 71.2% in Korean native cattle, 72.7% in Charolias, 78.3% in Hereford and 81. 8% in others (Aberdeen-angus and Holstein) respectively. Also the parasitaemia levels in these cattle were shown higher levels in imported cattle included Charolias, Hereford, Aberdeen-angus and Holstein breeds comparing with Korean native cattle. 4. The prevalence of Setaria spp microfilariae in slaughtered cattle was shown 6.9% and by monthly prevalence of the parasite was shown higher in March, April and May compared with June, July, August and October. But in the winter season included January, February, November and December the parasite was not detected from the blood samples. 5. The distribution of parasitaemia levels of Setaria spp microfilariae per ml of blood was shown 65.8% in the range of 1~50, 13.2% in 51~100 and 10.5% in 101~200 and above the range of 201, respectively.

• Parasites, Hosts and Diseases
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• 제36권4호
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• pp.235-239
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• 1998

1998년 6월과 7월 두 달간에 걸쳐 한국의 한 공군기지의 3-9세령의 군용견 35마리에서 스크리닝한 10마리의 개심장사상충 자연감염 개의 이정맥으로부터 2시간마다 72시간 동안 채혈하여 0.1ml 혈액의 후층도말 brilliant cresyl blue 표본을 만들어 미세사상충을 동정한 다음 그 수를 계산하였다. 이 실험은 일주일 간격으로 두번 실시하였다. 말초혈액에서 검출된 총 미세사상충의 2시간 간격 평균치에 대한 2시간 간격의 자충수의 백분율을 산출한 바 21시에 최고, 11시에 최저이었으며, 비록 최고치는 최저치의 2.17배로서 낮지만 일봉성 야간정기출현성이 인정되었다 (점근성, 95%).

• 한국동물위생학회지
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• 제30권1호
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• pp.155-164
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• 2007

This study was conducted to investigate the prevalence of canine heartworm infections, canine brucellosis and hematologic values from 153 free roaming dogs in the area of Gwangju city from March to November 2006. Nineteen (12.4%) of 153 samples tested with modified Knott's technique showed positive reaction for microfilariae. Polymerase chain reaction using specific primers for D immitis amplified the expected product from all samples of 19 microfilaremic canine blood samples as determined by the modified Knott's test for microfilariae. The seasonal infection rates of microfilariae were higher in the spring season (10/19, 52.6%) than in the other seasons. The major hematological findings in microfilaremic dogs were mild leukocytosis and mild monocytosis. A total of 100 dogs randomly selected from 153 free roaming dogs were negative for canine brucellosis by serological test using immunochromatographic antibody test kit.

• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.759-761
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• 2013

Lymphatic filariasis is a common parasitic disease of cats in tropical regions including Thailand. The objective of this study was to determine the efficacy of ivermectin against microfilariae of Brugia pahangi in naturally infected cats. Eight cats naturally infected with B. pahangi were divided into control (untreated) and treated groups. Cats in the latter group were given ivermectin injection at 400 ${\mu}g/kg$ weekly for 2 months. Microfilariae were counted every week until 48 weeks. Microfilaremia was significantly decreased in the treated group 4 weeks after starting the treatment and become zero at week 9 and afterwards. On the other hand, cats in the control group had high microfilaremia throughout the study. It was successful to treat and control B. pahangi infection in naturally infected cats using ivermectin.

• 대한수의학회지
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• 제43권3호
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• pp.517-520
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• 2003

Prevalence of microfilariae, Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema dracunculoides from 506 German Shepherd dogs reared in Korea was investigated by modified Knott's test to detect circulating microfilaria and by acid-phosphatase staining for differentiation of each microfilaria species. In the modified Knott's test, 74 of 506 dogs (14.6%) were microfilaria positive, and the prevelance of each species of microfilaria was 90.5% (67 of 74 samples) for D. immitis, 5.4% (4 of 74 samples) for duplicate infection with D. immitis and D. repens and 4.1% (3 of 74 samples) for mixed infection with D. immitis, D. repens and A. dracunculoides. It was considered that the paying attention to the existence not only D. immitis but also other microfilariae were needed in canine filariasis.

• Advances in Traditional Medicine
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• 제5권2호
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• pp.110-116
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• 2005

The effect of alcohol and aqueous extracts of the leaves and stem of Pluchea lanceolata on the spontaneous movements of both the whole worm and the nerve muscle preparation of Setaria cervi, and on the survival of microfilariae in vitro was studied. Alcohol and aqueous extracts of the leaves and stem of P. lanceolata caused the inhibition of spontaneous movements of the whole worm and the nerve muscle preparation of S. cervi, characterized by short lasting small increase in tone and amplitude of contractions followed by paralysis. The concentrations required to inhibit the movements of whole worm and nerve muscle preparations for alcohol extract were 200 and $25\;{\mu}g/ml$, and for aqueous extract were 250 and $100\;{\mu}g/ml$, respectively, suggesting a cuticular permeability barrier. Both the extracts (alcohol and aqueous) caused death of microfilariae in vitro, $LC_{50}$ and $LC_{90}$ being 12 and 18 ng/ml for alcohol extract and 25 and 40 ng/ml for aqueous extract, respectively.

• 대한수의학회지
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• 제40권2호
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• pp.339-343
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• 2000

Isolation and identification, prevalence and density of Setaria microfilariae(mf) were investigated in the blood of 406 cattle slaughtered at the abatoirs in Yungnam province from February 1999 to March 2000. Nine ml of blood from the jugular vein was collected in a test tube including EDTA respectively. The blood was mixed(10 : 1) with 0.2% Saponin in saline solution. This mixture was diluted again(1 : 10) with saline solution and stirred. This diluted mixture was filtered using Millipore membrane filter with a pore size of $3.0{\mu}m$. Mf were recovered from the membrane in saline solution. Mf isolated were identified as Setaria digitata on the basis of morphologic characteristics. Infection rate of the mf of total 406 cattle was revealed as 6.40%. Seasonal prevalences of the mf were presented 9.46%(7/74) in Autumn, 7.05%(11/156) in Summer, 4.65%(4/86) in Winter and 4.44%(4/90) in Spring respectively. Average density of the mf of every 9ml blood in 26 positive cattle was revealed as 170(2-641) worms. Seasonal densities of the mf of 9ml blood were presented average 361(160-641) worms in Autumn, 205(16-620) worms in Summer, 108(6-204) worms in Spring, 6(2-11) worms in Winter respectively.